Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmGR 5 (#RDB13199)

Plasmid clone of rat mGluR5 cDNA

Clone info. Plasmid clone of rat mGluR5 cDNA.
Vector backbone pBluescript SK(-) (Plasmid)
Selectable markers Amp^r
Gene/insert name Rat mGluR5 cDNA
Depositor|Developer Nakanishi, Shigetada |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, T., J. Biol. Chem. 267: 13361-13368, 1992) and an acknowledgment to the DEPOSITOR are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Abe, T., J. Biol. Chem. 267: 13361-13368, 1992) を引用し、謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13199 pmGR 5 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmGR 5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13199).

Reference section:

Abe, T., Sugihara, H., Nawa, H., Shigemoto, R., Mizuno, N., Nakanishi, S., Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction. J. Biol. Chem. 267: 13361-13368 (1992). PMID 1320017. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13199_A9Dgp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: insert 5'(Rat mGluR5 5')
Sequence file: RDB13199_A9Dgg.seq check
>13199_13199_A9Dg_2_M13_-40_A06_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNNN NNNNNNNNNC TATAGGGNNN NNNGGGTNCC GGGCCCCCCC
   61 TCGAGGTCGA CGGNANCNAT AAGCTTGATA TCGAATTCCG TTGCTGTCGA TACCGCCCTC
  121 AGCTCGGCTG TTCTGAGCAC TCCGAGCACA GGGGATGAGC TTGAAATCAT CTTGGGGTGG
  181 GGAAAAGCCC AGGACGGGAA AAGCTGGCTC AACCCCAATG ATTTCTGCTG CCCAACTTTA
  241 CTCCGGGGGG GCTAGCTAGA CAGCATCTCA CTGCCTGCTG CGTGGCCGAC AGGGGGTGAC
  301 TTAACTCAAC TTCTTGGTTT AGAAAAAAGG AACCTCTGTA TGCTGTTGCC TCCCACAGTT
  361 TTTCTACAAT TCAGACATCT CTTGAGAAAC TCAGCCTAGT TTATCCAGCT GTTTTGTCCA
  421 CATATGTTCA TAAAATCTAG TTATGAATAT TCTGATCGTA TGGATGCACC TAACTCGACA
  481 GTTTCTGGGG ACTTTGCTAT CTTTTAGAGA ATATATCTAA ATTGCTGACT AATTCCTCAA
  541 TGCGCCACTC AACGCAGGGC ATCATTTGGC TGTACCTATT GGAGCTCTGA GTTTCCTCCA
  601 TCATGATATC TTTATTAGCT TGAATTCCTT TCCTAAAATG GTCCTTCTGT TGATCCTGTC
  661 AGTCCTACTT CTGAAANANN ATGTACGAGG GAGTGCACNN NNNNGTGAGA GGAGGGTGGT
  721 GGNTCACATG CCANGTGACN TCNNNNNNGG AGCTCTCTTC TCTGTCNNCC ANCAACCAAC
  781 TGTGGNNNAA AGTTCATGAG AGGAAGTGTG GTGCAGTTNN TGANCAATAT GGGATNCAGA
  841 NANNNNAGGN CANGCTGCNT NNNNNNNNNA ANNGATCAAC TNNNANNCCA CNCTNNTGCC
  901 CAANNNNACN CTTNNNNTGN NNNNANNNNN AGNNTNCNGC TNGNNNTNCN NCTNNNNNNN
961 NNN
//
Primer: mGrm5_R4 (Pr0419)
Region: insert mid(Rat mGluR5 3')
Sequence file: RDB13199_A9Dgh.seq check
>13199_13199_A9Dg_2_mGrm5_R4_G02_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCTCCTGG NNNNNNNTCC NAATATAGAA GCAGTGTTTC
   61 TTAAAAGCCC ATGTTTTCTA AGGCCACTAG AAGATAATCT GCCAGAAGAT GTGTAGTTGG
  121 TGTCGTCATC GGCCGTCATG AAATAGCACT GTTGATCTCA TGTCTCCACC GTGTGTGTCC
  181 TTGGCATTTT TCACCTCGGG TTCTCTGTTG GGGTGACTAT GGGTGTCGCT GCGCCCATCT
  241 GTGAACACCG AGGTCTGCTG CTCAGGCCTA TCTCAGGGAA GTTTCCAGTG GCTCACAACG
  301 ATGAAGAACT CTGCGTGTAA TCTCTGATGA TGAGAGTGTC ATATTTGGGA GAGGATGGGA
  361 TGCAGAGGGC CGATTCTGAG ACTGGAGAGT TTGGGGTGGT GCTCCCCGAG TCCACCGAGT
  421 CCCTGAAGGG CGATGGTGGA GTGAGGGCCA CAAGCTCCTC CAGATCTGGT TTGCCCGGGG
  481 CGGATTCAGC TCCTGTGGGC GTCTCCTGGG CAGGTGATAC GCCTGTCGCC CCCTGAGCTC
  541 CTCCGGTCAC CTCGATGGCC GGCAGCGGCT GGATCTCTGC GAAGGTCGTC ATGGTCGTGG
  601 GCAGCTGAAT CTCTTTGGGG ATCAGGTAGG AGGAGCAGAT TGGAGTACCA GGGGGCCCCG
  661 GCGTGGCCGC GGTGGACAAC ATCATGGAGT TGAGCTCGGA GATGTTGGCT GTGAANNNNN
  721 NCNNNNNNCT GNNNNNNTGC TNCNNNNNNN GAGCCCTGGG ACGAGCTGCT GCNAGCAGCT
  781 GTCNNNNNNT GNANCGACNG CGCATCATCA TCTGTGCGGC CCGCCGAGCC TGCGANGTGG
  841 NTNNNNNTGC TGATGGGCGA NNGGNNANNG CNGNCTNNNN NCCNNGNNGA ANNCTCTCCT
  901 NTNNNNTCCN NNNNNNCANN AAAGCGCTTT NNNNNNNNNG NNNTCCNGNN GNNNNNNGGC
961 CACCTGNNNG NNGNNNNNTN NNNGNNTNNC NNNNNNNNNN
//
Primer: reverse-2 (Pr0100)
Region: insert 3'
Sequence file: RDB13199_A9Dgi.seq check
>13199_13199_A9Dg_2_Reverse2_B06_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNAACCCTCA CTANAGGGNN NNNNNNNNTG
   61 GAGCTCCACC GCGGTGGCGG CCGCTTTTTT TTTTTTTTTT TCTTAAAAAG TGTGTTTAAT
  121 TCCTGGTTTG GAAGTCACAG ATTACAGCAG AGCCATTACA CAATGCATTT CAGTCATAAA
  181 CAGCTCCGAG TCTCTCTGAA CACTTGCTGG TGTATCAGTC ATTTGGAAAA TACTATCACG
  241 TTCAAAGATT GGCACGTTTG GAGACTTGCT TTGTCTTTCT CCTTTGTGTC TGTTTATTTC
  301 TTATGAGTTT CCTCTCCNGA TTCCCTTCCT ATTCCCTATG TCTAGGTCCA GACAAACATA
  361 GCCTCTCAGC CTGACTCTAC TTTCCAACAG GAAGGGGAAT GGACCTTTTA GTTGATAAGC
  421 TTAATTAAAG CGGGTCTTTC CTTCCCAGGA CCAAGTTCTC CTTTACTGGT CATTAACATT
  481 TTCATTCTCC CTTTAAGTTG TGCATATTCN NNNNGTTTAT CACTGGGTAC CAGGAACAGA
  541 TGGGTAGCAA GGAAACTCAG TAAGAACAGG TACCCAAATA CATTGGGAAN NNNGTGTCCT
  601 CGCCCTAAAA TGAATATTCA GTTCTCNNNN NNNGACAGTT ATTACTTGGA ACCCGGTGTT
  661 ANAACTGGCA AAGAGAGNNN NNNNNTCATT CCAAANNNNN AGANTTCAAA GATNNATTAC
  721 CGGCATGTGC AATATACAAT GTNTTCAATG AGATGGNAAA CGTTATNNNN NNATACAACN
  781 AANNNNNNAT TNNNNTNTCC NNNNNNNNNA NNAAACTTTT ATTACAGTTG ACTNTGATAT
841 CACNNNGGAA NNTNNNNATG GGNAATNNNN NNNNTCTCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Abe, T., Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction. J. Biol. Chem. 267: 13361-13368 (1992). PMID 1320017. [link to RRC of NBRP]