AAV vector of FLAG-tTA under the control of Substance P promoter
Alternative name | V-S-tTA |
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Clone info. | AAV vector of FLAG-tTA under the control of Substance P promoter. |
Vector backbone | pAAV (Plasmid) |
Gene/insert name | Mouse Substance P promoter genomic DNA |
Depositor|Developer | Nakanishi, Shigetada | |
Sequence (full) | RDB13212zzt01.seq compiled by us |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Neuron 66: 896-907, 2010) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Neuron 66: 896-907, 2010)。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13212 | pAAV-SP-FtTA | DNA solution |
Materials & Methods section:
The pAAV-SP-FtTA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13212). |
Reference section:
Hikida, T., Kimura, K., Wada, N., Funabiki, K., Nakanishi, S., Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior. Neuron 66 (6): 896-907 (2010). PMID 20620875. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13212_A5Cip1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pAAV-MCS_F1 (Pr0766) Region: SP pro 5' Sequence file: RDB13212_A5Cia.seq |
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>D02088F2_A5Ci_1_pAAV-HCS_F1_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTG TGNTGGCCCN TCACTTTGGC AAAGAATTGG 61 GANTCGAACA TCGATCATAA GCGGAGGCAT GAAATTTTCA AATTGTTTAG GCAAATGACT 121 GTACATTTGT GTTCACCATT TCTCCTTTTA AAAGTTTTGA AAATAAAAAT AGAATGTTCA 181 CATTATGAAA TCCCTCTGAA TGTCTACATT TAGGTTTTAA AACAATATAA ACACTGGAGG 241 CAAACATTAG TTTGGCTTGC AAACACTTCT TTCCTCTTCA TATTATACTA AAAACATATT 301 AGGGAACGAT GATTCCCAAA AGAAAACAAC TCTATCCAAA AGACCCCATC GCTCTGGGTG 361 TGGTGGTACA CACCTGTAGT TCCAGTAACA AGTAGGCTGA GTTAGGAGAA TACCCATTTT 421 GAGTCCTAGT CTAAAAAAGT CAAAACAAAT AACTGCAACA TCGTTTACTT CATCCAAAGA 481 TGGAAAAGTA TTATACATCT GGGACAGAAG GTATCTGTAA AACCGTGGTT TGCTAAAGAC 541 TTCTGTCTCT GACAGATCTG CAGCACACAC ACACACACAC ACACACACCC TTGGTGACAA 601 AGGTAAACAT ACAGACTTTA TAGCCCTCCA ACAAGGCTTA AGGGGAGAGA GGCACAATTA 661 TTCTCTTGCA ACTCCCTCTA CCTTCTGTGT GTGACTGCGA AGAANCTTCA GAGGAACGAA 721 AGANGTCCTC AGATGGCTGG TGGGTACGTT CCACATGCTG TTCTACATGC TACAGCGTCC 781 TTGAATGGCT GGTGGGTAAG TTCTACATGC TACAGCGTCC TCAGATGGCT GGTGGGTAAG 841 TTCTACATGC TACAGCGTCC TCGGATGGNT GGNGGGGTAN GTTNNNCATG CTGTTCTACN 901 TGCTACAGCA GGCTTCNATT CNNCTTTCTN NNAAATGNAA AGCAAGNCTG CTTTNCTNNN 961 CTANNANANN AGANTGAAAC CCCTTTNNNA NNGNTAGANA AANGANANAN ANTATTCTGG 1021 NNNNNTNNNG NANGNNNGNC NNNNNNNNNN // |
Primer: hGHpolyA-R (Pr0350) Region: hGH p(A), tTA 3' Sequence file: RDB13212_A5Cib.seq |
>D02088F2_A5Ci_1_hGHpolyA-R_C11_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNCN NNNNNANTAN AGAAGGNNAC CTAGTCNGAC AAAATGATGC 61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA 121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCGTAGAT CTCTCGAGCT ACCCACCGTA 181 CTCGTCAATT CCAAGGGCAT CGGTAAACAT CTGCTCAAAC TCGAAGTCGG CCATATCCAG 241 AGCGCCGTAG GGGGCGGAGT CGTGGGGGGT AAATCCCGGA CCCGGGGAAT CCCCGTCCCC 301 CAACATGTCC AGATCGAAAT CGTCTAGCGC GTCGGCATGC GCCATCGCCA CGTCCTCGCC 361 GTCTAAGTGG AGCTCGTCCC CCAGGCTGAC ATCGGTCGGG GGGGCCGTCG ACAGTCTGCG 421 CGTGTGTCCC GCGGGGAGAA AGGACAGGCG CGGAGCCGCC AGCCCCGCCT CTTCGGGGGC 481 GTCGTCGTCC GGGAGATCGA GCAGGCCCTC GATGGTAGAC CCGTAATTGT TTTTCGTACG 541 CGCGCGGCTG TACGCGGACC CACTTTCACA TTTAAGTTGT TTTTCTAATC CGCATATGAT 601 CAATTCAAGG CCGAATAAGA AGGCTGGCTC TGCACCTTGG TGATCAAATA ATTCGATAGC 661 TTGTCGTAAT AATGGCGGCA TACTATCAGT AGTAGGTGTT TCCCTTTCTT CTTTAGCGAC 721 TTGATGCTCT TGATCTTCCN ATACGCAACC TAAAGTAAAA TGCCCCACAG CGCTGANTGC 781 ATATAATGCA TTCTCTAGTG AAAAANCTTG TTGGNATAAA AAGGNTAATT GATTTTCGAG 841 ANTTTCATAC TGNTTTTTCT GTANGNCGNG TANCNAAATG NACTTTTTGC TCCNTCGCGA 901 TGANTTANTA AANCANATNT AAAACTTTTA NCGTNNNTNN NNAAAAAANT CNNGNCNGCT 961 TNCCNNNNNT NNNGNNAAAA NNGANTATGN NNNNNNNNN // |
Primer: TetR-N_R (Pr0764) Region: tTA 5', FLAG, SP pro 3' Sequence file: RDB13212_A5Cic.seq |
>D02088F2_A5Ci_1_TetR-N_R_B11_05_ABI24.ab1 1 NNNNNNNNNN TNNNNNNTGC NNNTACNACG TAGGCTGCTC TACNCCTAGC TTCTGGGCGA 61 GTTTACGGGT TGTTAAACCT TCGATTCCGA CCTCATTAAG CAGCTCTAAT GCGCTGTTAA 121 TCACTTTACT TTTATCTAAT CTAGATGGAT CCTTGTCATC GTCGTCCTTG TAGTCGAGAT 181 CCCCCGTCTC TTGTTCCATG GTGGCGGCCG CCAGTGTGAT GGATATCTGC AGAATTCGCC 241 CTTAGCGCTA GCGTTCGATT TCTGAAGAGA GTAGGAGACA GAGGAAGGTG GGAAAGAGAC 301 ATTATACATA AGGAGGCTGT GGCCACCCAA GAGTTAGAGC AGCTGAATTT CAGTGCCACA 361 AGGAAGAAAG AACAGGTCCC GCTAAACCAA GATTAAAATA AAAACAAAAC CAAAAGCCCA 421 CCAGGTCGAA CACTTCTCCA AACCCTTTAA GAAGGCTCCT GAGGCGCCAA AGAGCCTCAA 481 ATCCTCCTCT CCTTGAGTCT CAGCGTCCCA GCCCCCTACC CACTGGCCCA CACTCTCAAG 541 CCTGAAGACT CTGCAGGTGG CAGACAAAGA GAGGCGTTGG TATTTGCACA CTTTCTCTCC 601 GGGTCTGAGA ATGGAGTGGG AGCAGAGCCA GGGCGGACGC CACCTCCGAC GAAGGCACGA 661 CGGGTCCCAG GCAGCCTTGG CTGGCAGGCT AGAGTGGTAC TCACTGCGGT CAGACCGCGG 721 GGNTGCTCCG CAGGTGCGCA CTTGCTGGGC GCTCTTTGCT CTCCTTGGCC GCGGTGCANG 781 ANCGGANCAN GTCCGANTGC GGTCTANTCC CCAGCTCCTG CTTTGCGGTA TTTATCTCAN 841 GCGTGACGAG CCTGTAGAGC CACGNNNCAC TCTCCCNACG CNACTTTCTG CTCNATANTT 901 NATTAGCCGC CCCCTCGCCT TNCGCTNNNG NNANGNANAN TTTNNNNNNN NGNNANNNNN 961 CNNNNNNCAT NNNNNNNNNN // |
Primer: CMV_Forward (Pr0016) Region: CMV pro, SP pro 5' Sequence file: RDB13212_A5Cid.seq |
>D02088F2_A5Ci_1_CMV_Forward_A11_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT 121 CCATAGAAGA CACCGGGACC GATCCAGCCT CCGCGGATTC GAATCCCGGC CGGGAACGGT 181 GCATTGGAAC GCGGATTCCC CGTGCCAAGA GTGACGTAAG TACCGCCTAT AGAGTCTATA 241 GGCCCACAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT GTTTATCTTA TTTCTAATAC 301 TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT GTATCATGCC TCTTTGCACC 361 ATTCTAAAGA ATAACAGTGA TAATTTCTGG GTTAAGGCAA TAGCAATATT TCTGCATATA 421 AATATTTCTG CATATAAATT GTAACTGATG TAAGAGGTTT CATATTGCTA ATAGCAGCTA 481 CAATCCAGCT ACCATTCTGC TTTTATTTTA TGGTTGGGAT AAGGCTGGAT TATTCTGAGT 541 CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA TCTTCCTCCC ACAGCTCCTG 601 GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA AAGAATTGGG ATTCGAACAT 661 CGATCATAAG CGGAGGCATG AAATTTTCAA ATTGTTTAGG CAAATGACTG TACATTTGTG 721 TTCACCATTT CTCCTTTTAA AAGTTTTGAA AATAAAAATA GAATGTTCAC ATTATGAAAT 781 CCCTCTGAAT GTCTACATTT ANGTTTTAAA ACAATATAAA CACTGGANGC AAACATTANT 841 TTGGCTTGCA AACACTTCTT TCCTCTTCAT ATTATACTAN NNNCATATTN NNNAANGATG 901 ANNNCCAAAA GAAAANAACT CTATCCAAAA GANCCNATCG CTCTGGGNNN GGNGGNANNC 961 ANCNNNNNNN CCANNNANNN NNNNNNTGAN TNANNANAAN NNNNNNTTTN NNNNNNNNN // |
Primer: ColE1ori_F (Pr0393) Region: ori, AAV2 ITR Sequence file: RDB13212_A5Cie.seq |
>D02088F2_A5Ci_1_ColE1ori_F_D11_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNTCTG NNTTGANCGN CGANTTTTGT GANGCTCGTC 61 AGGGGGGCGG ANCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 121 TTGCTGGCCT TTTGCTCACA TGTCCTGCAG GCAGCTGCGC GCTCNNTCNN TCNNNGANGN 181 CNNNNNNNNN N // |
Primer: f1ori_F (Pr0280) Region: f1 ori, AAV2 ITR Sequence file: RDB13212_A5Cif.seq |
>D02088F2_A5Ci_1_f1ori_F_E11_14_ABI24.ab1 1 NNNNNNNNNN NNNNNGNANT GNAGCGGTCN CGCTGCGCGT AACCNCCACA CCCGCCGCGC 61 TTAATGCGCC GCTACAGGGC GCGTACTATG GTTGCTTTGA CGTATGCGGT GTGAAATACC 121 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCCC TGCAGGCAGC TGCGCGCTC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Hikida, T., Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior. Neuron 66 (6): 896-907 (2010). PMID 20620875. [link to RRC of NBRP] |
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