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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCNL-N1 (#RDB13325)

Cyan Nano-lantern to construct fusion proteins to the N-terminus.

Clone info. Cloning and expression vector to construct fusion proteins to the N-terminus of Cyan Nano-lantern.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Depositor|Developer Okada, Yasushi | Takai, Akira |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。

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RDB13325 pCNL-N1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCNL-N1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13325).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13325_A5E8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'
Sequence file: RDB13325_A5E8a.seq check
>13325_13325_A5E8_1_CMV_Forward_G12_21.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTGNNGCNNN TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG ACTCAGATCT
  121 CGAGCTCAAG CTTCGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGTCGC
  181 CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
  241 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
  301 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
  361 CACCCTCGTG ACCACCCTGT CCTGGGGCGT GCAGTGCTTC GCCCGCTACC CCGACCACAT
  421 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT
  481 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
  541 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
  601 GCACAAGCTG GAGTACAACT ACTTTAGCGA CAACGTCTAT ATCACCGCCG ACAAGCAGAA
  661 GAACGGCATC AAGGCCAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT
  721 CGCCGANCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
  781 CCACTACCTG ANCACCCAGT CCAAGCTGAN CAAAGACCCC AACGAGAAGC GCGATCACAT
  841 GGTCCTGCTG GANTTCGTGA NCGCCGCCGG GGGTNNCAAG GTGTACGANC CCNANCAGAN
  901 GAAGANGANG ATCANCGGCC CCCAGNGGNG GGCCANGNGC AAGCANATGA ACNNGCTNGA
  961 NAGCTNNNTN NNNNNCNACN ANNNCGANAA NNCNNGCNNN ANNNNNNCNN NNNNNNTNNC
1021 NNNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB13325_A5E8b.seq check
>13325_13325_B0IiF_1_SV40polyA_R2__D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTAANNN NAACAATTGC ATTCNTTTTA TGTTTCAGGT
   61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  121 TGATTATGAT CTAGAGTCGC GGCCGCCTAC TGCTCGTTCT TCAGCACTCT CTCCACGAAG
  181 CTCTTGATGT ACTTGCCCAT CTCGTCGGGG GCGTCCTCCT GGAGGAAGTG CAGGCCCTTC
  241 ACCTTCACGA ACTCGGTGTT GGGGAACTTC TTGGCGCCCT CCACGATGGC GTTGCTGAAG
  301 AAGCCGGGGT CGCCCTCGAT GAACAGCTTG GGCAGGTCGT CGCTGGCTCT CAGGTAGGCG
  361 TTGTAGTTTC TCACGATCTG CACCACGTCG GGCTTGCCGC CCTTCACCAG GGGGATCTCT
  421 CTGGGCCAGC TCAGGGTGGG TCTTCTCACC TCGCCCTTCT CCTTGAAGGG CTCCAGGTAG
  481 GCGGCGAACT CCTCGGGCTC CAGCTTTCTC ATGATCTTGC TGGGCAGCAC GGTCTCCACG
  541 AAGAAGTTGT TCTCCAGCAC CATCTTCTCG CCCTCCTCGC TCTTGATCAG GGCGATGTCC
  601 TCCTCGATGT CTGGCCACTC GTCCCAGCTC TCGATCACGT CCACCACGCT CTCCATGTGC
  661 ACGATGGCCT TGATCCTGTC CTGGTGCTCG TAGGCGTAGT GGAAGGCCAG GGCGGCGCCC
  721 CAGTCGTGGC CCACGAAGAT GATCTTCTTG GGCANGTTCA GAAGCTCGAA CCAGGCGGTC
  781 AGGTACTTGT AGTGGTCCAG CAGCCTGTAG CTGCCGTTGC CGCTCTTGCC GCTCTTGCCC
  841 NTGCCGATCA NATCNGGGAN GATGCACCTG GCCNCGGGCT CGATGTGGGG CACCACGNGC
  901 CTCNNCAGGN AGCNGCNNNN GGCGNNNCCG TGCNNNAANA TCACGGNNTT CTCNGNGTGC
961 TNCTCGCNGN CNNANNNNTT GANGNNNCTN NNCNNNNNNN NNN
//
Primer: mTurquoise2-M_F (Pr0577)
Region: insert mid
Sequence file: RDB13325_A5E8c.seq check
>13325_13325_B0IiF_1_mTurquoise2-M_F_H10_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NGCANNACAC CCCCATCGGC GNNGGCCCCG
   61 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCAA GCTGAGCAAA GACCCCAACG
  121 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGGGT ACCAAGGTGT
  181 ACGACCCCGA GCAGAGGAAG AGGATGATCA CCGGCCCCCA GTGGTGGGCC AGGTGCAAGC
  241 AGATGAACGT GCTGGACAGC TTCATCAACT ACTACGACAG CGAGAAGCAC GCCGAGAACG
  301 CCGTGATCTT CCTGCACGGC AACGCCACTA GCAGCTACCT GTGGAGGCAC GTGGTGCCCC
  361 ACATCGAGCC CGTGGCCAGG TGCATCATCC CCGATCTGAT CGGCATGGGC AAGAGCGGCA
  421 AGAGCGGCAA CGGCAGCTAC AGGCTGCTGG ACCACTACAA GTACCTGACC GCCTGGTTCG
  481 AGCTTCTGAA CCTGCCCAAG AAGATCATCT TCGTGGGCCA CGACTGGGGC GCCGCCCTGG
  541 CCTTCCACTA CGCCTACGAG CACCAGGACA GGATCAAGGC CATCGTGCAC ATGGAGAGCG
  601 TGGTGGACGT GATCGAGAGC TGGGACGAGT GGCCAGACAT CGAGGAGGAC ATCGCCCTGN
  661 TCAAGAGCGA GGAGGGCGAG AAGATGGTGC TGGANAACAA CTTCTTCGTG GANACCGTGC
  721 TGCCCAGCAA GATCATGAGA AAGCTGGAGC CCGANGANTT CGCCGCCTAC CNGGAGCCNT
  781 TCAAGGAGAA NNGNCGAGGT GNNAANNACC CACCCTGANC TGGCCNNNAN NNNNNCCCCC
  841 NGGNNNNNNG GCGGNNAGCC CNANNNGGNG NANATCGNGA GAAACTACNA NNNNNANNNN
901 NNNNNCNGCG ACNNNNNNNN NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: NeoR_KanR
Sequence file: RDB13325_A5E8d.seq check
>13325_13325_B0IiF_1_SV40pro_ori_F_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNNNN NNGCCTCTGN NCTANTCNNG AANTAGTGNN GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
  121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
  181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
  241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
  301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
  361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
  421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
  481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
  541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC
  601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG
  661 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC
  721 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGNTGTGGC GGANCGCTAT CNNNCATAGC
  781 GTTGGCTACC CNNGATATTG CTGAAGANCT TGGCGGCNAA NNGGGCTGAN NGCTNCCTCN
  841 NGCTTTACGG TATCNNCNNC TCCNNATTCG CANNNNNTCG NCNTTCTATC NNCNTNNNTN
901 NANNANTTNN NNTGANNNNN NNTNNNGGNN NTNNNNANNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]