Yellow Nano-lantern to construct fusion proteins to the N-terminus.
|Clone info.||Cloning and expression vector to construct fusion proteins to the N-terminus of Yellow Nano-lantern.
Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. YNL was provided by Dr. Takeharu Nagai, Osaka University.
|Vector backbone||pEGFP-N1 (Plasmid)|
|Size of vector backbone||4.7 kb|
|Selectable markers||Kan^r (E. coli)|
|Depositor||Okada, Yasushi | Takai, Akira ||
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
|Terms and conditions set forth by the DEPOSITOR||In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.|
(Japanese text [open/close])
利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
|Catalog #||Resource name||Shipping form||Fee (non-profit org.)|
This clone will be sequenced a portion and digested by restriction enzyme for examination.
|Featured content||Nano-lantern (English text)|
|Featured content||Nano-lantern (Japanese text)|
|original||Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.|
|reference||Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.|