Resource data sheet
Prev.

pGF-mSox2-ONL

Lentiviral reporter plasmid of mouse Sox2 by the Orange Nano-lantern.

Catalog number RDB13379
Resource name pGF-mSox2-ONL
Clone info. Lentiviral reporter plasmid of mouse Sox2 by the Orange Nano-lantern.
Expression was confirmed by the depositor with transfection into murine ES cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Reporter gene was deleted from the original vector. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone pGreenFire1 (Plasmid)
Size of vector backbone 9.4 kb
Gene/insert name mouse Sox2 genomic DNA
Depositor Okada, Yasushi | Takai, Akira |
 
Other clones in our bank mouse Sox2 (NCBI Gene 20674) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13379 pGF-mSox2-ONL DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Nano-lantern (English text)
Featured content Nano-lantern (Japanese text)
Featured content Reguration of genes in ES cells (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13379_A5E8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: env_F RDB13379_A5E8a.seq
>13379_13379_A5E8_3_env_F_E09_15.ab1
    1 GGCGTGCAGG ATCCATTCGA TTAGTGACGG ATCTGCGACG GTATCGGTTA ACTTTTAAAA
   61 GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG
  121 ACATACAAAC TAAAGAATTA CAAAAACAAA TTACAAAATT CAAAATTTTA TCGATGAATT
  181 CTAATTAATG CAGAGACTCT AAAAGAATTT CCCGGGCTCG GGCAGCCATT GTGATGCATA
  241 TAGGATTATT CACGTGGTAA TGTAATTAAT GCAGAGACTC TAAAAGAATT TCCCGGGCTC
  301 GGGCAGCCAT TGTGATGCAT ATAGGATTAT TCACGTGGTA ATGCCAGTCC AAGCTAGGCA
  361 GGTTCCCCTC TAATTAATGC AGAGACTCTA AAAGAATTTC CCGGGCTCGG GCAGCCATTG
  421 TGATGCATAT AGGATTATTC ACGTGGTAAT GAGCACAGTC GACAGTTCTT GCGGATCCAC
  481 CATGGTGAGC GTGATCAAGC CCGAGATGAA GATGAGGTAC TACATGGACG GCTCCGTCAA
  541 TGGGCATGAG TTCACAATCG AGGGTGAGGG CACAGGCAGA CCTTACGAGG GACATCAGGA
  601 GATGACACTG CGCGTCACAA TGGCCGAGGG CGGGCCAATG CCTTTCGCCT TCGACCTGGT
  661 GTCCCACGTG TTCTGTTACG GCCACAGAGT TTTTACTAAA TATCCAGAAG AGATCCCAGA
  721 CTATTTCAAG CAGGCCTTTC CTGAGGGCCT GTCCTGGGAG AGGTCCCTGG AGTTCGAGGA
  781 CGGCGGCTCC GCCTCCGTGA GCGCCCACAT CAGCCTGAGG GGCAACACCT TCTACCACAA
  841 GTCCAAGTTC ACCGGCGTGA ACTTCCCCGC CGACGGCCCC ATCATGCAGA ACCAGAGCGT
  901 GGACTGGGAG CCCTCCACCG AGAAGATCAC CGCCAGCGAC GGCGTGCTGA AAGGGCGACG
  961 TGACCATGTA CCTGAAGCTG GAGGGCGGCG GCAACCACAA GTGCCAGATG AAGACCACCC
 1021 TACACGGCCG CCCAAGGAGA TCCCTGGAGA TGCCCGGCGA CCACTACATC GGGCCACAGG
 1081 CTGCTTGAGA AGACCGAGGG GCAACATTCA CGAGCAAGTG AAGAACGGCC GTGGACCACC
1141 TCCGGTACCA AGGGTGGTTA ACCGAA
//
Primer: ONL-M_R RDB13379_A5E8b.seq
>13379_13379_A9D8F_3_ONL-M_R_A08_02_ABI24.ab1
    1 CTTTAGTTGC CTCGGTCTTC TCACCAGCCT GTGGCCGATG TAGTGGTCGC CGGGCATCTC
   61 CAGGATCTCC TTGGCGGCCT TGTAGGTGGT CTTCATCTGG CACTTGTGGT TGCCGCCGCC
  121 CTCCAGCTTC AGGTACATGG TCACGTCGCC CTTCAGCACG CCGTCGCTGG CGGTGATCTT
  181 CTCGGTGGAG GGCTCCCAGT CCACGCTCTG GTTCTGCATG ATGGGGCCGT CGGCGGGGAA
  241 GTTCACGCCG GTGAACTTGG ACTTGTGGTA GAAGGTGTTG CCCCTCAGGC TGATGTGGGC
  301 GCTCACGGAG GCGGAGCCGC CGTCCTCGAA CTCCAGGGAC CTCTCCCAGG ACAGGCCCTC
  361 AGGAAAGGCC TGCTTGAAAT AGTCTGGGAT CTCTTCTGGA TATTTAGTAA AAACTCTGTG
  421 GCCGTAACAG AACACGTGGG ACACCAGGTC GAAGGCGAAA GGCATTGGCC CGCCCTCGGC
  481 CATTGTGACG CGCAGTGTCA TCTCCTGATG TCCCTCGTAA GGTCTGCCTG TGCCCTCACC
  541 CTCGATTGTG AACTCATGCC CATTGACGGA GCCGTCCATG TAGTACCTCA TCTTCATCTC
  601 GGGCTTGATC ACGCTCACCA TGGTGGATCC GCAAGAACTG TCGACTGTGC TCATTACCAC
  661 GTGAATAATC CTATATGCAT CACAATGGCT GCCCGAGCCC GGGAAATTCT TTTAGAGTCT
  721 CTGCATTAAT TAGAGGGGAA CCTGCCTAGC TTGGACTGGC ATTACCACGT GAATAATCCT
  781 ATATGCATCA CAATGGCTGC CCGAGCCCGG GAAATTCTTT TAGAGTCTCT GCATTAATTA
  841 CATTACCACG TGAATAATCC TATATGCAAT CACAATGGCT GCCCGAGCCC GGGAAATTTC
  901 TTTTAGAGTC TCTGCATTAA TTAGAATCAT CGATAAAATT TTTGAATTTT GTAATTTGGT
  961 TTTTGGAAAT TCTTTAGTTT GTATGTCTGG TTGCTATTAT GTCTACTATT CTTTCCCCTG
 1021 CACTGTACCC CCCAATCCCC CCCTTTTCTT TAAAAGTTAA ACCGAATACC GGTCCAAGAA
 1081 TCCGGTTTCA CTAAGTCGGA ATGGAATTCT AGATCTCTAT CTCATCATCT TCTCAACGAT
1141 CCTGTATCTA TGTCGAATTA TCCCAGTGCC GGGGCCCGTA TGATTG
//
Primer: mKO2-M_F RDB13379_A5E8c.seq
>13379_13379_A9D8F_3_mKO2-M_F_C04_03_ABI08.ab1
    1 TGGGCGGGCG GCCAACAACA AGGTGGCCAG ATGAAGACCA CCTACAAGGC CGCCAAGGAG
   61 ATCCTGGAGA TGCCCGGCGA CCACTACATC GGCCACAGGC TGGTGAGGAA GACCGAGGGC
  121 AACATCACCG AGCAGGTGGA GGACGCCGTG GCCCACTCCG GTACCAAGGT GTACGACCCC
  181 GAGCAACGCA AACGCATGAT CACTGGGCCT CAGTGGTGGG CTCGCTGCAA GCAAATGAAC
  241 GTGCTGGACT CCTTCATCAA CTACTATGAT TCCGAGAAGC ACGCCGAGAA CGCCGTGATT
  301 TTTCTGCATG GTAACGCTAC CTCCAGCTAC CTGTGGAGGC ACGTCGTGCC TCACATCGAG
  361 CCCGTGGCTA GATGCATCAT CCCTGATCTG ATCGGAATGG GTAAGTCCGG CAAGAGCGGG
  421 AATGGCTCAT ATCGCCTCCT GGATCACTAC AAGTACCTCA CCGCTTGGTT CGAGCTGCTG
  481 AACCTTCCAA AGAAAATCAT CTTTGTGGGC CACGACTGGG GGAGCGCTCT GGCCTTTCAC
  541 TACGCCTACG AGCACCAAGA CAGGATCAAG GCCATCGTCC ATATGGAGAG TGTCGTGGAC
  601 GTGATCGAGT CCTGGATGGG GTGGCCTGAC ATCGAGGAGG AGCTGGCCCT GATCAAGAGC
  661 GAAGAGGGCG AGAAAATGGT GCTTGAGAAT AACTTCTTCG TCGAGACCCT GTTGCCAAGC
  721 AAGATCATGC GGGAAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG CCATTCAAGG
  781 AAGAAGGGCG AGGTTAGACG GCCTACCCTC TCCTGGCCTC GCGAGATCCC CTCTCTTAAG
  841 GGAAGGCAAA GCCCGAACGT CGTCCAGATT GTTCCGCAAC TAACAACGGC CTACCTTCCG
  901 GCCAACCAAC GATCTTGCCT AAACTGTTTC ATCAAGTCCA ACCCCTGGGT TTCTTTTCCA
  961 CGCTTATTTG TCGAGGGGAG CTAAAAAAGT CCCTAGAGCC GAAGTTTTGT GAAGGTTGAA
 1021 CGGGGCCCCA ACTTCCTACC AGAAGGACGC TCGCAGAATA TAAATGGGGA AAGTACCACT
1081 CAG
//
Primer: RLuc8.6-M_F RDB13379_A5E8d.seq
>13379_13379_A9D8F_3_RLuc8.6-M_F_H05_08_ABI08.ab1
    1 CGTAGTGGGG GGGGGCCTGA CATCGAGGAG GAGCTGGCCC TGATCAAGAG CGAAGAGGGC
   61 GAGAAAATGG TGCTTGAGAA TAACTTCTTC GTCGAGACCC TGTTGCCAAG CAAGATCATG
  121 CGGAAACTGG AGCCTGAGGA GTTCGCTGCC TACCTGGAGC CATTCAAGGA GAAGGGCGAG
  181 GTTAGACGGC CTACCCTCTC CTGGCCTCGC GAGATCCCTC TCGTTAAGGG AGGCAAGCCC
  241 GACGTCGTCC AGATTGTCCG CAACTACAAC GCCTACCTTC GGGCCAGCGA CGATCTGCCT
  301 AAGCTGTTCA TCGAGTCCGA CCCTGGGTTC TTTTCCAACG CTATTGTCGA GGGAGCTAAG
  361 AAGTTCCCTA ACACCGAGTT CGTGAAGGTG AAGGGCCTCC ACTTCCTCCA GGAGGACGCT
  421 CCAGATGAAA TGGGTAAGTA CATCAAGAGC TTCGTGGAGC GCGTGCTGAA GAACGAGCAG
481 TAACTCGACT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATC
//
Primer: pUC-M13_100 RDB13379_A5E8e.seq
>13379_13379_A9D8F_3_pUC-M13_-100_C08_08_ABI24.ab1
    1 GCCGTCAGGC GATTAGTTGG GTACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC
   61 GGCCAGTGCC AAGCTGACGC GTGTAGTCTT ATGCAATACT CTTGTAGTCT TGCAACATGG
  121 TAACGATGAG TTAGCAACAT GCCTTACAAG GAGAGAAAAA GCACCGTGCA TGCCGATTGG
  181 TGGAAGTAAG GTGGTACGAT CGTGCCTTAT TAGGAAGGCA ACAGACGGGT CTGACATGGA
  241 TTGGACGAAC CACTGAATTG CCGCATTGCA GAGATATTGT ATTTAAGTGC CTAGCTCGAT
  301 ACATAAACGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG
  361 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC
  421 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA
  481 TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC
  541 TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT
  601 GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT
  661 CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA GGCCAGGGGG
  721 AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC
  781 AGTTAATCCT GGCCTGTTAG AAACATCAGA AGCTGTAGAC AAATACTGGG ACAGCTACAA
  841 CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT ATAATACAGT AGCAACCCTC
  901 TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG AAGCTTTAGA CAAGATAGAG
  961 GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG GCCGCTGATC TTCAGACCCT
 1021 GGGAGAAGGA GATATGGAGG GAACATTTGG GAGAAAGTGA ATTATATAAA TATAAGGTAG
 1081 TGAAAATTTG AAACCATAGG GAGTAGCACC CCAGCCAGGC AAAGAGAAGA GTTGTGCAGA
1141 AAGCAAAAAG AGCATGGGGA TTAGGGAGCT TTTGTTTTCG CTCGTG
//
Primer: HIVgp_R1 RDB13379_A5E8f.seq
>13379_13379_A9D8F_3_HIVgp_R1_D08_11_ABI24.ab1
    1 CCGGCCTCCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGCTTGCT GTGCGGTGGT
  181 CTTACTTTTG TTTTGCTCTT CCTCTATCTT GTCTAAAGCT TCCTTGGTGT CTTTTATCTC
  241 TATCCTTTGA TGCACACAAT AGAGGGTTGC TACTGTATTA TATAATGATC TAAGTTCTTC
  301 TGATCCTGTC TGAAGGGATG GTTGTAGCTG TCCCAGTATT TGTCTACAGC CTTCTGATGT
  361 TTCTAACAGG CCAGGATTAA CTGCGAATCG TTCTAGCTCC CTGCTTGCCC ATACTATATG
  421 TTTTAATTTA TATTTTTTCT TTCCCCCTGG CCTTAACCGA ATTTTTTCCC ATCGCGATCT
  481 AATTCTCCCC CGCTTAATAC TGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT
  541 AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC
  601 AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT TCAAGTCCCT
  661 GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
  721 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
  781 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
  841 AACCAGAGAG ACCCGTTTAT GTATCGAGCT AGGCACTTAA ATACAATATC TCTGCAATGC
  901 GGCAATTCAG TGGTTCGTCC AATCCATGTC AGACCCGTCT GTTGCCTTCC TAATAAAGCA
  961 CGATCGTACC ACCTTACTTC CACCAATCGG CATGCACGGT GCTTTTTTCT CTCCTTGTAA
 1021 GGCATGTTGC TAACTCATCG TTACCATGTT TGCAGACTAC AGAGTATGCA TAAAGACTAC
 1081 ACGCGGTCAA GCTTGGCAAC TGGGCCGTTC GTTTACCACG TTCATGAACT GGAAACCTGG
1141 ACGTTACCCA ACTTAAATCG CCCTTGCCAG GCAC
//
Primer: SV40pro_R RDB13379_A5E8g.seq
>13379_13379_A9D8F_3_SV40pro_R_B08_05_ABI24.ab1
    1 GGGGGGGCCC TTGACATAAT AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG
   61 GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA TAGCTAGAGC CAGACATGAT
  121 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT
  181 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT
  241 TCCTCTCACT CTCTGATATT CATTTCTTTG CAAGTTATAA ATACTGAATA ATAAGATGAC
  301 ATGAACTACT ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
  361 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  421 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  481 ACCAGAGAGA CCCAGTACAA GCAAAAAGCA GATCTTATCT TCGTTGGGAG TGAATTAGCC
  541 CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA
  601 TTGGTCTTAA AGTCGAGTTA CTGCTCGTTC TTCAGCACGC GCTCCACGAA GCTCTTGATG
  661 TACTTACCCA TTTCATCTGG AGCGTCCTCC TGGAGGAAGT GGAGGCCCTT CACCTTCACG
  721 AACTCGGTGT TAGGGAACTT CTTAGCTCCC TCGACAATAG CGTTGGAAAA GAACCCAGGG
  781 TCGGACTCGA TGAACAGCTT AGGCAGATCG TCGCTGGCCC GAAGGTAGGC GTTGTAGTTG
  841 CGGACAATCT GGGACGACGT CGGGCTTGCC TCCCTTAACG AAGAGGGGAT CTCGCGAGGC
  901 CAGGAAGAGG GGTAGGCCGT CTAACCTCGC CCCTTCTCCT TGAAATGGGC TCCAGTAGGC
  961 AGCGAAACTT CCTCAGGCTC CAGTTTCCCG CATGAATCCT TGGCTTGGGC AACAGGGTCT
 1021 CGACAAAGAA GATTATTTCT TCAAGCACCA TTTTCTCGGC CCTTCTCGCT CCTTGGATTC
 1081 AAGGGCCCAG CTTCCCTTCC TTCGAATGTC CAGGCCAACT CCCAATTCCA GGACTTCGAT
 1141 CACGATATCC CACGCAACAC ATCTTCCCAT TAGTTGCACG CGAATATGAG CCCCCTATTG
1201 GTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

2019.05.21

GNP_filter3_RDBDEP_html_190430.pl