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pLGFP delta 2

Bacterial expression plasmid of lgfp delta 2, IPTG-inducible. ColE1 origin and ampicillin resistance.

Catalog number RDB13452
Resource name pLGFP delta 2
Clone info. Bacterial expression plasmid of lgfp delta 2, IPTG-inducible. ColE1 origin and ampicillin resistance.
lgfp delta 2: The modified lgfp gene, deleted 357 bp (50%) from C-terminal (LC018333)
Vector backbone pTAK132 (plasmid)
Size of vector backbone 4.6 kb
Selectable markers ampicillin (E. coli)
Gene/insert name Aequorea victoria green fluorescent protein (GFP) cDNA
Reference of nucleotide sequence LC018340 (DDBJ accession number)
Depositor|Developer Kobayashi, Hideki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015)

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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13452 pLGFP delta 2 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849.

2021.08.31

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