Resource data sheet
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CS-hp53-shRNA-CG

Lentivirus vector construct of shRNA targeting human TP53 with GFP marker.

Catalog number RDB12946
Resource name CS-hp53-shRNA-CG
Clone info. Lentivirus vector construct of shRNA targeting human TP53 with GFP marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human TP53 cDNA
Originator Miyoshi, Hiroyuki |
 
Other clones in our bank human TP53 (NCBI Gene 7157) |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
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Material Transfer Agreement (MTA) [Word]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12946 CS-hp53-shRNA-CG DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12946z.seq from Depositor
Electronic file PDF RDB12946.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12946_A4K5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_v2_F RDB12946_A4K5a.seq
>12946_12946_A4K5_2_cPPT+CTS_v2_F_B09_06.ab1
    1 TCCTAGGAAA TCGGACTAAA GAATTACAAA AACAAATTAC AAAAAATTCA AAATTTTCGG
   61 GTTTATTACA GGGACAGCAG AAATTCACTT TGAATTATCA CAAGTTTGTA CAAAAAAGCA
  121 GGCTCCACCA TGGGAACCAA TTCAGTCGAC TGGATCCGGT ACCGAATTCG AACGCTGACG
  181 TCATCAACCC GCTCCAAGGA ATCGCGGGCC CAGTGTCACT AGGCGGGAAC ACCCAGCGCG
  241 CGTGCGCCCT GGCAGGAAGA TGGCTGTGAG GGACAGGGGA GTGGCGCCCT GCAATATTTG
  301 CATGTCGCTA TGTGTTCTGG GAAATCACCA TAAACGTGAA ATGTCTTTGG ATTTGGGAAT
  361 CTTATAAGTC CCTATCAGTG ATAGAGATCC CCACTCTAGT GGTGATTTAC TACGTGTGCT
  421 GTCCGTAGTA GATTACCACT GGAGTTTTTT GGAAATCTAG ACCCAGCTTT CTTGTACAAA
  481 GTGGTGATAA TTCGATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT
  541 GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC
  601 CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA
  661 TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA
  721 TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA
  781 TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT
  841 CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA
  901 CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA
  961 AAATCAACGG GACTTTCCAA AAATGTCGTA ACAACTCCGC CCCAATTGAC GCAAAATGGG
 1021 CGGTAGGCGT GTACGGGTGG GGAGGTCTAA TATAAAGCAG AGCTGGGTTT TAGTGAAACG
 1081 TTCAGATCCG CTAGCGCTTA CGATTCGCAT CATGATTGAG CCAAGGACGG AGGAAGCTGT
1141 TTCAACCCGG GGGGTTGACG
//
Primer: PRE_rev RDB12946_A4K5b.seq
>12946_12946_A4K5_2_PRE_rev_C09_09.ab1
    1 CGGGATTCAA ATAGCGTAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGAGATCCTA CTAGTAGGAT
  121 CTGAGTCCGG ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC
  181 TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGGTG
  241 CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG
  301 TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTCACC
  361 TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT GTAGTTGTAC
  421 TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG
  481 CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG
  541 TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG
  601 TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG
  661 GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC
  721 AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT
  781 ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG
  841 CTCACCATGG TGGCGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA CCAGCTCTGC
  901 TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGGAGTTG
  961 TTACGACATT TTGGGAAAGT CCCGTTTGAT TTTGGGTGCC CAAAACAAAC TCCCCATTGA
 1021 CGTCATGGGG TGAGACTGTA ATCCCCCGTG AGTCAACCGC TATCCACGCC CAATGAATGT
1081 ACTGCCTAAG CGCATCACAT GATAATAGCG AATGAACTAA TTACGTAGAA ATGGTTAC
//
Primer: HIVgp_R1 RDB12946_A4K5c.seq
>12946_12946_A4K5_HIVgp_R1_2_A01_01.ab1
    1 CCGGCCTCCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGCTTGCT GTGCGGTGGT
  181 CTTACTTTTG TTTTGCTCTT CCTCTATCTT GTCTAAAGCT TCCTTGGTGT CTTTTATCTC
  241 TATCCTTTGA TGCACACAAT AGAGGGTTGC TACTGTATTA TATAATGATC TAAGTTCTTC
  301 TGATCCTGTC TGAAGGGATG GTTGTAGCTG TCCCAGTATT TGTCTACAGC CTTCTGATGT
  361 TTCTAACAGG CCAGGATTAA CTGCGAATCG TTCTAGCTCC CTGCTTGCCC ATACTATATG
  421 TTTTAATTTA TATTTTTTCT TTCCCCCTGG CCTTAACCGA ATTTTTTCCC ATCGCGATCT
  481 AATTCTCCCC CGCTTAATAC TGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT
  541 AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC
  601 AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT TCAAGTCCCT
  661 GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
  721 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
  781 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
  841 AACCAGAGAG ACCCAGTACA GGCAAAACGC GCTGCTTATA TAGACCTCCC ACCGTACACG
  901 CCTACCGCCC ATTTGCGTCA ATGGGGGCGG AGTTGTTACG ACATTTTGGA AAGTCCCGTT
  961 GATTTTGGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTGGGAAATC
 1021 CCCGTGAGTC AACCCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC ATCACCCATG
 1081 GTAATAGCCG GATGACTATA CGGTAGATGT ACTGCCAAGG TAGAAAGTCC CATAAGTCAT
1141 GTACTTGGGC ATAATGCCAG GGCGGGGCCC AATTTTAACC CGGTTCCA
//
Primer: BGH_rev2 RDB12946_A4K5d.seq
>12946_12946_A4K5_2_BGHrev2_D09_12.ab1
    1 CATTTGGTTC AAGAGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
  121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
  181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
  241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
  301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
  361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
  421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
  481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
  541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
  601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
  661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
  721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
  781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC
  841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAAG GTCCGCTGGA
  901 TTGAGGGCCG AAAGGGACGT AGCAGAACGA CGTCCCGCGC AGAATCCAGG TGGCAACACA
  961 GGCGAGCAGC CATGGGAAAG GGACGTCAGC TTCCCCGACA CACCCACGGG AATGTCAGTG
 1021 CCCACAGCCG AGCCCCTGTC AGCAGCGGGC AAGCAGGCGC CGATGAGTCG GCGTGTCATT
1081 AGAGGGGGAA AGCGAAGTTC CTGCAAGGAG CTGAACAAGG TTGGGGTGGG CCA
//
Primer: SV40pro_F RDB12946_A4K5e.seq
>RDB12946_12946_A4K5_SV40pro_F_2_H12_24.ab1
    1 CCCTGTCCTT GCTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
  961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA
 1021 ATGAATCGGC CAACGCGCGG GGAGAGGCGG GTTTGCGTAT TGGCGCTCTT CCGCTTTCCT
 1081 CGCTCACTGA CTCGCTGCGC TCGGGTCGTT TCGGCTGCGG CGAGCGTATC AGCTCACTCA
 1141 AAAGCGTATA CGGCTATCCA CAGGAATCAG GATTACGCGA AGCAAAGGAA ATCCAGTAGT
1201 CCGTGGC
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.09

GNP_filter3_RDBDEP_html_180823.pl