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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV-SP-FtTA (#RDB13212)

AAV vector of FLAG-tTA under the control of Substance P promoter

Alternative name V-S-tTA
Clone info. AAV vector of FLAG-tTA under the control of Substance P promoter.
Vector backbone pAAV (Plasmid)
Gene/insert name Mouse Substance P promoter genomic DNA
Depositor|Developer Nakanishi, Shigetada |
 
Sequence (full) RDB13212zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Neuron 66: 896-907, 2010) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Neuron 66: 896-907, 2010)。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

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RDB13212 pAAV-SP-FtTA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAAV-SP-FtTA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13212).

Reference section:

Hikida, T., Kimura, K., Wada, N., Funabiki, K., Nakanishi, S., Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior. Neuron 66 (6): 896-907 (2010). PMID 20620875. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13212_A5Cip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAAV-MCS_F1 (Pr0766)
Region: SP pro 5'
Sequence file: RDB13212_A5Cia.seq check
>D02088F2_A5Ci_1_pAAV-HCS_F1_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTG TGNTGGCCCN TCACTTTGGC AAAGAATTGG
   61 GANTCGAACA TCGATCATAA GCGGAGGCAT GAAATTTTCA AATTGTTTAG GCAAATGACT
  121 GTACATTTGT GTTCACCATT TCTCCTTTTA AAAGTTTTGA AAATAAAAAT AGAATGTTCA
  181 CATTATGAAA TCCCTCTGAA TGTCTACATT TAGGTTTTAA AACAATATAA ACACTGGAGG
  241 CAAACATTAG TTTGGCTTGC AAACACTTCT TTCCTCTTCA TATTATACTA AAAACATATT
  301 AGGGAACGAT GATTCCCAAA AGAAAACAAC TCTATCCAAA AGACCCCATC GCTCTGGGTG
  361 TGGTGGTACA CACCTGTAGT TCCAGTAACA AGTAGGCTGA GTTAGGAGAA TACCCATTTT
  421 GAGTCCTAGT CTAAAAAAGT CAAAACAAAT AACTGCAACA TCGTTTACTT CATCCAAAGA
  481 TGGAAAAGTA TTATACATCT GGGACAGAAG GTATCTGTAA AACCGTGGTT TGCTAAAGAC
  541 TTCTGTCTCT GACAGATCTG CAGCACACAC ACACACACAC ACACACACCC TTGGTGACAA
  601 AGGTAAACAT ACAGACTTTA TAGCCCTCCA ACAAGGCTTA AGGGGAGAGA GGCACAATTA
  661 TTCTCTTGCA ACTCCCTCTA CCTTCTGTGT GTGACTGCGA AGAANCTTCA GAGGAACGAA
  721 AGANGTCCTC AGATGGCTGG TGGGTACGTT CCACATGCTG TTCTACATGC TACAGCGTCC
  781 TTGAATGGCT GGTGGGTAAG TTCTACATGC TACAGCGTCC TCAGATGGCT GGTGGGTAAG
  841 TTCTACATGC TACAGCGTCC TCGGATGGNT GGNGGGGTAN GTTNNNCATG CTGTTCTACN
  901 TGCTACAGCA GGCTTCNATT CNNCTTTCTN NNAAATGNAA AGCAAGNCTG CTTTNCTNNN
  961 CTANNANANN AGANTGAAAC CCCTTTNNNA NNGNTAGANA AANGANANAN ANTATTCTGG
1021 NNNNNTNNNG NANGNNNGNC NNNNNNNNNN
//
Primer: hGHpolyA-R (Pr0350)
Region: hGH p(A), tTA 3'
Sequence file: RDB13212_A5Cib.seq check
>D02088F2_A5Ci_1_hGHpolyA-R_C11_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCN NNNNNANTAN AGAAGGNNAC CTAGTCNGAC AAAATGATGC
   61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA
  121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCGTAGAT CTCTCGAGCT ACCCACCGTA
  181 CTCGTCAATT CCAAGGGCAT CGGTAAACAT CTGCTCAAAC TCGAAGTCGG CCATATCCAG
  241 AGCGCCGTAG GGGGCGGAGT CGTGGGGGGT AAATCCCGGA CCCGGGGAAT CCCCGTCCCC
  301 CAACATGTCC AGATCGAAAT CGTCTAGCGC GTCGGCATGC GCCATCGCCA CGTCCTCGCC
  361 GTCTAAGTGG AGCTCGTCCC CCAGGCTGAC ATCGGTCGGG GGGGCCGTCG ACAGTCTGCG
  421 CGTGTGTCCC GCGGGGAGAA AGGACAGGCG CGGAGCCGCC AGCCCCGCCT CTTCGGGGGC
  481 GTCGTCGTCC GGGAGATCGA GCAGGCCCTC GATGGTAGAC CCGTAATTGT TTTTCGTACG
  541 CGCGCGGCTG TACGCGGACC CACTTTCACA TTTAAGTTGT TTTTCTAATC CGCATATGAT
  601 CAATTCAAGG CCGAATAAGA AGGCTGGCTC TGCACCTTGG TGATCAAATA ATTCGATAGC
  661 TTGTCGTAAT AATGGCGGCA TACTATCAGT AGTAGGTGTT TCCCTTTCTT CTTTAGCGAC
  721 TTGATGCTCT TGATCTTCCN ATACGCAACC TAAAGTAAAA TGCCCCACAG CGCTGANTGC
  781 ATATAATGCA TTCTCTAGTG AAAAANCTTG TTGGNATAAA AAGGNTAATT GATTTTCGAG
  841 ANTTTCATAC TGNTTTTTCT GTANGNCGNG TANCNAAATG NACTTTTTGC TCCNTCGCGA
  901 TGANTTANTA AANCANATNT AAAACTTTTA NCGTNNNTNN NNAAAAAANT CNNGNCNGCT
961 TNCCNNNNNT NNNGNNAAAA NNGANTATGN NNNNNNNNN
//
Primer: TetR-N_R (Pr0764)
Region: tTA 5', FLAG, SP pro 3'
Sequence file: RDB13212_A5Cic.seq check
>D02088F2_A5Ci_1_TetR-N_R_B11_05_ABI24.ab1
    1 NNNNNNNNNN TNNNNNNTGC NNNTACNACG TAGGCTGCTC TACNCCTAGC TTCTGGGCGA
   61 GTTTACGGGT TGTTAAACCT TCGATTCCGA CCTCATTAAG CAGCTCTAAT GCGCTGTTAA
  121 TCACTTTACT TTTATCTAAT CTAGATGGAT CCTTGTCATC GTCGTCCTTG TAGTCGAGAT
  181 CCCCCGTCTC TTGTTCCATG GTGGCGGCCG CCAGTGTGAT GGATATCTGC AGAATTCGCC
  241 CTTAGCGCTA GCGTTCGATT TCTGAAGAGA GTAGGAGACA GAGGAAGGTG GGAAAGAGAC
  301 ATTATACATA AGGAGGCTGT GGCCACCCAA GAGTTAGAGC AGCTGAATTT CAGTGCCACA
  361 AGGAAGAAAG AACAGGTCCC GCTAAACCAA GATTAAAATA AAAACAAAAC CAAAAGCCCA
  421 CCAGGTCGAA CACTTCTCCA AACCCTTTAA GAAGGCTCCT GAGGCGCCAA AGAGCCTCAA
  481 ATCCTCCTCT CCTTGAGTCT CAGCGTCCCA GCCCCCTACC CACTGGCCCA CACTCTCAAG
  541 CCTGAAGACT CTGCAGGTGG CAGACAAAGA GAGGCGTTGG TATTTGCACA CTTTCTCTCC
  601 GGGTCTGAGA ATGGAGTGGG AGCAGAGCCA GGGCGGACGC CACCTCCGAC GAAGGCACGA
  661 CGGGTCCCAG GCAGCCTTGG CTGGCAGGCT AGAGTGGTAC TCACTGCGGT CAGACCGCGG
  721 GGNTGCTCCG CAGGTGCGCA CTTGCTGGGC GCTCTTTGCT CTCCTTGGCC GCGGTGCANG
  781 ANCGGANCAN GTCCGANTGC GGTCTANTCC CCAGCTCCTG CTTTGCGGTA TTTATCTCAN
  841 GCGTGACGAG CCTGTAGAGC CACGNNNCAC TCTCCCNACG CNACTTTCTG CTCNATANTT
  901 NATTAGCCGC CCCCTCGCCT TNCGCTNNNG NNANGNANAN TTTNNNNNNN NGNNANNNNN
961 CNNNNNNCAT NNNNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, SP pro 5'
Sequence file: RDB13212_A5Cid.seq check
>D02088F2_A5Ci_1_CMV_Forward_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT
  121 CCATAGAAGA CACCGGGACC GATCCAGCCT CCGCGGATTC GAATCCCGGC CGGGAACGGT
  181 GCATTGGAAC GCGGATTCCC CGTGCCAAGA GTGACGTAAG TACCGCCTAT AGAGTCTATA
  241 GGCCCACAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT GTTTATCTTA TTTCTAATAC
  301 TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT GTATCATGCC TCTTTGCACC
  361 ATTCTAAAGA ATAACAGTGA TAATTTCTGG GTTAAGGCAA TAGCAATATT TCTGCATATA
  421 AATATTTCTG CATATAAATT GTAACTGATG TAAGAGGTTT CATATTGCTA ATAGCAGCTA
  481 CAATCCAGCT ACCATTCTGC TTTTATTTTA TGGTTGGGAT AAGGCTGGAT TATTCTGAGT
  541 CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA TCTTCCTCCC ACAGCTCCTG
  601 GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA AAGAATTGGG ATTCGAACAT
  661 CGATCATAAG CGGAGGCATG AAATTTTCAA ATTGTTTAGG CAAATGACTG TACATTTGTG
  721 TTCACCATTT CTCCTTTTAA AAGTTTTGAA AATAAAAATA GAATGTTCAC ATTATGAAAT
  781 CCCTCTGAAT GTCTACATTT ANGTTTTAAA ACAATATAAA CACTGGANGC AAACATTANT
  841 TTGGCTTGCA AACACTTCTT TCCTCTTCAT ATTATACTAN NNNCATATTN NNNAANGATG
  901 ANNNCCAAAA GAAAANAACT CTATCCAAAA GANCCNATCG CTCTGGGNNN GGNGGNANNC
961 ANCNNNNNNN CCANNNANNN NNNNNNTGAN TNANNANAAN NNNNNNTTTN NNNNNNNNN
//
Primer: ColE1ori_F (Pr0393)
Region: ori, AAV2 ITR
Sequence file: RDB13212_A5Cie.seq check
>D02088F2_A5Ci_1_ColE1ori_F_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNTCTG NNTTGANCGN CGANTTTTGT GANGCTCGTC
   61 AGGGGGGCGG ANCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
  121 TTGCTGGCCT TTTGCTCACA TGTCCTGCAG GCAGCTGCGC GCTCNNTCNN TCNNNGANGN
181 CNNNNNNNNN N
//
Primer: f1ori_F (Pr0280)
Region: f1 ori, AAV2 ITR
Sequence file: RDB13212_A5Cif.seq check
>D02088F2_A5Ci_1_f1ori_F_E11_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNANT GNAGCGGTCN CGCTGCGCGT AACCNCCACA CCCGCCGCGC
   61 TTAATGCGCC GCTACAGGGC GCGTACTATG GTTGCTTTGA CGTATGCGGT GTGAAATACC
121 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCCC TGCAGGCAGC TGCGCGCTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hikida, T., Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior. Neuron 66 (6): 896-907 (2010). PMID 20620875. [link to RRC of NBRP]