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pcDNA3.1/hGnT-V-myc-his

Expression vector of human GnT-V in mammalian cell

Catalog number RDB13428
Resource name pcDNA3.1/hGnT-V-myc-his
Clone info. Expression vector of human GnT-V in mammalian cell.
Comment Exression was confirmed by the depositor with Western blotting and enzymatic activity asssay.
Vector backbone pcDNA3.1/Myc-His version C (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human MGAT5 cDNA
Depositor|Developer Taniguchi, Naoyuki | Kizuka, Yasuhiko |
Other clones in our bank
External Database
Human MGAT5
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem, 288 (39), 27912-27926 (2013))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem, 288 (39), 27912-27926 (2013))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB13428 pcDNA3.1/hGnT-V-myc-his DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3.1/hGnT-V-myc-his was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13428).

          Reference section:

          Korekane, H., Park, J.Y., Matsumoto, A., Nakajima, K., Takamatsu, S., Ohtsubo, K., Miyamoto, Y., Hanashima, S., Kanekiyo, K., Kitazume, S., Yamaguchi, Y., Matsuo, I., Taniguchi, N., Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb). J. Biol. Chem. 288 (39): 27912-27926 (2013). PMID 23960081.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13428_A6Dqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'
          Sequence file: RDB13428_A6Dqa.seq check
          >D02340A2_A6Dq_3_CMV_Forward_H01_22.ab1
              1 NNNNNNNNNN NNNNTNGNNG CNNTGGGCGG TANGCGTGTA CGGTGGGNNG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCATGGCTCT CTTCACTCCG
            181 TGGAAGTTGT CCTCTCAGAA GCTGGGCTTT TTCCTGGTGA CTTTTGGCTT CATTTGGGGT
            241 ATGATGCTTC TGCACTTTAC CATCCAGCAG CGAACTCAGC CTGAAAGCAG CTCCATGCTG
            301 CGCGAGCAGA TCCTGGACCT CAGCAAAAGG TACATCAAGG CACTGGCAGA AGAAAACAGG
            361 AATGTGGTGG ATGGGCCATA CGCTGGAGTC ATGACAGCTT ATGATCTGAA GAAAACCCTT
            421 GCTGTGTTAT TAGATAACAT TTTGCAGCGC ATTGGCAAGT TGGAGTCGAA GGTGGACAAT
            481 CTTGTTGTCA ATGGCACCGG AACAAACTCA ACCAACTCCA CTACAGCTGT TCCCAGCTTG
            541 GTTGCACTTG AGAAAATTAA TGTGGCAGAT ATCATTAACG GAGCTCAAGA AAAATGTGTA
            601 TTGCCTCCTA TGGACGGCTA CCCTCACTGT GAGGGAAAGA TCAAGTGGAT GAAAGACATG
            661 TGGCGTTCAG ATCCCTGCTA CGCAGACTAT GGAGTGGATG GATCCACCTG CTCTTTTTTT
            721 ATTTACCTCA GTGANGTTGA AAATTGGTGT CCTCATTTAC CTTGGANNNC NAAAAATCCC
            781 TACGAAGAAN CTGATCATAA TTCATTGGCG GAAATTCNNA CAGNNTTTTA ATANTCTCTA
            841 CAGNATGNNG NAAAANCNTG NANNNANNNC GGNNGNNNNN NNNNANNNNN NCNGNNNNNA
          901 NNNNNNNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,6xHis,myc,insert 3'
          Sequence file: RDB13428_A6Dqb.seq check
          >13428_13428_B2EgF_3_BGH_rev2_A02_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNGNNN GGGGGNNGGG CANACNACAG ATGGCTGGCA ACTAGAAGGC
             61 ACAGTCGAGG CTGATCAGCG GGTTTAAACT CAATGGTGAT GGTGATGATG ACCGGTATGC
            121 ATATTCAGAT CCTCTTCTGA GATGAGTTTT TGTTCGAATG GGTGACCTCG AGCGGCCGCC
            181 ACTGTGCTGG ATAGGCAGTC TTTGCAGAGA GCCACCTGGC CCTTGATGAA GTCCCGGCAG
            241 GGGCAGACCC TCTGGTGCCT GGGGTGGGCG CCTGCACAGC TGAAGAGCAG GAGGTCACCT
            301 TGAAACACAC AGTGCTTATT CTTAGGGTCA AAGGAGGGCA CCAGGATGTC CTTGGCCAGC
            361 TCTGAGCTTT GGCAGGTCAC CTTGTACTTC AGCATGTCCT TGTCCTTGTT GAGGTGCTGG
            421 AAGAAAGAAG GCTCGCAGAT GAGCTGGCTC TCCTGGCACA CCTGCTTGCA GGACTGCCCG
            481 GGCTCAGCAA GCTTGACCTG TAGGGCGCTG AGGGGTGGCC ACATCACTTG CCCATGGCAG
            541 AAGTCCTGTT TTTCAATGAA AGCATTGATT CTCTGTAGCA TCCCCTCGCA CGTAAATTCA
            601 TATGGCATGT ATGGCTCAAT CTTCTGATTT AAAATTGCTT TCACTGCATC CTCTACTTCC
            661 TCCTGATTGT TGAGGTCAAC AGTCCACACA TGTGGCCGCC CGATGAAAAC TTCAGCGTAA
            721 GGATGCTGGG ATGTCAGCTC TCTCAGAGTT GGCTTGCCAA TGAAAAAGTC TGTGTTTTTG
            781 CTGCTTTTGG GTGGGTTGAA CTTGGGANTC AGAAAAGCAC ATCNNTTTGC GATAGCTTCC
            841 ANNGGAGCTG GGCCCTCGTA ANGNAACCCN AGTCCNACAA ACAACTTGGT TTCTCGAANA
            901 ANGAACTGCA GGNCCCGTCN ACNGNNNANA NNNNGGTTTT TCNCGNNNCT GGNNATANTC
            961 TTTGTGCTGN ANCCNTAAAC NNNNGCATGC NNNTNCANGN ATGNGNGNNT NANGNCCNAN
          1021 NANNNNNNCN TNANNNNNNN NNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB13428_A6Dqc.seq check
          >D02340A2_A6Dq_3_SV40pro_F_G05_20.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTGNNGN NGCTTTTTTG GNNGCCTANG CTTTTGCNAA
             61 AAGCTCCCGG GNNCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
            121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
            661 CCCGACGGCG ANGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
            781 CNNGNCATAG CGTTGGCTAC NCGNGATATT GCTGAAGANC TTGGCGGCNA ATGGGCTGAN
            841 CGCTNCCNCN NGCNTTACNG NANCGCCGCN CCCGANNCNC ANCNNNNCGC CNTCNATCGN
          901 NNTCTTGNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Korekane, H., Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb). J. Biol. Chem. 288 (39): 27912-27926 (2013). PMID 23960081.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl