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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCNL-C1

Cyan Nano-lantern to construct fusion proteins to the C-terminus.

Catalog number RDB13326
Resource name pCNL-C1
Clone info. Cloning and expression vector to construct fusion proteins to the C-terminus of Cyan Nano-lantern.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Depositor|Developer Okada, Yasushi | Takai, Akira |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13326 pCNL-C1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Nano-lantern (English text)
            Featured content Nano-lantern (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13326_A5E8p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,insert 5'
            Sequence file: RDB13326_A5E8a.seq check
            >13326_13326_A5E8_2_CMV_Forward_B03_06.ab1
                1 NNNNNNNNNN NNNNTNGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGNGG TCTATATAAG
               61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCCAC CATGGTGAGC AAGGGCGAGG
              121 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA
              181 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT
              241 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGTCCT
              301 GGGGCGTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT
              361 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT
              421 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
              481 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACT
              541 TTAGCGACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA
              601 AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
              661 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCA
              721 AGCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGANCG
              781 CCGCCGGGGG TACCAAGGTG TACGACCNCG ANCAGANGAA NANGANGATC ACCGGCCNNC
              841 ANNGGNNNNN NAGGNGCAAG CAGATGAACG TGCNNGNNAG CNNCATCAAC NACNNCNANA
              901 GCNANAAGCA CGCCNNNAAN GCCNNNNNNT TNCNGCNCGG NNACGCCNCN NNCANCTANN
            961 NNNNNNNN
            //
            Primer: SV40polyA_R2
            Region: insert 3'
            Sequence file: RDB13326_A5E8b.seq check
            >13326_13326_B0IiF_2_SV40polyA_R2_B11_05_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNTTNNNA NNAACAATTG CATTNNTTTT ATGTTTCAGG
               61 TTCAGGGGGA NGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
              121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
              181 GAATTCGAAG CTTGAGCTCG AGATCTGAGT CCGGACTGCT CGTTCTTCAG CACTCTCTCC
              241 ACGAAGCTCT TGATGTACTT GCCCATCTCG TCGGGGGCGT CCTCCTGGAG GAAGTGCAGG
              301 CCCTTCACCT TCACGAACTC GGTGTTGGGG AACTTCTTGG CGCCCTCCAC GATGGCGTTG
              361 CTGAAGAAGC CGGGGTCGCC CTCGATGAAC AGCTTGGGCA GGTCGTCGCT GGCTCTCAGG
              421 TAGGCGTTGT AGTTTCTCAC GATCTGCACC ACGTCGGGCT TGCCGCCCTT CACCAGGGGG
              481 ATCTCTCTGG GCCAGCTCAG GGTGGGTCTT CTCACCTCGC CCTTCTCCTT GAAGGGCTCC
              541 AGGTAGGCGG CGAACTCCTC GGGCTCCAGC TTTCTCATGA TCTTGCTGGG CAGCACGGTC
              601 TCCACGAAGA ANTTGTTCTC CAGCACCATC TTCTCGCCCT CCTCGCTCTT GATCAGGGCG
              661 ATGTCCTCCT CGATGTCTGG CCACTCGTCC CAGCTCTCGA TCACGTCCAC CACGCTCTCC
              721 ATGTGCACGA TGGCCTTNAT CCTGTCCTGN TGCTCGTANG CGTANTGGAA NGCCAGGGCG
              781 GCGCNNNAGT CNNGGCCCAC GAAGATGATC TTCTTGGGCA NGTTCNNAAN CTCNAACCAG
              841 GNNGNCNNGT ACTTNNANNG NNNNNNNANC CNGNANCNNC CNTTNCNGCT CNTGNNGCTC
            901 TNNNCNNNGN CGATNANATC NNNNNNGANG CANCNNNNNN NNNN
            //
            Primer: mTurquoise2-M_F
            Region: insert mid
            Sequence file: RDB13326_A5E8c.seq check
            >13326_13326_B0IiF_2_mTurquoise2-M_F_C11_08_ABI24.ab1
                1 NNNNNNNNNN NNTNNNNNGN NNNAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG
               61 ACAACCACTA CCTGAGCACC CAGTCCAAGC TGAGCAAAGA CCCCAACGAG AAGCGCGATC
              121 ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGGGTAC CAAGGTGTAC GACCCCGAGC
              181 AGAGGAAGAG GATGATCACC GGCCCCCAGT GGTGGGCCAG GTGCAAGCAG ATGAACGTGC
              241 TGGACAGCTT CATCAACTAC TACGACAGCG AGAAGCACGC CGAGAACGCC GTGATCTTCC
              301 TGCACGGCAA CGCCACTAGC AGCTACCTGT GGAGGCACGT GGTGCCCCAC ATCGAGCCCG
              361 TGGCCAGGTG CATCATCCCC GATCTGATCG GCATGGGCAA GAGCGGCAAG AGCGGCAACG
              421 GCAGCTACAG GCTGCTGGAC CACTACAAGT ACCTGACCGC CTGGTTCGAG CTTCTGAACC
              481 TGCCCAAGAA GATCATCTTC GTGGGCCACG ACTGGGGCGC CGCCCTGGCC TTCCACTACG
              541 CCTACGAGCA CCAGGACAGG ATCAAGGCCA TCGTGCACAT GGAGAGCGTG GTGGACGTGA
              601 TCGAGAGCTG GGACGAGTGG CCAGACATCG AGGAGGACAT CGCCCTGATC NAGAGCGAGG
              661 AGGGCGAGAA GATGGTGCTG GAGAACAACT TCTTCGTGNA NACCGTGCTG CCCAGCAAGA
              721 TCATGAGAAA GCTGGAGCCC GANGANTTCG CCGCCTACCT GGAGCCCTTC AAGGAGAAGG
              781 GCGAGGTGAN AAGANCCANC CTGAGCTGGC CNNNNNAGAT CCCCCNGGNG NANGGCGGCA
              841 AGCCCGACNN NGNGCNNATC GNGANAAACT ACNACNNNNA CNNNANANNC NNNCGNNNNA
            901 NNTGNNCNAN GCTNNTNNNN NNANGGNNGN NCCNNNGNNT NNNNNNNNNN
            //
            Primer: RLuc8-M_F
            Region: insert mid
            Sequence file: RDB13326_A5E8d.seq check
            >13326_13326_B0IiF_2_RLuc8-M_F_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCNNNNNCNN NNNNNNGGNN AGGNTCNAGG CCNTCGTGCA
               61 CATGGNGAGC GTGGTGGACG TGATCGAGAG CTGGGACGAG TGGCCAGACA TCGAGGAGGA
              121 CATCGCCCTG ATCAAGAGCG AGGAGGGCGA GAAGATGGTG CTGGAGAACA ACTTCTTCGT
              181 GGAGACCGTG CTGCCCAGCA AGATCATGAG AAAGCTGGAG CCCGAGGAGT TCGCCGCCTA
              241 CCTGGAGCCC TTCAAGGAGA AGGGCGAGGT GAGAAGACCC ACCCTGAGCT GGCCCAGAGA
              301 GATCCCCCTG GTGAAGGGCG GCAAGCCCGA CGTGGTGCAG ATCGTGAGAA ACTACAACGC
              361 CTACCTGAGA GCCAGCGACG ACCTGCCCAA GCTGTTCATC GAGGGCGACC CCGGCTTCTT
              421 CAGCAACGCC ATCGTGGAGG GCGCCAAGAA GTTCCCCAAC ACCGAGTTCG TGAAGGTGAA
              481 GGGCCTGCAC TTCCTCCAGG AGGACGCCCC CGACNAGATG GGNNNNTACA TCAAGAGCTT
            541 CGNNNNNNNN NTGCNNNNNN NNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,NeoR_KanR
            Sequence file: RDB13326_A5E8e.seq check
            >13326_13326_B0IiF_2_SV40pro_ori_F_D11_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNCTCN GCCTCTGNNC TATTCNNGAA GTAGTGAGGA NGCTTTTTTG
               61 GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT
              121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
              181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
              241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
              301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC
              361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC
              421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG
              481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG
              541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT
              601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAN
              661 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC
              721 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA NGACATAGCG
              781 NTGGCTACCC GNGATATTGC TGAAGAGCTT GGCNGCNAAT GGGCTGANCG CTTCCNCNNG
              841 CTTTACGGNA TCGCNNCNCC NGANTCNCAG CGCANCNNCN NTCTATCNNN NTCNNNNNGA
            901 NNTNNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
            reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl