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pLGFPm

Bacterial expression plasmid of lgfp, IPTG-inducible. p15A origin and ampicillin resistance.

Catalog number RDB13449
Resource name pLGFPm
Clone info. Bacterial expression plasmid of lgfp, IPTG-inducible. p15A origin and ampicillin resistance.
lgfp: The gfpmut3 gene, changed its all codons to the lowest usage in E. coli (accession number, LC018331).
Vector backbone pTAK132 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers ampicillin (E. coli)
Gene/insert name Aequorea victoria green fluorescent protein (GFP) cDNA
Reference of nucleotide sequence LC018342 (DDBJ accession number)
Depositor|Developer Kobayashi, Hideki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015)

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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13449 pLGFPm Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849.

2021.08.31

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