Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-CDF-PGKG-PRE

Lentivirus vector plasmid of GFP driven by mouse PGK promoter.

Catalog number RDB12968
Resource name CS-CDF-PGKG-PRE
Clone info. Lentivirus vector plasmid of GFP driven by mouse PGK promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB12968 CS-CDF-PGKG-PRE DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB12968z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12968_A9I4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EGFP-M_R
            Sequence file: RDB12968_A9I4a.seq check
            1
            Primer: PRE_rev
            Sequence file: RDB12968_A9I4b.seq check
            1
            Primer: CMV enh_F1
            Sequence file: RDB12968_A9I4c.seq check
            1
            Primer: RRE-M_R
            Sequence file: RDB12968_A9I4d.seq check
            1
            Primer: YFP_R
            Sequence file: RDB12968_A9I4e.seq check
            1
            Primer: BGH_rev2
            Sequence file: RDB12968_A9I4f.seq check
            1
            Primer: pAxEF R2
            Sequence file: RDB12968_A9I4g.seq check
            1

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl