Resource data sheet
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CS-CDF-PGKG-PRE

Lentivirus vector construct of GFP driven by mouse PGK promoter.

Catalog number RDB12968
Resource name CS-CDF-PGKG-PRE
Clone info. Lentivirus vector construct of GFP driven by mouse PGK promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12968 CS-CDF-PGKG-PRE DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12968z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12968_A9I4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: EGFP,PGKpro | Primer: EGFP-M_R | Sequence: RDB12968_AI4a.seq
>12968_12968_A9I4_3_EGFP-M_R_H08_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGGNNCT TGNNGTTNNC CTTGNTGCCG TTCTTCTGCT
   61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
  121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
  181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
  241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
  301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG
  361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT
  421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
  481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GTGGCGACCG
  541 GTAGCGCTAG GGTCGAAAGG CCCGGAGATG AGGAAGAGGA GAACAGCGCG GCAGACGTGC
  601 GCTTTTGAAG CGTGCAGAAT GCCGGGCCTC CGGAGGACCT TCGGGCGCCC GCCCCGCCCC
  661 TGAGCCCGCC CCTGAGCCCG CCCCCGGACC CACCCCTTCC CAGCCTCTGA NCCCAGAAAG
  721 CGAANGAGCA AAGCTGCTAT TGGNCGCTGC CCCAAAGGCC TACCCGCTNN CNTTGCTCNN
  781 CGGTGCTGTC CATCTGCACN ANNNTAGTGA GACGTGCTAC NTTNCNNTTG TCACGTCCNG
  841 CNCGACNCNN NCTGCNGGGN NGGGGGGNNA ACTTTNNNGA CTANGGNANG NNTANNANGN
  901 GGNNCNANNG GGNCNNCCNA ANNAACNNNA NNCNGNNGGC GNCNNNCNNN TNGGANGNNG
  961 GAAANNGNGN NNNNANGNCC NNAANGNCCA NNTNGTGNNN NCGNCNAANN NNCCNNNNGG
1021 GGGNNNNNNN ANNNNNN
//
Region: WPRE,EGFP | Primer: PRE_rev | Sequence: RDB12968_AI4b.seq
>12968_12968_A9I4_3_PRE_rev_F12_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNNGAGCAAC ATAGTTAAGA NTACCAGTCA ATCTTTCNCA
   61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGATC CTACTAGTAG
  121 GATCTGAGTC CGGACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
  181 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
  241 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
  301 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC
  361 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG
  421 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
  481 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
  541 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
  601 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTANGTCAGG
  661 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG
  721 GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGNNCG
  781 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
841 TTGCTCACCA TGGTGGCGAC CGGTANCGCT N
//
Region: CMVpro,5'LTR,HIV-1psi | Primer: CMV enh_F1 | Sequence: RDB12968_AI4c.seq
>12968_12968_A9I4_3_CMVenh_F1_G12_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTG CCNNGNANNT GACCTTATGG GANTTTCCTA CTTGGCAGTA
   61 CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG
  121 GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG
  181 GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC
  241 ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GCGCGTTTTG
  301 CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG
  361 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC
  421 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA
  481 TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC
  541 TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT
  601 GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT
  661 CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA NNNCAGGGGG
  721 AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC
  781 AGTTAATCCT GGCCTGTTAG AAACATCAGA ANGCTGTAGA CAAATACTGN NACAGCTACN
841 ACCATCCCTT CAGACAGGAT CAGAAGAACT TANATC
//
Region: RRE,HIV-1psi,5`LTR | Primer: RRE-M_R | Sequence: RDB12968_AI4d.seq
>12968_12968_A9I4_3_RRE-M_R_H12_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG CTGTTGCGCC TCNATAGCCC TCAGCAAATT GTTCTGCTGC
   61 TGCACTATAC CAGACAATAA TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC
  121 ATAGTGCTTC CTGCTGCTCC CAAGAACCCA AGGAACAAAG CTCCTATTCC CACTGCTCTT
  181 TTTTCTCTCT GCACCACTCT TCTCTTTGCC TTGGTGGGTG CTACTCCTAA TGGTTCAATT
  241 TTTACTACTT TATATTTATA TAATTCACTT CTCCAATTGT CCCTCATATC TCCTCCTCCA
  301 GGTCTGAAGA TCAGCGGCCG CTTGCTGTGC GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC
  361 TATCTTGTCT AAAGCTTCCT TGGTGTCTTT TATCTCTATC CTTTGATGCA CACAATAGAG
  421 GGTTGCTACT GTATTATATA ATGATCTAAG TTCTTCTGAT CCTGTCTGAA GGGATGGTTG
  481 TAGCTGTCCC AGTATTTGTC TACAGCCTTC TGATGTTTCT AACAGGCCAG GATTAACTGC
  541 GAATCGTTCT AGCTCCCTGC TTGCCCATAC TATATGTTTT AATTTATATT TTTTCTTTCC
  601 CCCTGGCCTT AACCGAATTT TTTCCCATCG CGATCTAATT CTCCCCCGCT TAATACTGAC
  661 GCTCTCGCAC CCATCTCTCT CCTTCTAGCC TCCGCTAGTC AAAATTTTTG GCGTACTCAC
  721 CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA
  781 GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA GTCCCTGTTC GGGCGCCACT GCTAGAGANT
841 TT
//
Region: EGFP,PGKpro,cPPT_CTS | Primer: YFP_R | Sequence: RDB12968_AI4e.seq
>12968_12968_A9I4_YFP_R_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCTNANTTGT GGCCGTTTNC GTCGCCGTCC NGCTCGACCA
   61 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGGTG GCGACCGGTA
  121 GCGCTAGGGT CGAAAGGCCC GGAGATGAGG AAGAGGAGAA CAGCGCGGCA GACGTGCGCT
  181 TTTGAAGCGT GCAGAATGCC GGGCCTCCGG AGGACCTTCG GGCGCCCGCC CCGCCCCTGA
  241 GCCCGCCCCT GAGCCCGCCC CCGGACCCAC CCCTTCCCAG CCTCTGAGCC CAGAAAGCGA
  301 AGGAGCAAAG CTGCTATTGG CCGCTGCCCC AAAGGCCTAC CCGCTTCCAT TGCTCAGCGG
  361 TGCTGTCCAT CTGCACGAGA CTAGTGAGAC GTGCTACTTC CATTTGTCAC GTCCTGCACG
  421 ACGCGAGCTG CGGGGCGGGG GGGAACTTCC TGACTAGGGG AGGAGTAGAA GGTGGCGCGA
  481 AGGGGCCACC AAAGAACGGA GCCGGTTGGC GCCTACCGGT GGATGTGGAA TGTGTGCGAG
  541 GCCAGAGGCC ACTTGTGTAG CGCCAAGTGC CCAGCGGGGC TGCTAAAGCG CATGCTCCAG
  601 ACTGCCTTGG GAAAAGCGCC TCCCCTACCC GGTAGAATTC AAAGTGAATT TCTGCTGTCC
  661 CTGTAATAAA CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT
  721 ATGTCTGTTG CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCNNNCT
  781 TTTCTTTTAA AATTGTGGAT GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA
841 TCTGTCTCTG TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCNGGCCNN
//
Region: bGHp(A),3'LTR | Primer: BGH_rev2 | Sequence: RDB12968_AI4f.seq
>12968_12968_A9I4_3_BGH_rev2_E12_15_ABI24.ab1
    1 NNNNNNNNNN GNNNNNNGGG GGNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG
  121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
  181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
  241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC
  301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA
  361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT
  421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT
  481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG
  541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC
  601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT
  661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA
  721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA
781 CGGTATCGAT GCGGGGNGGC GGNC
//
Region: SV40p(A),Zeo,SV40pro_ori | Primer: pAxEF R2 | Sequence: RDB12968_AI4g.seq
>12968_12968_A9I4_pAxEF R2_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNTTT GTGANNTTNT GNNGCTATTG CTTTATTTGT AACCATTATA
   61 AGCTGCAATA AACAAGTTGG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG
  121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC
  181 GGTGGAATCG AAATCTCGTA GCACGTGTCA GTCCTGCTCC TCGGCCACGA AGTGCACGCA
  241 GTTGCCGGCC GGGTCGCGCA GGGCGAACTC CCGCCCCCAC GGCTGCTCGC CGATCTCGGT
  301 CATGGCCGGC CCGGAGGCGT CCCGGAAGTT CGTGGACACG ACCTCCGACC ACTCGGCGTA
  361 CAGCTCGTCC AGGCCGCGCA CCCACACCCA GGCCAGGGTG TTGTCCGGCA CCACCTGGTC
  421 CTGGACCGCG CTGATGAACA GGGTCACGTC GTCCCGGACC ACACCGGCGA AGTCGTCCTC
  481 CACGAAGTCC CGGGAGAACC CGAGCCGGTC GGTCCAGAAC TCGACCGCTC CGGCGACGTC
  541 GCGCGCGGTG AGCACCGGAA CGGCACTGGT CAACTTGGCC ATGGTTTAGT TCCTCACCTT
  601 GTCGTATTAT ACTATGCCGA TATACTATGC CGATGATTAA TTGTCAACAC GTGCTGATCA
  661 GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA
721 AAGCCTCCTC ACTACTTCTG GAATAGCTCA GANGCAGAN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.10.02

GNP_filter3_RDBDEP_html_190927.pl