RDB13327 - pONL-N1

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pONL-N1 (#RDB13327)

Orange Nano-lantern to construct fusion proteins to the N-terminus.

Clone info.	Cloning and expression vector to construct fusion proteins to the N-terminus of Orange Nano-lantern.
Comment	Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone	pEGFP-N1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli)
Depositor\|Developer	Okada, Yasushi \| Takai, Akira \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
備考	宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13327	pONL-N1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pONL-N1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13327).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13327_A5E8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB13327_A5E8a.seq
>13327_13327_A5E8_3_CMV_Forward_D03_12.ab1 1 NNNNNNNNNN NNNNTNNNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC 121 AAGCTTCGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGT CGCCACCATG 181 GTGAGCGTGA TCAAGCCCGA GATGAAGATG AGGTACTACA TGGACGGCTC CGTCAATGGG 241 CATGAGTTCA CAATCGAGGG TGAGGGCACA GGCAGACCTT ACGAGGGACA TCAGGAGATG 301 ACACTGCGCG TCACAATGGC CGAGGGCGGG CCAATGCCTT TCGCCTTCGA CCTGGTGTCC 361 CACGTGTTCT GTTACGGCCA CAGAGTTTTT ACTAAATATC CAGAAGAGAT CCCAGACTAT 421 TTCAAGCAGG CCTTTCCTGA GGGCCTGTCC TGGGAGAGGT CCCTGGAGTT CGAGGACGGC 481 GGCTCCGCCT CCGTGAGCGC CCACATCAGC CTGAGGGGCA ACACCTTCTA CCACAAGTCC 541 AAGTTCACCG GCGTGAACTT CCCCGCCGAC GGCCCCATCA TGCAGAACCA GAGCGTGGAC 601 TGGGAGCCCT CCACCGAGAA GATCACCGCC AGCGACGGCG TGCTGAAGGG CGACGTGACC 661 ATGTACCTGA AGCTGGAGGG CGGCGGCAAC CACAAGTGCC AGATGAAGAC CACCTACAAG 721 GCCGCCAAGG AGATCCTGGA GATGCCCGGC GACCACTACA TCGGCCACAG GCTGGTGAGG 781 AAGANCGAGG GCAACATCAC CGANCANGTG GANGANGCCG TGGCCCACTC CNGTACCNAN 841 GTGTACGACN NCGANCAACG CAAACGCATG ATCNCTGGGC CTCANNNNNG GGCTCGCTGC 901 NAGCAAATGA NCGNGCNNNN NNNCTTCNTC NNNNACNATN NNTNCNNNNA NNCNCGNNNN 961 NNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB13327_A5E8b.seq
>13327_13327_B0IiF_3_SV40polyA_R2_E11_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNTTNN NNANNNNNNN NNTGCATTNN NTTTANGTTT NNGGTTCNGG 61 GGGANGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT 121 ATGATCTAGA GTCGCGGCCG CCTACTGCTC GTTCTTCAGC ACGCGCTCCA CGAAGCTCTT 181 GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGAGG AAGTGGAGGC CCTTCACCTT 241 CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA ATAGCGTTGG AAAAGAACCC 301 AGGGTCGGAC TCGATGAACA GCTTAGGCAG ATCGTCGCTG GCCCGAAGGT AGGCGTTGTA 361 GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA ACGAGAGGGA TCTCGCGAGG 421 CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG AATGGCTCCA GGTAGGCAGC 481 GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGC AACAGGGTCT CGACGAAGAA 541 GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG ATCAGGGCCA GCTCCTCCTC 601 GATGTCAGGC CACCCCATCC AGGACTCGAT CACGTCCACG ACACTCTCCA TATGGACGAT 661 GGCCTTGATC CTGTCTTGNT GCTCGTANGC GTAGTGAAAG GNNAGAGCGC TCCCCCANTC 721 GTGGCCCACA AAGATGATTT TCTTTGGAAN NGTTCANCAG CTCNAACCAA GCGGTGAGGT 781 ACTTGNANTG ATCCNNGNAG GCGATATGAN CCATTCCCGC TCTTGCCGGA NTTACCCATT 841 CCNATCAGAT CNNGNNTGAN GCNTCTNNNC NNCNGGGCNC GNNNGNNAGG CANNNNNNGN 901 CNTNNNNNNN NNN //
Primer: mKO2-M_F (Pr0578) Region: insert mid Sequence file: RDB13327_A5E8c.seq
>13327_13327_B0IiF_3_mKO2-M_F_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGAN GAAGNNNNCC TACNAGGCCG CNNAGGAGNT 61 CCTGGAGANG CCCGGCGACC ACTACATCGG CCACAGGCTG GTGAGGAAGA CCGAGGGCAA 121 CATCACCGAG CAGGTGGAGG ACGCCGTGGC CCACTCCGGT ACCAAGGTGT ACGACCCCGA 181 GCAACGCAAA CGCATGATCA CTGGGCCTCA GTGGTGGGCT CGCTGCAAGC AAATGAACGT 241 GCTGGACTCC TTCATCAACT ACTATGATTC CGAGAAGCAC GCCGAGAACG CCGTGATTTT 301 TCTGCATGGT AACGCTACCT CCAGCTACCT GTGGAGGCAC GTCGTGCCTC ACATCGAGCC 361 CGTGGCTAGA TGCATCATCC CTGATCTGAT CGGAATGGGT AAGTCCGGCA AGAGCGGGAA 421 TGGCTCATAT CGCCTCCTGG ATCACTACAA GTACCTCACC GCTTGGTTCG AGCTGCTGAA 481 CCTTCCAAAG AAAATCATCT TTGTGGGCCA CGACTGGGGG AGCGCTCTGG CCTTTCACTA 541 CGCCTACGAG CACCAAGACA GGATCAAGGC CATCGTCCAT ATGGAGAGTG TCGTGGACGT 601 GATCGAGTCC TGGATGGGGT GGCCTGACAT CGAGGAGGAG CTGGCCCTGA TCAAGAGCGA 661 AGAGGGCGAG AAAATGGTGC TTGAGAATAA CTTCTTCGTC GAGACCCTGT TGCCAAGCAA 721 GATCATGCGG AANCTGGAGC CTGAGGAGTT CGCTGCCTAC CTGGAGCCAT TCAAGGAGAA 781 GGGCGAGGTT AGACGGCCTA CCCTCTCCTG GNCNTCGCGA GATCCCTCTC GTTAANGGAG 841 GCANNCCCGA CNTCGTCNAG ATTGTCCGCA ANNNCNNCGC CTACCTTCGG GNCAGCNNNN 901 NATNTGCCNN AANCTGTNNN TCNAGTCCNA NCCNGGGNNC NTTNNCNANN NCTNNNGTCG 961 ANGGGANCNN AAGAANTTNC CNTAACNCCN NNNNNGTGNA NNNTNNAANG NCNNCNNNNT 1021 NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB13327_A5E8d.seq
>13327_13327_B0IiF_3_SV40pro_ori_F_G11_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNTGAN CTNNTCNNGA AGTAGTGAGG ANGCTTTTTT GGAGGCCTAG 61 GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 121 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 181 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 241 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG 301 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 361 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 421 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 481 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 541 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 601 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA GGATCTCGTC 661 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 721 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC ANGACATAGC GTTGGCTACC 781 CGNGATATTG CTGAANANCT NGGCGGCGAA TGGGCTGACC GCTTCCTCNN GCTTTNCNGN 841 ATCNCNNCTC CCNATTCNCA NCNNATCNNC NNTCNATCNN CNNTCTNNNN NANNTNNNNN 901 NNANNGGNNN NNNNGGNNNT NNNNANNNNN NNANNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]

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