RDB13480 - pCS2P-tyr-mCherry-donor

Please review the QC test results indicated by check

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pCS2P-tyr-mCherry-donor (#RDB13480)

Donor vector for integrating mCherry into zebrafish tyrosinase locus.

Clone info.	Donor vector for integrating mCherry into zebrafish tyrosinase locus.
Comment	CMV promoter of the pCS2p vector was removed by HindIII-SalI digestion.
Vector backbone	pCS2p (plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	Zebrafish tyrosinase genomic DNA Discosoma sp. Red fluorescent protein (RFP) cDNA Aquorea victoria Green flurescent protein (GFP)
Depositor\|Developer	Kawahara, Atsuo \| Hisano, Yu \|

Sequence	RDB13480z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Sci. Rep., 5, 8841 (2015))

Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Sci. Rep., 5, 8841 (2015))
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.

必要書類	提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Sci Rep., 5, 8841 (2015))
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13480	pCS2P-tyr-mCherry-donor	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCS2P-tyr-mCherry-donor was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13480).

Reference section:

Hisano, Y., Sakuma, T., Nakade, S., Ohga, R., Ota, S., Okamoto, H., Yamamoto, T., Kawahara, A., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13480_A5I9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNLF1 (Pr0166) Region: SV40p(A),mCherry,homology arm,eGFP-gRNA,homology arm Sequence file: RDB13480_A5I9b.seq
>13480_13480_A5I9_pAxCALNL_F1_F04_16.ab1 1 ACATGATTAT ACCTTATCAT GTCTGGATCT ACGTAATACG ACTCACTATA GGGCTGCAGA 61 ATCTAGATCA CTTATACAGC TCGTCCATGC CTCCAGTTGA ATGTCTGCCC TCGGCGCGCT 121 CATACTGCTC CACAATTGTG TAATCCTCGT TGTGGGAAGT GATGTCCAGT TTAATATTCA 181 CGTTGTAGGC TCCAGGCAGC TGCACGGGTT TCTTAGCTTT ATAGGTTGTC TTCACCTCTG 241 CATCGTAGTG ACCGCCGTCT TTCAGCTTCA GTCTCTGTTT GATCTCTCCC TTCAGGGCGC 301 CATCCTCAGG ATACATGCGC TCTGAGGAAG CCTCCCAGCC CATAGTTTTC TTCTGCATCA 361 CTGGGCCGTC GGAAGGAAAA TTGGTTCCGC GCAGTTTCAC CTTGTAGATG AACTCTCCAT 421 CCTGCAGAGA GCTGTCCTGT GTCACAGTCA CCACTCCACC ATCCTCAAAG TTCATCACCC 481 TCTCCCATTT AAAGCCCTCA GGGAAAGACA GCTTCAGATA ATCGGGGATG TCAGCTGGGT 541 GTTTCACATA TGCCTTGGAA CCGTACATAA ACTGTGGAGA CAGAATGTCC CATGCGAAAG 601 GCAGGGGGCC TCCCTTGGTC ACTTTCAGCT TGGCGGTCTG TGTGCCCTCG TATGGCCTAC 661 CCTCGCCCTC TCCCTCGATC TCGAACTCAT GTCCATTCAC GGAACCCTCC ATGTGCACTT 721 TAAATCTCAT GAACTCCTTA ATGATGGCCA TGTTGTCTTC CTCTCCTTTA GACACCATGC 781 CGGGCCAGAC TGGACAGCAG CGTTTGGACT GGAGGACTTC CGTAGGTGGC ATCGCCCTCG 841 CCGAACCCTC GACCTGACTG GACGCCGCAC ACGGAGCCGT CGGTCACCTA AATCAAAGCT 901 TCGACTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC 961 TGAATGGCCG AATGGGACGC GCCCTGTAGC GGCGCATTAA AGCGCGGCGG TGTGGGTGTT 1021 TACGCGCAGC GTGACCGCTA ACACTTTGCA GCGCCCTAGC GCCCCGCTCT TTCGCTTTCT 1081 TCCCTTCCCT TTTCTCGCCA CGGATCGTCA GCTTTCCCGG TCAAGCTCTT AAATTCGGGG 1141 GGACATCCCC T //

Primer: pAxCALNLF1 (Pr0166)
Region: SV40p(A),mCherry,homology arm,eGFP-gRNA,homology arm
Sequence file: RDB13480_A5I9b.seq check

>13480_13480_A5I9_pAxCALNL_F1_F04_16.ab1
    1 ACATGATTAT ACCTTATCAT GTCTGGATCT ACGTAATACG ACTCACTATA GGGCTGCAGA
   61 ATCTAGATCA CTTATACAGC TCGTCCATGC CTCCAGTTGA ATGTCTGCCC TCGGCGCGCT
  121 CATACTGCTC CACAATTGTG TAATCCTCGT TGTGGGAAGT GATGTCCAGT TTAATATTCA
  181 CGTTGTAGGC TCCAGGCAGC TGCACGGGTT TCTTAGCTTT ATAGGTTGTC TTCACCTCTG
  241 CATCGTAGTG ACCGCCGTCT TTCAGCTTCA GTCTCTGTTT GATCTCTCCC TTCAGGGCGC
  301 CATCCTCAGG ATACATGCGC TCTGAGGAAG CCTCCCAGCC CATAGTTTTC TTCTGCATCA
  361 CTGGGCCGTC GGAAGGAAAA TTGGTTCCGC GCAGTTTCAC CTTGTAGATG AACTCTCCAT
  421 CCTGCAGAGA GCTGTCCTGT GTCACAGTCA CCACTCCACC ATCCTCAAAG TTCATCACCC
  481 TCTCCCATTT AAAGCCCTCA GGGAAAGACA GCTTCAGATA ATCGGGGATG TCAGCTGGGT
  541 GTTTCACATA TGCCTTGGAA CCGTACATAA ACTGTGGAGA CAGAATGTCC CATGCGAAAG
  601 GCAGGGGGCC TCCCTTGGTC ACTTTCAGCT TGGCGGTCTG TGTGCCCTCG TATGGCCTAC
  661 CCTCGCCCTC TCCCTCGATC TCGAACTCAT GTCCATTCAC GGAACCCTCC ATGTGCACTT
  721 TAAATCTCAT GAACTCCTTA ATGATGGCCA TGTTGTCTTC CTCTCCTTTA GACACCATGC
  781 CGGGCCAGAC TGGACAGCAG CGTTTGGACT GGAGGACTTC CGTAGGTGGC ATCGCCCTCG
  841 CCGAACCCTC GACCTGACTG GACGCCGCAC ACGGAGCCGT CGGTCACCTA AATCAAAGCT
  901 TCGACTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC
  961 TGAATGGCCG AATGGGACGC GCCCTGTAGC GGCGCATTAA AGCGCGGCGG TGTGGGTGTT
1021 TACGCGCAGC GTGACCGCTA ACACTTTGCA GCGCCCTAGC GCCCCGCTCT TTCGCTTTCT
1081 TCCCTTCCCT TTTCTCGCCA CGGATCGTCA GCTTTCCCGG TCAAGCTCTT AAATTCGGGG
1141 GGACATCCCC T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Hisano, Y., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433. [link to RRC of NBRP]