HCV IRES-dependent expression vector of 2A-2B
Catalog number | RDB13262 |
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Resource name | pUC-T7-HCV HA-2A-2B-HA |
Clone info. | HCV IRES-dependent expression vector of 2A-2B. Encephalomyocarditis virus (EMCV), GenBankAcc. No: M81861. Expression was confirmed by depositor with Western blot. |
Vector backbone | pUC-T7-HCV-HA (Plasmid) |
Size of vector backbone | 3.4 kb |
Selectable markers | Amp^r |
Gene/insert name | encephalomyocarditis virus 2A, 2B cDNA |
Depositor | Imataka, Hiroaki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014) |
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Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB13262 | pUC-T7-HCV HA-2A-2B-HA | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
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This clone will be sequenced a portion and digested by restriction enzyme for examination.
Original, user report and related articles
original | Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. |
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reference | Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838. |
2019.07.20
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