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CS-CDF-PGKG-PRE

Lentivirus vector plasmid of GFP driven by mouse PGK promoter.

Catalog number RDB12968
Resource name CS-CDF-PGKG-PRE
Clone info. Lentivirus vector plasmid of GFP driven by mouse PGK promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB12968zzk02.seq provided by the depositor
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB12968 CS-CDF-PGKG-PRE DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CS-CDF-PGKG-PRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12968).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            References and tips

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12968_A9I4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EGFP-M_R (Pr0743)
            Region: EGFP,PGKpro
            Sequence file: RDB12968_A9I4a.seq check
            >12968_12968_A9I4_3_EGFP-M_R_H08_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNGGNNCT TGNNGTTNNC CTTGNTGCCG TTCTTCTGCT
               61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
              121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
              181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
              241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
              301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG
              361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT
              421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
              481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GTGGCGACCG
              541 GTAGCGCTAG GGTCGAAAGG CCCGGAGATG AGGAAGAGGA GAACAGCGCG GCAGACGTGC
              601 GCTTTTGAAG CGTGCAGAAT GCCGGGCCTC CGGAGGACCT TCGGGCGCCC GCCCCGCCCC
              661 TGAGCCCGCC CCTGAGCCCG CCCCCGGACC CACCCCTTCC CAGCCTCTGA NCCCAGAAAG
              721 CGAANGAGCA AAGCTGCTAT TGGNCGCTGC CCCAAAGGCC TACCCGCTNN CNTTGCTCNN
              781 CGGTGCTGTC CATCTGCACN ANNNTAGTGA GACGTGCTAC NTTNCNNTTG TCACGTCCNG
              841 CNCGACNCNN NCTGCNGGGN NGGGGGGNNA ACTTTNNNGA CTANGGNANG NNTANNANGN
              901 GGNNCNANNG GGNCNNCCNA ANNAACNNNA NNCNGNNGGC GNCNNNCNNN TNGGANGNNG
              961 GAAANNGNGN NNNNANGNCC NNAANGNCCA NNTNGTGNNN NCGNCNAANN NNCCNNNNGG
            1021 GGGNNNNNNN ANNNNNN
            //
            Primer: PRE_rev (Pr0250)
            Region: WPRE,EGFP
            Sequence file: RDB12968_A9I4b.seq check
            >12968_12968_A9I4_3_PRE_rev_F12_18_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNNN NNNGAGCAAC ATAGTTAAGA NTACCAGTCA ATCTTTCNCA
               61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGATC CTACTAGTAG
              121 GATCTGAGTC CGGACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
              181 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
              241 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
              301 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC
              361 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG
              421 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
              481 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
              541 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
              601 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTANGTCAGG
              661 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG
              721 GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGNNCG
              781 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
            841 TTGCTCACCA TGGTGGCGAC CGGTANCGCT N
            //
            Primer: CMV enh_F1 (Pr0652)
            Region: CMVpro,5'LTR,HIV-1psi
            Sequence file: RDB12968_A9I4c.seq check
            >12968_12968_A9I4_3_CMVenh_F1_G12_21_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNTG CCNNGNANNT GACCTTATGG GANTTTCCTA CTTGGCAGTA
               61 CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG
              121 GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG
              181 GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC
              241 ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GCGCGTTTTG
              301 CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG
              361 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC
              421 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA
              481 TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC
              541 TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT
              601 GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT
              661 CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA NNNCAGGGGG
              721 AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC
              781 AGTTAATCCT GGCCTGTTAG AAACATCAGA ANGCTGTAGA CAAATACTGN NACAGCTACN
            841 ACCATCCCTT CAGACAGGAT CAGAAGAACT TANATC
            //
            Primer: RRE-M_R (Pr0651)
            Region: RRE,HIV-1psi,5`LTR
            Sequence file: RDB12968_A9I4d.seq check
            >12968_12968_A9I4_3_RRE-M_R_H12_24_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNG CTGTTGCGCC TCNATAGCCC TCAGCAAATT GTTCTGCTGC
               61 TGCACTATAC CAGACAATAA TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC
              121 ATAGTGCTTC CTGCTGCTCC CAAGAACCCA AGGAACAAAG CTCCTATTCC CACTGCTCTT
              181 TTTTCTCTCT GCACCACTCT TCTCTTTGCC TTGGTGGGTG CTACTCCTAA TGGTTCAATT
              241 TTTACTACTT TATATTTATA TAATTCACTT CTCCAATTGT CCCTCATATC TCCTCCTCCA
              301 GGTCTGAAGA TCAGCGGCCG CTTGCTGTGC GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC
              361 TATCTTGTCT AAAGCTTCCT TGGTGTCTTT TATCTCTATC CTTTGATGCA CACAATAGAG
              421 GGTTGCTACT GTATTATATA ATGATCTAAG TTCTTCTGAT CCTGTCTGAA GGGATGGTTG
              481 TAGCTGTCCC AGTATTTGTC TACAGCCTTC TGATGTTTCT AACAGGCCAG GATTAACTGC
              541 GAATCGTTCT AGCTCCCTGC TTGCCCATAC TATATGTTTT AATTTATATT TTTTCTTTCC
              601 CCCTGGCCTT AACCGAATTT TTTCCCATCG CGATCTAATT CTCCCCCGCT TAATACTGAC
              661 GCTCTCGCAC CCATCTCTCT CCTTCTAGCC TCCGCTAGTC AAAATTTTTG GCGTACTCAC
              721 CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA
              781 GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA GTCCCTGTTC GGGCGCCACT GCTAGAGANT
            841 TT
            //
            Primer: YFP_R (Pr0127)
            Region: EGFP,PGKpro,cPPT_CTS
            Sequence file: RDB12968_A9I4e.seq check
            >12968_12968_A9I4_YFP_R_D02_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCTNANTTGT GGCCGTTTNC GTCGCCGTCC NGCTCGACCA
               61 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGGTG GCGACCGGTA
              121 GCGCTAGGGT CGAAAGGCCC GGAGATGAGG AAGAGGAGAA CAGCGCGGCA GACGTGCGCT
              181 TTTGAAGCGT GCAGAATGCC GGGCCTCCGG AGGACCTTCG GGCGCCCGCC CCGCCCCTGA
              241 GCCCGCCCCT GAGCCCGCCC CCGGACCCAC CCCTTCCCAG CCTCTGAGCC CAGAAAGCGA
              301 AGGAGCAAAG CTGCTATTGG CCGCTGCCCC AAAGGCCTAC CCGCTTCCAT TGCTCAGCGG
              361 TGCTGTCCAT CTGCACGAGA CTAGTGAGAC GTGCTACTTC CATTTGTCAC GTCCTGCACG
              421 ACGCGAGCTG CGGGGCGGGG GGGAACTTCC TGACTAGGGG AGGAGTAGAA GGTGGCGCGA
              481 AGGGGCCACC AAAGAACGGA GCCGGTTGGC GCCTACCGGT GGATGTGGAA TGTGTGCGAG
              541 GCCAGAGGCC ACTTGTGTAG CGCCAAGTGC CCAGCGGGGC TGCTAAAGCG CATGCTCCAG
              601 ACTGCCTTGG GAAAAGCGCC TCCCCTACCC GGTAGAATTC AAAGTGAATT TCTGCTGTCC
              661 CTGTAATAAA CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT
              721 ATGTCTGTTG CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCNNNCT
              781 TTTCTTTTAA AATTGTGGAT GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA
            841 TCTGTCTCTG TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCNGGCCNN
            //
            Primer: BGH_rev2 (Pr0606)
            Region: bGHp(A),3'LTR
            Sequence file: RDB12968_A9I4f.seq check
            >12968_12968_A9I4_3_BGH_rev2_E12_15_ABI24.ab1
                1 NNNNNNNNNN GNNNNNNGGG GGNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG
               61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG
              121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
              181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
              241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC
              301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA
              361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT
              421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT
              481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG
              541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC
              601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT
              661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA
              721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA
            781 CGGTATCGAT GCGGGGNGGC GGNC
            //
            Primer: pAxEF R2 (Pr0059)
            Region: SV40p(A),Zeo,SV40pro_ori
            Sequence file: RDB12968_A9I4g.seq check
            >12968_12968_A9I4_pAxEF R2_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNTNNTTT GTGANNTTNT GNNGCTATTG CTTTATTTGT AACCATTATA
               61 AGCTGCAATA AACAAGTTGG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG
              121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC
              181 GGTGGAATCG AAATCTCGTA GCACGTGTCA GTCCTGCTCC TCGGCCACGA AGTGCACGCA
              241 GTTGCCGGCC GGGTCGCGCA GGGCGAACTC CCGCCCCCAC GGCTGCTCGC CGATCTCGGT
              301 CATGGCCGGC CCGGAGGCGT CCCGGAAGTT CGTGGACACG ACCTCCGACC ACTCGGCGTA
              361 CAGCTCGTCC AGGCCGCGCA CCCACACCCA GGCCAGGGTG TTGTCCGGCA CCACCTGGTC
              421 CTGGACCGCG CTGATGAACA GGGTCACGTC GTCCCGGACC ACACCGGCGA AGTCGTCCTC
              481 CACGAAGTCC CGGGAGAACC CGAGCCGGTC GGTCCAGAAC TCGACCGCTC CGGCGACGTC
              541 GCGCGCGGTG AGCACCGGAA CGGCACTGGT CAACTTGGCC ATGGTTTAGT TCCTCACCTT
              601 GTCGTATTAT ACTATGCCGA TATACTATGC CGATGATTAA TTGTCAACAC GTGCTGATCA
              661 GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA
            721 AAGCCTCCTC ACTACTTCTG GAATAGCTCA GANGCAGAN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl