Resource data sheet
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pmGR 4

Plasmid clone of rat mGluR4 cDNA.

Catalog number RDB13198
Resource name pmGR 4
Clone info. Plasmid clone of rat mGluR4 cDNA. Refer Fig.1c of Tanabe, Y., Neuron 8 (1), 169-179 (1992).
Vector backbone pBluescript SK(-) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Rat mGluR4 cDNA
Reference of nucleotide sequence M92077.1 (DDBJ accession number)
Depositor Nakanishi, Shigetada |
 
Other clones in our bank Rat Grm4 (KEGG orthology K04607) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Neuron 8: 169-179, 1992) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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RDB13198 pmGR 4 DNA solution Please contact us.

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References and tips

Electronic file

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References

original Tanabe, Y., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649.

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13198_A5Cip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB13198_A5Cia.seq
>13198_13198_A5Ci_Reverse2_D11_11_ABI24.ab1
    1 AGGCCATCAT TACGCCAGCT CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGAGCTC
   61 CACCGCGGTG GCGGCCGCTC TAGAACTAGT GGATCCCCCG GGCTGCAGGA ATTCGGCACG
  121 AGCTGAGCTC CCCCGGCGCA GCCGACCCCT GGTCGCGGCG TTCACCTCGC CGATGCGCGG
  181 TTGGTAGGAG TGACCGGAGC CATTCTCTCC TCGTTGATAA GATTCCCTAC CAGGATAGGA
  241 GCCTATCTCC CTTTCCACAG CAGGACACAG AAATCTGGCC TTCAGTACTT TGGGAAAAGG
  301 ATCTGAGACC TCCCGGAGCT CTGACCACTG GCTGTCATCT GTGGCTCTGG CCTGTGTGGG
  361 CCACTGAGCT CTACTCAAAC ATTAAAGAGG AGGAGGGGAG ATCTGTGGAA TGGGCCACCC
  421 CGTTGGCCTG CTGCATTACT GAACCTGCGC TGTCCACACG CGCCCAGATC ATGGGACCCA
  481 GGGCCTGCTA GGGCTAGGAG CGGGGCCCAG TATTCATGGG TCTCTAGGCC TTTCCGAAAT
  541 GTCCGGGAAG GGAGGCTGGG CCTGGTGGTG GGCCCGGCTG CCCCTCTGCC TACTCCTCAG
  601 CCTTTATGCC CCCTGGGTGC CTTCATCCTT GGGAAAGCCC AAGGGTCACC CCCACATGAA
  661 CTCTATCCGA ATTGACGGGG ACATCACACT GGGAGGCCTG TTTCCCGTCC ACGGCCGTGG
  721 CTCTGAGGGT AAGGCCTGCG GGGAGCTGAA GAAGGAGAAA GGCATCCACC GCCTGGAGGC
  781 CATGCTGTTT GCCCTGGACC GCATCAACAA TGACCCGGAC CTACTGCCCA ACATCACGTT
  841 GGGCGCCCGC ATTCTGGACA CCTGCTCGAG GGACACCCAC GCCCTGGAGC AGTCACTGAC
  901 CTTTGTGCAG GCGCTCATCG AGAAAGGACG GCACGGAGGT CCGCTGCGGC AGCGGGGGGC
  961 CCGCCCATCA TCACCAAGCC CGAACGAGTG GTGGGTGTCA TTGGAGCTTC AGGAGCTCCG
 1021 TCTCGATCAT GGTGGCCCAA CATCCTCCGC TCTCAGATCC CTCAGATCAG CTATGGCTCA
 1081 CGCCCCTGAC TGAGTGACAC AGCGCTATGA CCTTCCTCTC CTGGGTGGTG CCTTCAGAAC
1141 ACCATTACCA GGG
//
Primer: T7_20bp RDB13198_A5Cib.seq
>13198_13198_A5Ci_T7_20bp_E11_14_ABI24.ab1
    1 TGCAGGGACC CCTCGAGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCG GCACGAGTTT
   61 TTTTTTTTTT TTTTTTTGCA AAACCATGTG TTTATCTTTA TTAGAAATGT TCTCGGTATA
  121 AAAACAGACA TGCTACACAT CAGAGACCAT CACCAAACAC CCAGGCACTG AACTCCGTGT
  181 CGTTAGCAGG AAGGGCAAGG CGGGCAGGCG GGCGGGCAGG ACACACGGCA GACGGGTAGG
  241 GCGGGCGAGC AGGCTGCAGG CCACAGGGAC ACACAGGCCA CCAGGAGGAC GCAGCACTTC
  301 GAAACACACT CAAGATTAGT TTTTGTTTGG GCTTTTTTGT AGGGGGTGAT TCCCCCCCCC
  361 CTTTTTTTTT CTCTTTTGCA ACGTGGTGAA AGAGTTGGTG GATGAAGCCA AGTAGGGGGA
  421 AGGCTGGTAG GAAATGAGAT CGAGGGGGAA CAGAAGAGAA AATGATGGCG GGGATGGGGC
  481 AGCTCAGGGA GGAGGGGCAG GGGGCAGGGC AGATGGCAGG GGAAGGCCAG GAGCAGAATG
  541 GAAAAATATG GAAGCAGAGG GCCCAAACAG ACAACACATG CAGAGCAAAG ACGGAAGAGA
  601 AACGTAAAGA CGGGGCAGGG ACCAACCGGG GTCACCCACA GGACAGACGG AGGCAGGAGG
  661 AAGGAGAGGA AGCCAGGGCT GGGCTCTGGA GACCGAGCCC CTGTGCTTTG GGGCTGGGCC
  721 CAACAGTGGT CCAGAAGGAA GACCTGTGTG TGGCATAACC TGGAACTGCC CCAGCCACTC
  781 GGCCTCCTCT GGTTGCCCAC CACGGCTTGC CCAGCAGTGA TGGCCGGGGT CTCTACTGTC
  841 CTCAGCACCA AGCCACATTC GTGGATGCCC GGGAGGCGGG CAGACATCGG CAGGGCCCTC
  901 GGGAACGGCC CTGGCCCAAT GTGCCATCCT CAGAGGTTGT GGCTCCTTTA GCCTGCTTGG
  961 CTCCGCGGCC CGGGCTAGAT GGCATGGTTG GTGTAGTGAC GTAGTCTGTT TGGTAGCAGC
 1021 GCTGGGGTCT CCAGGGTCTC ACACAGCTCT GATTGGCTTC CCATGGGGCT GGAGTGCCCT
1081 CCTGTGTGAC CTGGTGAAAT GTGCAGCGTG ACACGCTTGA GACTGCGCTT GCGCCTTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tanabe, Y., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649.

2018.12.10

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