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pYNL-N1_TUBB5 (#RDB13338)

Yellow Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.

Clone info. Expression vector of the Yellow Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. YNL was provided by Dr. Takeharu Nagai, Osaka University.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Growth conditions LB + Kan, 37C
Gene/insert name mouse beta-tubulin class V (TUBB5) cDNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13338 pYNL-N1_TUBB5 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYNL-N1_TUBB5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13338).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13338_A5E8p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(mouse Tubb5)
Sequence file: RDB13338_A5E8a.seq check
>13338_13338_B1G9F_3_CMV_Forward_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT
  121 CAAGCTTCGA ATTCCACCAT GAGGGAAATC GTGCACATCC AGGCCGGACA GTGTGGCAAC
  181 CAGATCGGTG CTAAGTTCTG GGAGGTGATA AGCGATGAAC ATGGCATCGA CCCCACCGGT
  241 ACCTACCACG GTGACAGCGA CCTGCAGCTG GACCGAATCT CTGTGTACTA TAATGAAGCC
  301 ACAGGTGGCA AGTATGTCCC TCGAGCTATC TTGGTGGATC TAGAACCTGG GACTATGGAC
361 TCCGTTCGCT CAGGTCCTTT TGGNCNNATC TTCNNANNAG ACNNCTTCNN NNNNNNNN
//
Primer: Rluc8-C_F2 (Pr1028)
Region: insert 3'(Rluc8-m1 dN3),SV40 pA,f1 ori
Sequence file: RDB13338_A5E8b.seq check
>13338_13338_B1G9F_3_Rluc8-C_F2_D02_11_ABI24.ab1
    1 NNNNNNNNGN NNGNNGAAGG GCCTGCACTT CCTCCNGGAG GACGCCCCCG ACGAGATGGG
   61 CAAGTACATC AAGAGCTTCG TGGAGAGAGT GCTGAAGAAC GAGCAGTAGG CGGCCGCGAC
  121 TCTAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
  181 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA
  241 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT
  301 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAAGGCGTAA
  361 ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT
  421 TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA
  481 GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC
  541 GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA
  601 TCNAGTTTTT TGGGGNCNAN GTGCCGTAAA GCACTAAATC GGAANCCTAA AGGGANCCCC
  661 CGANTTNNAN CTNGNNGGGN NAANNNNNNN ANNNNNNNAN NANNNNNNNN NANNNNNANN
721 NNNNNGNNNN NNNNNN
//
Primer: Venus-N_R (Pr0341)
Region: insert mid(mVenus dC10,mouse Tubb5)
Sequence file: RDB13338_A5E8c.seq check
>13338_13338_B1G9F_3_Venus-N_R_A02_02_ABI24.ab1
    1 NNNNNNNCNN NNNTTNTGGC CGTTNNCGTC GCCGTCNNGC TCGACNNGGA TGGGCACCAC
   61 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG ACCGGTGGAT CCCGGGCCCG
  121 CGGTACCGTC GACTGGGCCT CCTCTTCTGC CTCCTCTCCG AAATCCTCTT CCTCTTCCGC
  181 GGTGGCATCC TGGTACTGCT GGTACTCAGA GACGAGGTCG TTCATGTTGC TCTCAGCTTC
  241 GGTGAACTCC ATCTCGTCCA TGCCCTCACC CGTATACCAG TGGAGGAAAG CCTTCCGGCG
  301 GAACATAGCC GTAAACTGCT CAGAGATGCG CTTGAAGAGC TCCTGGATGG CTGTGCTGTT
  361 TCCAATGAAG GTGACTGCCA TCTTGAGGCC ACGCGGTGGG ATGTCACAGA CAGCTGTCTT
  421 GACATTGTTG GGGATCCATT CCACNAAGTA GCTGCTATTC TTGTTCTGCA CGTTGAGCAT
481 CTGCTCATCC ACCTCCTTCA TGGACATCCG TCCACGGAAG ANNNNNNNNN N
//
Primer: Rluc8-H_R (Pr1031)
Region: insert mid(Rluc8-m1 dN3,mVenus dC10)
Sequence file: RDB13338_A5E8d.seq check
>13338_13338_B1G9F_3_Rluc8-H_R_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTCCTCTTCC TCTGCTCGGG GTCGTACACC TTGGTACCCC
   61 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC
  121 TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA
  181 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCTCG ATGTTGTGGC
  241 GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA TAGACGTTGT
  301 GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA
  361 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG
  421 TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA
  481 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGGCG AAGCACTGCA
  541 GGCCGTAGCC CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC
  601 AGATCAGCTT CAGGGTCNGC TTGCCGTANG TGNNATCGCC CTCGCCCTCG CCGGANACGC
  661 TGAACTTGTG GNCNNTNACG TCNCNGTCNN NCTCNANCNN GANGGNNANN NNCCNNNNNA
721 NNGCNNNTNN CCNNNNNNNN NN
//
Primer: tube-B_F (Pr1030)
Region: insert mid(mouse Tubb5,mVenus dC10)
Sequence file: RDB13338_A5E8e.seq check
>13338_13338_B1G9F_3_tube-B_F_A02_02_ABI24.ab1
    1 NNNNNNNGNN NNNNNNNGAG GNNTTCGGAG AGGAGGCAGA AGAGGAGGCC CAGTCGACGG
   61 TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA
  121 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
  181 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA
  241 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC
  301 AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC
  361 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC
  421 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG
  481 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA
  541 ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCNAACTTC AAGATCCGCC
  601 ACAACATCGA GGANGGCGGC GTGCAGCTCG CCGANCACTA CCAGCAGAAC ACCCCCATCG
  661 GCGANNGCCC CNNGCTGCTG CCCGANNNCC NCTANNNGAN CTNNNNGTCC NNGCNNANNN
721 ANNNNCCNNN NNNNNNN
//
Primer: Rluc8-B_R (Pr1032)
Region: insert mid(Rluc8-m1 dN3,mVenus dC10)
Sequence file: RDB13338_A5E8f.seq check
>13338_13338_B1G9F_3_Rluc8-B_R_C02_08_ABI24.ab1
    1 NNNNNNNNNN NCTTTCTNNT GATCTTGCTG GGCAGCACGG TCTCCACGAA GAAGTTGTTC
   61 TCCAGCACCA TCTTCTCGCC CTCCTCGCTC TTGATCAGGG CGATGTCCTC CTCGATGTCT
  121 GGCCACTCGT CCCAGCTCTC GATCACGTCC ACCACGCTCT CCATGTGCAC GATGGCCTTG
  181 ATCCTGTCCT GGTGCTCGTA GGCGTAGTGG AAGGCCAGGG CGGCGCCCCA GTCGTGGCCC
  241 ACGAAGATGA TCTTCTTGGG CAGGTTCAGA AGCTCGAACC AGGCGGTCAG GTACTTGTAG
  301 TGGTCCAGCA GCCTGTAGCT GCCGTTGCCG CTCTTGCCGC TCTTGCCCAT GCCGATCAGA
  361 TCGGGGATGA TGCACCTGGC CACGGGCTCG ATGTGGGGCA CCACGTGCCT CCACAGGTAG
  421 CTGCTAGTGG CGTTGCCGTG CAGGAAGATC ACGGCGTTCT CGGCGTGCTT CTCGCTGTCG
  481 TAGTAGTTGA TGAAGCTGTC CAGCACGTTC ATCTGCTTGC ACCTGGCCCA CCACTGGGGG
  541 CCGGTGATCA TCCTCTTCCT CTGCTCGGGG TCGTACACCT TGGTACCCCC GGCGGCGGTC
  601 ACGAACTCCA GCANGANCNT GTGATCGCGC TTCTCGTTGG GGTCNTTGCT CANCTTGGAN
  661 TGNTANCTCN NNNANNNNNN GNCNGGCANC ANCACNGGGN NNNCNNNNAN GGGGGNNNNC
721 NGNNGGNNNN NNNNNN
//
Primer: Rluc8-M_F (Pr0580)
Region: insert mid(Rluc8-m1 dN3)
Sequence file: RDB13338_A5E8g.seq check
>13338_13338_B1G9F_3_Rluc8-M_F_20_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCNNNGAC NGGNTCAAGG CCATCGTGCA
   61 CATGGAGAGC GTGGTGGACG TGATCGAGAG CTGGGACGAG TGGCCAGACA TCGAGGAGGA
  121 CATCGCCCTG ATCAAGAGCG AGGAGGGCGA GAAGATGGTG CTGGAGAACA ACTTCTTCGT
  181 GGAGACCGTG CTGCCCAGCA AGATCATGAG AAAGCTGGAG CCCGAGGAGT TCGCCGCCTA
  241 CCTGGAGCCC TTCAAGGAGA AGGGCGAGGT GAGAAGACCC ACCCTGAGCT GGCCCAGAGA
  301 GATCCCCCTG GTGAAGGGCG GCAAGCCCGA CGTGGTGCAG ATCGTGAGAA ACTACAACGC
  361 CTACCTGAGA GCCAGCGACG ACCTGCCCAA GCTGTTCATC GAGGGCGACC CCGGCTTCTT
  421 CAGCAACGCC ATCGTGGAGG GCGCCAAGAA GTTCCCCAAC ACCGAGTTCG TGAAGGTGAA
  481 GGGCCTGCAC TTCCTCCAGG AGGACGCCCC CGACGAGATG GGCAAGTACN TNNAGAGCTT
  541 CGTGGAGAGA GTGCTGAANA NNNNGCANTA NGCGGCCGCG ACTCNANNTN NTAATCAGCC
  601 NTACNACATT TGTANAGGNT TTNCTTGCTT TAAAAANCCT CCCNCACCTC CCCCTGAACC
  661 NGAANNNNAA ATGNNTGCAN NNGNTGNTNG NNNACTNNNT ATNGNNNNNT TANAANGGNN
721 TNNNNNNNNA NNNNNANCNN N
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV 40pro_ori,NeoR_KanR
Sequence file: RDB13338_A5E8h.seq check
>13338_13338_B1G9F_3_SV40pro_ori_F_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNGCCGC CTCNGCCTCT GANCTATTCC NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT
  121 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG
  181 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC
  241 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA
  301 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT
  361 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA
  421 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG
  481 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT
  541 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA
  601 GCATCAGGGG CTCGCGCCNG CCGAACTGTT CGCCNGGCTC NAGGCNANCA TGCCCGACGG
  661 CGANGNNCTC GNCGTGANCC NNGNNNANGN CNGCNNGNNN ANNNCNNNGN NNNAANNGNN
721 NNCNTTNCNN NNNNCNTCNN CNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]