Expression plasmid of Dox-inducible hu-lgfp in mammalian cells.
Clone info. | Expression plasmid of Dox-inducible hu-lgfp (AcGFP) in mammalian cells. G418 resistance. |
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Comment | hu-lgfp: The AcGFP1 gene, changed its all codons to the lowest usage in H. sapiens (accession number, LC018337). |
Vector backbone | pTRE-Tight (plasmid) |
Size of vector backbone | 3.3 kb |
Selectable markers | G418 (mammalian cell) |
Gene/insert name | Aequorea coerulescens green fluorescent protein (GFP) cDNA |
Reference of insert sequence | LC018352 (DDBJ accession number) |
Depositor|Developer | Kobayashi, Hideki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB13460 | pTRE-G1 | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pTRE-G1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13460). |
Reference section:
Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849. [link to RRC of NBRP] |
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