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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV-Enk-FtTA (#RDB13211)

AAV vector of FLAG-tTA under the control of Enkephalin promoter

Alternative name V-E-tTA
Clone info. AAV vector of FLAG-tTA under the control of Enkephalin promoter.
Vector backbone pAAV (Plasmid)
Gene/insert name Mouse Enkephalin promoter genomic DNA
Depositor|Developer Nakanishi, Shigetada |
 
Sequence (full) RDB13211zzt01.seq compiled by us

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Neuron 66: 896-907, 2010) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Neuron 66: 896-907, 2010)。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

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RDB13211 pAAV-Enk-FtTA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAAV-Enk-FtTA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13211).

Reference section:

Hikida, T., Kimura, K., Wada, N., Funabiki, K., Nakanishi, S., Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior. Neuron 66 (6): 896-907 (2010). PMID 20620875. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13211_A5Cip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAAV-MCS_F1 (Pr0766)
Region: Enk pro 5'
Sequence file: RDB13211_A5Cia.seq check
>D02088F1_A5Ci_1_pAAV-HCS_F1_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTCTGTG TGNTGGCCCN TCACTTTGGC AAAGAATTGG
   61 GANTCGAACA TCGATTAGCG ACCAAAATGG GAGACGCACT TCAGATTGCG TCTCTCAGGA
  121 GCTGCGTGAA GGCAGGAACT GCAAAGATTG AAAAGGGTGG AGGATCCCTT GCAAATGTCT
  181 GGGAAAGCCT TGAGACCCTC TCTTTGTTGT AGCCTATTTG GACTAGGAAG ATAGGTACAC
  241 ACAATGGGTA AATCTTTGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG
  301 AGAGAGAAGA TAACTGGTAA CAGATCCAAA GGAGAGGTGG TTTAAAACAG CTTTTGAGGC
  361 CAGACGGGTT AAATGGTTTC GTTCATTATT CCAGATGATT CTGTCCTAAA CACTGTGCCT
  421 CCTCTCTCGG GTACTGACAT AAAATAGCAT GTTACCTCGG CTGCTGTTCT ACAAATAGGA
  481 GAAAAAAAAA AGATGAGCGC TTGGAGTCTA ACTAAGGGTT AGACGATCTA ATCTAAGCTT
  541 CCTGAGTGGC ACCTATCTAC AGACCCTGGA AGGGAGGGAG TGGGCTGCTC TAAAAGCCCC
  601 AGCCTCACCT TTTGGAGCCA GGCCTCTCCT GGAGGACAAG ATGTGCAAGA CCTAAGCCAC
  661 TTGTGTCCAG AACAGCTTTC CTCCTTGTTC CCTCCAAACC ACACTCCTGC TTACTGACAT
  721 CACAACTCCC TGACACCCCT TAATCAACAC ACACACACAC ACACACACAC ACACACACAC
  781 ACACACACAC ACACACGAAA AAGAGCCCAC TCTATACAGC ACTCTGGCTA CAGCAAGTTC
  841 GGANCCCATG NCCCCCCAGC CCGAANNNGA TCCCTCCTGN GGNTCCNANA NGANACACTC
  901 CCCACAANCG NNANTTTGNT GGNTTGNNTG NGTGCTNGTT TTTGANNNTG NCCGTTTGAN
  961 GGTGNNGNTT NGNNCTGNTT CTGNNNNNNN ANNNNNNGNT TTTGNANTNN GNCNNTGNTN
1021 TNANNAANNN NNTTTNNNAT NCNNNNAAAG TTTCNNNNNN NNNN
//
Primer: hGHpolyA-R (Pr0350)
Region: hGH p(A), tTA 3'
Sequence file: RDB13211_A5Cib.seq check
>D02088F1_A5Ci_1_hGHpolyA-R_C10_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NAATANTATA GAAGGNNACC TAGTCAGACA AAATGATGCA
   61 ACTTAATTTT ATTAGGACAA GGCTGGTGGG CACTGGAGTG GCAACTTCCA GGGCCAGGAG
  121 AGGCACTGGG GAGGGGTCAC AGGGATGCCA CCCGTAGATC TCTCGAGCTA CCCACCGTAC
  181 TCGTCAATTC CAAGGGCATC GGTAAACATC TGCTCAAACT CGAAGTCGGC CATATCCAGA
  241 GCGCCGTAGG GGGCGGAGTC GTGGGGGGTA AATCCCGGAC CCGGGGAATC CCCGTCCCCC
  301 AACATGTCCA GATCGAAATC GTCTAGCGCG TCGGCATGCG CCATCGCCAC GTCCTCGCCG
  361 TCTAAGTGGA GCTCGTCCCC CAGGCTGACA TCGGTCGGGG GGGCCGTCGA CAGTCTGCGC
  421 GTGTGTCCCG CGGGGAGAAA GGACAGGCGC GGAGCCGCCA GCCCCGCCTC TTCGGGGGCG
  481 TCGTCGTCCG GGAGATCGAG CAGGCCCTCG ATGGTAGACC CGTAATTGTT TTTCGTACGC
  541 GCGCGGCTGT ACGCGGACCC ACTTTCACAT TTAAGTTGTT TTTCTAATCC GCATATGATC
  601 AATTCAAGGC CGAATAAGAA GGCTGGCTCT GCACCTTGGT GATCAAATAA TTCGATAGCT
  661 TGTCGTAATA ATGGCGGCAT ACTATCAGTA GTAGGTGTTT CCCTTTCTTC TTTAGCGACT
  721 TGATGCTCTT GATCTTCCNA TACGCAACCT AAAGTAAAAT GCCCCACAGC GCTGANTGCA
  781 TATAATGCAT TCTCTAGTGA AAAACCTTGT TGGCATAAAA AGGCTAATTG ATTTTCGAGA
  841 GTTTCATACT GTTTTTCTGT ANGCCGNGTA NCTANNTGTA CTTTTTGCTN CNTCGCGATG
  901 ANTTANTAAA GCANATCNAA AACTTTTANC GTTANTACGN NAAAAANTCN NGNCANCTTN
961 CCNNNNNTAA ANGGNNAAAA NNGANNATNG NNNNNANNNN NNNNNN
//
Primer: TetR-N_R (Pr0764)
Region: tTA 5', FLAG, Enk pro 3'
Sequence file: RDB13211_A5Cic.seq check
>D02088F1_A5Ci_1_TetR-N_R_B10_04_ABI24.ab1
    1 NNNNNNNNNN TTNNNNNNNG NNNNTACNAC GTAGGCTGCT CTACNCCTAG CTTCTGGGCG
   61 AGTTTACGGG TTGTTAAACC TTCGATTCCG ACCTCATTAA GCAGCTCTAA TGCGCTGTTA
  121 ATCACTTTAC TTTTATCTAA TCTAGATGGA TCCTTGTCAT CGTCGTCCTT GTAGTCGAGA
  181 TCCCCCGTCT CTTGTTCCAT GGTGGCGCTA GTAACGGCCG CCAGTGTGCT GGAATTCGCC
  241 CTTGCCTCAA TGAGAGCTTG GACCCTAGTG TTTCCTGCTG TTCTAGTGAT GCCTGGGACT
  301 ATTCTATCTT CCTGGAGGTA TCCTATCTTC CCACGGGGAG TCCTGTTTTC CTGATGACAT
  361 CCTGTCCTTC CGAGGGCGCC CTGTCTTCCC TGGCGTCGGT TGCTTAGGGC AAGATTCTGG
  421 AGCGAACACT GAAGTTCGCG GCTCGGGAGC AGGCGCTTGG GGGAATAGGA CCCGGACACC
  481 CAAAGCAATG AGGAACCCCG AGAAAGTTGG ACGAACGCAT GCACCGAGGG AACAGGCGAC
  541 GGTCGCCAAG GCACGGGCGA CTTGGGGCAA AGTCACTCAC GCTGCCGTTG CTCCAGATGG
  601 CGCAGGTTAC TGGAAGAGCG TGTACCAGAT GCCACTCTGC AAGAGAAAGA AAGAAGGCAA
  661 GTGTAAAGCG GCCGCGTCGG GGAGCTCTGC GAGGCGCCGG GCAGCGTCGC TGGGCAGCGG
  721 GGCTCTGTGC GCCTGCCTTG CTGGCGGCGG CCACAGCCAC TTTATAATTA ACCCCAAACC
  781 GAANGANGCG CGCGCGCCCC NATCGCCNGC GGGCTGCAGC TGANGCANGC CCTACGCCNA
  841 CCCGCGCCGA CGCCTANCGG GANGGGANGT CCCCCCCAGC ACGCGGCCCC NANGGNGGCN
  901 GGNGCGANNG GGCGCCNNNN GNNNCNCCNA CCNANCANTT ANGNNNTCNN GGGGNNGNNN
961 NGNANNGGNA AACTGNNTTC CNNNCNNNNN NNNNTNNNNN NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, Enk pro 5'
Sequence file: RDB13211_A5Cid.seq check
>D02088F1_A5Ci_1_CMV_Forward_A10_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN NNGCNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
  121 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCGCGGA TTCGAATCCC GGCCGGGAAC
  181 GGTGCATTGG AACGCGGATT CCCCGTGCCA AGAGTGACGT AAGTACCGCC TATAGAGTCT
  241 ATAGGCCCAC AAAAAATGCT TTCTTCTTTT AATATACTTT TTTGTTTATC TTATTTCTAA
  301 TACTTTCCCT AATCTCTTTC TTTCAGGGCA ATAATGATAC AATGTATCAT GCCTCTTTGC
  361 ACCATTCTAA AGAATAACAG TGATAATTTC TGGGTTAAGG CAATAGCAAT ATTTCTGCAT
  421 ATAAATATTT CTGCATATAA ATTGTAACTG ATGTAAGAGG TTTCATATTG CTAATAGCAG
  481 CTACAATCCA GCTACCATTC TGCTTTTATT TTATGGTTGG GATAAGGCTG GATTATTCTG
  541 AGTCCAAGCT AGGCCCTTTT GCTAATCATG TTCATACCTC TTATCTTCCT CCCACAGCTC
  601 CTGGGCAACG TGCTGGTCTG TGTGCTGGCC CATCACTTTG GCAAAGAATT GGGATTCGAA
  661 CATCGATTAG CGACCAAAAT GGGAGACGCA CTTCAGATTG CGTCTCTCAN GAGCTGCGTG
  721 AAGGCAGGAA CTGCAAAGAT TGAAAAGGGT GGANGATCCC TTGCAAATGT CTGGGAAAGC
  781 CTTGAGACCC TCTCTTTGTT GTAGCCTATT TGGACTANGA AGATAGGTAC ACACAATGGG
  841 TAAATCTTTG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAA
  901 GANNNCTGGN NNCAGATNNN AAGGAGANNN GGNTTAAAAC AGCTTTTGNN NNCNGANNGG
  961 NTNAAATGNT TTCGTTCATN NNTNNNNANG ANTNTGNNNN NAACNNNGNG NNNNNNNNNN
//
Primer: ColE1ori_F (Pr0393)
Region: ori, AAV2 ITR
Sequence file: RDB13211_A5Cie.seq check
>D02088F1_A5Ci_1_ColE1ori_F_D10_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCTGNNT TGANCGTCGA NTTTTGTGAN GCTCGTCAGG
   61 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
121 CTGGCCTTTT GCTCACATGT CCTGCAGGCA GCTGCGCGN
//
Primer: f1ori_F (Pr0280)
Region: f1 ori, AAV2 ITR
Sequence file: RDB13211_A5Cif.seq check
>D02088F1_A5Ci_1_f1ori_F_E10_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NGNAGCGGTC NNGCTGCGCG TAACCNCCAC ACCCGCCGCG
   61 CTTAATGCGC CGCTACAGGG CGCGTACTAT GGTTGCTTTG ACGTATGCGG TGTGAAATAC
121 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCC CTGCAGGCAG CTGCGCGNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hikida, T., Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior. Neuron 66 (6): 896-907 (2010). PMID 20620875. [link to RRC of NBRP]