RDB13379 - pGF-mSox2-ONL

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pGF-mSox2-ONL (#RDB13379)

Lentiviral reporter plasmid of mouse Sox2 by the Orange Nano-lantern.

Clone info.	Lentiviral reporter plasmid of mouse Sox2 by the Orange Nano-lantern.
Comment	Expression was confirmed by the depositor with transfection into murine ES cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Reporter gene was deleted from the original vector. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone	pGreenFire1 (Plasmid)
Size of vector backbone	9.4 kb
Growth conditions	LB (+Amp)
Gene/insert name	mouse Sox2 genomic DNA
Depositor\|Developer	Okada, Yasushi \| Takai, Akira \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13379	pGF-mSox2-ONL	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGF-mSox2-ONL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13379).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13379_A5E8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: env_F () Sequence file: RDB13379_A5E8a.seq
>13379_13379_A5E8_3_env_F_E09_15.ab1 1 GGCGTGCAGG ATCCATTCGA TTAGTGACGG ATCTGCGACG GTATCGGTTA ACTTTTAAAA 61 GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG 121 ACATACAAAC TAAAGAATTA CAAAAACAAA TTACAAAATT CAAAATTTTA TCGATGAATT 181 CTAATTAATG CAGAGACTCT AAAAGAATTT CCCGGGCTCG GGCAGCCATT GTGATGCATA 241 TAGGATTATT CACGTGGTAA TGTAATTAAT GCAGAGACTC TAAAAGAATT TCCCGGGCTC 301 GGGCAGCCAT TGTGATGCAT ATAGGATTAT TCACGTGGTA ATGCCAGTCC AAGCTAGGCA 361 GGTTCCCCTC TAATTAATGC AGAGACTCTA AAAGAATTTC CCGGGCTCGG GCAGCCATTG 421 TGATGCATAT AGGATTATTC ACGTGGTAAT GAGCACAGTC GACAGTTCTT GCGGATCCAC 481 CATGGTGAGC GTGATCAAGC CCGAGATGAA GATGAGGTAC TACATGGACG GCTCCGTCAA 541 TGGGCATGAG TTCACAATCG AGGGTGAGGG CACAGGCAGA CCTTACGAGG GACATCAGGA 601 GATGACACTG CGCGTCACAA TGGCCGAGGG CGGGCCAATG CCTTTCGCCT TCGACCTGGT 661 GTCCCACGTG TTCTGTTACG GCCACAGAGT TTTTACTAAA TATCCAGAAG AGATCCCAGA 721 CTATTTCAAG CAGGCCTTTC CTGAGGGCCT GTCCTGGGAG AGGTCCCTGG AGTTCGAGGA 781 CGGCGGCTCC GCCTCCGTGA GCGCCCACAT CAGCCTGAGG GGCAACACCT TCTACCACAA 841 GTCCAAGTTC ACCGGCGTGA ACTTCCCCGC CGACGGCCCC ATCATGCAGA ACCAGAGCGT 901 GGACTGGGAG CCCTCCACCG AGAAGATCAC CGCCAGCGAC GGCGTGCTGA AAGGGCGACG 961 TGACCATGTA CCTGAAGCTG GAGGGCGGCG GCAACCACAA GTGCCAGATG AAGACCACCC 1021 TACACGGCCG CCCAAGGAGA TCCCTGGAGA TGCCCGGCGA CCACTACATC GGGCCACAGG 1081 CTGCTTGAGA AGACCGAGGG GCAACATTCA CGAGCAAGTG AAGAACGGCC GTGGACCACC 1141 TCCGGTACCA AGGGTGGTTA ACCGAA //
Primer: ONL-M_R () Sequence file: RDB13379_A5E8b.seq
>13379_13379_A9D8F_3_ONL-M_R_A08_02_ABI24.ab1 1 CTTTAGTTGC CTCGGTCTTC TCACCAGCCT GTGGCCGATG TAGTGGTCGC CGGGCATCTC 61 CAGGATCTCC TTGGCGGCCT TGTAGGTGGT CTTCATCTGG CACTTGTGGT TGCCGCCGCC 121 CTCCAGCTTC AGGTACATGG TCACGTCGCC CTTCAGCACG CCGTCGCTGG CGGTGATCTT 181 CTCGGTGGAG GGCTCCCAGT CCACGCTCTG GTTCTGCATG ATGGGGCCGT CGGCGGGGAA 241 GTTCACGCCG GTGAACTTGG ACTTGTGGTA GAAGGTGTTG CCCCTCAGGC TGATGTGGGC 301 GCTCACGGAG GCGGAGCCGC CGTCCTCGAA CTCCAGGGAC CTCTCCCAGG ACAGGCCCTC 361 AGGAAAGGCC TGCTTGAAAT AGTCTGGGAT CTCTTCTGGA TATTTAGTAA AAACTCTGTG 421 GCCGTAACAG AACACGTGGG ACACCAGGTC GAAGGCGAAA GGCATTGGCC CGCCCTCGGC 481 CATTGTGACG CGCAGTGTCA TCTCCTGATG TCCCTCGTAA GGTCTGCCTG TGCCCTCACC 541 CTCGATTGTG AACTCATGCC CATTGACGGA GCCGTCCATG TAGTACCTCA TCTTCATCTC 601 GGGCTTGATC ACGCTCACCA TGGTGGATCC GCAAGAACTG TCGACTGTGC TCATTACCAC 661 GTGAATAATC CTATATGCAT CACAATGGCT GCCCGAGCCC GGGAAATTCT TTTAGAGTCT 721 CTGCATTAAT TAGAGGGGAA CCTGCCTAGC TTGGACTGGC ATTACCACGT GAATAATCCT 781 ATATGCATCA CAATGGCTGC CCGAGCCCGG GAAATTCTTT TAGAGTCTCT GCATTAATTA 841 CATTACCACG TGAATAATCC TATATGCAAT CACAATGGCT GCCCGAGCCC GGGAAATTTC 901 TTTTAGAGTC TCTGCATTAA TTAGAATCAT CGATAAAATT TTTGAATTTT GTAATTTGGT 961 TTTTGGAAAT TCTTTAGTTT GTATGTCTGG TTGCTATTAT GTCTACTATT CTTTCCCCTG 1021 CACTGTACCC CCCAATCCCC CCCTTTTCTT TAAAAGTTAA ACCGAATACC GGTCCAAGAA 1081 TCCGGTTTCA CTAAGTCGGA ATGGAATTCT AGATCTCTAT CTCATCATCT TCTCAACGAT 1141 CCTGTATCTA TGTCGAATTA TCCCAGTGCC GGGGCCCGTA TGATTG //
Primer: mKO2-M_F () Sequence file: RDB13379_A5E8c.seq
>13379_13379_A9D8F_3_mKO2-M_F_C04_03_ABI08.ab1 1 TGGGCGGGCG GCCAACAACA AGGTGGCCAG ATGAAGACCA CCTACAAGGC CGCCAAGGAG 61 ATCCTGGAGA TGCCCGGCGA CCACTACATC GGCCACAGGC TGGTGAGGAA GACCGAGGGC 121 AACATCACCG AGCAGGTGGA GGACGCCGTG GCCCACTCCG GTACCAAGGT GTACGACCCC 181 GAGCAACGCA AACGCATGAT CACTGGGCCT CAGTGGTGGG CTCGCTGCAA GCAAATGAAC 241 GTGCTGGACT CCTTCATCAA CTACTATGAT TCCGAGAAGC ACGCCGAGAA CGCCGTGATT 301 TTTCTGCATG GTAACGCTAC CTCCAGCTAC CTGTGGAGGC ACGTCGTGCC TCACATCGAG 361 CCCGTGGCTA GATGCATCAT CCCTGATCTG ATCGGAATGG GTAAGTCCGG CAAGAGCGGG 421 AATGGCTCAT ATCGCCTCCT GGATCACTAC AAGTACCTCA CCGCTTGGTT CGAGCTGCTG 481 AACCTTCCAA AGAAAATCAT CTTTGTGGGC CACGACTGGG GGAGCGCTCT GGCCTTTCAC 541 TACGCCTACG AGCACCAAGA CAGGATCAAG GCCATCGTCC ATATGGAGAG TGTCGTGGAC 601 GTGATCGAGT CCTGGATGGG GTGGCCTGAC ATCGAGGAGG AGCTGGCCCT GATCAAGAGC 661 GAAGAGGGCG AGAAAATGGT GCTTGAGAAT AACTTCTTCG TCGAGACCCT GTTGCCAAGC 721 AAGATCATGC GGGAAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG CCATTCAAGG 781 AAGAAGGGCG AGGTTAGACG GCCTACCCTC TCCTGGCCTC GCGAGATCCC CTCTCTTAAG 841 GGAAGGCAAA GCCCGAACGT CGTCCAGATT GTTCCGCAAC TAACAACGGC CTACCTTCCG 901 GCCAACCAAC GATCTTGCCT AAACTGTTTC ATCAAGTCCA ACCCCTGGGT TTCTTTTCCA 961 CGCTTATTTG TCGAGGGGAG CTAAAAAAGT CCCTAGAGCC GAAGTTTTGT GAAGGTTGAA 1021 CGGGGCCCCA ACTTCCTACC AGAAGGACGC TCGCAGAATA TAAATGGGGA AAGTACCACT 1081 CAG //
Primer: RLuc8.6-M_F () Sequence file: RDB13379_A5E8d.seq
>13379_13379_A9D8F_3_RLuc8.6-M_F_H05_08_ABI08.ab1 1 CGTAGTGGGG GGGGGCCTGA CATCGAGGAG GAGCTGGCCC TGATCAAGAG CGAAGAGGGC 61 GAGAAAATGG TGCTTGAGAA TAACTTCTTC GTCGAGACCC TGTTGCCAAG CAAGATCATG 121 CGGAAACTGG AGCCTGAGGA GTTCGCTGCC TACCTGGAGC CATTCAAGGA GAAGGGCGAG 181 GTTAGACGGC CTACCCTCTC CTGGCCTCGC GAGATCCCTC TCGTTAAGGG AGGCAAGCCC 241 GACGTCGTCC AGATTGTCCG CAACTACAAC GCCTACCTTC GGGCCAGCGA CGATCTGCCT 301 AAGCTGTTCA TCGAGTCCGA CCCTGGGTTC TTTTCCAACG CTATTGTCGA GGGAGCTAAG 361 AAGTTCCCTA ACACCGAGTT CGTGAAGGTG AAGGGCCTCC ACTTCCTCCA GGAGGACGCT 421 CCAGATGAAA TGGGTAAGTA CATCAAGAGC TTCGTGGAGC GCGTGCTGAA GAACGAGCAG 481 TAACTCGACT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATC //
Primer: pUC-M13_-100 () Sequence file: RDB13379_A5E8e.seq
>13379_13379_A9D8F_3_pUC-M13_-100_C08_08_ABI24.ab1 1 GCCGTCAGGC GATTAGTTGG GTACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC 61 GGCCAGTGCC AAGCTGACGC GTGTAGTCTT ATGCAATACT CTTGTAGTCT TGCAACATGG 121 TAACGATGAG TTAGCAACAT GCCTTACAAG GAGAGAAAAA GCACCGTGCA TGCCGATTGG 181 TGGAAGTAAG GTGGTACGAT CGTGCCTTAT TAGGAAGGCA ACAGACGGGT CTGACATGGA 241 TTGGACGAAC CACTGAATTG CCGCATTGCA GAGATATTGT ATTTAAGTGC CTAGCTCGAT 301 ACATAAACGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 361 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 421 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 481 TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC 541 TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT 601 GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT 661 CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA GGCCAGGGGG 721 AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC 781 AGTTAATCCT GGCCTGTTAG AAACATCAGA AGCTGTAGAC AAATACTGGG ACAGCTACAA 841 CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT ATAATACAGT AGCAACCCTC 901 TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG AAGCTTTAGA CAAGATAGAG 961 GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG GCCGCTGATC TTCAGACCCT 1021 GGGAGAAGGA GATATGGAGG GAACATTTGG GAGAAAGTGA ATTATATAAA TATAAGGTAG 1081 TGAAAATTTG AAACCATAGG GAGTAGCACC CCAGCCAGGC AAAGAGAAGA GTTGTGCAGA 1141 AAGCAAAAAG AGCATGGGGA TTAGGGAGCT TTTGTTTTCG CTCGTG //
Primer: HIVgp_R1 () Sequence file: RDB13379_A5E8f.seq
>13379_13379_A9D8F_3_HIVgp_R1_D08_11_ABI24.ab1 1 CCGGCCTCCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG 61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA 121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGCTTGCT GTGCGGTGGT 181 CTTACTTTTG TTTTGCTCTT CCTCTATCTT GTCTAAAGCT TCCTTGGTGT CTTTTATCTC 241 TATCCTTTGA TGCACACAAT AGAGGGTTGC TACTGTATTA TATAATGATC TAAGTTCTTC 301 TGATCCTGTC TGAAGGGATG GTTGTAGCTG TCCCAGTATT TGTCTACAGC CTTCTGATGT 361 TTCTAACAGG CCAGGATTAA CTGCGAATCG TTCTAGCTCC CTGCTTGCCC ATACTATATG 421 TTTTAATTTA TATTTTTTCT TTCCCCCTGG CCTTAACCGA ATTTTTTCCC ATCGCGATCT 481 AATTCTCCCC CGCTTAATAC TGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT 541 AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC 601 AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT TCAAGTCCCT 661 GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG 721 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT 781 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT 841 AACCAGAGAG ACCCGTTTAT GTATCGAGCT AGGCACTTAA ATACAATATC TCTGCAATGC 901 GGCAATTCAG TGGTTCGTCC AATCCATGTC AGACCCGTCT GTTGCCTTCC TAATAAAGCA 961 CGATCGTACC ACCTTACTTC CACCAATCGG CATGCACGGT GCTTTTTTCT CTCCTTGTAA 1021 GGCATGTTGC TAACTCATCG TTACCATGTT TGCAGACTAC AGAGTATGCA TAAAGACTAC 1081 ACGCGGTCAA GCTTGGCAAC TGGGCCGTTC GTTTACCACG TTCATGAACT GGAAACCTGG 1141 ACGTTACCCA ACTTAAATCG CCCTTGCCAG GCAC //
Primer: SV40pro_R () Sequence file: RDB13379_A5E8g.seq
>13379_13379_A9D8F_3_SV40pro_R_B08_05_ABI24.ab1 1 GGGGGGGCCC TTGACATAAT AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG 61 GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA TAGCTAGAGC CAGACATGAT 121 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT 181 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT 241 TCCTCTCACT CTCTGATATT CATTTCTTTG CAAGTTATAA ATACTGAATA ATAAGATGAC 301 ATGAACTACT ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT 361 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT 421 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA 481 ACCAGAGAGA CCCAGTACAA GCAAAAAGCA GATCTTATCT TCGTTGGGAG TGAATTAGCC 541 CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA 601 TTGGTCTTAA AGTCGAGTTA CTGCTCGTTC TTCAGCACGC GCTCCACGAA GCTCTTGATG 661 TACTTACCCA TTTCATCTGG AGCGTCCTCC TGGAGGAAGT GGAGGCCCTT CACCTTCACG 721 AACTCGGTGT TAGGGAACTT CTTAGCTCCC TCGACAATAG CGTTGGAAAA GAACCCAGGG 781 TCGGACTCGA TGAACAGCTT AGGCAGATCG TCGCTGGCCC GAAGGTAGGC GTTGTAGTTG 841 CGGACAATCT GGGACGACGT CGGGCTTGCC TCCCTTAACG AAGAGGGGAT CTCGCGAGGC 901 CAGGAAGAGG GGTAGGCCGT CTAACCTCGC CCCTTCTCCT TGAAATGGGC TCCAGTAGGC 961 AGCGAAACTT CCTCAGGCTC CAGTTTCCCG CATGAATCCT TGGCTTGGGC AACAGGGTCT 1021 CGACAAAGAA GATTATTTCT TCAAGCACCA TTTTCTCGGC CCTTCTCGCT CCTTGGATTC 1081 AAGGGCCCAG CTTCCCTTCC TTCGAATGTC CAGGCCAACT CCCAATTCCA GGACTTCGAT 1141 CACGATATCC CACGCAACAC ATCTTCCCAT TAGTTGCACG CGAATATGAG CCCCCTATTG 1201 GTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]

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mouse Sox2

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mouse Sox2

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