Please review the QC test results indicated by icon below as well as clone information before placing your order.

pYNL-N1 (#RDB13323)

Yellow Nano-lantern to construct fusion proteins to the N-terminus.

Clone info.	Cloning and expression vector to construct fusion proteins to the N-terminus of Yellow Nano-lantern.
Comment	Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. YNL was provided by Dr. Takeharu Nagai, Osaka University.
Vector backbone	pEGFP-N1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli)
Growth conditions	LB + Kan, 37C
Depositor|Developer	Okada, Yasushi | Takai, Akira |

 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13323	pYNL-N1	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pYNL-N1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13323).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13323_A5E8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB13323_A5E8a.seq
>13323_13323_B0IiF_2_CMV_Forward_KK_E04_05_ABI08.ab1     1 NNNNNNNNNN NNCNTTGNNN CNNATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT   121 CAAGCTTCGA ATTCTGCAGT CGACGGTACC GCGGGCCCGG GATCCACCGG TCGCCACCAT   181 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG   241 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG   301 CAAGCTGACC CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT   361 CGTGACCACC CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA   421 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT   481 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT   541 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA   601 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG   661 CATCAAGGCC AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA   721 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA   781 CCTGAGCTAC CNGTCCAAGC TGANCNNAGA CCCNNACGAG NNNCGCGATC ACATGGTCCT   841 GCTGGAGTTC GTGNNCGCCG CCNGGGGTAC CNAGGTGTAC GACCCNNAGC ANANGAAGAN   901 GANGATCANC GGNCCCCANN GGNTGGGNNN NGNNNNNNNC AGATGAANNG TGCTGGANAG   961 CTTCNNNAAC TACTACGNNA NCNAGNNNCN NGCCGAGNNC GNCNNNGNTN TTNNNGCNNN 1021 GNNNNNNNNN TAGCNNNNAN CNNNNNNNNG NNNNGNNNNN NNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB13323_A5E8b.seq
>13323_13323_B0IiF_2_SV40poryA_R2_KK_F04_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTGNATT NNTTTTATGT TTCAGGTTCA    61 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA   121 TTATGATCTA GAGTCGCGGC CGCCTACTGC TCGTTCTTCA GCACTCTCTC CACGAAGCTC   181 TTGATGTACT TGCCCATCTC GTCGGGGGCG TCCTCCTGGA GGAAGTGCAG GCCCTTCACC   241 TTCACGAACT CGGTGTTGGG GAACTTCTTG GCGCCCTCCA CGATGGCGTT GCTGAAGAAG   301 CCGGGGTCGC CCTCGATGAA CAGCTTGGGC AGGTCGTCGC TGGCTCTCAG GTAGGCGTTG   361 TAGTTTCTCA CGATCTGCAC CACGTCGGGC TTGCCGCCCT TCACCAGGGG GATCTCTCTG   421 GGCCAGCTCA GGGTGGGTCT TCTCACCTCG CCCTTCTCCT TGAAGGGCTC CAGGTAGGCG   481 GCGAACTCCT CGGGCTCCAG CTTTCTCATG ATCTTGCTGG GCAGCACGGT CTCCACGAAG   541 AAGTTGTTCT CCAGCACCAT CTTCTCGCCC TCCTCGCTCT TGATCAGGGC GATGTCCTCC   601 TCGATGTCTG GCCACTCGTC CCAGCTCTCG ATCACGTCCA CCACGCTCTC CATGTGCACG   661 ATGGCCTTGA TCCTGTCCTG GTGCTCGTAG GCGTAGTGGA AGGCCAGGGC GGCGCCCCNG   721 TCGTGGCCCA CGAAGATGAT CTTCTTGGGC AGGTTCANAA GCTCNNAACN NGGCGGTCAG   781 GTACTTGTAG TGGTCCAGCA GCCTGTAGCT GCCGTTGCCG CTCTTGCCGC TCTNGCCCAT   841 GCCGATCAGA TCNGGGNNGA TGCACCTGNC CACNGGGCTC GATGTGGGGC ANCNCGTGCC   901 TCCNNAGGTA GCTGCNNNTG NCGTTNNNGN GCANNAAGAT CACNGGNGTN NNNNGCGNGN   961 TTNNNNNTNN CGNNNNANNT GANNNANNNG NCNNGNNNNT NNATCTGNTT GNACCNNNGN 1021 CCCNNNNNNN GGGGNNNNGN NNNNNNNTNC NNNNNTNNNN NNNNNN //
Primer: mTurquoise2-M_F (Pr0577) Region: insert mid Sequence file: RDB13323_A5E8c.seq
>13323_13323_B0IiF_2_mTurquoise2-M_F_A10_01_ABI24.ab1     1 NNNNNNNNNN NCNNGNAGAA CACCCCCNTC GGCGACGGCC CCGTGCTGCT GCCCGACAAC    61 CACTACCTGA GCTACCAGTC CAAGCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG   121 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG GGTACCAAGG TGTACGACCC CGAGCAGAGG   181 AAGAGGATGA TCACCGGCCC CCAGTGGTGG GCCAGGTGCA AGCAGATGAA CGTGCTGGAC   241 AGCTTCATCA ACTACTACGA CAGCGAGAAG CACGCCGAGA ACGCCGTGAT CTTCCTGCAC   301 GGCAACGCCA CTAGCAGCTA CCTGTGGAGG CACGTGGTGC CCCACATCGA GCCCGTGGCC   361 AGGTGCATCA TCCCCGATCT GATCGGCATG GGCAAGAGCG GCAAGAGCGG CAACGGCAGC   421 TACAGGCTGC TGGACCACTA CAAGTACCTG ACCGCCTGGT TCGAGCTTCT GAACCTGCCC   481 AAGAAGATCA TCTTCGTGGG CCACGACTGG GGCGCCGCCC TGGCCTTCCA CTACGCCTAC   541 GAGCACCAGG ACAGGATCAA GGCCATCGTG CACATGGAGA GCGTGGTGGA CGTGATCGAG   601 AGCTGGGACG AGTGGCCAGA CATCGAGGAG GACATCGCCC TGNTCAAGAG CGAGGANGGC   661 GAGAAGATGG TGCTGGAGAA CAACTTCTTC GTGGANACCG TGCTGCCCAG CAAGATCATG   721 AGAAAGCTGG ANCCCGANGA NTTCGCCGCC TACCTGGNGC CCTTCNAGGA NAANGGCGAG   781 GTGAGAAGAN CCACCCNNNN NTGGCCCANA NAGATCCCCC NNNNGAAGGG CGGCAAGCCC   841 GACNNNGNGC ANATCGNGAN AAACTACNNN NCNNANNNNA NANNNNNCNN CNNNNNNCCN 901 NNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB13323_A5E8d.seq
>13323_13323_B0IiF_2_SV40pro_ori_F_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCCNCNNCCT CTNNNCTATT CNNGAAGTAG TGAGGAGGCT    61 TTTTTGGAGG NNNNNNCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC   121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC   181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA   241 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG   301 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG   361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG   421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG   481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC   541 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA   601 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC   661 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT   721 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCNNNAC   781 ATAGCGTTGG CTACNNGTGA TATTGCTGAA GANCTTGGCG GCGAATGGGC TGANCGCTTC   841 CTCGNGCTTT NCNGNNTCGC CGCTCCCNAN NNNCANCGCN NNCNCCTNCT ATCNNCNNTN 901 NTNGACNANN TCTTCTGANN NGGNNTNNTG GGGGNTNNGA ANNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]

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