RDB13328 - pONL-C1

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pONL-C1 (#RDB13328)

Orange Nano-lantern to construct fusion proteins to the C-terminus.

Clone info.	Cloning and expression vector to construct fusion proteins to the C-terminus of Orange Nano-lantern.
Comment	Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone	pEGFP-C1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli)
Growth conditions	LB + Kan, 37C
Depositor\|Developer	Okada, Yasushi \| Takai, Akira \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13328	pONL-C1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pONL-C1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13328).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13328_B0Ihp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB13328_B0Iha.seq
>13328_13328_B0Ih_CMV-Forward_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGGCGG TANGCGTGTN NNGGTGGGNN GTCTATATAA 61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCCA CCATGGTGAG CGTGATCAAG 121 CCCGAGATGA AGATGAGGTA CTACATGGAC GGCTCCGTCA ATGGGCATGA GTTCACAATC 181 GAGGGTGAGG GCACAGGCAG ACCTTACGAG GGACATCAGG AGATGACACT GCGCGTCACA 241 ATGGCCGAGG GCGGGCCAAT GCCTTTCGCC TTCGACCTGG TGTCCCACGT GTTCTGTTAC 301 GGCCACAGAG TTTTTACTAA ATATCCAGAA GAGATCCCAG ACTATTTCAA GCAGGCCTTT 361 CCTGAGGGCC TGTCCTGGGA GAGGTCCCTG GAGTTCGAGG ACGGCGGCTC CGCCTCCGTG 421 AGCGCCCACA TCAGCCTGAG GGGCAACACC TTCTACCACA AGTCCAAGTT CACCGGCGTG 481 AACTTCCCCG CCGACGGCCC CATCATGCAG AACCAGAGCG TGGACTGGGA GCCCTCCACC 541 GAGAAGATCA CCGCCAGCGA CGGCGTGCTG AAGGGCGACG TGACCATGTA CCTGAAGCTG 601 GAGGGCGGCG GCAACCACAA GTGCCAGATG AAGACCACCT ACAAGGCCGC CAAGGAGATC 661 CTGGAGATGC CCGGCGACCA CTACATCGGC CACAGGCTGG TGAGGAAGAN CGANGGCAAC 721 ATCACCGANC AGGTGGANGA NGCCGNGGCC CACTCCGGTA CCAAGGTGTA CGACCCCGAN 781 CAACGCAAAC GCATGATCAC TNNNCCTCAN NGGTGGGCTC GCTGCAAGCA AATGAACGNG 841 CNNGANTCCT NCATCNACTA CTATNNNTNN NANAANCACG CNNNNAACGC CNNNNTTTTT 901 CNGNNTGGNN ACGCTACNNC CNNCTNNCNN NNNGNNNNNN NNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB13328_B0Ihb.seq
>13328_13328_B0Ih_SV40polyA_R2_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGTTNACAN CAACAATTGC ATTNNTTTTA TGTTTCAGGT 61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC 121 TGATTATGAT CAGTTATCTA GATCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACTGCAG 181 AATTCGAAGC TTGAGCTCGA GATCTGAGTC CGGACTGCTC GTTCTTCAGC ACGCGCTCCA 241 CGAAGCTCTT GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGAGG AAGTGGAGGC 301 CCTTCACCTT CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA ATAGCGTTGG 361 AAAAGAACCC AGGGTCGGAC TCGATGAACA GCTTAGGCAG ATCGTCGCTG GCCCGAAGGT 421 AGGCGTTGTA GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA ACGAGAGGGA 481 TCTCGCGAGG CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG AATGGCTCCA 541 GGTAGGCAGC GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGC AACAGGGTCT 601 CGACGAAGAA GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG ATCAGGGCCA 661 GCTCCTCCTC GATGTCANGC CACCCCATCC AGGACTCGAT CACGTCCACG ACACTCTCCA 721 TATGGACGAT GGCCTTGATC CTGTCTTGGT GCTCGTANGC GTANTGAAAG GCCAGANCGC 781 TCCCCCAGTC GTGGCCCACA AAGATGANTT TCTNNGNAAN GGNTCAGCAG CTCGAANNNA 841 NCGGNNNNGT ACTTGNANNG ATCCAGGANG NNATATGANC NATTCCNGCT CNNGNNNNNN 901 NTANCCNTTC CNATCANNNC NNNNNNNNNN //
Primer: mKO2-M_F (Pr0578) Region: insert mid Sequence file: RDB13328_B0Ihc.seq
>13328_13328_B0Ih_mKO2-M_F_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN CNANNGCNNG ATGAAGNNCA CCTACNAGGC CGCCNAGGAG 61 ATCCTGGAGA TGCCCGGCGA CCACTACATC GGCCACAGGC TGGTGAGGAA GACCGAGGGC 121 AACATCACCG AGCAGGTGGA GGACGCCGTG GCCCACTCCG GTACCAAGGT GTACGACCCC 181 GAGCAACGCA AACGCATGAT CACTGGGCCT CAGTGGTGGG CTCGCTGCAA GCAAATGAAC 241 GTGCTGGACT CCTTCATCAA CTACTATGAT TCCGAGAAGC ACGCCGAGAA CGCCGTGATT 301 TTTCTGCATG GTAACGCTAC CTCCAGCTAC CTGTGGAGGC ACGTCGTGCC TCACATCGAG 361 CCCGTGGCTA GATGCATCAT CCCTGATCTG ATCGGAATGG GTAAGTCCGG CAAGAGCGGG 421 AATGGCTCAT ATCGCCTCCT GGATCACTAC AAGTACCTCA CCGCTTGGTT CGAGCTGCTG 481 AACCTTCCAA AGAAAATCAT CTTTGTGGGC CACGACTGGG GGAGCGCTCT GGCCTTTCAC 541 TACGCCTACG AGCACCAAGA CAGGATCAAG GCCATCGTCC ATATGGAGAG TGTCGTGGAC 601 GTGATCGAGT CCTGGATGGG GTGGCCTGAC ATCNNNNAGG AGCTGGCCCT GATCAAGAGC 661 GAAGAGGGCG AGAAAATGGT GCTTGAGAAT AACTTCTTCG TCGAGACCCT GTTGCCAAGC 721 AAGATCATGC GGAAACTGGA GCCTGAGGAG TTCGCTGCCT ACCTGGAGCC ATTCNAGGAG 781 AANGGCGAGG TTAGACGGCC TACCCTCTCC TGNNCTCGCG AGATCCCTCT CGTTAANGGA 841 GGCAAGCCCG ACGNCGTCCA GATTGTCCGC AACTACNANN GCCTACCTTC NGGCCNNNCG 901 NCNATCTGCC NNAGCTGTNC ANCGAGTCCN ANCCNGGGGN TCTTTTNCNA NCNCTANTGT 961 CNAGGGAGCN NANAANNNTC CCTAANNNNC NANNTTNNNN AANGNNAANG GNNCNNNNNN 1021 NTNCNNNCAG GNANGNNGCT NNNNNANNAA AATGGGGNNN NNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB13328_B0Ihd.seq
>13328_13328_B0Ih_SV40pro_ori_F_C06_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGCCTCNNC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG 61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC 121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT 241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC 301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG 361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC 421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC 541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG 601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG 661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA 721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG 781 ACATAGCGTT GGCTACCCGN GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGANCGCT 841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCNCAGCG CATCGCNNTC TATCGCCTTC 901 TTGACNANTT CTTCTGANCG GGNNTCTGGG GGTNNGAAAT GNNCGANNNN NCGACGCCCA 961 ANCNNGNCAT CNCNAGANNN NGATTNNNNC NNNNNNCNTT CNANNAANNG NNNGGNNNNN 1021 NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]

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