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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pYNL-C1

Yellow Nano-lantern to construct fusion proteins to the C-terminus.

Catalog number RDB13324
Resource name pYNL-C1
Clone info. Cloning and expression vector to construct fusion proteins to the C-terminus of Yellow Nano-lantern.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. YNL was provided by Dr. Takeharu Nagai, Osaka University.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Depositor|Developer Okada, Yasushi | Takai, Akira |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
            付加的提供条件 利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13324 pYNL-C1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Nano-lantern (English text)
            Featured content Nano-lantern (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13324_A5E8p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,insert 5'
            Sequence file: RDB13324_A5E8a.seq check
            >13324_13324_B0IiF_1_CMV_Forward_C10_07_ABI24.ab1
                1 NNNNNNNNCN NNNNNNNNNN NGNNNAATGG GCGGTANGCG TGTACGGTGG GNNGTCTATA
               61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CCACCATGGT GAGCAAGGGC
              121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
              181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
              241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
              301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
              361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
              421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
              481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
              541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
              601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
              661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GNNCTACCAG
              721 TCCAAGCTGA NCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT NGANTTCGTG
              781 ACCGCCNCCN GGGGTACCAA GGNGTACNAN CCNNANCANA NGAAGANGAT GATCACCGGC
              841 CCCCANNGGN GGGNCANGNN CNNGCANATG AANNNNNCTG GACNNCTTCN TCNACNACTA
            901 CNNNANCNNN AANCNNNNNN NNNANNNNNN NNNN
            //
            Primer: ME-1290RV
            Region: SV40pA,insert 3'
            Sequence file: RDB13324_A5E8b.seq check
            >13324_13324_B0IiF_1_SV40polyA_R2__C11_08_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNGTTANN NNNNACAATT GCATTCNTTT TATGTTTNNG
               61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
              121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
              181 AGAATTCGAA GCTTGAGCTC GAGATCTGAG TCCGGACTGC TCGTTCTTCA GCACTCTCTC
              241 CACGAAGCTC TTGATGTACT TGCCCATCTC GTCGGGGGCG TCCTCCTGGA GGAAGTGCAG
              301 GCCCTTCACC TTCACGAACT CGGTGTTGGG GAACTTCTTG GCGCCCTCCA CGATGGCGTT
              361 GCTGAAGAAG CCGGGGTCGC CCTCGATGAA CAGCTTGGGC AGGTCGTCGC TGGCTCTCAG
              421 GTAGGCGTTG TAGTTTCTCA CGATCTGCAC CACGTCGGGC TTGCCGCCCT TCACCAGGGG
              481 GATCTCTCTG GGCCAGCTCA GGGTGGGTCT TCTCACCTCG CCCTTCTCCT TGAAGGGCTC
              541 CAGGTAGGCG GCGAACTCCT CGGGCTCCAG CTTTCTCATG ATCTTGCTGG GCAGCACGGT
              601 CTCCACGAAG AAGTTGTTCT CCAGCACCAT CTTCTCGCCC TCCTCGCTCT TGATCAGGGC
              661 GATGTCCTCC TCGATGTCTG GCCACTCGTC CCAGCTCTCG ATCACGTCCA NNNNNNTCTC
              721 CATGTGCACG ATGGCCTTGA TCCTGTCCTG GTGCTCGTAN GCGTAGTGGA ANGNNAGGGC
              781 GGCGCCCCAG TCGTGGCCCA CNAAGATGAT CTTCTTGNGC ANGTTCANAN NNNCGAANNN
              841 NNNGGTCANG TACTTGTANT GGTCCANCAN CCNGTAGCTG CCNGTTGNCN GCTCTNGNCG
              901 CTCTTGCCCN NNNNNANTCA GATCGGGGAA NGANGCANCT GGGCCNCNGG NCTNNATGTG
              961 GGGNNACNNC GNGNNNNNCN CAGGTNNNNT GCNNNNGGNN NTTGCCNNGC NNGNNNNNNN
            1021 ACNGNNNNTN NNNGNNNNNN NNNN
            //
            Primer: mTurquoise2-M_F
            Region: insert mid
            Sequence file: RDB13324_A5E8c.seq check
            >13324_13324_B0IiF_1_mTurqoise2-M_F_G03_21_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNCC NNNCNNNTAC NNGNAGANCA CCCCCATCGG CGANGGCCCC
               61 GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCA AGCTGAGCAA AGACCCCAAC
              121 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGGG TACCAAGGTG
              181 TACGACCCCG AGCAGAGGAA GAGGATGATC ACCGGCCCCC AGTGGTGGGC CAGGTGCAAG
              241 CAGATGAACG TGCTGGACAG CTTCATCAAC TACTACGACA GCGAGAAGCA CGCCGAGAAC
              301 GCCGTGATCT TCCTGCACGG CAACGCCACT AGCAGCTACC TGTGGAGGCA CGTGGTGCCC
              361 CACATCGAGC CCGTGGCCAG GTGCATCATC CCCGATCTGA TCGGCATGGG CAAGAGCGGC
              421 AAGAGCGGCA ACGGCAGCTA CAGGCTGCTG GACCACTACA AGTACCTGAC CGCCTGGTTC
              481 GAGCTTCTGA ACCTGCCCAA GAAGATCATC TTCGTGGGCC ACGACTGGGG CGCCGCCCTG
              541 GCCTTCCACT ACGCCTACGA GCACCAGGAC AGGATCAAGG CCATCGTGCA CATGGAGAGC
              601 GTGGTGGACG TGATCGAGAG CTGGGACGAG TGGCCAGACA TCGAGGAGGA CATCGCCCTG
              661 ATCAAGANCG AGGAGGGCGA NAAGATGGTG CTGGAGANCA ACTTCNTTCG TGGAGACCGT
              721 GCTGCCCAGC NANGATNATG AGAANNNCTG GANCCCCGAN GANTTCGNCC GCCCTACCTG
              781 GANCCCNTTC NAGGNNNANN GNCNAGGNGN NNANNANNCN NNCNNNNNNC TGGGNCCNNN
              841 ANANNATCCC CCNTGNNNNA AGGGCNGGNN ANGCCCCNNN NGTGNTGNCA ANANNNNNNG
              901 NNNNAAANTA NNNNNNNCNN TANNNNNNNA NNCNNGNNNN NNANNCNNNG NNNNNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,NeoR_KanR
            Sequence file: RDB13324_A5E8d.seq check
            >13324_13324_B0IiF_1_SV40pro_ori_F_F10_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNG CCTCNNCCTC TGNNCTANTC NNGAAGTAGT GNGGANGCTT
               61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
              121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
              181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
              241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
              301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
              361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
              421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
              481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
              541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
              601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
              661 GCGAGGATCT CGTCGTGANC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
              721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACA
              781 TAGCGTTGGC TACCCGNGAT ATTGCTGAAG ANCTTGNNGG CNAATGGGCT GANCGCTTCC
              841 TCNNNNTTTA NNGTATCGCC NCTNCNNANN NGCANNGCAT CNNCNTTCTA TCNNCNNNCT
            901 TGANNANNTN CNTNCTNANN NGGNNNNNTN GGGGNTNNNA AANNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
            reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl