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pCNL-N1_ITPKA

Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.

Catalog number RDB13348
Resource name pCNL-N1_ITPKA
Clone info. Expression vector of the Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. The ITPKA portion of this plasmid was derivedfrom a plasmid made by Dr. Michael John Schell, Johns Hopkins Medical Institutions. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Gene/insert name rat inositol triphosphate 3-kinase A cDNA
Depositor Okada, Yasushi | Takai, Akira |
 
Other clones in our bank rat Itpka (KEGG orthology K00911) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13348 pCNL-N1_ITPKA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Nano-lantern (English text)
Featured content Nano-lantern (Japanese text)
Featured content Organelle marker (English text)

References

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

2018.12.11

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