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pUC-T7-HCV HA-2A(silent-1)2B-HA

HCV IRES-dependent expression vector of 2A(silent-1)2B

Catalog number RDB13263
Resource name pUC-T7-HCV HA-2A(silent-1)2B-HA
Clone info. HCV IRES-dependent expression vector of 2A(silent-1)2B.
Encephalomyocarditis virus (EMCV), GenBankAcc. No: M81861. Expression was confirmed by depositor with Western blot.
Vector backbone pUC-T7-HCV-HA (Plasmid)
Size of vector backbone 3.4 kb
Selectable markers Amp^r
Gene/insert name encephalomyocarditis virus 2A, 2B cDNA
Depositor Imataka, Hiroaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13263 pUC-T7-HCV HA-2A(silent-1)2B-HA Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.
reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.

2019.07.20

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