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CSIV-CMV-mc-Myc-IRES2-Venus

Lentivirus vector plasmid of mouse c-Myc with Venus fluorescent protein marker driven by CMV promoter.

Catalog number RDB12953
Resource name CSIV-CMV-mc-Myc-IRES2-Venus
Clone info. Lentivirus vector plasmid of mouse c-Myc with Venus fluorescent protein marker driven by CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-CMV-RfA-IRES2-Venus (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Mouse Myc cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
Mouse Myc

          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
          Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
          Please contact us. We will provide you with appropriate documents.
          Please obtain written approval of Dr. Atsushi Miyawaki.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
          付加的提供条件 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          宮脇敦史博士による提供承諾書 [Word]
          備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          宮脇敦史博士の書面による提供承諾が必要です。
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12953 CSIV-CMV-mc-Myc-IRES2-Venus DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB12953z.seq
          Remarks, protocol and/or map (pdf) RDB12953.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12953_A8Hrp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CSII-CMV5'
          Sequence file: RDB12953_A8Hra.seq check
          >12953_12953_A8Hr_CSII-CMV5'_G08_20_ABI24.ab1
              1 GGGCCGGGCA ATCAAAGGGT TTGGTACAAA AGCAGGCTCC GCGGCCGCCC CCTTCACCAT
             61 GCCCCTCAAC GTGAACTTCA CCAACAGGAA CTATGACCTC GACTACGACT CCGTACAGCC
            121 CTATTTCATC TGCGACGAGG AAGAGAATTT CTATCACCAG CAACAGCAGA GCGAGCTGCA
            181 GCCGCCCGCG CCCAGTGAGG ATATCTGGAA GAAATTCGAG CTGCTTCCCA CCCCGCCCCT
            241 GTCCCCGAGC CGCCGCTCCG GGCTCTGCTC TCCATCCTAT GTTGCGGTCG CTACGTCCTT
            301 CTCCCCAAGG GAAGACGATG ACGGCGGCGG TGGCAACTTC TCCACCGCCG ATCAGCTGGA
            361 GATGATGACC GAGTTACTTG GAGGAGACAT GGTGAACCAG AGCTTCATCT GCGATCCTGA
            421 CGACGAGACC TTCATCAAGA ACATCATCAT CCAGGACTGT ATGTGGAGCG GTTTCTCAGC
            481 CGCTGCCAAG CTGGTCTCGG AGAAGCTGGC CTCCTACCAG GCTGCGCGCA AAGACAGCAC
            541 CAGCCTGAGC CCCGCCCGCG GGCACAGCGT CTGCTCCACC TCCAGCCTGT ACCTGCAGGA
            601 CCTCACCGCC GCCGCGTCCG AGTGCATTGA CCCCTCAGTG GTCTTTCCCT ACCCGCTCAA
            661 CGACAGCAGC TCGCCCAAAT CCTGTACCTC GTCCGATTCC ACGGCCTTCT CTCCTTCCTC
            721 GGACTCGCTG CTGTCCTCCG AGTCCTCCCC ACGGGCCAGC CCTGAGCCCC TAGTGCTGCA
            781 TGAGGAGACA CCGCCCACCA CCAGCAGCGA CTCTGAAGAA GAGCAAGAAG ATGAGGAAGA
            841 AATTGATGTG GTGTCTGGTG GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
            901 TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
            961 CACGTCTCCA CTCACCAGCA CAAACTACGC CGCACCCCCC CTCCACAAGG ATGACTATCC
           1021 AGCTGGCCCA AGAGGGCCAA GTTTGGGACA GTGGCAGGCT CTGAGTCAGA TCAAGCTAAC
          1081 ACCGCAGTTG CTCAAGTCCC AAGATCTCAG ACAACGGGAA GGTCA
          //
          Primer: Myc-1169F
          Sequence file: RDB12953_A8Hrb.seq check
          >12953_12953_A8Hr_Myc-1169F_H08_23_ABI24.ab1
              1 GTACGGAAAG CCCCCAAGGT AGTGATCCTC AAAAAGCCAC CGCCTACATC CTGTCCATTC
             61 AAGCAGACGA GCACAAGCTC ACCTCTGAAA AGGACTTATT GAGGAAACGA CGAGAACAGT
            121 TGAAACACAA ACTCGAACAG CTTCGAAACT CTGGTGCATA AAAGGGTGGG CGCGCCGACC
            181 CAGCTTTCTT GTACAAAGTG GTGATGCTAC CGGTCTCGAG AATTCTGCAG CGGCCGCGGA
            241 TCCGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG GAATAAGGCC
            301 GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTGGC AATGTGAGGG
            361 CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC CCTCTCGCCA
            421 AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA GCTTCTTGAA
            481 GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT GGCGACAGGT
            541 GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA CAACCCCAGT
            601 GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA AGCGTATTCA
            661 ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGATCTGAT CTGGGGCCTC
            721 GGTACACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC
            781 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGGTGAGC
            841 AAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
            901 AAACGGGCCA CAAGTTTCAG CGTGTCCCGG CGAGGGGCGA GGGCGATGCC ACCTACGGCA
            961 GCTGGACCCT GAAGCTGATC TGCACACAGC CAGCCTGGCC GTGCCTGGCC CACCGTCGTG
           1021 AACCACCTTG GGCCTACCGC CTGCAATGCT TCGCCGGCTA ACCCGACACA TGAAGCAGAC
          1081 ACGACTCCAT CCAGCTTCCG TCCA
          //
          Primer: HIVgp_R1
          Sequence file: RDB12953_A8Hrc.seq check
          >12953_12953_A8Hr_HIVgp_R1_A09_03_ABI24.ab1
              1 CGGGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
             61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
            121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
            181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
            241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
            301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
            361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
            421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
            481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
            541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
            601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
            661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
            721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
            781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
            841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA
            901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTTG GAAAGTCCCG
            961 TTGATTTTGG GTGCCCAAAA CAAACTCCCA TTGACGTCAA TGGGGGTGGA GACTTGGAAA
           1021 TCCCCGTGAG GTCAAACCGC TATCCACGCC CATTGAATGG TACTGCCCAA AACGCATCAC
           1081 CATTGCTATT AGCCGAATGA ACTTAATTAC GGTAGAATGT TACCTTGCCC AAGTTAACGA
          //
          Primer: BGH_rev2
          Sequence file: RDB12953_A8Hrd.seq check
          >12953_12953_A8Hr_BGH_rev2_E09_15_ABI24.ab1
              1 CTTGTCGCGG TAGTAGCACG GGGGAGGGGC AAGCAACAGA TGGCTGGCAG ACTAGAAGGC
             61 ACAGTGCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
            121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
            181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
            241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA ATAACTTCGT
            301 ATAATGTATG CTATACGAAG TTATGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC
            361 CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG
            421 TCTTAAAGGT ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG
            481 CCAGGCACAA TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC
            541 TAGGTCTCGA CGGTATCGAT GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG
            601 GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC
            661 CGCTGGATTG AGGGCCGAAG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC
            721 AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACAACACCA CGGAATTGTC
            781 AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA GCGGGCAAGG CAGGCGGCGA TGAGTTCCGC
            841 CGTGGCAATA GGGAGGGGGA AAGCGAAAGT CCCGGAAAGG AGCTGACAGG TGGTGGCAAT
            901 GCCCCAACCA GTGGGGGGTT GCGTCAGCAA ACACAGTGCA CACCACGCCA CGTTTGCCTG
            961 ACAACGGGCC ACAACTCCTC ATAAGAGACA GCACCCAGGA TTTATACACG AGAGAAAATG
           1021 AAGCCCATAC GGCAGCATAG CATGATACAA GGGCAATTAA GGCAGCGTAT CACATAGCCG
          1081 TAAAGAGCAC TAGTTAGATA CAGTCAATCC TTTCAACAAA TTTG
          //
          Primer: IRES-F
          Sequence file: RDB12953_A8Hre.seq check
          >12953_12953_A8Hr_IRES-F_D09_12_ABI24.ab1
              1 CATCTTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
             61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG
            121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
            181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
            241 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC
            301 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
            361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
            421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
            481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
            541 AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC
            601 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
            661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC
            721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
            781 GCCGCCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAGAATTTC AAGGCCTCTC
            841 GAGCCTCTAG AGTTAACATC GAGGGATCAA GCTTATCGAT ATCAACCTCT GGATACAAAA
            901 TTGTGAAAGA TTGACTGGTA TCTACTATGT TGCTCTTTTA CGCTATGTGG ATACGCTGCT
            961 TAATGCCTTT GTATCATGCT ATTGCTCCGT ATGCATCAAT TTCTCTCTTG ATAATCCTGG
          1021 ATGCTGACTC TTTAGAGGGA TGAGCCGTTG TCAGCAACGG TGGCCGT
          //
          Primer: SV40pro_F
          Sequence file: RDB12953_A8Hrf.seq check
          >12953_12953_A8Hr_SV40pro_F_F09_18_ABI24.ab1
              1 GCTGGTCCTG GACATCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
            121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
            181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
            241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
            301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
            361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
            421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
            481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
            541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
            601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
            661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
            721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
            781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
            841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
            901 ACGAGCCGGA AAGCATAAAA GTGTAAAGCC TTGGGGTGCC TAATGAGTGA GCTAACTCAC
            961 ATTTAATTTG CGTTTGCGCT CACTGCCCGC CTTTCCAGTC GGGAAACCCT TGTCGTGCAG
           1021 CCTGCATTTA ATGAATTCGG TCACGCGCGG GAGGAGGCGG TTGCGTAATG GGCGCCTCTT
          1081 TCGCATTCTT CGGACTCA
          //
          Primer:
          Sequence file: RDB12953_A8Hrg.seq check
          1
          Primer:
          Sequence file: RDB12953_A8Hrh.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl