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CS-UbC-hSox2-IRES2-Venus (#RDB12915)

Lentivirus vector plasmid of human SOX2 for generating iPS cells.

Clone info. Lentivirus vector plasmid of human SOX2 for generating iPS cells.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CS-UbC-RfA-IRES2-Venus (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Human SOX2 cDNA
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB12915z.seq
Remarks, protocol and/or map (pdf) RDB12915.pdf

Distribution information

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12915 CS-UbC-hSox2-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-UbC-hSox2-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12915).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12915_A4Jfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: UbCpro-F ()
Sequence file: RDB12915_A4Jfa.seq check
>12915_12915_A4Jf_2_UbCpro-F_F05_17_ABI24.ab1
    1 TGCATGGTAG TTCAGTGTTA GACTAGTAAA TTGTCCGCTA AATTCTGGCC GTTTTTGGCT
   61 TTTTTGTTAG ACCGGACCGT GTTTAGCGCT ACCGGTCTCG AGAATTCTGC AGCGGCCATC
  121 ACAAGTTTGT ACAAAAAAGC AGGCTCCGCG GCCGCCCCCT TCACCATGTA CAACATGATG
  181 GAGACGGAGC TGAAGCCGCC GGGCCCGCAG CAAACTTCGG GGGGCGGCGG CGGCAACTCC
  241 ACCGCGGCGG CGGCCGGCGG CAACCAGAAA AACAGCCCGG ACCGCGTCAA GCGGCCCATG
  301 AATGCCTTCA TGGTGTGGTC CCGCGGGCAG CGGCGCAAGA TGGCCCAGGA GAACCCCAAG
  361 ATGCACAACT CGGAGATCAG CAAGCGCCTG GGCGCCGAGT GGAAACTTTT GTCGGAGACG
  421 GAGAAGCGGC CGTTCATCGA CGAGGCTAAG CGGCTGCGAG CGCTGCACAT GAAGGAGCAC
  481 CCGGATTATA AATACCGGCC CCGGCGGAAA ACCAAGACGC TCATGAAGAA GGATAAGTAC
  541 ACGCTGCCCG GCGGGCTGCT GGCCCCCGGC GGCAATAGCA TGGCGAGCGG GGTCGGGGTG
  601 GGCGCCGGCC TGGGCGCGGG CGTGAACCAG CGCATGGACA GTTACGCGCA CATGAACGGC
  661 TGGAGCAACG GCAGCTACAG CATGATGCAG GACCAGCTGG GCTACCCGCA GCACCCGGGC
  721 CTCAATGCGC ACGGCGCAGC GCAGATGCAG CCCATGCACC GCTACGACGT GAGCGCCCTG
  781 CAGTACAACT CCATGACCAG CTCGCAGACC TACATGAACG GCTCGCCCAC CTACAGCATG
  841 TCCTACTCGC AGCAGGCACC CCTGGCATGG CTCTTGGCTC CATGGGTTCG GTGGTCAAGT
  901 CCGAGGCCAG CTCCAGCCCC CCTGTGGTTA CCTCTCCTCC CACTCCATGG CGCCCTGCAG
  961 GCCGGGACTC TGGACATGAT CAGCATGTAT CTCCCGCGCG AGTGCTGACC GGCCGCCCCC
 1021 AGCAGACTCA CATGTCCCAG CACTTACCGA GCGATGATGC TGTCGCATAC GGTCACACTG
1081 CCGTTCACAT GACAAGGCTG TGGC
//
Primer: IRES_F ()
Sequence file: RDB12915_A4Jfb.seq check
>12915_12915_A4Jf_IRES_F_2_H08_23.ab1
    1 GCAGGCTTAA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTGA GCAAGGGCGA
  121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
  181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
  241 GCTGATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGGG
  301 CTACGGCCTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
  361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
  421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
  481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
  541 CAACAGCCAC AACGTCTATA TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT
  601 CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC GCCGACCACT ACCAGCAGAA
  661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCTACCAGTC
  721 CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC
  781 CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAGAATTCA AGGCCTCTCG
  841 AGCCTCTAGA GTTAACATCG AGGGATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA
  901 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC
  961 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC
 1021 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGCAACGTG
 1081 GCGTGGTGTG CACTGTGTTG CTGACGCAAC CCCCACTGGT TGGGGGCATT GCAACCACCT
1141 GTCAGCTCTT CCAGACCTTT CGCTTTCCCC CCTCCCCTAT TGGTCAATCA GA
//
Primer: IRES_R ()
Sequence file: RDB12915_A4Jfc.seq check
>12915_12915_A4Jf_IRES_R_2_E06_15.ab1
    1 GGCGAAAGCG GCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG
   61 CCATCACCAC TTTGTACAAG AAAGCTGGGT CGGCGCGCCC ACCCTTTCAC ATGTGTGAGA
  121 GGGGCAGTGT GCCGTTAATG GCCGTGCCGG GCACCGGGCC GCTCTGGTAG TGCTGGGACA
  181 TGTGAAGTCT GCTGGGGGCG GCGGGTTCCG GCACCTCGGC GCCGGGGAGA TACATGCTGA
  241 TCATGTCCCG GAGGTCCCCG GCCTGGCAGG GCGCCCTGGA GTGGGAGGAA GAGGTAACCA
  301 CAGGGGGGCT GGAGCTGGCC TCGGACTTGA CCACCGAACC CATGGAGCCA AGAGCCATGC
  361 CAGGGGTGCC CTGCTGCGAG TAGGACATGC TGTAGGTGGG CGAGCCGTTC ATGTAGGTCT
  421 GCGAGCTGGT CATGGAGTTG TACTGCAGGG CGCTCACGTC GTAGCGGTGC ATGGGCTGCA
  481 TCTGCGCTGC GCCGTGCGCA TTGAGGCCCG GGTGCTGCGG GTAGCCCAGC TGGTCCTGCA
  541 TCATGCTGTA GCTGCCGTTG CTCCAGCCGT TCATGTGCGC GTAACTGTCC ATGCGCTGGT
  601 TCACGCCCGC GCCCAGGCCG GCGCCCACCC CGACCCCGCT CGCCATGCTA TTGCCGCCGG
  661 GGGCCAGCAG CCCGCCGGGC AGCGTGTACT TATCCTTCTT CATGAGCGTC TTGGTTTTCC
  721 GCCGGGGCCG GTATTTATAA TCCGGGTGCT CCTTCATGTG CAGCGCTCGC AGCCGCTTAG
  781 CCTCGTCGAT GAACGGCCGC TTCTCCGTCT CCGACAAAAG TTTCCACTCG GCGCCCAGGC
  841 GCTTGCTGAT CTCCGAGTTG TGCATCTTGG GGTTCTCCTG GGCCATCTTG CGCCGCTGCC
  901 CGCGGGACCA CACCATGAAG GCATTCATGG GCCGCTTGAC GCGGTCCGGG CTGTTTTTCT
  961 GGTTGCCGCC GGCCGCCGCC GCGGTGGAGT TGCCGCCGCC GCCCCCCGAA GTTTGCTGCG
 1021 GGCCCGGCGG CTTCAGCTCG TCTCATCATG TGTACATGGT GAAGGGGCGC CGCGGAGCTG
 1081 CTTTTTTTGT ACAAACCTGT GATGACGGCT GCAGCTCTCG AGACTGTTAG CGCTAACACG
1141 ATTCGGGTCT ACATAAGCCT AAGAACCGGC AGACTTAGTC GAACAAGT
//
Primer: CMV_Forward ()
Sequence file: RDB12915_A4Jfd.seq check
>12915_12915_A4Jf_CMV_Forward_2_H05_23.ab1
    1 TTGTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GCGGCCGCTG
  781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG
  901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA
  961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTATTGTCT
 1021 GGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGGCTATT GAGGCGCAAC AGCATCTGTG
 1081 CAACTCACAG TCTGGGGGCA ATCAAGGCAG CTCAGCAAGA ATCCTGATCT GTGAAAGAAA
1141 TACCTTAAAG GAAATCAACA AGGCCTCTCC CTTTG
//
Primer: UbC-N-R ()
Sequence file: RDB12915_A4Jfe.seq check
>12915_12915_A4Jf_2_UbC-N-R_E05_14_ABI24.ab1
    1 CAGGACAGCG GGCGCTGTCT GAGCGTCCGG GCGGAGGATC AGGACGCTCG CTGCGCCCTT
   61 CGTCTGACGT GGCAGCGCTC GCCGTGAGGA GGGGGGCGCC CGCGGGGGGC GCCAAAACCC
  121 GGCGCGGAGG CCAGATCTCC CGATCTCGAG CTAGAATTCA AAGTGAATTT CTGCTGTCCC
  181 TGTAATAAAC CCGAAAATTT TGAATTTTTG TAATTTGTTT TTGTAATTCT TTAGTTTGTA
  241 TGTCTGTTGC TATTATGTCT ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT
  301 TTCTTTTAAA ATTGTGGATG AATACTGCCA TTTGTAGATC CGTTCACTAA TCGAATGGAT
  361 CTGTCTCTGT CTCTCTCTCC ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC
  421 GGGGTTGGGA GGTGGGTCTG AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT
  481 ATAGAAAGTA CAGCAAAAAC TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAT
  541 AATTTTATAT ACCACAGCCA ATTTGTTATG TTAAACCAAT TCCACAAACT TGCCCATTTA
  601 TCTAATTCCA ATAATTCTTG TTCATTCTTT TCTTGCTGGT TTTGCGATTC TTCAATTAAG
  661 GAGTGTATTA AGCTTGTGTA ATTGTTAATT TCTCTGTCCC ACTCCATCCA GGTCGTGTGA
  721 TTCCAAATCT GTTCCAGAGA TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG
  781 CAAATGAGTT TTCCAGAGCA ACCCCAAATC CCCAGGAGCT GTTGATCCTT TAGGTATCTT
  841 TCCACAGCCA GGATTCTTGC CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA
  901 TGCTGTTGCG CCTCATAGCC CTCAGCAAAT TGTTCTGCTG CTGCACTATA CCAGACATAT
  961 TGTCTGGCCT GTACGTCAGC GTCATTGACG CTGCGCCCAT AGTGCTTCTG CTGCTTCCAG
 1021 ACCCAAGACA AGCTTCTATT CCCACTGCTC GTTACCCTCT GGCACAATCT CCTAGCCTGA
1081 TTGGATGCTA CTCGTAAGTG ATTCCAAATA T
//
Primer: BGH_rev ()
Sequence file: RDB12915_A4Jff.seq check
>12915_12915_A4Jf_BGH_rev_2_C06_09.ab1
    1 CATTATTACG GGGTCTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
   61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA
  241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC
  301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT
  361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG
  421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA
  481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT
  541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG
  601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG
  661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG
  721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC
  781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT
  841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG
  901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC
  961 AGCAGCGGGC AAGGCAGGCG GCGATGAGTT CCGCCGTGGC AATAGGGAGG GGGAAAGCGA
 1021 AAGTCCCGGA AAGGAGCTGA CAGTGTGGCA ATGCCCCACC AGTGGGGTTG CGTCAGCAAC
 1081 ACAGTGCACA CCACGCCACG TTGCTGACAC GGCACAACTC TCATAGGAAC AGCAACAGGA
1141 TTTATCATGA GGAGAAAATG GAAAGGCCAT ACGGCAGCCA AATAGCCATC ATC
//
Primer: SV40pro_F ()
Sequence file: RDB12915_A4Jfg.seq check
>12915_12915_A4Jf_SV40pro_F_2_D06_12.ab1
    1 CCCCCGGCCC CTTTACATTT CAGAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAGCACG TGTTGACAAT
  121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG
  181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
  241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
  301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
  361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
  421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
  481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
  541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
  601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
  661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
  721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
  781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG
  841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC
  901 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC
  961 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG
 1021 CATTAATGAA TCGGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTTCCG
 1081 CTTCTCGCTC ACTGACTCGC TGGCGCTCGG TCGTTCGGCT GGCGCGAGCG TATCAGGCCT
1141 CACTCAAAGG CGGTATACCG GTTATTCCCA CAGAAATTTC AGGGGATT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Takahashi, Y., Organoid-derived intestinal epithelial cells are a suitable model for preclinical toxicology and pharmacokinetic studies. iScience 25 (7): 104542 (2022). PMID 35754737. [link to RRC of NBRP]