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pLGFP (#RDB13447)

Bacterial expression plasmid of lgfp, IPTG-inducible. ColE1 origin and ampicillin resistance.

Clone info. Bacterial expression plasmid of lgfp, IPTG-inducible. ColE1 origin and ampicillin resistance.
Comment lgfp: The gfpmut3 gene, changed its all codons to the lowest usage in E. coli (accession number, LC018331).
Vector backbone pTAK132 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers ampicillin (E. coli)
Gene/insert name Aequorea victoria green fluorescent protein (GFP) cDNA
Reference of insert sequence LC018338 (DDBJ accession number)
Depositor|Developer Kobayashi, Hideki |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015)

提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13447 pLGFP Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pLGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13447).

Reference section:

Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849. [link to RRC of NBRP]