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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFPN1/Gmip (#RDB13127)

Expression plasmid of Gmip-full length cDNA, GFP tag.

Clone info. Expression plasmid of Gmip-full length cDNA, GFP tag.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Gene/insert name Mouse Gmip cDNA
Depositor|Developer Sawamoto, Kazunobu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nat. Commun. 5: 4532 (2014)).
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nat. Commun. 5: 4532 (2014))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13127 pEGFPN1/Gmip DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFPN1/Gmip was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13127).

Reference section:

Ota, H., Hikita, T., Sawada, M., Nishioka, T., Matsumoto, M., Komura, M., Ohno, A., Kamiya, Y., Miyamoto, T., Asai, N., Enomoto, A., Takahashi, M., Kaibuchi, K., Sobue, K., Sawamoto, K., Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA. Nat. Commun. 5: 4532 (2014). PMID 25074242. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15925_A8E8p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15925_A8E8a.seq check
>13127_15925_A8E8_1_CMV-Forward_A02_02_ABI24.ab1
    1 GAGCTGCCAA CTTCCCGCCC CATTTTGAAC GCAAATGGGG CGGTAGGCGT GTACGGTGGG
   61 AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC
  121 AGATCTCGAG CTCAAGCTTC GAATTCTGCA GTCGACATGG ACTCAGCGGA GACAGAGCTA
  181 ACCCCAGCTC CTGAGGGCAG GAAGAGATAC AGTGACATCT TCCAGAGCCT GGACAACCTC
  241 GAGATATCAT TGGGGAACGT AACTTTTGAT CCCCTGGCTG GAGACCCTGT ACGCAGAGAA
  301 GACCTGGAGC CTGACAAAGC TGACACAGCC ACAGTGGTGA CTGAGGAGAA CAGTGAAGCC
  361 AGTAGTTGGC GGGATCTCTC CCCAGAAGGT CCTGCACCCC TCACAGAGGA AGAACTGGAT
  421 TTGCGACTCA TTCGGACCAA GGGTGGTGTA GATGCTGCCC TGGAGTATGC TAAAGCATGG
  481 AGCCGCTATG CCAAGGAACT GCTGGCCTGG ACAGACAAGA GAGCCAACTA CGAGCTCGAG
  541 TTTGCTAAGA GTATCATGAA GCTCGCTGAG GCTGGCAAGG TGTCCATTCT CCAGCAGAGC
  601 CAAATGCCAC TCCAGTACAT CTACACCTTG TTTCTGGAGC ATGACCTCAG CCTGGGAGCC
  661 CTGGCCCTGG AGACAGTGGC CCAACAGAAG AGAGACTACT ACCAGCCTCT GGCGGCCAAA
  721 AGGATGGAAA TTGAGAAGTG GAGGAAGGAA TTCAAGGAAC AGTGGCTGAA GGAGCAGAAG
  781 CGCATGAATG AGGCAGTGCA GGCACTGCGG CGCTCCGAGC TCCAGTACAT TCAACGCAGA
  841 GAGGACCTGA GGGCACGCTC CCAGGGGGTC CCCTGAGGAC CCTCCTTCCC AGGCATCTCC
  901 TGGATCCAAC AAGCAACAGG AGCGCAGACG CGCTCCCGAG AGGAGGCACA GGCCAAAGCG
  961 CATGAGGCGG GAGGCTCTGT ACCAGGCCTG TGTCCCGGGA GGCCAATTCT CGCCCAGCAG
 1021 GATCTGGAGA CCACCAAGCG GCGGGATAGT GTCACATGTG CGCAAGCTGG TTGCTGCAAG
 1081 AGACGAGTGC TTTAAGCGGT GAACGCTTGT CTTGTTGAGC CTGCGAGGGG CGCAAGGCCA
1141 AGAGAAGCCG AGAG
//
Primer: YFP_R
Sequence file: RDB15925_A8E8b.seq check
>13127_15925_A8E8_1_YFP_R_B02_05_ABI24.ab1
    1 TGTCCATCAT CGCCCTCGCC GGAACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT
   61 CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGCGA
  121 CCGGTGGATC CCGGGCCCGC GGTACCGTCG ACGGTATCGA TAAGCTTGAT GCGGCCGCGA
  181 GGTGCTCTTC AGACTCCTCA GGCTCTGGGT CAGCACAACA GGTGGTCCTG GACACAGCAT
  241 CACTGTTCAG CTTGGAGAGA AGTCGGGCTG TCTCCTGGGT GATCTCAAAG TGCTTGGGCA
  301 GAGGGTTCCG GCGCAGGGGG CTGCCTTCAG GGCAGGCAGC AGCAGGGCCC AAACCTCGTA
  361 CCCTCAAACT ACCTCGGTGC ACTGCTGAGA CAGTGACAGG AGTCTCCTCA CACAGCTTGG
  421 AGGCCACCAG AGCCAGGCTG GAGAGTTGAT GAGTAACTGG CCTCACACCT CCCCGGGAAT
  481 ACTTCACTGG CTGGCGGCTG AAGTGGCCCC GGGATTGTGC TCCCAGGGCC AAGTCCTCAG
  541 GGCAGTGGCT GGACCCATCC CCTGCCCCAT CTCTGGTGTC TTCCACCTCC TCCTCCCTCT
  601 GGTCCCTCTG CAGAGTTGCA AGCTCAGGAG GTGTGACCTC GGGGCACTTC TCCAAGGCAC
  661 TGTGGTGTTT GGGGTCTGGG CTGGAATCCA AGGCTATGGT CAGGGGCTGT GTGTCTTGGG
  721 CAAGAAGTGA GGCTGGGTGC TGGGGACTGG ATTCAAGGCA TGCAGGAGCC AAGCCAGGGT
  781 CTTGGGTCAG AGGCTCCGAG GCCAGAGGGA GCTCATCCAT TCCAAAGATC TGCTCATAGT
  841 GCACAATGAG GAACTCAACC AGCTGAGCCT GGTGACCGGA GTCCAGCAGA CAGGCCACAG
  901 GGCTGGCACC GGGTGGCCCT GGGTCCATCT GGTGGCCGCA GCAGTGTAGG CCCAAATACA
  961 ATTCCCAGAT TGTTGGCAGA CATCTTTGTT TTCTTCAAAC CGAGCAGCCC ACCCTGAACA
 1021 GATGGGCCAC CAGGTGTCGC AGGTACTGTA GTTAGAGGTC AGGCAGCTGC ACAAGAGGGA
1081 TCTTCAGCGA GCCGATATCT CAGGCTGGGG ATTGGGGGGA TTCTCAAGGG GATCG
//
Primer: Gmip-M_R
Sequence file: RDB15925_A8E8c.seq check
>13127_15925_A8E8_1_Gmip-M_R_C02_08_ABI24.ab1
    1 ATGGGTCTTT GGGGGAAGAA GTGAGGCTGG GTGCTGGGGA CTGGATTCAA GGCATGCAGG
   61 AGCCAAGCCA GGGTCTTGGG TCAGAGGCTC CGAGGCCAGA GGGAGCTCAT CCATTCCAAA
  121 GATCTGCTCA TAGTGCACAA TGAGGAACTC AACCAGCTGA GCCTGGTGAC CGGAGTCCAG
  181 CAGACAGGCC ACAGGGCTGG CACCGGTGGC CCTGGGTCCA TCTGGTGGCC GCAGCAGTGT
  241 AGGCCCAAAT ACAATTCCCA GATTGTTGGC AGACATCTTG TTTTCTTCAA ACCGAGCAGC
  301 CACCCTGAAC AGATGGGCCA CCAGGTGTCG CAGGGTACTG TAGTTAGAGT CAGGCAGCTG
  361 CACCAAGAGG GTCTTCAGCG AGCGAATAAT CTCAGGGCTG GGGTTGGGGG TCCCAGGGTC
  421 GTCCCCAGGG TCTGCATGCA GGGTCTTTGC CAGAGAGATG AAGGCGTCGT ACAAGTGGAA
  481 GGGGACCACG GGATCAGTGA GCTCCTGAAG AAATCGCTTG AGAACGCTGG TGATATCGTG
  541 AGGAGAGTTC CCGGACAGCT CGACCAGTGC TCGGCCATTC TCAAAGGCCT GGCACAGCCG
  601 CTCCACACGT ACCCGAGACC CGCTGACCCG ATAGATACCC TGCAAGCCCA GGGCACGGTG
  661 CTCTATCTCA GCTGTGCATC TGGTAATCAC AAAGGGAACC TCCTCAGGGA AATCTCTGGG
  721 GAGCTGTAGG AAGTCAACCC CAAAGAGGGA CATCCGGGCC GGAAGCCGCC GGTGTCCACA
  781 AAGGATGAGG AGGGTCTCCA GGCAGCGCTT GTGACAGGTC AAAAAGCACT CTTCACATTC
  841 TGTCCCGCTG ACCATGAAGG CTTCACATTC TCTGCACTTG GCTGGGCCCC GCAGCCGCCG
  901 CAGTCGGTGG GTTTGAGCAG CTGTGGACAG TGTCCACTTC CTGAACGGGC TGCCTACTCC
  961 ATTTCTCTAT CCCATCCCCC CAGATCAGGG TCTCGGCTCC TCAAAGTCAT CGGAGGACTC
 1021 CGTGCCCTGT GGACGAAGCC CTTTTACAAG TCGGCGGGCC CCCAGCACCT GGGCTGGGAA
 1081 AGTGGGGAGA ATCCCAAAGG ACCGGGAACT CACTGCAACA CTTAACACTG TCCACATCAC
1141 CTGGCCTGTG GGAATTCCCG TGG
//
Primer: SV40polyA-R
Sequence file: RDB15925_A8E8d.seq check
>13127_15925_A8E8_1_SV40polyA_R_D02_11_ABI24.ab1
    1 AAAAGGGGGA AGGTTTTTTA GCAGTAAACC TCTACAAATG TGGTATGGCT GATTATGATC
   61 TAGAGTCGCG GCCGCTTTAC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG
  121 TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG
  181 ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT
  241 TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA
  301 AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG TGGCTGTTGT
  361 AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA
  421 GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT
  481 TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT
  541 TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT GAAGCACTGC ACGCCGTAGG
  601 TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT
  661 TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT
  721 GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT
  781 CGCCCTTGCT CACCATGGTG GCGACCGGTG GATCCCGGGC CCGCGGTACC GTCGACGGTA
  841 TCGATAAGCT TGATGCGGCC GCGAGGTGCT CTTCAGACTC CTCAGGCTCT GGGTCAGCAC
  901 AACAGGTGGT CCTGGACACA GCATCACTGT TCAGCTTGGA GAGAAGTCGG GCTGTCTCCT
  961 GGGTGATCTC AAAGTGCTTG GGCAGAGGGT TCCCGCGCAG GGGCTGCCTT TCAGGGCAGC
 1021 AGCAGCAGGG CCCAAACCTC GTACCCTCAA CTACCTCGGT GCACTGCTGA GACAGTGACA
 1081 GAGTCTCTCA CACAGCCTGG AAGCACAGAG CAGCCTGAAG TGATGAGTAA CTGGGTCTTC
1141 A
//
Primer: SV40pro_F_V2
Sequence file: RDB15925_A8E8e.seq check
>13127_15925_A8E8_1_SV40pro_F_V2_E02_14_ABI24.ab1
    1 GAGCAACGTT TTTTTGGGAG GCCTAGGCTT TTGCAAGATC GATCAAGAGA CAGGATGAGG
   61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
  601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG
  901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AGGTTGGGCT
  961 TCGGAATCGT TTTCCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
 1021 GAGTTCTTCG CCCACCCTAG GGGGAGGCTA ACTGAAACAC GGGAAGAGAC ATACCGGAGA
1081 CCCGCCGCTA TGACGGCCAT AAAAGGACAG ATAAAAACGC CACCGGGATG TGTTTGCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ota, H., Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA. Nat. Commun. 5: 4532 (2014). PMID 25074242. [link to RRC of NBRP]