Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCNL-C1 (#RDB13326)

Cyan Nano-lantern to construct fusion proteins to the C-terminus.

Clone info.	Cloning and expression vector to construct fusion proteins to the C-terminus of Cyan Nano-lantern.
Comment	Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam.
Vector backbone	pEGFP-C1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli)
Depositor|Developer	Okada, Yasushi | Takai, Akira |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13326	pCNL-C1	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCNL-C1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13326).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13326_A5E8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB13326_A5E8a.seq
>13326_13326_A5E8_2_CMV_Forward_B03_06.ab1     1 NNNNNNNNNN NNNNTNGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGNGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCCAC CATGGTGAGC AAGGGCGAGG   121 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA   181 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT   241 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGTCCT   301 GGGGCGTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT   361 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT   421 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA   481 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACT   541 TTAGCGACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA   601 AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA   661 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCA   721 AGCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGANCG   781 CCGCCGGGGG TACCAAGGTG TACGACCNCG ANCAGANGAA NANGANGATC ACCGGCCNNC   841 ANNGGNNNNN NAGGNGCAAG CAGATGAACG TGCNNGNNAG CNNCATCAAC NACNNCNANA   901 GCNANAAGCA CGCCNNNAAN GCCNNNNNNT TNCNGCNCGG NNACGCCNCN NNCANCTANN 961 NNNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB13326_A5E8b.seq
>13326_13326_B0IiF_2_SV40polyA_R2_B11_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTTNNNA NNAACAATTG CATTNNTTTT ATGTTTCAGG    61 TTCAGGGGGA NGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG   121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA   181 GAATTCGAAG CTTGAGCTCG AGATCTGAGT CCGGACTGCT CGTTCTTCAG CACTCTCTCC   241 ACGAAGCTCT TGATGTACTT GCCCATCTCG TCGGGGGCGT CCTCCTGGAG GAAGTGCAGG   301 CCCTTCACCT TCACGAACTC GGTGTTGGGG AACTTCTTGG CGCCCTCCAC GATGGCGTTG   361 CTGAAGAAGC CGGGGTCGCC CTCGATGAAC AGCTTGGGCA GGTCGTCGCT GGCTCTCAGG   421 TAGGCGTTGT AGTTTCTCAC GATCTGCACC ACGTCGGGCT TGCCGCCCTT CACCAGGGGG   481 ATCTCTCTGG GCCAGCTCAG GGTGGGTCTT CTCACCTCGC CCTTCTCCTT GAAGGGCTCC   541 AGGTAGGCGG CGAACTCCTC GGGCTCCAGC TTTCTCATGA TCTTGCTGGG CAGCACGGTC   601 TCCACGAAGA ANTTGTTCTC CAGCACCATC TTCTCGCCCT CCTCGCTCTT GATCAGGGCG   661 ATGTCCTCCT CGATGTCTGG CCACTCGTCC CAGCTCTCGA TCACGTCCAC CACGCTCTCC   721 ATGTGCACGA TGGCCTTNAT CCTGTCCTGN TGCTCGTANG CGTANTGGAA NGCCAGGGCG   781 GCGCNNNAGT CNNGGCCCAC GAAGATGATC TTCTTGGGCA NGTTCNNAAN CTCNAACCAG   841 GNNGNCNNGT ACTTNNANNG NNNNNNNANC CNGNANCNNC CNTTNCNGCT CNTGNNGCTC 901 TNNNCNNNGN CGATNANATC NNNNNNGANG CANCNNNNNN NNNN //
Primer: mTurquoise2-M_F (Pr0577) Region: insert mid Sequence file: RDB13326_A5E8c.seq
>13326_13326_B0IiF_2_mTurquoise2-M_F_C11_08_ABI24.ab1     1 NNNNNNNNNN NNTNNNNNGN NNNAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG    61 ACAACCACTA CCTGAGCACC CAGTCCAAGC TGAGCAAAGA CCCCAACGAG AAGCGCGATC   121 ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGGGTAC CAAGGTGTAC GACCCCGAGC   181 AGAGGAAGAG GATGATCACC GGCCCCCAGT GGTGGGCCAG GTGCAAGCAG ATGAACGTGC   241 TGGACAGCTT CATCAACTAC TACGACAGCG AGAAGCACGC CGAGAACGCC GTGATCTTCC   301 TGCACGGCAA CGCCACTAGC AGCTACCTGT GGAGGCACGT GGTGCCCCAC ATCGAGCCCG   361 TGGCCAGGTG CATCATCCCC GATCTGATCG GCATGGGCAA GAGCGGCAAG AGCGGCAACG   421 GCAGCTACAG GCTGCTGGAC CACTACAAGT ACCTGACCGC CTGGTTCGAG CTTCTGAACC   481 TGCCCAAGAA GATCATCTTC GTGGGCCACG ACTGGGGCGC CGCCCTGGCC TTCCACTACG   541 CCTACGAGCA CCAGGACAGG ATCAAGGCCA TCGTGCACAT GGAGAGCGTG GTGGACGTGA   601 TCGAGAGCTG GGACGAGTGG CCAGACATCG AGGAGGACAT CGCCCTGATC NAGAGCGAGG   661 AGGGCGAGAA GATGGTGCTG GAGAACAACT TCTTCGTGNA NACCGTGCTG CCCAGCAAGA   721 TCATGAGAAA GCTGGAGCCC GANGANTTCG CCGCCTACCT GGAGCCCTTC AAGGAGAAGG   781 GCGAGGTGAN AAGANCCANC CTGAGCTGGC CNNNNNAGAT CCCCCNGGNG NANGGCGGCA   841 AGCCCGACNN NGNGCNNATC GNGANAAACT ACNACNNNNA CNNNANANNC NNNCGNNNNA 901 NNTGNNCNAN GCTNNTNNNN NNANGGNNGN NCCNNNGNNT NNNNNNNNNN //
Primer: RLuc8-M_F (Pr0580) Region: insert mid Sequence file: RDB13326_A5E8d.seq
>13326_13326_B0IiF_2_RLuc8-M_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNNNNNCNN NNNNNNGGNN AGGNTCNAGG CCNTCGTGCA    61 CATGGNGAGC GTGGTGGACG TGATCGAGAG CTGGGACGAG TGGCCAGACA TCGAGGAGGA   121 CATCGCCCTG ATCAAGAGCG AGGAGGGCGA GAAGATGGTG CTGGAGAACA ACTTCTTCGT   181 GGAGACCGTG CTGCCCAGCA AGATCATGAG AAAGCTGGAG CCCGAGGAGT TCGCCGCCTA   241 CCTGGAGCCC TTCAAGGAGA AGGGCGAGGT GAGAAGACCC ACCCTGAGCT GGCCCAGAGA   301 GATCCCCCTG GTGAAGGGCG GCAAGCCCGA CGTGGTGCAG ATCGTGAGAA ACTACAACGC   361 CTACCTGAGA GCCAGCGACG ACCTGCCCAA GCTGTTCATC GAGGGCGACC CCGGCTTCTT   421 CAGCAACGCC ATCGTGGAGG GCGCCAAGAA GTTCCCCAAC ACCGAGTTCG TGAAGGTGAA   481 GGGCCTGCAC TTCCTCCAGG AGGACGCCCC CGACNAGATG GGNNNNTACA TCAAGAGCTT 541 CGNNNNNNNN NTGCNNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB13326_A5E8e.seq
>13326_13326_B0IiF_2_SV40pro_ori_F_D11_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNCTCN GCCTCTGNNC TATTCNNGAA GTAGTGAGGA NGCTTTTTTG    61 GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT   121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT   181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG   241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC   301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC   361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC   421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG   481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG   541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT   601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAN   661 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC   721 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA NGACATAGCG   781 NTGGCTACCC GNGATATTGC TGAAGAGCTT GGCNGCNAAT GGGCTGANCG CTTCCNCNNG   841 CTTTACGGNA TCGCNNCNCC NGANTCNCAG CGCANCNNCN NTCTATCNNN NTCNNNNNGA 901 NNTNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]

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