Resource data sheet
Prev.

pRZ-mNanog-YNL

Lentiviral reporter plasmid of mouse Nanog by the Yellow Nano-lantern.

Catalog number RDB13377
Resource name pRZ-mNanog-YNL
Clone info. Lentiviral reporter plasmid of mouse Nanog by the Yellow Nano-lantern.
Expression was confirmed by the depositor with transfection into murine ES cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Reporter gene was deleted from the original vector. YNL was provided by Dr. Takeharu Nagai, Osaka University.
Vector backbone pRedZeo (Plasmid)
Size of vector backbone 9.7 kb
Gene/insert name mouse Nanog genomic DNA
Depositor Okada, Yasushi | Takai, Akira |
 
Other clones in our bank mouse Nanog (NCBI Gene 71950) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13377 pRZ-mNanog-YNL DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Nano-lantern (English text)
Featured content Nano-lantern (Japanese text)
Featured content Reguration of genes in ES cells (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13377_A5E8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: env_F RDB13377_A5E8a.seq
>13377_13377_A5E8_1_env_F_B06_06.ab1
    1 GCGGGCAGGA TCCATTCGAT TAGTGACGGA TCTCGACGGT ATCGGTTAAC TTTTAAAAGA
   61 AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC
  121 ATACAAACTA AAGAATTACA AAAACAAATT ACAAAATTCA AAATTTTATC GATGGTGTAA
  181 ACAGTGGGTC TGAAGCCTTC GAGGGAGGAG GGCAAGATGC CGCGCTCTGC GTTTCTCCAG
  241 CTGGATGTGG ACAGAGGTCA ACCAGCCACA TTAGTTTATG TCTAAAAGTG TCTAATTGAA
  301 ACAAGAAATG GCTGCTTTAG CCGGGTGTGG TGGCACACGC CTTTAATCCC AGCACTCAGG
  361 AGGCAGAGGC AGGCAGATTT CTGAGTTCAA GGCCAGCCTG GTCTACAGAG TGAGTTCCAG
  421 GACAGCCAGA GCTACACAGA GAAACCCTGT CTCGAAAAAC CAACCAACCA ACCAACCAAA
  481 CAAACAAACA AAAAAGGAAA TGGCTGGTTT AATTATATCA CACTGTTCAT CAGAATAAAG
  541 AGAAAAATCT CAAAAAAAAA AAAAAAAAAA AAAGAGGTTC AGTCAGGCTG GGCAATGGAG
  601 GCAGTAGTGG CCGTGGTGGT CTTGGCAGCA GTGGAGGCCT TGGTGGCAGT GGTGACAGAC
  661 AGTGATAGTG TGGCAGCAGT TGAGGCAGTG GTGGTCGTAG TGGCAGTGAT GGCATTGGTG
  721 GCAGGGTTGG TGTGGCTCAG AGGTGGCAGT GGTGGCAGTG GAGGCATTGC AGGCTTTAGT
  781 GGCAATGGTA GTGGGGCAGC AGTGGTAGCC ATGGTGGCAC AGTGGTAGCA CGGTGGTAGT
  841 GGCAGCCGTG TGGCCGCAGA GCAGTGTAAC AATGGTGGCT TGTGGCAGTG CTGACAGTGT
  901 AGTATTGTAG CATGTGCAGT GAGATGTAGT GTTGTATGTG CAGGGTGCAG CGAGCCAACC
  961 GTGGTGACAT GTGCATGTGC TTGCCATGAT GCAATGTGCA TGCAATGTGC ATGATGTTGT
1021 CATGTAACTG AACTGTGCAT GAACGTATAA TGTGC
//
Primer: EGFP-C_rev RDB13377_A5E8b.seq
>13377_13377_A5E8_1_EGFP_C_rev_F10_16.ab1
    1 AAACATCCGA TGGGGGTCTT TGCTCAGCTT GGACTGGTAG CTCAGGTAGT GGTTGTCGGG
   61 CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC
  121 GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT TCTTCTGCTT
  181 GTCGGCGGTG ATATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT
  241 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC
  301 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG
  361 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC
  421 GGGGTAGCGG GCGAAGCACT GCAGGCCGTA GCCCAGGGTG GTCACGAGGG TGGGCCAGGG
  481 CACGGGCAGC TTGCCGGTGG TGCAGATCAG CTTCAGGGTC AGCTTGCCGT AGGTGGCATC
  541 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC
  601 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG TGGTGGATCC
  661 GCAGCCTTCC CACAGAAAGA GCAAGACACC AACCAAATCA GCCTATCTGA AGGCCAACGG
  721 CTCAAGGCGA TAGATTTAAA GGGTAGGTCC TGCTCCTCCC CCCTCAAGCC TCCTACCCTA
  781 CCCACCCCCT ATTCTCCCAG GCACCCAGGC GGCACCCACG CCCCACCAGC CCTGTGAATT
  841 CACAGTTAAT CCCACCTGCA GGGTCCACCA TGGACATTGT AATGCAAAAG AAGCTGTAAA
  901 GTGACCCAGA CTGGGAGGGA GGGAAAGCTT ACGGGGCATC CTCTGATCTA AGACATCCCC
  961 CCCCCCCCAA AAGAGGCTTT ACCTCATTTC ACTTTATCAT CTTTTAACCA CGGCTGCACT
 1021 CAGACCCTGC GATCTCTAGT GGGAAGTTCA GTCAGTTGGG GATCTGAGTT CCTCTCAATC
 1081 CTTCCTACCG AACATGCACG AAGTCGACTT GCTGCCAGCT CCTGGTTAAT ACACGCATTC
1141 CTTTTCAGAG ACACTTCGAA CG
//
Primer: mVenus-M_F RDB13377_A5E8c.seq
>13377_13377_A9D8F_1_mVenus-M_F_A03_01_ABI08.ab1
    1 TGCAGGCAGA GACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG AGGACGGCGG
   61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
  121 GCCCGACAAC CACTACCTGA GCTACCAGTC CAAGCTGAGC AAAGACCCCA ACGAGAAGCG
  181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG GGTACCAAGG TGTACGACCC
  241 CGAGCAGAGG AAGAGGATGA TCACCGGCCC CCAGTGGTGG GCCAGGTGCA AGCAGATGAA
  301 CGTGCTGGAC AGCTTCATCA ACTACTACGA CAGCGAGAAG CACGCCGAGA ACGCCGTGAT
  361 CTTCCTGCAC GGCAACGCCA CTAGCAGCTA CCTGTGGAGG CACGTGGTGC CCCACATCGA
  421 GCCCGTGGCC AGGTGCATCA TCCCCGATCT GATCGGCATG GGCAAGAGCG GCAAGAGCGG
  481 CAACGGCAGC TACAGGCTGC TGGACCACTA CAAGTACCTG ACCGCCTGGT TCGAGCTTCT
  541 GAACCTGCCC AAGAAGATCA TCTTCGTGGG CCACGACTGG GGCGCCGCCC TGGCCTTCCA
  601 CTACGCCTAC GAGCACCAGG ACAGGATCAA GGCCATCGTG CACATGGAGA GCGTGGTGGA
  661 CGTGATCGAG AGCTGGGACG AGTGGCCAGA CATCGAGGAG GACATCGCCC TGATCAAGAG
  721 CGAGGAGGGC GAGAAGATGG TGCTGGAGAA CAACTTCTTC GTGGAGACCG TGCTGCCCAG
  781 CAAGATCATG AGAAAGCTGG AGCCCGAGGA GTTCGCCGCC TACCTGGAGC CCTTCAAGGA
  841 GAAGGGCGAG GTGAGAAGAC CCACCCTGAG CTGGCCCAGA GAGATCCCCC TGGTGAAGGG
  901 CGGCAAGCCC GACGTGGTGC AGATCGTGAG AAACTACAAC GCCTACCTGA GAGCCAGCGA
  961 CGACCTGCCC AAGCTGTTCA TCGAGGGCGA CCCCGGCTTC TTTCAGCAAC GCCATCGTGG
 1021 AGGGCGCCAA GAAAGTTCCC CAACACCGAG TTCGTGAGGT GACGGCCTGC ACTTTCCTTC
 1081 AAGAAGACGC CCCCGACGAG ATGGACAGTA CATCAGAGCT GATGGCAGAG AGTGCCTGAA
1141 GAACGGAGCC AGTTAAACTT CGAACCT
//
Primer: PRE_rev RDB13377_A5E8d.seq
>13377_13377_A9D8F_1_PRE_rev_A07_01_ABI24.ab1
    1 ACGATCCCAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
   61 TTGTAATCCA GAGGTTGATT GTCGAGTCGA GTTACTGCTC GTTCTTCAGC ACTCTCTCCA
  121 CGAAGCTCTT GATGTACTTG CCCATCTCGT CGGGGGCGTC CTCCTGGAGG AAGTGCAGGC
  181 CCTTCACCTT CACGAACTCG GTGTTGGGGA ACTTCTTGGC GCCCTCCACG ATGGCGTTGC
  241 TGAAGAAGCC GGGGTCGCCC TCGATGAACA GCTTGGGCAG GTCGTCGCTG GCTCTCAGGT
  301 AGGCGTTGTA GTTTCTCACG ATCTGCACCA CGTCGGGCTT GCCGCCCTTC ACCAGGGGGA
  361 TCTCTCTGGG CCAGCTCAGG GTGGGTCTTC TCACCTCGCC CTTCTCCTTG AAGGGCTCCA
  421 GGTAGGCGGC GAACTCCTCG GGCTCCAGCT TTCTCATGAT CTTGCTGGGC AGCACGGTCT
  481 CCACGAAGAA GTTGTTCTCC AGCACCATCT TCTCGCCCTC CTCGCTCTTG ATCAGGGCGA
  541 TGTCCTCCTC GATGTCTGGC CACTCGTCCC AGCTCTCGAT CACGTCCACC ACGCTCTCCA
  601 TGTGCACGAT GGCCTTGATC CTGTCCTGGT GCTCGTAGGC GTAGTGGAAG GCCAGGGCGG
  661 CGCCCCAGTC GTGGCCCACG AAGATGATCT TCTTGGGCAG GTTCAGAAGC TCGAATGAGG
  721 CGGTCAGGTA CTTGTAGTGG TCCAGCAGCC TGTAGCTGCC GTTGCCGCTC TTGCCGCTCT
  781 TGCCCATGCC GATCAGATCG GGGATGATGC ACCTGGCCAC GGGCTCGATG TGGGGCACCA
  841 CGTGCCTCCA CAGGTAGCTG CTAGTGGCGT TGCCGTGCAG GAAGATCACG GCGTTCTCGG
  901 CGTGCTTCTC GCTGTCGTAG TAGTTGATGA AGCTGTCCAG CACGTTCATC TGCTTGCACC
  961 TGGGCCCACC ACTGGGGGGC CGGTGATCAT CCTCTTCCTC TGCTCGGGTC GTACACCTTT
 1021 GGTACCCCCC GGCCGGCCGG TCACGAACTC AGGCAGGACC AATGTGATCG CCGCATCTCG
 1081 TAGGGGTCTT TGCCTCAGCT GGACCTGGTA GCTCAGGTAG TGGTTGTCGG GGCAAGCCAG
1141 ACCAACGGGG GGCCGCT
//
Primer: pUC-M13_100 RDB13377_A5E8e.seq
>13377_13377_A9D8F_1_pUC-M13_-100_B07_04_ABI24.ab1
    1 GAACGTCAGG CGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
   61 CGGCCAGTGC CAAGCTGACG CGTGTAGTCT TATGCAATAC TCTTGTAGTC TTGCAACATG
  121 GTAACGATGA GTTAGCAACA TGCCTTACAA GGAGAGAAAA AGCACCGTGC ATGCCGATTG
  181 GTGGAAGTAA GGTGGTACGA TCGTGCCTTA TTAGGAAGGC AACAGACGGG TCTGACATGG
  241 ATTGGACGAA CCACTGAATT GCCGCATTGC AGAGATATTG TATTTAAGTG CCTAGCTCGA
  301 TACATAAACG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA
  361 GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC
  421 CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA
  481 ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA GAGGAGCTCT
  541 CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG CGGCGACTGG
  601 TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG TGCGAGAGCG
  661 TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA AGGCCAGGGG
  721 GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG
  781 CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG GGACAGCTAC
  841 AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA GTAGCAACCC
  901 TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA AGGAAGCTTT TAGACAAGAT
  961 AGAGGGAAGA GCAAAACAAA AGTAAGACCA CCGGCACAGC AGCGGGCCGG CTGATCTTCA
 1021 GAACCTGGGA GGAGGAGATA TGAGGGACCA TTTGGAAGAA AGTGAATTAT AATAATTATA
 1081 AAGTAGTAAA AAATTGCACC ATTTAGGAGG TAGCACCCAC TACGCCAGAC AAGAAGTTGT
1141 GCCAGAAGAG AAACAGGAGC CAGTTTGGAA TTAGGGCCTT TTTTGTGTTT CTCACG
//
Primer: HIVgp_R1 RDB13377_A5E8f.seq
>13377_13377_A5E8_1_HIVgp_R1_D06_12.ab1
    1 CAGGCCATCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGCTTGCT GTGCGGTGGT
  181 CTTACTTTTG TTTTGCTCTT CCTCTATCTT GTCTAAAGCT TCCTTGGTGT CTTTTATCTC
  241 TATCCTTTGA TGCACACAAT AGAGGGTTGC TACTGTATTA TATAATGATC TAAGTTCTTC
  301 TGATCCTGTC TGAAGGGATG GTTGTAGCTG TCCCAGTATT TGTCTACAGC CTTCTGATGT
  361 TTCTAACAGG CCAGGATTAA CTGCGAATCG TTCTAGCTCC CTGCTTGCCC ATACTATATG
  421 TTTTAATTTA TATTTTTTCT TTCCCCCTGG CCTTAACCGA ATTTTTTCCC ATCGCGATCT
  481 AATTCTCCCC CGCTTAATAC TGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT
  541 AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC
  601 AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT TCAAGTCCCT
  661 GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
  721 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
  781 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
  841 AACCAGAGAG ACCCGTTTAT GTATCGAGCT AGGCACTTAA ATACAATATC TCTGCAATGC
  901 GGCAATTCAG TGGTTCGTCC AATCCATGTC AGACCCGTCT GTTGCCTTCC TAATAAAGGC
  961 ACGATCGTAC CACCTTACTT CCACCAAATC GGCATGCACG GGTGCTTTTT TCTCTCCTTT
 1021 GTAAAGGCAT GTTTGCTAAC TCATCGTTTA CCATGTTTGC AGACTACAAG AGTAATTGCA
 1081 TAAGAACTAC ACGCGTCAGC TGGCACTTGT CGTTCGATTT TACCAACGGT CGATGGACTG
1141 CGAAAAACCC CTTGGGGCGT GC
//
Primer: SV40pro_R RDB13377_A5E8g.seq
>13377_13377_A9D8F_1_SV40pro_R_C07_07_ABI24.ab1
    1 GGGGGCCTTA CATATAAAAA TTAGTCAGCC ATGGGGCGAG AGAATGGGCG GAACTGGGCG
   61 GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGATAGC TAGAGCCAGA CATGATAAGA
  121 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT
  181 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTCCT
  241 CTCACTCTCT GATATTCATT TCTTTGCAAG TTATAAATAC TGAATAATAA GATGACATGA
  301 ACTACTACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC TCTAGTTACC
  361 AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG
  421 CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA
  481 GAGAGACCCA GTACAAGCAA AAAGCAGATC TTATCTTCGT TGGGAGTGAA TTAGCCCTTC
  541 CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG
  601 TCTTAAAGGT ACCGAGCTCG AATTAATTCC AGGCGGGGAG GCGGCCCAAA GGGAGATCCG
  661 ACTCGTCTGA GGGCGAAGGC GAAGATGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG
  721 GGAAGGAAGG TCCGCTGGAT TGAGGGCCGA AGGGACGTAG CAGAAGGACG TCCCGCGCAG
  781 AATCCAGGTG GCAACACAGG CGAGCAGCCA TGGAAAGGAC GTCAGCTTCC CCGACAAACA
  841 CCACGGAATT GTCAGTGCCC AACAGCCGAG CCCCTGTCCA GCAGCGGGCA AAGGCAGGCG
  901 GCGATGAGTT CCGCCGTGGC AATAGGGAGG GGGAAAGCGG AAAGTCCCGG GAAAGGAGCT
  961 GACAGGTGGT GGCAATGCCC CAACCAGTGG GGGGTTGCGG TCAGCAAACA CAGTGCACAC
 1021 CACGCCACGT TGCCCTGACA ACGGGGCACA CTTCTCATAA GAGGACAGCA CCAGGATTTA
 1081 TACAAGGGAG GAGAAAATTG GAAGCCATAC GGAGGCCATT AGGCATGGAT TACAAGGGCA
1141 ATTAAAGGCA GGCTCGGATG CAGTTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

2019.05.21

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