Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCS2P-tyr-mCherry-donor

Donor vector for integrating mCherry into zebrafish tyrosinase locus.

Catalog number RDB13480
Resource name pCS2P-tyr-mCherry-donor
Clone info. Donor vector for integrating mCherry into zebrafish tyrosinase locus.
Comment CMV promoter of the pCS2p vector was removed by HindIII-SalI digestion.
Vector backbone pCS2p (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Zebrafish tyrosinase genomic DNA Discosoma sp. Red fluorescent protein (RFP) cDNA Aquorea victoria Green flurescent protein (GFP)
Depositor|Developer Kawahara, Atsuo | Hisano, Yu |
Other clones in our bank
External Database
Zebrafish tyrosinase Discosoma sp. Red fluorescent protein (RFP)
    Aquorea victoria Green flurescent protein (GFP)
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Sci. Rep., 5, 8841 (2015))
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
          Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Sci Rep., 5, 8841 (2015))
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
          備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13480 pCS2P-tyr-mCherry-donor DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB13480z.seq

          Original reference

          original Hisano, Y., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Genome Editing (English text)
          Featured content Genome Editing (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13480_A5I9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCALNLF1
          Region: SV40p(A),mCherry,homology arm,eGFP-gRNA,homology arm
          Sequence file: RDB13480_A5I9b.seq check
          >13480_13480_A5I9_pAxCALNL_F1_F04_16.ab1
              1 ACATGATTAT ACCTTATCAT GTCTGGATCT ACGTAATACG ACTCACTATA GGGCTGCAGA
             61 ATCTAGATCA CTTATACAGC TCGTCCATGC CTCCAGTTGA ATGTCTGCCC TCGGCGCGCT
            121 CATACTGCTC CACAATTGTG TAATCCTCGT TGTGGGAAGT GATGTCCAGT TTAATATTCA
            181 CGTTGTAGGC TCCAGGCAGC TGCACGGGTT TCTTAGCTTT ATAGGTTGTC TTCACCTCTG
            241 CATCGTAGTG ACCGCCGTCT TTCAGCTTCA GTCTCTGTTT GATCTCTCCC TTCAGGGCGC
            301 CATCCTCAGG ATACATGCGC TCTGAGGAAG CCTCCCAGCC CATAGTTTTC TTCTGCATCA
            361 CTGGGCCGTC GGAAGGAAAA TTGGTTCCGC GCAGTTTCAC CTTGTAGATG AACTCTCCAT
            421 CCTGCAGAGA GCTGTCCTGT GTCACAGTCA CCACTCCACC ATCCTCAAAG TTCATCACCC
            481 TCTCCCATTT AAAGCCCTCA GGGAAAGACA GCTTCAGATA ATCGGGGATG TCAGCTGGGT
            541 GTTTCACATA TGCCTTGGAA CCGTACATAA ACTGTGGAGA CAGAATGTCC CATGCGAAAG
            601 GCAGGGGGCC TCCCTTGGTC ACTTTCAGCT TGGCGGTCTG TGTGCCCTCG TATGGCCTAC
            661 CCTCGCCCTC TCCCTCGATC TCGAACTCAT GTCCATTCAC GGAACCCTCC ATGTGCACTT
            721 TAAATCTCAT GAACTCCTTA ATGATGGCCA TGTTGTCTTC CTCTCCTTTA GACACCATGC
            781 CGGGCCAGAC TGGACAGCAG CGTTTGGACT GGAGGACTTC CGTAGGTGGC ATCGCCCTCG
            841 CCGAACCCTC GACCTGACTG GACGCCGCAC ACGGAGCCGT CGGTCACCTA AATCAAAGCT
            901 TCGACTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC
            961 TGAATGGCCG AATGGGACGC GCCCTGTAGC GGCGCATTAA AGCGCGGCGG TGTGGGTGTT
           1021 TACGCGCAGC GTGACCGCTA ACACTTTGCA GCGCCCTAGC GCCCCGCTCT TTCGCTTTCT
           1081 TCCCTTCCCT TTTCTCGCCA CGGATCGTCA GCTTTCCCGG TCAAGCTCTT AAATTCGGGG
          1141 GGACATCCCC T
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Hisano, Y., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl