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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

p3xFLAG-CMV-14_mGBA1 (#RDB13236)

Expression plasmid of mouse GBA1

Clone info. Expression plasmid of mouse GBA1. GBA1 was cloned from genomic DNA of mouse kidney.
Comment Expression was confirmed by the depositor with anti-FLAG M2 antibody.
Vector backbone p3xFLAG-CMV-14 (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Mouse GBA1 cDNA
Depositor|Developer Murofushi, Kimiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 441 (4), 838-843, 2013)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biochem. Biophys. Res. Commun., 441 (4), 838-843, 2013)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13236 p3xFLAG-CMV-14_mGBA1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV-14_mGBA1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13236).

Reference section:

Akiyama, H., Kobayashi, S., Hirabayashi, Y., Murakami-Murofushi, K., Cholesterol glucosylation is catalyzed by transglucosylation reaction of beta-glucosidase 1. Biochem. Biophys. Res. Commun. 441 (4): 838-843 (2013). PMID 24211208. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13236_B3Aup1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'
Sequence file: RDB13236_B3Aua.seq check
>13236_13236_B3Au_2_CMV_Forward_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNGCNNAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCGTTTAG TGAACCGTCA GAATTAAGCT TGCGGCCGCG AATTCGCCAC
  121 CATGGCTGCC AGGCTCATCG GATTCTTCCT ATTTCAGGCG GTATCTTGGG CATATGGTGC
  181 CCAACCCTGC ATCCCCAAAA GCTTTGGCTA CAGCTCAGTG GTCTGTGTCT GTAATGCATC
  241 GTACTGTGAC TCTCTTGACC CCGTGACCTT ACCGGCTCTG GGTACCTTCA GCCGTTACGA
  301 GAGCACTCGA CGTGGACGTC GGATGGAGCT GAGTGTCGGG GCCATCCAGG CCAATCGCAC
  361 TGGCACAGGG TTACTACTCA CTTTGCAGCC AGAAAAGAAG TTCCAGAAAG TGAAAGGATT
  421 TGGAGGCGCC ATGACAGACG CCACTGCGCT CAACATCCTT GCTTTGTCCC CACCTACTCA
  481 GAAGCTGCTA CTCAGATCCT ACTTCTCTAC CAACGGAATT GAATATAACA TCATCCGGGT
  541 ACCCATGGCC AGTTGTGACT TCTCCATCCG TGTCTATACC TATGCTGACA CCCCTAACGA
  601 CTTCCAGTTA TCCAACTTCA GCCTCCCAGA AGAAGACACC AAGCTCAAGA TACCCCTGAT
  661 TCACCAAGCC CTGAAGATGT CCTCACGCCC CATTTCACTC TTTGCCAGTC CCTGGACATC
  721 ACCCACTTGG CTCAAGACCA ATGGAAGAGT GAATGGGAAG GGGTCGCTCA AGGGTCAGCC
  781 AGGGGATATC TTTCACCAGA CCTGGGCCAA TTACTTTGTC AAGTTTCTGG ATGCTTATGC
  841 TAAGTATGGC CTAAGATTCT GGGCAGTGAC AGCGGANANT GAACCTACAG CANGGCTCTN
  901 CACGGGNNAN CCNTTCCAGT GNCNGGGCTT CACTNCNGAA CACCANANAN NNTTCATTTC
961 CCGNGANCNN NGNNNAGCCC NTNNNCNNAC NNNTNNCCNN GNNGNNNNNN NNNN
//
Primer: CMV24ver2 (Pr0018)
Region: hGH pA,3xFLAG,insert 3'
Sequence file: RDB13236_B3Aub.seq check
>13236_13236_B3Au_2_CMV24ver2_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNNNAACTTC NNGGGCCAGG ANNGGCNNTG GGGAGGGGTC
   61 ACAGGGATGC CACCCGGGAT CACTACTTGT CATCGTCATC CTTGTAGTCG ATGTCATGAT
  121 CTTTATAATC ACCGTCATGG TCTTTGTAGT CAGCCCGGGA TCCTCTAGAC TGGCGACGCC
  181 ACAGGTAAGT GTGAATGGAG TAGCCAGGTG ACACGGTCTC CAGGAAGCCC AGGTCAGGAT
  241 CACTGATGGT AAGAGGGACA TCCTCCGAAG ATCGGTTTAA CACGACCACA ACTGCAGAGC
  301 CGTCAGGGCG TAACAGTGCT ACTGTTTCCA AGTCAGTGCT CTCACTGGCC ACCAACGCCA
  361 CTCTCTGGGA TCCCTCGGGA ATGAACTTGC TGAAGTGGCC AAGATGGTAG AACATGGGCT
  421 GTTTGTAAAA TGCGTCTTTG GGGATGTCGA CAATAATGGG GCTATCGACA AAGTTGCGGA
  481 CCCAGTTGGG CCCTCCTTCA GGATTCAGGG CAAGGTTCCA GTCCGTCCAT CCAGTTACGT
  541 GGTAAAGGAG GTTCGTAATG ATGCTGTGAC TGTACTGCAT CCCTCGATCC CAGGAGCCCA
  601 ACCGAACACT CTGTTCCCAG AACTTGGAGC CCACGCAGGC CTCCGAAGCA AAGAGCATTG
  661 TGTTGGGGAA CAAGCGGTGT GTCTCTCCTA AAGTAGCTTT GGCTGGAGCC AGGAAATCCA
  721 TATACCAGTG AACAGCAATG CCATGAACGT ACTTAGCTGC CTCTGGATCA GAGAGCACCA
  781 CCTCTGCCCA GCGGGGTAGC AGCAAGCGTT GGTCATCTAG CATGAGTAGC TTCACATCAT
  841 GGGAACTGTT GGCAAGGGCT GGCNNTANGT CACGGGAAAT GAANTCTCTC TGGTGNTCNG
  901 GANTGAAGCC NANGNACNGN NANNGGTANC CCGNGNAGAN CCCTGCTGNN NNTNCATTCN
  961 CCNCTNTCNC TGNCCANANN CNNNNNNNAT ACNTNANCAT NNNNNNNCNN AANNNNGNNN
1021 ANNNNNNNNG NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: NeoR
Sequence file: RDB13236_B3Auc.seq check
>13236_13236_B3Au_2_SV40pro_ori_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CCGCCTCNGC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG
   61 CTTTTTTGGA GGANTTGATC AGCTTGGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC
  121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
  181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
  241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
  301 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
  361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
  421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
  481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
  541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
  601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG
  661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
  721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG NCCGGCTGGG TGTGGCGGAC CGCTATCAGG
  781 ACATAGCGTT GGCTACCCGT GATATTGCTG AANANCTTGN NGGCGAATGG GCTGACCGCT
  841 TCCTCGNGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGNNTTC TATCGCCTTC
  901 TTGACGANTT CTTCTGANCG GGNNTCTGGG GTNNGAANTG NNCGANCAAN CGACNCCCNN
961 NNTGCCATCN CGAGANTTCN ATTCCNNNNN NNNNTTCTAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Akiyama, H., Cholesterol glucosylation is catalyzed by transglucosylation reaction of beta-glucosidase 1. Biochem. Biophys. Res. Commun. 441 (4): 838-843 (2013). PMID 24211208. [link to RRC of NBRP]
reference Carstea, E.D., Molecular and functional characterization of the murine glucocerebrosidase gene. Biochem. Biophys. Res. Commun. 184 (3): 1477-1483 (1992). PMID 1317175.