Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pONL-N1_ITPKA

Orange Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.

Catalog number RDB13349
Resource name pONL-N1_ITPKA
Clone info. Expression vector of the Orange Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. The ITPKA portion of this plasmid was derivedfrom a plasmid made by Dr. Michael John Schell, Johns Hopkins Medical Institutions. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Gene/insert name rat inositol triphosphate 3-kinase A cDNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
Other clones in our bank

External Database
rat inositol triphosphate 3-kinase A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13349 pONL-N1_ITPKA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Nano-lantern (English text)
          Featured content Nano-lantern (Japanese text)
          Featured content Visualization of Organelles (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13349_A5E8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'(r ITPKA,hmKO)
          Sequence file: RDB13349_A5E8a.seq check
          >13349_13349_A5E8_3_CMV_Forward_D02_11.ab1
              1 NNNNNNNNNN NCNNTTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC
            121 AAGCTTATGG GCATGGCGCG ACCACGGGGC GCGGGGCCCT GCAGCCCCGG GTTGGAGCGG
            181 GCTCCGCGCC GGAGCGTCGG GGAGCTGCGC CTGCTCTTCG AAGCGCGCTG CGCCGCAGTC
            241 GCCGCCGCAG CGGCCGCAGG GTCGGATCCA CCGGTCGCCA CCATGGTGAG CGTGATCAAG
            301 CCCGAGATGA AGATGAGGTA CTACATGGAC GGCTCCGTCA ATGGGCATGA GTTCACAATC
            361 GAGGGTGAGG GCACAGGCAG ACCTTACGAG GGACATCAGG AGATGACACT GCGCGTCACA
            421 ATGGCCGAGG GCGGGCCAAT GCCTTTCGCC TTCGACCTGG TGTCCCACGT GTTCTGTTAC
            481 GGCCACAGAG TTTTTACTAA ATATCCAGAA NAGATCCCAN ACTATTTCAA GCAGGCCTTT
          541 CCTGAGGNNN NNNNNNN
          //
          Primer: Rluc8-M_F2
          Region: insert 3'(Rluc8.6-535)
          Sequence file: RDB13349_A5E8b.seq check
          >13349_13349_-B1G9F_Rluc8-M_F2_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGG GGNNCGCTCT GGCCTTTCNC TACGCCTACG AGCACCAAGA
             61 CAGGATCAAG GCCATCGTCC ATATGGAGAG TGTCGTGGAC GTGATCGAGT CCTGGATGGG
            121 GTGGCCTGAC ATCGAGGAGG AGCTGGCCCT GATCAAGAGC GAAGAGGGCG AGAAAATGGT
            181 GCTTGAGAAT AACTTCTTCG TCGAGACCCT GTTGCCAAGC AAGATCATGC GGAAACTGGA
            241 GCCTGAGGAG TTCGCTGCCT ACCTGGAGCC ATTCAAGGAG AAGGGCGAGG TTAGACGGCC
            301 TACCCTCTCC TGGCCTCGCG AGATCCCTCT CGTTAAGGGA GGCAAGCCCG ACGTCGTCCA
            361 GATTGTCCGC AACTACAACG CCTACCTTCG GGCCAGCGAC GATCTGCCTA AGCTGTTCAT
            421 CGAGTCCGAC CCTGGGTTCT TTTCCAACGC TATTGTCGAG GGAGCTAAGA AGTTCCCTAA
            481 CACCGAGTTC GTGAAGGTGA AGGGCCTCCA CTTCCTCCAG GAGGACGCTC CAGATGAAAT
            541 GGGTAAGTAC ATCAAGAGCT TCGTGGAGCG CGTGCTGAAG AACGAGCAGT AGGCGGCCGC
            601 GACTCTAGAT CATAATCNGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC
            661 TCCCNCNCNT CCCCCNGNNN CNGANNATAA AATGNATGNN ATTGNTGNTG NNNNCTNGNT
          721 NATNNNNNCT NNNNNNNGNN NNNNNNNN
          //
          Primer: hRluc_N_rev
          Region: insert mid(Rluc8.6-535,hmKO)
          Sequence file: RDB13349_A5E8c.seq check
          >13349_13349_B1GfF_3_hRluc_N_rev_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNTTNGCT TGNNGCGAGC CCNCCACTGA GGCCCAGTGA TCATGCGTTT
             61 GCGTTGCTCG GGGTCGTACA CCTTGGTACC GGAGTGGGCC ACGGCGTCCT CCACCTGCTC
            121 GGTGATGTTG CCCTCGGTCT TCCTCACCAG CCTGTGGCCG ATGTAGTGGT CGCCGGGCAT
            181 CTCCAGGATC TCCTTGGCGG CCTTGTAGGT GGTCTTCATC TGGCACTTGT GGTTGCCGCC
            241 GCCCTCCAGC TTCAGGTACA TGGTCACGTC GCCCTTCAGC ACGCCGTCGC TGGCGGTGAT
            301 CTTCTCGGTG GAGGGCTCCC AGTCCACGCT CTGGTTCTGC ATGATGGGGC CGTCGGCGGG
            361 GAAGTTCACG CCGGTGAACT TGGACTTGTG GTAGAAGGTG TTGCCCCTCA GGCTGATGTG
            421 GGCGCTCACG GAGGCGGAGC CGCCGTCCTC GAACTCCAGG GACCTCTCCC AGGACAGGCC
            481 CTCAGGAAAG GCCTGCTTGA AATAGTCTGG GATCTCTTCT GGATATTTAG TAAAAACTCT
            541 GTGGCCGTAA CAGAACACGT GGGANACCAG GTCGAANGCG AAAGGCATTG GCCCGCCCTC
            601 NGCCNTTGTG ACGCGCAGTG TCNTCTCCTG ATGTNCCTCN TAANGNCNGN CTGTGCCNTC
            661 NCCCTCNANT GNGAACNCNT GCCCATTGAN NNNNNTCNNT GNNNNANNTN NTCNTNNTCT
          721 NNGGCNNGNT NNCNCTNNNN NNGNNNNNNN NNN
          //
          Primer: mKO2-M_F
          Region: insert mid(hmKO,Rluc8.6-535)
          Sequence file: RDB13349_A5E8d.seq check
          >13349_13349_B1GfF_3_mKO2-M_F_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNGCNNGANG AAGANCACCT ACNAGGCCGC CAAGGAGATC
             61 CTGGAGATGC CCGGCGACCA CTACATCGGC CACAGGCTGG TGAGGAAGAC CGAGGGCAAC
            121 ATCACCGAGC AGGTGGAGGA CGCCGTGGCC CACTCCGGTA CCAAGGTGTA CGACCCCGAG
            181 CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG
            241 CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT
            301 CTGCATGGTA ACGCTACCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC
            361 GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT
            421 GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC
            481 CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGA GCGCTCTGGC CTTTCACTAC
            541 GCCTACGAGC ACCAAGACAG GATCAAGGCC ATCGTCCATA TGGAGAGTGT CGTGGANGTG
            601 ATCGANTCCT GNATGGGGTG GCCTGACNTC GANGANNANC TGGNCCNGAT CAAGAGCGAA
          661 NANGGNGANA AAATGGTGCT TGANAANNAC TNCNNCNNCN ANANCNNNNN NNNNN
          //
          Primer: RLuc8-m1dN3-C_F
          Region: insert 3'(Rluc8.6-535),SV40 pA,f1 ori
          Sequence file: RDB13349_A5E8e.seq check
          >13349_13349_B1GfF_3_Rluc8-m1dN3-c_F_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNCTT CGTGGANCGC GTGCTGAAGA ACGAGCAGTA GGCGGCCGCG
             61 ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT
            121 CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT
            181 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC
            241 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAGGCGT
            301 AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT
            361 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA
            421 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA
            481 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT
            541 AATCAAGTTT TTTGGGGTCG AGGTGNCGTA AAGCACNAAA TCGGAACCCT AAAGGGAGCC
            601 CCCGATTTAN AGCTTGACGG NGAAAGCCNG CGANNGTGNC GANAAANGAA NGGAANAAAG
            661 NNNAAGGANC GGGCGCTNNG GCNCNGGCNA GNGNANCGGT CANNCTGCNC NNANCNNCCN
          721 NNCCNNNNNN NNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40pro_ori,NeoR_KanR
          Sequence file: RDB13349_A5E8f.seq check
          >13349_13349_B1G9F_SV40pro_ori_F_H01_22_ABI24.ab1
              1 NNNNNNNNNN NNGCCGCCTC NGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT
             61 GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT
            121 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA
            181 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA
            241 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA
            301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA
            361 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT
            421 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG
            481 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA
            541 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA
            601 TCNNNNNCTC GCGCCNGCCG AACTGTTCGC NNGGCTCNNN GCGANCATNN NNNANGGCGA
            661 GGNNCTCGTC NTGNNCCANG NNNATNCNTG CTTNNCGAAN ATCATGGNNG AAANNGGNNN
          721 CNTTNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
          reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl