Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCNL-N1_ITPKA

Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13348_A5E8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,insert 5'(rat ITPKA(partial),mTurquoise2 5') Sequence file: RDB13348_A5E8g.seq
>13348_13348_B1I6F_CMV-Forward_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNTNNN NNNNNATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGAG   121 CTCAAGCTTA TGGGCATGGC GCGACCACGG GGCGCGGGGC CCTGCAGCCC CGGGTTGGAG   181 CGGGCTCCGC GCCGGAGCGT CGGGGAGCTG CGCCTGCTCT TCGAAGCGCG CTGCGCCGCA   241 GTCGCCGCCG CAGCGGCCGC AGGGTCGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC   301 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   361 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   421 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   481 TCCTGGGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   541 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   601 AACTACAAGA CCCGCGCCNA GGTGAANTTC GAGGGCGACA CCCTGGTGAA CCGCATCNAG   661 CTGANNGNCA TCNACNTCNN NNNGANNGNA NATCCNGGGG NACNNGCNNG NAGTACNACN 721 ANTTNNNCNN NNNNNNNN //
Primer: Rluc8-C_F2 Region: insert 3'(Rluc8 3'),SV40 pA,f1 ori Sequence file: RDB13348_A5E8b.seq
>13348_13348_B1I6F_3_Rluc8-C_F2_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTCG NNNNNNNGAA GGGCCTGCAC TTCCTCCAGG AGGACGCCCC    61 CGACGAGATG GGCAAGTACA TCAAGAGCTT CGTGGAGAGA GTGCTGAAGA ACGAGCAGTA   121 GGCGGCCGCG ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT   181 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG   241 TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA   301 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT   361 CTTAAGGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA   421 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA   481 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC   541 GTGGACTCCN ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA   601 CCATCACCCT AATCAAGTTT TTTGGGGNCG ANGTGNCGTA AAGCACTNAA TCGGANCCTA   661 ANNNNNCCCC CNATTNNNAN NTNGNNNGGN AANCNNGNNA NNNNGNNANA ANGNNNGNAN 721 NAANNNNANG NANNNGGNNC NNNGNNNNNN NNNN //
Primer: VN_F1 Region: insert mid(mTurquiose2 3',Rluc8 5') Sequence file: RDB13348_A5E8c.seq
>13348_13348_B1I6F_3_VN_F1_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNNNCGTCT ATATNNCCGC CGACAAGCAG AAGAACGGCA    61 TCAAGGCCAA CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCGACC   121 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC   181 TGAGCACCCA GTCCAAGCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC   241 TGGAGTTCGT GACCGCCGCC GGGGGTACCA AGGTGTACGA CCCCGAGCAG AGGAAGAGGA   301 TGATCACCGG CCCCCAGTGG TGGGCCAGGT GCAAGCAGAT GAACGTGCTG GACAGCTTCA   361 TCAACTACTA CGACAGCGAG AAGCACGCCG AGAACGCCGT GATCTTCCTG CACGGCAACG   421 CCACTAGCAG CTACCTGTGG AGGCACGTGG TGCCCCACAT CGAGCCCGTG GCCAGGTGCA   481 TCATCCCCGA TCTGATCGGC ATGGGCAAGA GCGGNNNNAN CGGCAACGGC AGCTACAGGC 541 TGCTGGACCA CNNNNAGTAC CNNNNNNCCT GGTTNNNNNN NNNN //
Primer: EGFP-C Region: insert mid(Rluc8) Sequence file: RDB13348_A5E8d.seq
>13348_13348_B1I6F_3_EGFP-C_C02_08_ABI24.ab1     1 GNNNNNNNNN NNNNGANCCC NANCNGAGGA AGAGGANGAT CACCGGCCCC CAGTGGTGGG    61 CCAGGTGCAA GCAGATGAAC GTGCTGGACA GCTTCATCAA CTACTACGAC AGCGAGAAGC   121 ACGCCGAGAA CGCCGTGATC TTCCTGCACG GCAACGCCAC TAGCAGCTAC CTGTGGAGGC   181 ACGTGGTGCC CCACATCGAG CCCGTGGCCA GGTGCATCAT CCCCGATCTG ATCGGCATGG   241 GCAAGAGCGG CAAGAGCGGC AACGGCAGCT ACAGGCTGCT GGACCACTAC AAGTACCTGA   301 CCGCCTGGTT CGAGCTTCTG AACCTGCCCA AGAAGATCAT CTTCGTGGGC CACGACTGGG   361 GCGCCGCCCT GGCCTTCCAC TACGCCTACG AGCACCAGGA CAGGATCAAG GCCATCGTGC   421 ACATGGAGAG CGTGGTGGAC GTGATCGAGA GCTGGGACGA GTGGCCAGAC ATCGAGGAGG   481 ACATCGCCCT GATCAAGAGC GAGGAGGGCG AGAAGATGGT GCTGGAGAAC AACTTCTTCG   541 TGGAGACCGT GCTGCCCAGC AAGATCATGA GAAAGCTGGA GCCCGAGGAN TTCGCCGCCT   601 ACCTGGAGCC CTTCAAGGAN ANGGNCGANG TGAGAANACC CNCCCTGNNC TNGCCNNNAG   661 AGATCCCCCN GNNGAANGNN NGCAGNCCGN NNNNNNGNNN ATCNNGNNNA ANNNNNNNNN 721 N //
Primer: RLuc8-M_F Region: insert 3'(Rluc8 3') Sequence file: RDB13348_A5E8e.seq
>13348_13348_B1I6F_3_Rluc8-M_F_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CGCCNACGAG CACCAGGACA GGATCAAGGC CATCGTGCAC    61 ATGGAGAGCG TGGTGGACGT GATCGAGAGC TGGGACGAGT GGCCAGACAT CGAGGAGGAC   121 ATCGCCCTGA TCAAGAGCGA GGAGGGCGAG AAGATGGTGC TGGAGAACAA CTTCTTCGTG   181 GAGACCGTGC TGCCCAGCAA GATCATGAGA AAGCTGGAGC CCGAGGAGTT CGCCGCCTAC   241 CTGGAGCCCT TCAAGGAGAA GGGCGAGGTG AGAAGACCCA CCCTGAGCTG GCCCAGAGAG   301 ATCCCCCTGG TGAAGGGCGG CAAGCCCGAC GTGGTGCAGA TCGTGAGAAA CTACAACGCC   361 TACCTGAGAG CCAGCGACGA CCTGCCCAAG CTGTTCATCG AGGGCGACCC CGGCTTCTTC   421 AGCAACGCCA TCGTGGAGGG CGCCAAGAAG TTCCCCAACA CCGAGTTCGT GAAGGTGAAG   481 GGCCTGCACT TCCTCCAGGA GGACGCCCCC GACGAGATGG GCAAGTACAT CAAGAGCTTC   541 GTGGAGAGAG TGCTGAAGAA CGAGCAGTAG GCGGCCGCGA CTCTAGATCA TAATCAGCCA   601 TACCACATTT GTAGAGGTTT TACTTGCTTT NAAAAACCTC CCACNCCTCC CCCTGAACCT   661 GNNNNTAAAA NGANNNNNAN NGTNGNTGNT NACTNGNTNA TNGNNNCNTN NNNGGNNANA 721 ANTAAAGCAN ANNNTCNCNA NNTTNNNNNA NNAANNNNNT TTTNNNNNNN NNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB13348_A5E8f.seq
>13348_13348_B1I6F_3_SV40pro_ori_F_E02_14_ABI24.ab1     1 NNNNNNNGNN GNNGCCGCCT CNGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT    61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA   121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT   181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC   241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG   301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG   361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC   421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC   481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG   541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC   601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAN GGCGAGCATG CCCGACNGCN   661 AGGNTCTCGT CNTGNNCCAN GGCNANGCCN GNTTGNCGAA NANCNTGGNG GNAAANGNNN 721 NNTTTNNNGN NTCNNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl