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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCS2P-tyr-mCherry-donor

Donor vector for integrating mCherry into zebrafish tyrosinase locus.

Catalog number RDB13480
Resource name pCS2P-tyr-mCherry-donor
Clone info. Donor vector for integrating mCherry into zebrafish tyrosinase locus.
CMV promoter of the pCS2p vector was removed by HindIII-SalI digestion.
Vector backbone pCS2p (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Zebrafish tyrosinase genomic DNA Discosoma sp. Red fluorescent protein (RFP) cDNA Aquorea victoria Green flurescent protein (GFP)
Depositor|Developer Kawahara, Atsuo | Hisano, Yu |
 
Other clones in our bank Zebrafish tyr |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Sci. Rep., 5, 8841 (2015))
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Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13480 pCS2P-tyr-mCherry-donor DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB13480z.seq

Original reference

original Hisano, Y., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13480_A5I9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNLF1
Region: SV40p(A),mCherry,homology arm,eGFP-gRNA,homology arm
Sequence file: RDB13480_A5I9b.seq check
>13480_13480_A5I9_pAxCALNL_F1_F04_16.ab1
    1 ACATGATTAT ACCTTATCAT GTCTGGATCT ACGTAATACG ACTCACTATA GGGCTGCAGA
   61 ATCTAGATCA CTTATACAGC TCGTCCATGC CTCCAGTTGA ATGTCTGCCC TCGGCGCGCT
  121 CATACTGCTC CACAATTGTG TAATCCTCGT TGTGGGAAGT GATGTCCAGT TTAATATTCA
  181 CGTTGTAGGC TCCAGGCAGC TGCACGGGTT TCTTAGCTTT ATAGGTTGTC TTCACCTCTG
  241 CATCGTAGTG ACCGCCGTCT TTCAGCTTCA GTCTCTGTTT GATCTCTCCC TTCAGGGCGC
  301 CATCCTCAGG ATACATGCGC TCTGAGGAAG CCTCCCAGCC CATAGTTTTC TTCTGCATCA
  361 CTGGGCCGTC GGAAGGAAAA TTGGTTCCGC GCAGTTTCAC CTTGTAGATG AACTCTCCAT
  421 CCTGCAGAGA GCTGTCCTGT GTCACAGTCA CCACTCCACC ATCCTCAAAG TTCATCACCC
  481 TCTCCCATTT AAAGCCCTCA GGGAAAGACA GCTTCAGATA ATCGGGGATG TCAGCTGGGT
  541 GTTTCACATA TGCCTTGGAA CCGTACATAA ACTGTGGAGA CAGAATGTCC CATGCGAAAG
  601 GCAGGGGGCC TCCCTTGGTC ACTTTCAGCT TGGCGGTCTG TGTGCCCTCG TATGGCCTAC
  661 CCTCGCCCTC TCCCTCGATC TCGAACTCAT GTCCATTCAC GGAACCCTCC ATGTGCACTT
  721 TAAATCTCAT GAACTCCTTA ATGATGGCCA TGTTGTCTTC CTCTCCTTTA GACACCATGC
  781 CGGGCCAGAC TGGACAGCAG CGTTTGGACT GGAGGACTTC CGTAGGTGGC ATCGCCCTCG
  841 CCGAACCCTC GACCTGACTG GACGCCGCAC ACGGAGCCGT CGGTCACCTA AATCAAAGCT
  901 TCGACTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC
  961 TGAATGGCCG AATGGGACGC GCCCTGTAGC GGCGCATTAA AGCGCGGCGG TGTGGGTGTT
 1021 TACGCGCAGC GTGACCGCTA ACACTTTGCA GCGCCCTAGC GCCCCGCTCT TTCGCTTTCT
 1081 TCCCTTCCCT TTTCTCGCCA CGGATCGTCA GCTTTCCCGG TCAAGCTCTT AAATTCGGGG
1141 GGACATCCCC T
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hisano, Y., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433.

2021.03.11

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