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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pYNL-N1

Yellow Nano-lantern to construct fusion proteins to the N-terminus.

Catalog number RDB13323
Resource name pYNL-N1
Clone info. Cloning and expression vector to construct fusion proteins to the N-terminus of Yellow Nano-lantern.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. YNL was provided by Dr. Takeharu Nagai, Osaka University.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Depositor|Developer Okada, Yasushi | Takai, Akira |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
            付加的提供条件 利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13323 pYNL-N1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Nano-lantern (English text)
            Featured content Nano-lantern (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13323_A5E8p1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,insert 5'
            Sequence file: RDB13323_A5E8a.seq check
            >13323_13323_B0IiF_2_CMV_Forward_KK_E04_05_ABI08.ab1
                1 NNNNNNNNNN NNCNTTGNNN CNNATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
               61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT
              121 CAAGCTTCGA ATTCTGCAGT CGACGGTACC GCGGGCCCGG GATCCACCGG TCGCCACCAT
              181 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
              241 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
              301 CAAGCTGACC CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
              361 CGTGACCACC CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA
              421 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
              481 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
              541 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
              601 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG
              661 CATCAAGGCC AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA
              721 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
              781 CCTGAGCTAC CNGTCCAAGC TGANCNNAGA CCCNNACGAG NNNCGCGATC ACATGGTCCT
              841 GCTGGAGTTC GTGNNCGCCG CCNGGGGTAC CNAGGTGTAC GACCCNNAGC ANANGAAGAN
              901 GANGATCANC GGNCCCCANN GGNTGGGNNN NGNNNNNNNC AGATGAANNG TGCTGGANAG
              961 CTTCNNNAAC TACTACGNNA NCNAGNNNCN NGCCGAGNNC GNCNNNGNTN TTNNNGCNNN
            1021 GNNNNNNNNN TAGCNNNNAN CNNNNNNNNG NNNNGNNNNN NNNNN
            //
            Primer: SV40polyA_R2
            Region: insert 3'
            Sequence file: RDB13323_A5E8b.seq check
            >13323_13323_B0IiF_2_SV40poryA_R2_KK_F04_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTGNATT NNTTTTATGT TTCAGGTTCA
               61 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA
              121 TTATGATCTA GAGTCGCGGC CGCCTACTGC TCGTTCTTCA GCACTCTCTC CACGAAGCTC
              181 TTGATGTACT TGCCCATCTC GTCGGGGGCG TCCTCCTGGA GGAAGTGCAG GCCCTTCACC
              241 TTCACGAACT CGGTGTTGGG GAACTTCTTG GCGCCCTCCA CGATGGCGTT GCTGAAGAAG
              301 CCGGGGTCGC CCTCGATGAA CAGCTTGGGC AGGTCGTCGC TGGCTCTCAG GTAGGCGTTG
              361 TAGTTTCTCA CGATCTGCAC CACGTCGGGC TTGCCGCCCT TCACCAGGGG GATCTCTCTG
              421 GGCCAGCTCA GGGTGGGTCT TCTCACCTCG CCCTTCTCCT TGAAGGGCTC CAGGTAGGCG
              481 GCGAACTCCT CGGGCTCCAG CTTTCTCATG ATCTTGCTGG GCAGCACGGT CTCCACGAAG
              541 AAGTTGTTCT CCAGCACCAT CTTCTCGCCC TCCTCGCTCT TGATCAGGGC GATGTCCTCC
              601 TCGATGTCTG GCCACTCGTC CCAGCTCTCG ATCACGTCCA CCACGCTCTC CATGTGCACG
              661 ATGGCCTTGA TCCTGTCCTG GTGCTCGTAG GCGTAGTGGA AGGCCAGGGC GGCGCCCCNG
              721 TCGTGGCCCA CGAAGATGAT CTTCTTGGGC AGGTTCANAA GCTCNNAACN NGGCGGTCAG
              781 GTACTTGTAG TGGTCCAGCA GCCTGTAGCT GCCGTTGCCG CTCTTGCCGC TCTNGCCCAT
              841 GCCGATCAGA TCNGGGNNGA TGCACCTGNC CACNGGGCTC GATGTGGGGC ANCNCGTGCC
              901 TCCNNAGGTA GCTGCNNNTG NCGTTNNNGN GCANNAAGAT CACNGGNGTN NNNNGCGNGN
              961 TTNNNNNTNN CGNNNNANNT GANNNANNNG NCNNGNNNNT NNATCTGNTT GNACCNNNGN
            1021 CCCNNNNNNN GGGGNNNNGN NNNNNNNTNC NNNNNTNNNN NNNNNN
            //
            Primer: mTurquoise2-M_F
            Region: insert mid
            Sequence file: RDB13323_A5E8c.seq check
            >13323_13323_B0IiF_2_mTurquoise2-M_F_A10_01_ABI24.ab1
                1 NNNNNNNNNN NCNNGNAGAA CACCCCCNTC GGCGACGGCC CCGTGCTGCT GCCCGACAAC
               61 CACTACCTGA GCTACCAGTC CAAGCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG
              121 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG GGTACCAAGG TGTACGACCC CGAGCAGAGG
              181 AAGAGGATGA TCACCGGCCC CCAGTGGTGG GCCAGGTGCA AGCAGATGAA CGTGCTGGAC
              241 AGCTTCATCA ACTACTACGA CAGCGAGAAG CACGCCGAGA ACGCCGTGAT CTTCCTGCAC
              301 GGCAACGCCA CTAGCAGCTA CCTGTGGAGG CACGTGGTGC CCCACATCGA GCCCGTGGCC
              361 AGGTGCATCA TCCCCGATCT GATCGGCATG GGCAAGAGCG GCAAGAGCGG CAACGGCAGC
              421 TACAGGCTGC TGGACCACTA CAAGTACCTG ACCGCCTGGT TCGAGCTTCT GAACCTGCCC
              481 AAGAAGATCA TCTTCGTGGG CCACGACTGG GGCGCCGCCC TGGCCTTCCA CTACGCCTAC
              541 GAGCACCAGG ACAGGATCAA GGCCATCGTG CACATGGAGA GCGTGGTGGA CGTGATCGAG
              601 AGCTGGGACG AGTGGCCAGA CATCGAGGAG GACATCGCCC TGNTCAAGAG CGAGGANGGC
              661 GAGAAGATGG TGCTGGAGAA CAACTTCTTC GTGGANACCG TGCTGCCCAG CAAGATCATG
              721 AGAAAGCTGG ANCCCGANGA NTTCGCCGCC TACCTGGNGC CCTTCNAGGA NAANGGCGAG
              781 GTGAGAAGAN CCACCCNNNN NTGGCCCANA NAGATCCCCC NNNNGAAGGG CGGCAAGCCC
              841 GACNNNGNGC ANATCGNGAN AAACTACNNN NCNNANNNNA NANNNNNCNN CNNNNNNCCN
            901 NNNNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,NeoR_KanR
            Sequence file: RDB13323_A5E8d.seq check
            >13323_13323_B0IiF_2_SV40pro_ori_F_D03_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCCNCNNCCT CTNNNCTATT CNNGAAGTAG TGAGGAGGCT
               61 TTTTTGGAGG NNNNNNCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC
              121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
              181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
              241 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
              301 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
              361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
              421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
              481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
              541 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA
              601 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC
              661 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
              721 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCNNNAC
              781 ATAGCGTTGG CTACNNGTGA TATTGCTGAA GANCTTGGCG GCGAATGGGC TGANCGCTTC
              841 CTCGNGCTTT NCNGNNTCGC CGCTCCCNAN NNNCANCGCN NNCNCCTNCT ATCNNCNNTN
            901 NTNGACNANN TCTTCTGANN NGGNNTNNTG GGGGNTNNGA ANNNNNNNNN N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
            reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl