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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCNL-N1

Cyan Nano-lantern to construct fusion proteins to the N-terminus.

Catalog number RDB13325
Resource name pCNL-N1
Clone info. Cloning and expression vector to construct fusion proteins to the N-terminus of Cyan Nano-lantern.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Depositor|Developer Okada, Yasushi | Takai, Akira |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13325 pCNL-N1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Nano-lantern (English text)
            Featured content Nano-lantern (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13325_A5E8p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,insert 5'
            Sequence file: RDB13325_A5E8a.seq check
            >13325_13325_A5E8_1_CMV_Forward_G12_21.ab1
                1 NNNNNNNNNN NNNNNNNNNN NTGNNGCNNN TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
               61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG ACTCAGATCT
              121 CGAGCTCAAG CTTCGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGTCGC
              181 CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
              241 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
              301 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
              361 CACCCTCGTG ACCACCCTGT CCTGGGGCGT GCAGTGCTTC GCCCGCTACC CCGACCACAT
              421 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT
              481 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
              541 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
              601 GCACAAGCTG GAGTACAACT ACTTTAGCGA CAACGTCTAT ATCACCGCCG ACAAGCAGAA
              661 GAACGGCATC AAGGCCAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT
              721 CGCCGANCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
              781 CCACTACCTG ANCACCCAGT CCAAGCTGAN CAAAGACCCC AACGAGAAGC GCGATCACAT
              841 GGTCCTGCTG GANTTCGTGA NCGCCGCCGG GGGTNNCAAG GTGTACGANC CCNANCAGAN
              901 GAAGANGANG ATCANCGGCC CCCAGNGGNG GGCCANGNGC AAGCANATGA ACNNGCTNGA
              961 NAGCTNNNTN NNNNNCNACN ANNNCGANAA NNCNNGCNNN ANNNNNNCNN NNNNNNTNNC
            1021 NNNNNNNNNN
            //
            Primer: SV40polyA_R2
            Region: insert 3'
            Sequence file: RDB13325_A5E8b.seq check
            >13325_13325_B0IiF_1_SV40polyA_R2__D11_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNTAANNN NAACAATTGC ATTCNTTTTA TGTTTCAGGT
               61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
              121 TGATTATGAT CTAGAGTCGC GGCCGCCTAC TGCTCGTTCT TCAGCACTCT CTCCACGAAG
              181 CTCTTGATGT ACTTGCCCAT CTCGTCGGGG GCGTCCTCCT GGAGGAAGTG CAGGCCCTTC
              241 ACCTTCACGA ACTCGGTGTT GGGGAACTTC TTGGCGCCCT CCACGATGGC GTTGCTGAAG
              301 AAGCCGGGGT CGCCCTCGAT GAACAGCTTG GGCAGGTCGT CGCTGGCTCT CAGGTAGGCG
              361 TTGTAGTTTC TCACGATCTG CACCACGTCG GGCTTGCCGC CCTTCACCAG GGGGATCTCT
              421 CTGGGCCAGC TCAGGGTGGG TCTTCTCACC TCGCCCTTCT CCTTGAAGGG CTCCAGGTAG
              481 GCGGCGAACT CCTCGGGCTC CAGCTTTCTC ATGATCTTGC TGGGCAGCAC GGTCTCCACG
              541 AAGAAGTTGT TCTCCAGCAC CATCTTCTCG CCCTCCTCGC TCTTGATCAG GGCGATGTCC
              601 TCCTCGATGT CTGGCCACTC GTCCCAGCTC TCGATCACGT CCACCACGCT CTCCATGTGC
              661 ACGATGGCCT TGATCCTGTC CTGGTGCTCG TAGGCGTAGT GGAAGGCCAG GGCGGCGCCC
              721 CAGTCGTGGC CCACGAAGAT GATCTTCTTG GGCANGTTCA GAAGCTCGAA CCAGGCGGTC
              781 AGGTACTTGT AGTGGTCCAG CAGCCTGTAG CTGCCGTTGC CGCTCTTGCC GCTCTTGCCC
              841 NTGCCGATCA NATCNGGGAN GATGCACCTG GCCNCGGGCT CGATGTGGGG CACCACGNGC
              901 CTCNNCAGGN AGCNGCNNNN GGCGNNNCCG TGCNNNAANA TCACGGNNTT CTCNGNGTGC
            961 TNCTCGCNGN CNNANNNNTT GANGNNNCTN NNCNNNNNNN NNN
            //
            Primer: mTurquoise2-M_F
            Region: insert mid
            Sequence file: RDB13325_A5E8c.seq check
            >13325_13325_B0IiF_1_mTurquoise2-M_F_H10_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NGCANNACAC CCCCATCGGC GNNGGCCCCG
               61 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCAA GCTGAGCAAA GACCCCAACG
              121 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGGGT ACCAAGGTGT
              181 ACGACCCCGA GCAGAGGAAG AGGATGATCA CCGGCCCCCA GTGGTGGGCC AGGTGCAAGC
              241 AGATGAACGT GCTGGACAGC TTCATCAACT ACTACGACAG CGAGAAGCAC GCCGAGAACG
              301 CCGTGATCTT CCTGCACGGC AACGCCACTA GCAGCTACCT GTGGAGGCAC GTGGTGCCCC
              361 ACATCGAGCC CGTGGCCAGG TGCATCATCC CCGATCTGAT CGGCATGGGC AAGAGCGGCA
              421 AGAGCGGCAA CGGCAGCTAC AGGCTGCTGG ACCACTACAA GTACCTGACC GCCTGGTTCG
              481 AGCTTCTGAA CCTGCCCAAG AAGATCATCT TCGTGGGCCA CGACTGGGGC GCCGCCCTGG
              541 CCTTCCACTA CGCCTACGAG CACCAGGACA GGATCAAGGC CATCGTGCAC ATGGAGAGCG
              601 TGGTGGACGT GATCGAGAGC TGGGACGAGT GGCCAGACAT CGAGGAGGAC ATCGCCCTGN
              661 TCAAGAGCGA GGAGGGCGAG AAGATGGTGC TGGANAACAA CTTCTTCGTG GANACCGTGC
              721 TGCCCAGCAA GATCATGAGA AAGCTGGAGC CCGANGANTT CGCCGCCTAC CNGGAGCCNT
              781 TCAAGGAGAA NNGNCGAGGT GNNAANNACC CACCCTGANC TGGCCNNNAN NNNNNCCCCC
              841 NGGNNNNNNG GCGGNNAGCC CNANNNGGNG NANATCGNGA GAAACTACNA NNNNNANNNN
            901 NNNNNCNGCG ACNNNNNNNN NN
            //
            Primer: SV40pro_ori_F
            Region: NeoR_KanR
            Sequence file: RDB13325_A5E8d.seq check
            >13325_13325_B0IiF_1_SV40pro_ori_F_A11_02_ABI24.ab1
                1 NNNNNNNNNN NNNNCNNNNN NNGCCTCTGN NCTANTCNNG AANTAGTGNN GAGGCTTTTT
               61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
              121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
              181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
              241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
              301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
              361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
              421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
              481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
              541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC
              601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG
              661 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC
              721 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGNTGTGGC GGANCGCTAT CNNNCATAGC
              781 GTTGGCTACC CNNGATATTG CTGAAGANCT TGGCGGCNAA NNGGGCTGAN NGCTNCCTCN
              841 NGCTTTACGG TATCNNCNNC TCCNNATTCG CANNNNNTCG NCNTTCTATC NNCNTNNNTN
            901 NANNANTTNN NNTGANNNNN NNTNNNGGNN NTNNNNANNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
            reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl