RDB13350 - pYNL-N1

Resource data sheet
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pYNL-N1_vinculin

Yellow Nano-lantern with the chicken vinculin.


Catalog number	RDB13350
Resource name	pYNL-N1_vinculin
Clone info.	Expression vector of the Yellow Nano-lantern with the chicken vinculin. Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. YNL was provided by Dr. Takeharu Nagai, Osaka University.
Vector backbone	pEGFP-N1 (Plasmid)
Size of vector backbone	4.7 kb
Gene/insert name	mouse vinculin cDNA
Depositor	Okada, Yasushi \| Takai, Akira \|
Other clones in our bank	mouse Vcl (NCBI Gene 22330) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution	RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供条件	利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
付加的提供条件	提供依頼者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] 宮脇敦史博士による提供承諾書 [Word]
備考	宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13350	pYNL-N1_vinculin	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content	Nano-lantern (English text)
Featured content	Nano-lantern (Japanese text)
Featured content	Organelle marker (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

2019.01.08

GNP_filter3_RDBDEP_html_181012.pl