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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCNL-N1_ITPKA

Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.

Catalog number RDB13348
Resource name pCNL-N1_ITPKA
Clone info. Expression vector of the Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. The ITPKA portion of this plasmid was derivedfrom a plasmid made by Dr. Michael John Schell, Johns Hopkins Medical Institutions. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Gene/insert name rat inositol triphosphate 3-kinase A cDNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
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External Database
rat inositol triphosphate 3-kinase A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13348 pCNL-N1_ITPKA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Nano-lantern (English text)
          Featured content Nano-lantern (Japanese text)
          Featured content Visualization of Organelles (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13348_A5E8p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'(rat ITPKA(partial),mTurquoise2 5')
          Sequence file: RDB13348_A5E8g.seq check
          >13348_13348_B1I6F_CMV-Forward_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNTNNN NNNNNATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGAG
            121 CTCAAGCTTA TGGGCATGGC GCGACCACGG GGCGCGGGGC CCTGCAGCCC CGGGTTGGAG
            181 CGGGCTCCGC GCCGGAGCGT CGGGGAGCTG CGCCTGCTCT TCGAAGCGCG CTGCGCCGCA
            241 GTCGCCGCCG CAGCGGCCGC AGGGTCGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC
            301 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
            361 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
            421 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
            481 TCCTGGGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
            541 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
            601 AACTACAAGA CCCGCGCCNA GGTGAANTTC GAGGGCGACA CCCTGGTGAA CCGCATCNAG
            661 CTGANNGNCA TCNACNTCNN NNNGANNGNA NATCCNGGGG NACNNGCNNG NAGTACNACN
          721 ANTTNNNCNN NNNNNNNN
          //
          Primer: Rluc8-C_F2
          Region: insert 3'(Rluc8 3'),SV40 pA,f1 ori
          Sequence file: RDB13348_A5E8b.seq check
          >13348_13348_B1I6F_3_Rluc8-C_F2_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTTCG NNNNNNNGAA GGGCCTGCAC TTCCTCCAGG AGGACGCCCC
             61 CGACGAGATG GGCAAGTACA TCAAGAGCTT CGTGGAGAGA GTGCTGAAGA ACGAGCAGTA
            121 GGCGGCCGCG ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT
            181 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG
            241 TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA
            301 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT
            361 CTTAAGGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
            421 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
            481 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
            541 GTGGACTCCN ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
            601 CCATCACCCT AATCAAGTTT TTTGGGGNCG ANGTGNCGTA AAGCACTNAA TCGGANCCTA
            661 ANNNNNCCCC CNATTNNNAN NTNGNNNGGN AANCNNGNNA NNNNGNNANA ANGNNNGNAN
          721 NAANNNNANG NANNNGGNNC NNNGNNNNNN NNNN
          //
          Primer: VN_F1
          Region: insert mid(mTurquiose2 3',Rluc8 5')
          Sequence file: RDB13348_A5E8c.seq check
          >13348_13348_B1I6F_3_VN_F1_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNNNNCGTCT ATATNNCCGC CGACAAGCAG AAGAACGGCA
             61 TCAAGGCCAA CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCGACC
            121 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
            181 TGAGCACCCA GTCCAAGCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
            241 TGGAGTTCGT GACCGCCGCC GGGGGTACCA AGGTGTACGA CCCCGAGCAG AGGAAGAGGA
            301 TGATCACCGG CCCCCAGTGG TGGGCCAGGT GCAAGCAGAT GAACGTGCTG GACAGCTTCA
            361 TCAACTACTA CGACAGCGAG AAGCACGCCG AGAACGCCGT GATCTTCCTG CACGGCAACG
            421 CCACTAGCAG CTACCTGTGG AGGCACGTGG TGCCCCACAT CGAGCCCGTG GCCAGGTGCA
            481 TCATCCCCGA TCTGATCGGC ATGGGCAAGA GCGGNNNNAN CGGCAACGGC AGCTACAGGC
          541 TGCTGGACCA CNNNNAGTAC CNNNNNNCCT GGTTNNNNNN NNNN
          //
          Primer: EGFP-C
          Region: insert mid(Rluc8)
          Sequence file: RDB13348_A5E8d.seq check
          >13348_13348_B1I6F_3_EGFP-C_C02_08_ABI24.ab1
              1 GNNNNNNNNN NNNNGANCCC NANCNGAGGA AGAGGANGAT CACCGGCCCC CAGTGGTGGG
             61 CCAGGTGCAA GCAGATGAAC GTGCTGGACA GCTTCATCAA CTACTACGAC AGCGAGAAGC
            121 ACGCCGAGAA CGCCGTGATC TTCCTGCACG GCAACGCCAC TAGCAGCTAC CTGTGGAGGC
            181 ACGTGGTGCC CCACATCGAG CCCGTGGCCA GGTGCATCAT CCCCGATCTG ATCGGCATGG
            241 GCAAGAGCGG CAAGAGCGGC AACGGCAGCT ACAGGCTGCT GGACCACTAC AAGTACCTGA
            301 CCGCCTGGTT CGAGCTTCTG AACCTGCCCA AGAAGATCAT CTTCGTGGGC CACGACTGGG
            361 GCGCCGCCCT GGCCTTCCAC TACGCCTACG AGCACCAGGA CAGGATCAAG GCCATCGTGC
            421 ACATGGAGAG CGTGGTGGAC GTGATCGAGA GCTGGGACGA GTGGCCAGAC ATCGAGGAGG
            481 ACATCGCCCT GATCAAGAGC GAGGAGGGCG AGAAGATGGT GCTGGAGAAC AACTTCTTCG
            541 TGGAGACCGT GCTGCCCAGC AAGATCATGA GAAAGCTGGA GCCCGAGGAN TTCGCCGCCT
            601 ACCTGGAGCC CTTCAAGGAN ANGGNCGANG TGAGAANACC CNCCCTGNNC TNGCCNNNAG
            661 AGATCCCCCN GNNGAANGNN NGCAGNCCGN NNNNNNGNNN ATCNNGNNNA ANNNNNNNNN
          721 N
          //
          Primer: RLuc8-M_F
          Region: insert 3'(Rluc8 3')
          Sequence file: RDB13348_A5E8e.seq check
          >13348_13348_B1I6F_3_Rluc8-M_F_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN CGCCNACGAG CACCAGGACA GGATCAAGGC CATCGTGCAC
             61 ATGGAGAGCG TGGTGGACGT GATCGAGAGC TGGGACGAGT GGCCAGACAT CGAGGAGGAC
            121 ATCGCCCTGA TCAAGAGCGA GGAGGGCGAG AAGATGGTGC TGGAGAACAA CTTCTTCGTG
            181 GAGACCGTGC TGCCCAGCAA GATCATGAGA AAGCTGGAGC CCGAGGAGTT CGCCGCCTAC
            241 CTGGAGCCCT TCAAGGAGAA GGGCGAGGTG AGAAGACCCA CCCTGAGCTG GCCCAGAGAG
            301 ATCCCCCTGG TGAAGGGCGG CAAGCCCGAC GTGGTGCAGA TCGTGAGAAA CTACAACGCC
            361 TACCTGAGAG CCAGCGACGA CCTGCCCAAG CTGTTCATCG AGGGCGACCC CGGCTTCTTC
            421 AGCAACGCCA TCGTGGAGGG CGCCAAGAAG TTCCCCAACA CCGAGTTCGT GAAGGTGAAG
            481 GGCCTGCACT TCCTCCAGGA GGACGCCCCC GACGAGATGG GCAAGTACAT CAAGAGCTTC
            541 GTGGAGAGAG TGCTGAAGAA CGAGCAGTAG GCGGCCGCGA CTCTAGATCA TAATCAGCCA
            601 TACCACATTT GTAGAGGTTT TACTTGCTTT NAAAAACCTC CCACNCCTCC CCCTGAACCT
            661 GNNNNTAAAA NGANNNNNAN NGTNGNTGNT NACTNGNTNA TNGNNNCNTN NNNGGNNANA
          721 ANTAAAGCAN ANNNTCNCNA NNTTNNNNNA NNAANNNNNT TTTNNNNNNN NNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR_KanR
          Sequence file: RDB13348_A5E8f.seq check
          >13348_13348_B1I6F_3_SV40pro_ori_F_E02_14_ABI24.ab1
              1 NNNNNNNGNN GNNGCCGCCT CNGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
             61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
            121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
            181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
            241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
            301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
            361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
            421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
            481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
            541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC
            601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAN GGCGAGCATG CCCGACNGCN
            661 AGGNTCTCGT CNTGNNCCAN GGCNANGCCN GNTTGNCGAA NANCNTGGNG GNAAANGNNN
          721 NNTTTNNNGN NTCNNCNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
          reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl