RDB13334 - pONL-N1

Resource data sheet
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pONL-N1_3xNLS

Orange Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40.

Catalog number	RDB13334
Resource name	pONL-N1_3xNLS
Clone info.	Expression vector of the Orange Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40. Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone	pEGFP-N1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli)
Gene/insert name	Synthetic three copies of SV40 nuclear localization signal
Depositor	Okada, Yasushi \| Takai, Akira \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供条件	本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] 宮脇敦史博士による提供承諾書 [Word]
備考	宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13334	pONL-N1_3xNLS	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content	Nano-lantern (English text)
Featured content	Nano-lantern (Japanese text)
Featured content	Organelle marker (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

2019.04.09

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