Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
Catalog number | RDB13348 |
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Resource name | pCNL-N1_ITPKA |
Clone info. | Expression vector of the Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A. Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. The ITPKA portion of this plasmid was derivedfrom a plasmid made by Dr. Michael John Schell, Johns Hopkins Medical Institutions. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam. |
Vector backbone | pEGFP-N1 (Plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli) |
Gene/insert name | rat inositol triphosphate 3-kinase A cDNA |
Depositor|Developer | Okada, Yasushi | Takai, Akira | |
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Other clones in our bank | rat Itpka (KEGG orthology K00911) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Schedule A (GFP-A) [Link] |
Remarks | Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB13348 | pCNL-N1_ITPKA | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
original | Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Nano-lantern (English text) |
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Featured content | Nano-lantern (Japanese text) |
Featured content | Visualization of Organelles (English text) |
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
2021.03.26
GNP_filter3_RDBDEP_html_210308.pl