Please review the QC test results indicated by icon below as well as clone information before placing your order.

pYNL-C1 (#RDB13324)

Yellow Nano-lantern to construct fusion proteins to the C-terminus.

Clone info.	Cloning and expression vector to construct fusion proteins to the C-terminus of Yellow Nano-lantern.
Comment	Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. YNL was provided by Dr. Takeharu Nagai, Osaka University.
Vector backbone	pEGFP-C1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli)
Growth conditions	LB + Kan, 37C
Depositor|Developer	Okada, Yasushi | Takai, Akira |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13324	pYNL-C1	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pYNL-C1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13324).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13324_A5E8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB13324_A5E8a.seq
>13324_13324_B0IiF_1_CMV_Forward_C10_07_ABI24.ab1     1 NNNNNNNNCN NNNNNNNNNN NGNNNAATGG GCGGTANGCG TGTACGGTGG GNNGTCTATA    61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CCACCATGGT GAGCAAGGGC   121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC   601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG   661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GNNCTACCAG   721 TCCAAGCTGA NCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT NGANTTCGTG   781 ACCGCCNCCN GGGGTACCAA GGNGTACNAN CCNNANCANA NGAAGANGAT GATCACCGGC   841 CCCCANNGGN GGGNCANGNN CNNGCANATG AANNNNNCTG GACNNCTTCN TCNACNACTA 901 CNNNANCNNN AANCNNNNNN NNNANNNNNN NNNN //
Primer: ME-1290RV (Pr0046) Region: SV40pA,insert 3' Sequence file: RDB13324_A5E8b.seq
>13324_13324_B0IiF_1_SV40polyA_R2__C11_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGTTANN NNNNACAATT GCATTCNTTT TATGTTTNNG    61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG   121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC   181 AGAATTCGAA GCTTGAGCTC GAGATCTGAG TCCGGACTGC TCGTTCTTCA GCACTCTCTC   241 CACGAAGCTC TTGATGTACT TGCCCATCTC GTCGGGGGCG TCCTCCTGGA GGAAGTGCAG   301 GCCCTTCACC TTCACGAACT CGGTGTTGGG GAACTTCTTG GCGCCCTCCA CGATGGCGTT   361 GCTGAAGAAG CCGGGGTCGC CCTCGATGAA CAGCTTGGGC AGGTCGTCGC TGGCTCTCAG   421 GTAGGCGTTG TAGTTTCTCA CGATCTGCAC CACGTCGGGC TTGCCGCCCT TCACCAGGGG   481 GATCTCTCTG GGCCAGCTCA GGGTGGGTCT TCTCACCTCG CCCTTCTCCT TGAAGGGCTC   541 CAGGTAGGCG GCGAACTCCT CGGGCTCCAG CTTTCTCATG ATCTTGCTGG GCAGCACGGT   601 CTCCACGAAG AAGTTGTTCT CCAGCACCAT CTTCTCGCCC TCCTCGCTCT TGATCAGGGC   661 GATGTCCTCC TCGATGTCTG GCCACTCGTC CCAGCTCTCG ATCACGTCCA NNNNNNTCTC   721 CATGTGCACG ATGGCCTTGA TCCTGTCCTG GTGCTCGTAN GCGTAGTGGA ANGNNAGGGC   781 GGCGCCCCAG TCGTGGCCCA CNAAGATGAT CTTCTTGNGC ANGTTCANAN NNNCGAANNN   841 NNNGGTCANG TACTTGTANT GGTCCANCAN CCNGTAGCTG CCNGTTGNCN GCTCTNGNCG   901 CTCTTGCCCN NNNNNANTCA GATCGGGGAA NGANGCANCT GGGCCNCNGG NCTNNATGTG   961 GGGNNACNNC GNGNNNNNCN CAGGTNNNNT GCNNNNGGNN NTTGCCNNGC NNGNNNNNNN 1021 ACNGNNNNTN NNNGNNNNNN NNNN //
Primer: mTurquoise2-M_F (Pr0577) Region: insert mid Sequence file: RDB13324_A5E8c.seq
>13324_13324_B0IiF_1_mTurqoise2-M_F_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCC NNNCNNNTAC NNGNAGANCA CCCCCATCGG CGANGGCCCC    61 GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCA AGCTGAGCAA AGACCCCAAC   121 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGGG TACCAAGGTG   181 TACGACCCCG AGCAGAGGAA GAGGATGATC ACCGGCCCCC AGTGGTGGGC CAGGTGCAAG   241 CAGATGAACG TGCTGGACAG CTTCATCAAC TACTACGACA GCGAGAAGCA CGCCGAGAAC   301 GCCGTGATCT TCCTGCACGG CAACGCCACT AGCAGCTACC TGTGGAGGCA CGTGGTGCCC   361 CACATCGAGC CCGTGGCCAG GTGCATCATC CCCGATCTGA TCGGCATGGG CAAGAGCGGC   421 AAGAGCGGCA ACGGCAGCTA CAGGCTGCTG GACCACTACA AGTACCTGAC CGCCTGGTTC   481 GAGCTTCTGA ACCTGCCCAA GAAGATCATC TTCGTGGGCC ACGACTGGGG CGCCGCCCTG   541 GCCTTCCACT ACGCCTACGA GCACCAGGAC AGGATCAAGG CCATCGTGCA CATGGAGAGC   601 GTGGTGGACG TGATCGAGAG CTGGGACGAG TGGCCAGACA TCGAGGAGGA CATCGCCCTG   661 ATCAAGANCG AGGAGGGCGA NAAGATGGTG CTGGAGANCA ACTTCNTTCG TGGAGACCGT   721 GCTGCCCAGC NANGATNATG AGAANNNCTG GANCCCCGAN GANTTCGNCC GCCCTACCTG   781 GANCCCNTTC NAGGNNNANN GNCNAGGNGN NNANNANNCN NNCNNNNNNC TGGGNCCNNN   841 ANANNATCCC CCNTGNNNNA AGGGCNGGNN ANGCCCCNNN NGTGNTGNCA ANANNNNNNG   901 NNNNAAANTA NNNNNNNCNN TANNNNNNNA NNCNNGNNNN NNANNCNNNG NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB13324_A5E8d.seq
>13324_13324_B0IiF_1_SV40pro_ori_F_F10_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG CCTCNNCCTC TGNNCTANTC NNGAAGTAGT GNGGANGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA   121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG   181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG   241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC   301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC   361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG   421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC   481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA   541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG   601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG   661 GCGAGGATCT CGTCGTGANC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG   721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACA   781 TAGCGTTGGC TACCCGNGAT ATTGCTGAAG ANCTTGNNGG CNAATGGGCT GANCGCTTCC   841 TCNNNNTTTA NNGTATCGCC NCTNCNNANN NGCANNGCAT CNNCNTTCTA TCNNCNNNCT 901 TGANNANNTN CNTNCTNANN NGGNNNNNTN GGGGNTNNNA AANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]

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