Resource data sheet
Prev.

pRZ-mOct4-CNL

Lentiviral reporter plasmid of mouse Oct4 by the Cyan Nano-lantern.

Catalog number RDB13378
Resource name pRZ-mOct4-CNL
Clone info. Lentiviral reporter plasmid of mouse Oct4 by the Cyan Nano-lantern.
Expression was confirmed by the depositor with transfection into murine ES cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Reporter gene was deleted from the original vector. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam.
Vector backbone pRedZeo (Plasmid)
Size of vector backbone 9.7 kb
Gene/insert name mouse Oct4 genomic DNA
Depositor Okada, Yasushi | Takai, Akira |
 
Other clones in our bank mouse Pou5f1 (NCBI Gene 18999) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infectious virus.
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13378 pRZ-mOct4-CNL DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Nano-lantern (English text)
Featured content Nano-lantern (Japanese text)
Featured content Reguration of genes in ES cells (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13378_A5E8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: env_F RDB13378_A5E8a.seq
>13378_13378_A9D8F_env_F_2_A11_02_ABI24.ab1
    1 ACGGGCAGAT CCATTCGATT AGTGAACGGA TCTCGACGGT ATCGGTTAAC TTTTAAAAGA
   61 AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC
  121 ATACAAACTA AAGAATTACA AAAACAAATT ACAAAATTCA AAATTTTATC GATGGCAGAT
  181 GCATAACAAA GGCCCTGCCC CTCCCCCCAG GGAGGTTGAG AGTTCTGGGC AGACGGCAGA
  241 TGCATAACAA AGGTGCATGC CCTGCCCCTC CCCCCAGGGA GGTTGAGAGT TCTGGGCAGA
  301 CGGCAGATGC ATAACAAAGG TGCATGGGGT GTGGGGAGGT TGTAGCCCGA CCCTGCCCCT
  361 CCCCCCAGGG AGGTTGAGAG TTCTGGGCAG ACGGCAGATG CATAACAAAG GTGCATGATA
  421 GCTCTGCCCT GGGGGCAGAG AAGATGGTTG GGGAGGGGTC CCTCTCGTCC TAGTCGGATC
  481 CACCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
  541 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
  601 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
  661 GCCCACCCTC GTGACCACCC TGTCCTGGGG CGTGCAGTGC TTCGCCCGCT ACCCCGACCA
  721 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
  781 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
  841 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGAGGACGG CAACATCCTG
  901 GGGCACAAGC TGGAGTACAA CTACTTTAGC GACAACGTCT ATATCACCGC CGACAAGCAG
  961 AAGGAACGGC ATCAAGGCCC AACTTCAAGA TCCGGCCACA ACATCGAGGA CGCGGGCGTG
 1021 CAGCTCGCCG ACCACTACCA GCAGACACCC CCCAATCGGC GACGCCCCGT GCCTGCTGCC
 1081 CGAACACACT AACTTGAAGC ACCCAGTCCA GCCTGAAGCA AAGAACCCTA ACGAAGAAAG
1141 CCGCCGGAAA TCCCACACAT G
//
Primer: EGFP-C_rev RDB13378_A5E8b.seq
>13378_13378_A9D8F_3_EGFP-C_rev_E07_13_ABI24.ab1
    1 AACCGGATGG GGGTCTTTGC TCAGCTTGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
   61 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC
  121 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC
  181 GGCGGTGATA TAGACGTTGT CGCTAAAGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
  241 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
  301 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
  361 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
  421 GTAGCGGGCG AAGCACTGCA CGCCCCAGGA CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
  481 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
  541 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
  601 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG TGGATCCGAC
  661 TAGGACGAGA GGGACCCCTC CCCAACCATC TTCTCTGCCC CCAGGGCAGA GCTATCATGC
  721 ACCTTTGTTA TGCATCTGCC GTCTGCCCAG AACTCTCAAC CTCCCTGGGG GGAGGGGCAG
  781 GGGTCGGGCT ACAACCTCCC CACACCCCCA TGCACCTTTT GTTATGCATC TGCCGTCTGC
  841 CCCAGAACTC TCAACCTCCC TGGGGGGGAA GGGGCAAGGC ATGCGACCTT TGGTAATGCA
  901 TCTGCCGTCT GGCCCAGAAC TCTCAACCCT ACCTGGAGGG AGGGGCCGGG GCCTTTGGTA
  961 AGGGATCTTC CCATCGAGAA AATTTTGAAT TTGAAATTTG GTGATTGTAA TTCATAACGT
 1021 GAAAGCTGTG AGGGCAGCAA GGACCTACCA TGACTACCGA CATGCACTGT CTCCCCCCCC
1081 AATGCCCCCG CACTTGTACT TATTAAAAGA GATAAAAGAG AATACGCGAG ACT
//
Primer: mTurquoise2-M_F RDB13378_A5E8c.seq
>13378_13378_A9D8F_3_mTurquoise2-M_F_E03_05_ABI08.ab1
    1 GTCCCCAAGC ATTCGCCCGG ACCACTACCA GGCAGAACAC CCCCATCGGC GACGGCCCCG
   61 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCAA GCTGAGCAAA GACCCCAACG
  121 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGGGT ACCAAGGTGT
  181 ACGACCCCGA GCAGAGGAAG AGGATGATCA CCGGCCCCCA GTGGTGGGCC AGGTGCAAGC
  241 AGATGAACGT GCTGGACAGC TTCATCAACT ACTACGACAG CGAGAAGCAC GCCGAGAACG
  301 CCGTGATCTT CCTGCACGGC AACGCCACTA GCAGCTACCT GTGGAGGCAC GTGGTGCCCC
  361 ACATCGAGCC CGTGGCCAGG TGCATCATCC CCGATCTGAT CGGCATGGGC AAGAGCGGCA
  421 AGAGCGGCAA CGGCAGCTAC AGGCTGCTGG ACCACTACAA GTACCTGACC GCCTGGTTCG
  481 AGCTTCTGAA CCTGCCCAAG AAGATCATCT TCGTGGGCCA CGACTGGGGC GCCGCCCTGG
  541 CCTTCCACTA CGCCTACGAG CACCAGGACA GGATCAAGGC CATCGTGCAC ATGGAGAGCG
  601 TGGTGGACGT GATCGAGAGC TGGGACGAGT GGCCAGACAT CGAGGAGGAC ATCGCCCTGA
  661 TCAAGAGCGA GGAGGGCGAG AAGATGGTGC TGGAGAACAA CTTCTTCGTG GAGACCGTGC
  721 TGCCCAGCAA GATCATGAGA AAGCTGGAGC CCGAGGAGTT CGCCGCCTAC CTGGAGCCCT
  781 TCAAGGAGAA GGGCGAGGTG AGAAGACCCA CCCTGAGCTG GCCCAGAGAG ATCCCCCCTG
  841 GGTGAAGGGG CGGCAAGGCC CGGACGTGGT GCAGATCGTG AGAAACTACA AACGCCCTAC
  901 CCTGAGAGCC AGGCGACGAC CTGCCCAAGC TGTTTCATCG AGGGCGACCC CCGGGCTTCT
  961 TCAGCAACGC CATCGTGAAG GGCGCCAAAA AAGTTCCCCC AACACCGAGT TCGGTGAAGG
 1021 TGGAAGGGGC CTGCACTTCC TCCAAGAGGA CCGCCCCCCG AACGAGGATG GGCAAGTTAC
 1081 CATCAAAAAG CTTCCTGGGA AGAGAGTGCT GGAAGAAACG AAGCCAGTAA CCTGCGACCT
 1141 CGACAATCAA ACCTCTCTGG AATCACAAAA AGTTTGGTGT AAGAGAAATT GGACTCAGGG
1201 TTTATTCTCC GTTGTACCGC TA
//
Primer: PRE_rev RDB13378_A5E8d.seq
>13378_13378_A9D8F_PRE_rev_B10_04_ABI24.ab1
    1 ACGGATCCCA TAGCGTAAAA GGAGCAACAT AGTTAAGAAT ACCAGTCAAT CTTTCACAAA
   61 TTTTGTAATC CAGAGGTTGA TTGTCGAGTC GAGTTACTGC TCGTTCTTCA GCACTCTCTC
  121 CACGAAGCTC TTGATGTACT TGCCCATCTC GTCGGGGGCG TCCTCCTGGA GGAAGTGCAG
  181 GCCCTTCACC TTCACGAACT CGGTGTTGGG GAACTTCTTG GCGCCCTCCA CGATGGCGTT
  241 GCTGAAGAAG CCGGGGTCGC CCTCGATGAA CAGCTTGGGC AGGTCGTCGC TGGCTCTCAG
  301 GTAGGCGTTG TAGTTTCTCA CGATCTGCAC CACGTCGGGC TTGCCGCCCT TCACCAGGGG
  361 GATCTCTCTG GGCCAGCTCA GGGTGGGTCT TCTCACCTCG CCCTTCTCCT TGAAGGGCTC
  421 CAGGTAGGCG GCGAACTCCT CGGGCTCCAG CTTTCTCATG ATCTTGCTGG GCAGCACGGT
  481 CTCCACGAAG AAGTTGTTCT CCAGCACCAT CTTCTCGCCC TCCTCGCTCT TGATCAGGGC
  541 GATGTCCTCC TCGATGTCTG GCCACTCGTC CCAGCTCTCG ATCACGTCCA CCACGCTCTC
  601 CATGTGCACG ATGGCCTTGA TCCTGTCCTG GTGCTCGTAG GCGTAGTGGA AGGCCAGGGC
  661 GGCGCCCCAG TCGTGGCCCA CGAAGATGAT CTTCTTGGGC AGGTTCAGAA GCTCGAACCA
  721 GGCGGTCAGG TACTTGTAGT GGTCCAGCAG CCTGTAGCTG CCGTTGCCGC TCTTGCCGCT
  781 CTTGCCCATG CCGATCAGAT CGGGGATGAT GCACCTGGCC ACGGGCTCGA TGTGGGGCAC
  841 CACGTGCCTC CACAGGTAGC TGCTAGTGGC GTTGCCGTGC AGGAAGATCA CGGCGTTCTC
  901 GGCGTGCTTC TCGCTGTCGT AGTAGTTGAT GAAGCTGTCC AGCACGTTCA TCTGCTTGCA
  961 CCTGGGCCCA CCAACTGGGG GGCCGGGTGA TCAATCCTCT TTCCTCTGCT CGGGGTTCGT
 1021 ACACCTTTGG TAACCCCCCG GGCGGCGGTC ACGAAACTCC AGCAGGACCC ATGTGAATCG
1081 CCGCTTCTCG TTTGGGGTCC TTTGCTCAAC TTGGAACTGG GGTGCCTCAA
//
Primer: pUC-M13_100 RDB13378_A5E8e.seq
>13378_13378_A9D8F_3_pUC-M13_-100_G07_19_ABI24.ab1
    1 GACCGTCAGG CGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
   61 CGGCCAGTGC CAAGCTGACG CGTGTAGTCT TATGCAATAC TCTTGTAGTC TTGCAACATG
  121 GTAACGATGA GTTAGCAACA TGCCTTACAA GGAGAGAAAA AGCACCGTGC ATGCCGATTG
  181 GTGGAAGTAA GGTGGTACGA TCGTGCCTTA TTAGGAAGGC AACAGACGGG TCTGACATGG
  241 ATTGGACGAA CCACTGAATT GCCGCATTGC AGAGATATTG TATTTAAGTG CCTAGCTCGA
  301 TACATAAACG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA
  361 GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC
  421 CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA
  481 ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA GAGGAGCTCT
  541 CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG CGGCGACTGG
  601 TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG TGCGAGAGCG
  661 TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA AGGCCAGGGG
  721 GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG
  781 CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG GGGACAGCTA
  841 CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC
  901 CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAAGCTT TTAGACAAGA
  961 TAGAGGAAGA GCAAAACAAA AAGTAAGACC ACCGCACAGC AAAGCGGCCC GCTGATTCTT
 1021 CAGACCCTGG GAGGAAGGAG GATTATGAGG GACAATTTGG GAGAAGTTGA ATTAATATTA
 1081 AATTATAAAG TAGGTAAGAA ATTTGCGCTA TTAGGAGTTA GCAACCCGAC CGAGGCAAAA
 1141 GGAATAGTGT GCCATAGAGA AAAGAATGAG CCAGTTGGGG CATTGGGAGA GCCCTTTGTG
1201 TTTCCTCCGT
//
Primer: HIVgp_R1 RDB13378_A5E8f.seq
>13378_13378_A5E8_3_HIVgp_R1_B11_05.ab1
    1 CCAGGCCTCT ATTCCCACTG CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT
   61 GGGTGCTACT CCTAATGGTT CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA
  121 ATTGTCCCTC ATATCTCCTC CTCCAGGTCT GAAGATCAGC GGCCGCTTGC TGTGCGGTGG
  181 TCTTACTTTT GTTTTGCTCT TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT
  241 CTATCCTTTG ATGCACACAA TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT
  301 CTGATCCTGT CTGAAGGGAT GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG
  361 TTTCTAACAG GCCAGGATTA ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT
  421 GTTTTAATTT ATATTTTTTC TTTCCCCCTG GCCTTAACCG AATTTTTTCC CATCGCGATC
  481 TAATTCTCCC CCGCTTAATA CTGACGCTCT CGCACCCATC TCTCTCCTTC TAGCCTCCGC
  541 TAGTCAAAAT TTTTGGCGTA CTCACCAGTC GCCGCCCCTC GCCTCTTGCC GTGCGCGCTT
  601 CAGCAAGCCG AGTCCTGCGT CGAGAGAGCT CCTCTGGTTT CCCTTTCGCT TTCAAGTCCC
  661 TGTTCGGGCG CCACTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA
  721 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA
  781 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC
  841 TAACCAGAGA GACCCGTTTA TGTATCGAGC TAGGCACTTA AATACAATAT CTCTGCAATG
  901 CGGCAATTCA GTGGTTCGTC CAATCCATGT CAGACCCGTC TGTTGCCTTC CTAATAAGCA
  961 CGATCGTACC ACCTTACTTC CACCAATCGG CATGCACGGT GCTTTTTCTC TCCTTTGTAA
 1021 CGCATGTTTG CTAACTCATC GTTTACCATG TTGCAGACTA CAGAGTATTG CATAAGACTA
 1081 ACACGCGTCA GCTTTCCACT GCCCGTCGAT TTACACCGTT CGTTGACCTG AAAAACCCTG
1141 ACGTTTACCT CAAAACCTTT AAAT
//
Primer: SV40pro_R RDB13378_A5E8g.seq
>13378_13378_A9D8F_3_SV40pro_R_H07_22_ABI24.ab1
    1 GGGGGGGCCT TGGCATAATA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT
   61 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ATAGCTAGAG CCAGACATGA
  121 TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA
  181 TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG
  241 TTCCTCTCAC TCTCTGATAT TCATTTCTTT GCAAGTTATA AATACTGAAT AATAAGATGA
  301 CATGAACTAC TACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
  361 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
  421 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
  481 AACCAGAGAG ACCCAGTACA AGCAAAAAGC AGATCTTATC TTCGTTGGGA GTGAATTAGC
  541 CCTTCCAGTC CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC
  601 ATTGGTCTTA AAGGTACCGA GCTCGAATTA ATTCCAGGCG GGGAGGCGGC CCAAAGGGAG
  661 ATCCGACTCG TCTGAGGGCG AAGGCGAAGA TGCGGAAGAG GCCGCAGAGC CGGCAGCAGG
  721 CCGCGGGAAG GAAGGTCCGC TGGATTGAGG GCCGAAGGGA CGTAGCAGAA GGACGTCCCG
  781 CGCAGAATCC AGGTGGCAAC ACAGGGCGAG CAGCCCATGG GAAAGGGACG TCAGCTTCCC
  841 CGACAACACC ACGGAAATTG TCAGTGCCCA ACAGCCCGAG CCCCCTGTCC CAGCCAGCGG
  901 GCAAAGGCAG GCCGGCGATG AGTTTCCGCC CGTGGCAATA GGGGAGGGGG GAAAGCGAAA
  961 GTCCCCGGAA AAGGAGCTAA CAGGGTGGTG GGAAATTGCC CCAACCAAGT GGGGGGTTTG
 1021 CGTCAGCCAA ACACCAGTGC ACACCAACGC CATCCGTTTG CCTTGACAAT CGGGTCCCAC
 1081 AACTCCTTCA TAAGAGACAC TCCGACCAGG GATTTTTAAT ACTACGGAGA GGGAGCAAAA
 1141 TGTGAAATAG CCAAATACCC GGAAAGGCAT TTGAGCCAGG GAATACCATA GGAGAGCATC
1201 ATATAAAAGC GTCCATCGCG TAAATACGCG CCCGAACATA TGATTGACGG CGCAGT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

2019.05.21

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