Orange Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40.
Catalog number | RDB13334 |
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Resource name | pONL-N1_3xNLS |
Clone info. | Expression vector of the Orange Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40. Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI. |
Vector backbone | pEGFP-N1 (Plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli) |
Gene/insert name | Synthetic three copies of SV40 nuclear localization signal |
Depositor | Okada, Yasushi | Takai, Akira | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS. |
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Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form [Word] by Dr. Atsushi Miyawaki |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13334 | pONL-N1_3xNLS | DNA solution |
Electronic file
Featured content
Featured content | Nano-lantern (English text) |
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Featured content | Nano-lantern (Japanese text) |
Featured content | Organelle marker (English text) |
References
original | Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. |
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User reports and related articles (go to bottom)
This clone will be sequenced a portion and digested by restriction enzyme for examination.
References
2019.04.09
GNP_filter3_RDBDEP_html_190327.pl