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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pYNL-N1_TUBB5

Yellow Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.

Catalog number RDB13338
Resource name pYNL-N1_TUBB5
Clone info. Expression vector of the Yellow Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. YNL was provided by Dr. Takeharu Nagai, Osaka University.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Gene/insert name mouse beta-tubulin class V (TUBB5) cDNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
Other clones in our bank

External Database
mouse beta-tubulin class V (TUBB5)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
          Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
          付加的提供条件 利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13338 pYNL-N1_TUBB5 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Nano-lantern (English text)
          Featured content Nano-lantern (Japanese text)
          Featured content Visualization of Organelles (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13338_A5E8p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'(mouse Tubb5)
          Sequence file: RDB13338_A5E8a.seq check
          >13338_13338_B1G9F_3_CMV_Forward_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT
            121 CAAGCTTCGA ATTCCACCAT GAGGGAAATC GTGCACATCC AGGCCGGACA GTGTGGCAAC
            181 CAGATCGGTG CTAAGTTCTG GGAGGTGATA AGCGATGAAC ATGGCATCGA CCCCACCGGT
            241 ACCTACCACG GTGACAGCGA CCTGCAGCTG GACCGAATCT CTGTGTACTA TAATGAAGCC
            301 ACAGGTGGCA AGTATGTCCC TCGAGCTATC TTGGTGGATC TAGAACCTGG GACTATGGAC
          361 TCCGTTCGCT CAGGTCCTTT TGGNCNNATC TTCNNANNAG ACNNCTTCNN NNNNNNNN
          //
          Primer: Rluc8-C_F2
          Region: insert 3'(Rluc8-m1 dN3),SV40 pA,f1 ori
          Sequence file: RDB13338_A5E8b.seq check
          >13338_13338_B1G9F_3_Rluc8-C_F2_D02_11_ABI24.ab1
              1 NNNNNNNNGN NNGNNGAAGG GCCTGCACTT CCTCCNGGAG GACGCCCCCG ACGAGATGGG
             61 CAAGTACATC AAGAGCTTCG TGGAGAGAGT GCTGAAGAAC GAGCAGTAGG CGGCCGCGAC
            121 TCTAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
            181 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA
            241 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT
            301 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAAGGCGTAA
            361 ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT
            421 TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA
            481 GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC
            541 GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA
            601 TCNAGTTTTT TGGGGNCNAN GTGCCGTAAA GCACTAAATC GGAANCCTAA AGGGANCCCC
            661 CGANTTNNAN CTNGNNGGGN NAANNNNNNN ANNNNNNNAN NANNNNNNNN NANNNNNANN
          721 NNNNNGNNNN NNNNNN
          //
          Primer: Venus-N_R
          Region: insert mid(mVenus dC10,mouse Tubb5)
          Sequence file: RDB13338_A5E8c.seq check
          >13338_13338_B1G9F_3_Venus-N_R_A02_02_ABI24.ab1
              1 NNNNNNNCNN NNNTTNTGGC CGTTNNCGTC GCCGTCNNGC TCGACNNGGA TGGGCACCAC
             61 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG ACCGGTGGAT CCCGGGCCCG
            121 CGGTACCGTC GACTGGGCCT CCTCTTCTGC CTCCTCTCCG AAATCCTCTT CCTCTTCCGC
            181 GGTGGCATCC TGGTACTGCT GGTACTCAGA GACGAGGTCG TTCATGTTGC TCTCAGCTTC
            241 GGTGAACTCC ATCTCGTCCA TGCCCTCACC CGTATACCAG TGGAGGAAAG CCTTCCGGCG
            301 GAACATAGCC GTAAACTGCT CAGAGATGCG CTTGAAGAGC TCCTGGATGG CTGTGCTGTT
            361 TCCAATGAAG GTGACTGCCA TCTTGAGGCC ACGCGGTGGG ATGTCACAGA CAGCTGTCTT
            421 GACATTGTTG GGGATCCATT CCACNAAGTA GCTGCTATTC TTGTTCTGCA CGTTGAGCAT
          481 CTGCTCATCC ACCTCCTTCA TGGACATCCG TCCACGGAAG ANNNNNNNNN N
          //
          Primer: Rluc8-H_R
          Region: insert mid(Rluc8-m1 dN3,mVenus dC10)
          Sequence file: RDB13338_A5E8d.seq check
          >13338_13338_B1G9F_3_Rluc8-H_R_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTCCTCTTCC TCTGCTCGGG GTCGTACACC TTGGTACCCC
             61 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC
            121 TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA
            181 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCTCG ATGTTGTGGC
            241 GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA TAGACGTTGT
            301 GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA
            361 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG
            421 TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA
            481 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGGCG AAGCACTGCA
            541 GGCCGTAGCC CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC
            601 AGATCAGCTT CAGGGTCNGC TTGCCGTANG TGNNATCGCC CTCGCCCTCG CCGGANACGC
            661 TGAACTTGTG GNCNNTNACG TCNCNGTCNN NCTCNANCNN GANGGNNANN NNCCNNNNNA
          721 NNGCNNNTNN CCNNNNNNNN NN
          //
          Primer: tube-B_F
          Region: insert mid(mouse Tubb5,mVenus dC10)
          Sequence file: RDB13338_A5E8e.seq check
          >13338_13338_B1G9F_3_tube-B_F_A02_02_ABI24.ab1
              1 NNNNNNNGNN NNNNNNNGAG GNNTTCGGAG AGGAGGCAGA AGAGGAGGCC CAGTCGACGG
             61 TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA
            121 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
            181 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA
            241 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC
            301 AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC
            361 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC
            421 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG
            481 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA
            541 ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCNAACTTC AAGATCCGCC
            601 ACAACATCGA GGANGGCGGC GTGCAGCTCG CCGANCACTA CCAGCAGAAC ACCCCCATCG
            661 GCGANNGCCC CNNGCTGCTG CCCGANNNCC NCTANNNGAN CTNNNNGTCC NNGCNNANNN
          721 ANNNNCCNNN NNNNNNN
          //
          Primer: Rluc8-B_R
          Region: insert mid(Rluc8-m1 dN3,mVenus dC10)
          Sequence file: RDB13338_A5E8f.seq check
          >13338_13338_B1G9F_3_Rluc8-B_R_C02_08_ABI24.ab1
              1 NNNNNNNNNN NCTTTCTNNT GATCTTGCTG GGCAGCACGG TCTCCACGAA GAAGTTGTTC
             61 TCCAGCACCA TCTTCTCGCC CTCCTCGCTC TTGATCAGGG CGATGTCCTC CTCGATGTCT
            121 GGCCACTCGT CCCAGCTCTC GATCACGTCC ACCACGCTCT CCATGTGCAC GATGGCCTTG
            181 ATCCTGTCCT GGTGCTCGTA GGCGTAGTGG AAGGCCAGGG CGGCGCCCCA GTCGTGGCCC
            241 ACGAAGATGA TCTTCTTGGG CAGGTTCAGA AGCTCGAACC AGGCGGTCAG GTACTTGTAG
            301 TGGTCCAGCA GCCTGTAGCT GCCGTTGCCG CTCTTGCCGC TCTTGCCCAT GCCGATCAGA
            361 TCGGGGATGA TGCACCTGGC CACGGGCTCG ATGTGGGGCA CCACGTGCCT CCACAGGTAG
            421 CTGCTAGTGG CGTTGCCGTG CAGGAAGATC ACGGCGTTCT CGGCGTGCTT CTCGCTGTCG
            481 TAGTAGTTGA TGAAGCTGTC CAGCACGTTC ATCTGCTTGC ACCTGGCCCA CCACTGGGGG
            541 CCGGTGATCA TCCTCTTCCT CTGCTCGGGG TCGTACACCT TGGTACCCCC GGCGGCGGTC
            601 ACGAACTCCA GCANGANCNT GTGATCGCGC TTCTCGTTGG GGTCNTTGCT CANCTTGGAN
            661 TGNTANCTCN NNNANNNNNN GNCNGGCANC ANCACNGGGN NNNCNNNNAN GGGGGNNNNC
          721 NGNNGGNNNN NNNNNN
          //
          Primer: Rluc8-M_F
          Region: insert mid(Rluc8-m1 dN3)
          Sequence file: RDB13338_A5E8g.seq check
          >13338_13338_B1G9F_3_Rluc8-M_F_20_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCNNNGAC NGGNTCAAGG CCATCGTGCA
             61 CATGGAGAGC GTGGTGGACG TGATCGAGAG CTGGGACGAG TGGCCAGACA TCGAGGAGGA
            121 CATCGCCCTG ATCAAGAGCG AGGAGGGCGA GAAGATGGTG CTGGAGAACA ACTTCTTCGT
            181 GGAGACCGTG CTGCCCAGCA AGATCATGAG AAAGCTGGAG CCCGAGGAGT TCGCCGCCTA
            241 CCTGGAGCCC TTCAAGGAGA AGGGCGAGGT GAGAAGACCC ACCCTGAGCT GGCCCAGAGA
            301 GATCCCCCTG GTGAAGGGCG GCAAGCCCGA CGTGGTGCAG ATCGTGAGAA ACTACAACGC
            361 CTACCTGAGA GCCAGCGACG ACCTGCCCAA GCTGTTCATC GAGGGCGACC CCGGCTTCTT
            421 CAGCAACGCC ATCGTGGAGG GCGCCAAGAA GTTCCCCAAC ACCGAGTTCG TGAAGGTGAA
            481 GGGCCTGCAC TTCCTCCAGG AGGACGCCCC CGACGAGATG GGCAAGTACN TNNAGAGCTT
            541 CGTGGAGAGA GTGCTGAANA NNNNGCANTA NGCGGCCGCG ACTCNANNTN NTAATCAGCC
            601 NTACNACATT TGTANAGGNT TTNCTTGCTT TAAAAANCCT CCCNCACCTC CCCCTGAACC
            661 NGAANNNNAA ATGNNTGCAN NNGNTGNTNG NNNACTNNNT ATNGNNNNNT TANAANGGNN
          721 TNNNNNNNNA NNNNNANCNN N
          //
          Primer: SV40pro_ori_F
          Region: SV 40pro_ori,NeoR_KanR
          Sequence file: RDB13338_A5E8h.seq check
          >13338_13338_B1G9F_3_SV40pro_ori_F_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNGCCGC CTCNGCCTCT GANCTATTCC NGAAGTAGTG AGGAGGCTTT
             61 TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT
            121 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG
            181 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC
            241 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA
            301 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT
            361 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA
            421 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG
            481 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT
            541 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA
            601 GCATCAGGGG CTCGCGCCNG CCGAACTGTT CGCCNGGCTC NAGGCNANCA TGCCCGACGG
            661 CGANGNNCTC GNCGTGANCC NNGNNNANGN CNGCNNGNNN ANNNCNNNGN NNNAANNGNN
          721 NNCNTTNCNN NNNNCNTCNN CNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
          reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl