Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-hKlf4-IRES2-Venus

Lentivirus vector plasmid of human KLF4.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Please obtain written approval of Dr. Atsushi Miyawaki. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 付加的提供条件： 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 宮脇敦史博士による提供承諾書 [Word]
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 宮脇敦史博士の書面による提供承諾が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12910_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 Sequence file: RDB12910_A4Jfa.seq
>12910_12910_A4Jf_pAxEF_F2_1_H03_24.ab1     1 ATCTCATTTC AGTGTCGTGA CACGCTACCG GTCTCGAGAA TTCTGCAGCG GCCATCACAA    61 GTTTGTACAA AAAAGCAGGC TCCGCGGCCG CCCCCTTCAC CATGGCTGTC AGTGACGCGC   121 TGCTCCCATC TTTCTCCACG TTCGCGTCTG GCCCGGCGGG AAGGGAGAAG ACACTGCGTC   181 AAGCAGGTGC CCCGAATAAC CGCTGGCGGG AGGAGCTCTC CCACATGAAG CGACTTCCCC   241 CAGTGCTTCC CGGCCGCCCC TATGACCTGG CGGCGGCGAC CGTGGCCACA GACCTGGAGA   301 GCGGCGGAGC CGGTGCGGCT TGCGGCGGTA GCAACCTGGC GCCCCTACCT CGGAGAGAGA   361 CCGAGGAGTT CAACGATCTC CTGGACCTGG ACTTTATTCT CTCCAATTCG CTGACCCATC   421 CTCCGGAGTC AGTGGCCGCC ACCGTGTCCT CGTCAGCGTC AGCCTCCTCT TCGTCGTCGC   481 CGTCGAGCAG CGGCCCTGCC AGCGCGCCCT CCACCTGCAG CTTCACCTAT CCGATCCGGG   541 CCGGGAACGA CCCGGGCGTG GCGCCGGGCG GCACGGGCGG AGGCCTCCTC TATGGCAGGG   601 AGTCCGCTCC CCCTCCGACG GCTCCCTTCA ACCTGGCGGA CATCAACGAC GTGAGCCCCT   661 CGGGCGGCTT CGTGGCCGAG CTCCTGCGGC CAGAATTGGA CCCGGTGTAC ATTCCGCCGC   721 AGCAGCCGCA GCCGCCAGGT GGCGGGCTGA TGGGCAAGTT CGTGCTGAAG GCGTCGCTGA   781 GCGCCCCTGG CAGCGAGTAC GGCAGCCCGT CGGTCATCAG CGTCAGCAAA GGCAGCCCTG   841 ACGGCAGCCA CCCGGGTGGT GGTGGCGCCC TACAACGGCG GGCCGCCGCG CACGTGCCCC   901 AAGATCAAGC AGGAGGCGGT CTCTTCGTGC ACCCACTTGG GGCGCTGGAC CCCCTCTCAG   961 CATGACACGG TCGCTGCACA CGACTTCCCC CTTGGGGCGC AGCTCCCCAA GCAAGACTAC  1021 CCCGAACCCT TGGGTCCTGA GAGTGCCTGA CAGCAGACTG TCAGCTGGCG TGCGAATCTC 1081 GGCTTCATTC CACCCAGGCC ATC //
Primer: IRES-R Sequence file: RDB12910_A4Jfb.seq
>12910_12910_A4Jf_IRES_R_1_D04_10.ab1     1 ACAAAACGGG CTTGGCCAGT AACGTTAGGG GGGGGGGAGG GAGAGGGGCG GATCCGCGGC    61 CATCACCACT TTGTACAAGA AAGCTGGGTC GGCGCGCCCA CCCTTTAAAA ATGTCTCTTC   121 ATGTGTAAGG CGAGGTGGTC CGACCTGGAA AATGCTCGGT CGCATTTTTG GCACTGGAAC   181 GGGCGGTGCC CCGTGTGTTT ACGGTAGTGC CTGGTCAGTT CATCTGAGCG GGCGAATTTC   241 CATCCACAGC CGTCCCAGTC ACAGTGGTAA GGTTTCTCAC CTGTGTGGGT TCGCAGGTGT   301 GCCTTGAGAT GGGAACTCTT TGTGTAGGTT TTGCCGCAGC CCGCGTAATC ACAAGTGTGG   361 GTGGCGGTCC TTTTCCGGGG CCACGATCGT CTTCCCCTCT TTGGCTTGGG CTCCTCTGGC   421 ATGCAGGAAC CGGGTGGCAT GAGCTCTTGG TAATGGAGCG GCGGGACTTG CGGCTGCATC   481 TGATCGGGCA GGAAGGATGG GTAATTGGGC CCCGGGTGGG GATGGAAGCC GGGAGGAAGC   541 GGCAGGGCAG GGTGACAGTC CCTGCTGCTC AGCACTTCCT CAAGACCCAG GGTCGGGGTA   601 GTCCTGCTGG GGAGCTGCCG CCCCAGGGGG AAGTCGTGTG CAGCCGGCCG GTGGCCATTG   661 CTGAGAGGGG GTCCAGCGCC CAAGTGGGTG CACGAAGAGA CCGCCTCCTG CTTGATCTTG   721 GGGCACGTGC GCGGCGGCCC GCCGTTGTAG GGCGCCACCA CCACCGGGTG GCTGCCGTCA   781 GGGCTGCCTT TGCTGACGCT GATGACCGAC GGGCTGCCGT ACTCGCTGCC AGGGGCGCTC   841 AGCGACGCCT TCAGCACGAA CTTGCCCATC AGCCCGCCAC CTGGCGGCTG CGGCTGCTGC   901 GGCGGAATGT ACACCGGGTC CAATTCTGGC CGCAGGAGCT CGGCCACGAA GCCGCCCGAG   961 GGGCTCACGT CGTTGATGTC CGCCAGGTTT GAAGGAGCCG TCGGAGGGGG AGCGGGACTC  1021 TCTGCCATAG AGGAGCCTCC GCCGTGCCGC CGGCGCACGC CCGGTCGTTC CCGGCCCGGA  1081 ATCCGATAGT GAGCTGCAGG TGGAGGCGCG CTGGCAGGAC GCTGCTCGAC GCGACGACGA 1141 GAGGAAGGCC TGACGCCTTG ACA //
Primer: IRES-F Sequence file: RDB12910_A4Jfc.seq
>12910_12910_A4Jf_IRES_F_1_A04_01.ab1     1 GCATCTTTAA TGTGTTTAGT CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA    61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGTG AGCAAGGGCG   121 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC   181 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA   241 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG   301 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA   361 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA   421 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC   481 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT   541 ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT   601 TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA   661 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT   721 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA   781 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTAAGAATTC AAGGCCTCTC   841 GAGCCTCTAG AGTTAACATC GAGGGATCAA GCTTATCGAT AATCAACCTC TGGATTACAA   901 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA   961 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC  1021 CTTGTATAAA TCCTGGATTG CTGTCTCTTT ATGAGGAGTG TGGCTCGTGT CAAGGCAACG  1081 TGCGTGTGTG CACCTGTGTT TGCTGACGCA ACCCCCACTG ATGGGGCCAT TGGCCACCAC 1141 CTGATCAGCT TCCTTTCCCG GGG //
Primer: CMV-Forward Sequence file: RDB12910_A4Jfd.seq
>12910_12910_A4Jf_CMV_Forward_1_G03_21.ab1     1 ATTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC    61 AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC   121 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT   181 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC   241 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA   301 GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG   361 CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG   421 TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA   481 AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA   541 GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG   601 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA   661 GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GAAGCTTTAG   721 ACAGATAGAG GAGAGCAACA AAGTAGACCA CCGCCACAGC AAGGGCGCTG ATCTTCAGAC   781 TGAGGAGGAG AATATGAGGG ACAATGGAGG AGTGATTTAT TATAAAATAT AAGTAGTAAA   841 ATGATCATAG GAAGTAGCAC TCCACCAGCA AAAAGAAAAT GGTGCAGAGG AAAAGAGCAT   901 GGATAGAGCT TGTTCTGGAT TCTTGGGACG CAGAAGCCTA TGGCACGTCA TGACTGACGG 961 TAGCTGACAA TATGTCTGTA TGA //
Primer: BGH_rev Sequence file: RDB12910_A4Jfe.seq
>12910_12910_A4Jf_BGH_rev_1_B04_04.ab1     1 CAAATACGGA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT    61 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG   121 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA   181 CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC ATGTGATGAA ATGCTAGGCG   241 GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA TCCATCCCAT GCAGGCTCAC   301 AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT TCTACCTTCT CTTGCTCAAC   361 TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT CTGATCCCTG GCCCTGGTGT   421 GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG ATCCACAGAT CAAGGATATC   481 TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT   541 AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT ACCTGAGGTG TGACTGGAAA   601 ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA TCAGCATTGG TAGCTGCTGT   661 ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA CGGTATCGAT GCGGGGAGGC   721 GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA AGACGCGGAA GAGGCCGCAG   781 AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC CGCTGGATTG AGGGCCGAAG GGACGTAGCA   841 GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG AGCAGCCATG GAAAGGACGT   901 CAGCTTCCCC GACAACACCA CGGAATTGTC AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA   961 GCGGGCAAGC AGCGGCGATG AGTTCCGCCG TGGCAATAGG GAGGGGGAAA GCGAAAGTCC  1021 CGGAAAGGAG CTGACAGGTG GTGGCAATGC CCCAACCAGT GGGGGTTGCG TCAGCAAACA  1081 CAGTGCACCA CCACGCCACG TGCTGAACAC GGGCCACAAC TCCCTCATTA GAGACGCCAA 1141 CAAGAATTTA TACAAGAGAA ATGGAGTCAT ACGGAGCATT AGCCCATGAT AC //
Primer: SV40pro_F Sequence file: RDB12910_A4Jff.seq
>12910_12910_A4Jf_SV40pro_F_1_C04_07.ab1     1 ACCCGTCCTG ACTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA    61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC   121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA   181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG   241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG   301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT   361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC   421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG   481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA   541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT   601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA   661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG   721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA   781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGG AGCTTGCGTA   841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACATTCC CACACACAAT   901 ACGAGCCGGA GCATAAAGTT GTAAAGCCTG GGGGTGCCTA TGGAGTGGAG CTACTCACAT   961 AATGCGTGGC GCTCATGCCC GCTTCAGTCG GGAACTGTCT GTGCAGCTGC ATAATGAATC  1021 CGCCACGCCG GGGGAAGGCG TGGCTATTGG TGCCTTCCGT CCCGCCTCAT GACTCGTGGC 1081 CCTGGCGTTC TGTCGGAGTC GTCTCTGAGG CATATCCGTT ACCCAAATTC TCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl