Resource data sheet
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CSIV-CMV-mc-Myc-IRES2-Venus

Lentivirus vector construct of mouse c-Myc with Venus fluorescent protein marker driven by CMV promoter.

Catalog number RDB12953
Resource name CSIV-CMV-mc-Myc-IRES2-Venus
Clone info. Lentivirus vector construct of mouse c-Myc with Venus fluorescent protein marker driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-CMV-RfA-IRES2-Venus (Plasmid)
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Mouse Myc cDNA
Originator Miyoshi, Hiroyuki |
 
Other clones in our bank mouse Myc (NCBI Gene 17869) |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12953 CSIV-CMV-mc-Myc-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12953z.seq from Depositor
Electronic file PDF RDB12953.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12953_A8Hrp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' RDB12953_A8Hra.seq
>12953_12953_A8Hr_CSII-CMV5'_G08_20_ABI24.ab1
    1 GGGCCGGGCA ATCAAAGGGT TTGGTACAAA AGCAGGCTCC GCGGCCGCCC CCTTCACCAT
   61 GCCCCTCAAC GTGAACTTCA CCAACAGGAA CTATGACCTC GACTACGACT CCGTACAGCC
  121 CTATTTCATC TGCGACGAGG AAGAGAATTT CTATCACCAG CAACAGCAGA GCGAGCTGCA
  181 GCCGCCCGCG CCCAGTGAGG ATATCTGGAA GAAATTCGAG CTGCTTCCCA CCCCGCCCCT
  241 GTCCCCGAGC CGCCGCTCCG GGCTCTGCTC TCCATCCTAT GTTGCGGTCG CTACGTCCTT
  301 CTCCCCAAGG GAAGACGATG ACGGCGGCGG TGGCAACTTC TCCACCGCCG ATCAGCTGGA
  361 GATGATGACC GAGTTACTTG GAGGAGACAT GGTGAACCAG AGCTTCATCT GCGATCCTGA
  421 CGACGAGACC TTCATCAAGA ACATCATCAT CCAGGACTGT ATGTGGAGCG GTTTCTCAGC
  481 CGCTGCCAAG CTGGTCTCGG AGAAGCTGGC CTCCTACCAG GCTGCGCGCA AAGACAGCAC
  541 CAGCCTGAGC CCCGCCCGCG GGCACAGCGT CTGCTCCACC TCCAGCCTGT ACCTGCAGGA
  601 CCTCACCGCC GCCGCGTCCG AGTGCATTGA CCCCTCAGTG GTCTTTCCCT ACCCGCTCAA
  661 CGACAGCAGC TCGCCCAAAT CCTGTACCTC GTCCGATTCC ACGGCCTTCT CTCCTTCCTC
  721 GGACTCGCTG CTGTCCTCCG AGTCCTCCCC ACGGGCCAGC CCTGAGCCCC TAGTGCTGCA
  781 TGAGGAGACA CCGCCCACCA CCAGCAGCGA CTCTGAAGAA GAGCAAGAAG ATGAGGAAGA
  841 AATTGATGTG GTGTCTGGTG GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
  901 TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
  961 CACGTCTCCA CTCACCAGCA CAAACTACGC CGCACCCCCC CTCCACAAGG ATGACTATCC
 1021 AGCTGGCCCA AGAGGGCCAA GTTTGGGACA GTGGCAGGCT CTGAGTCAGA TCAAGCTAAC
1081 ACCGCAGTTG CTCAAGTCCC AAGATCTCAG ACAACGGGAA GGTCA
//
Primer: Myc-1169F RDB12953_A8Hrb.seq
>12953_12953_A8Hr_Myc-1169F_H08_23_ABI24.ab1
    1 GTACGGAAAG CCCCCAAGGT AGTGATCCTC AAAAAGCCAC CGCCTACATC CTGTCCATTC
   61 AAGCAGACGA GCACAAGCTC ACCTCTGAAA AGGACTTATT GAGGAAACGA CGAGAACAGT
  121 TGAAACACAA ACTCGAACAG CTTCGAAACT CTGGTGCATA AAAGGGTGGG CGCGCCGACC
  181 CAGCTTTCTT GTACAAAGTG GTGATGCTAC CGGTCTCGAG AATTCTGCAG CGGCCGCGGA
  241 TCCGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG GAATAAGGCC
  301 GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTGGC AATGTGAGGG
  361 CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC CCTCTCGCCA
  421 AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA GCTTCTTGAA
  481 GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT GGCGACAGGT
  541 GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA CAACCCCAGT
  601 GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA AGCGTATTCA
  661 ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGATCTGAT CTGGGGCCTC
  721 GGTACACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC
  781 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGGTGAGC
  841 AAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  901 AAACGGGCCA CAAGTTTCAG CGTGTCCCGG CGAGGGGCGA GGGCGATGCC ACCTACGGCA
  961 GCTGGACCCT GAAGCTGATC TGCACACAGC CAGCCTGGCC GTGCCTGGCC CACCGTCGTG
 1021 AACCACCTTG GGCCTACCGC CTGCAATGCT TCGCCGGCTA ACCCGACACA TGAAGCAGAC
1081 ACGACTCCAT CCAGCTTCCG TCCA
//
Primer: HIVgp_R1 RDB12953_A8Hrc.seq
>12953_12953_A8Hr_HIVgp_R1_A09_03_ABI24.ab1
    1 CGGGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
  181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
  241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
  301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
  361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
  421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
  481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
  541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
  601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
  661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
  721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA
  901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTTG GAAAGTCCCG
  961 TTGATTTTGG GTGCCCAAAA CAAACTCCCA TTGACGTCAA TGGGGGTGGA GACTTGGAAA
 1021 TCCCCGTGAG GTCAAACCGC TATCCACGCC CATTGAATGG TACTGCCCAA AACGCATCAC
 1081 CATTGCTATT AGCCGAATGA ACTTAATTAC GGTAGAATGT TACCTTGCCC AAGTTAACGA
//
Primer: BGH_rev2 RDB12953_A8Hrd.seq
>12953_12953_A8Hr_BGH_rev2_E09_15_ABI24.ab1
    1 CTTGTCGCGG TAGTAGCACG GGGGAGGGGC AAGCAACAGA TGGCTGGCAG ACTAGAAGGC
   61 ACAGTGCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA ATAACTTCGT
  301 ATAATGTATG CTATACGAAG TTATGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC
  361 CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG
  421 TCTTAAAGGT ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG
  481 CCAGGCACAA TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC
  541 TAGGTCTCGA CGGTATCGAT GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG
  601 GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC
  661 CGCTGGATTG AGGGCCGAAG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC
  721 AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACAACACCA CGGAATTGTC
  781 AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA GCGGGCAAGG CAGGCGGCGA TGAGTTCCGC
  841 CGTGGCAATA GGGAGGGGGA AAGCGAAAGT CCCGGAAAGG AGCTGACAGG TGGTGGCAAT
  901 GCCCCAACCA GTGGGGGGTT GCGTCAGCAA ACACAGTGCA CACCACGCCA CGTTTGCCTG
  961 ACAACGGGCC ACAACTCCTC ATAAGAGACA GCACCCAGGA TTTATACACG AGAGAAAATG
 1021 AAGCCCATAC GGCAGCATAG CATGATACAA GGGCAATTAA GGCAGCGTAT CACATAGCCG
1081 TAAAGAGCAC TAGTTAGATA CAGTCAATCC TTTCAACAAA TTTG
//
Primer: IRES-F RDB12953_A8Hre.seq
>12953_12953_A8Hr_IRES-F_D09_12_ABI24.ab1
    1 CATCTTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG
  121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
  181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
  241 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC
  301 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
  361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
  421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
  481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
  541 AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC
  601 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
  661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC
  721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
  781 GCCGCCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAGAATTTC AAGGCCTCTC
  841 GAGCCTCTAG AGTTAACATC GAGGGATCAA GCTTATCGAT ATCAACCTCT GGATACAAAA
  901 TTGTGAAAGA TTGACTGGTA TCTACTATGT TGCTCTTTTA CGCTATGTGG ATACGCTGCT
  961 TAATGCCTTT GTATCATGCT ATTGCTCCGT ATGCATCAAT TTCTCTCTTG ATAATCCTGG
1021 ATGCTGACTC TTTAGAGGGA TGAGCCGTTG TCAGCAACGG TGGCCGT
//
Primer: SV40pro_F RDB12953_A8Hrf.seq
>12953_12953_A8Hr_SV40pro_F_F09_18_ABI24.ab1
    1 GCTGGTCCTG GACATCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AAGCATAAAA GTGTAAAGCC TTGGGGTGCC TAATGAGTGA GCTAACTCAC
  961 ATTTAATTTG CGTTTGCGCT CACTGCCCGC CTTTCCAGTC GGGAAACCCT TGTCGTGCAG
 1021 CCTGCATTTA ATGAATTCGG TCACGCGCGG GAGGAGGCGG TTGCGTAATG GGCGCCTCTT
1081 TCGCATTCTT CGGACTCA
//
Primer: RDB12953_A8Hrg.seq
//
Primer: RDB12953_A8Hrh.seq
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.09

GNP_filter3_RDBDEP_html_180823.pl