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pTRE-G1

Expression plasmid of Dox-inducible hu-lgfp in mammalian cells.

Catalog number RDB13460
Resource name pTRE-G1
Clone info. Expression plasmid of Dox-inducible hu-lgfp (AcGFP) in mammalian cells. G418 resistance.
Comment hu-lgfp: The AcGFP1 gene, changed its all codons to the lowest usage in H. sapiens (accession number, LC018337).
Vector backbone pTRE-Tight (plasmid)
Size of vector backbone 3.3 kb
Selectable markers G418 (mammalian cell)
Gene/insert name Aequorea coerulescens green fluorescent protein (GFP) cDNA
Reference of insert sequence LC018352 (DDBJ accession number)
Depositor|Developer Kobayashi, Hideki |
Other clones in our bank
External Database
Aequorea coerulescens green fluorescent protein (GFP)
            Reference sequence
               LC018337
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015)

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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015)。
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            RDB13460 pTRE-G1 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pTRE-G1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13460).

            Reference section:

            Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

            References and tips

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849. [link to RRC of NBRP]

            2023.06.15

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