Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-hSox2-IRES2-Venus

Lentivirus vector plasmid of human SOX2 for generating iPS cells.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12909_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 Sequence file: RDB12909_A4Jfa.seq
>12909_12909_A4Jf_pAxEF_F2_2_B03_06.ab1     1 AAATCCCCAT TTAGGTGTCG GTGACACGCT ACCGGTCTCG AGAATTCTGC AGCGGCCATC    61 ACAAGTTTGT ACAAAAAAGC AGGCTCCGCG GCCGCCCCCT TCACCATGTA CAACATGATG   121 GAGACGGAGC TGAAGCCGCC GGGCCCGCAG CAAACTTCGG GGGGCGGCGG CGGCAACTCC   181 ACCGCGGCGG CGGCCGGCGG CAACCAGAAA AACAGCCCGG ACCGCGTCAA GCGGCCCATG   241 AATGCCTTCA TGGTGTGGTC CCGCGGGCAG CGGCGCAAGA TGGCCCAGGA GAACCCCAAG   301 ATGCACAACT CGGAGATCAG CAAGCGCCTG GGCGCCGAGT GGAAACTTTT GTCGGAGACG   361 GAGAAGCGGC CGTTCATCGA CGAGGCTAAG CGGCTGCGAG CGCTGCACAT GAAGGAGCAC   421 CCGGATTATA AATACCGGCC CCGGCGGAAA ACCAAGACGC TCATGAAGAA GGATAAGTAC   481 ACGCTGCCCG GCGGGCTGCT GGCCCCCGGC GGCAATAGCA TGGCGAGCGG GGTCGGGGTG   541 GGCGCCGGCC TGGGCGCGGG CGTGAACCAG CGCATGGACA GTTACGCGCA CATGAACGGC   601 TGGAGCAACG GCAGCTACAG CATGATGCAG GACCAGCTGG GCTACCCGCA GCACCCGGGC   661 CTCAATGCGC ACGGCGCAGC GCAGATGCAG CCCATGCACC GCTACGACGT GAGCGCCCTG   721 CAGTACAACT CCATGACCAG CTCGCAGACC TACATGAACG GCTCGCCCAC CTACAGCATG   781 TCCTACTCGC AGCAGGGCAC CCCTGGCATG GCTCTTGGCT CCATGGGTTC GGTGGTCAAG   841 TCCGAGGCCA GCTCCAGCCC CCCTGTGGTT ACCTCTTCCT CCCACTCCAG GGCGCCCTGC   901 CAGGCCGGGG ACCTCCGGGA CATGATCAGC ATGTATCTCC CCGGCGCCGA GGTGCCGGAA   961 CCCGCCGCCC CCAGCAGACT TCACATGTCC CAGCACTACC AGAGCGGCCC GGTGCCCGGC  1021 ACGGCCATTA ACGGCACACT GCCCCTCTCA CACATGTGAA AGGGTGGGGC GCGCCGACCC  1081 AGCTTTCTGT ACAAGTTGGT TGATTGCGCG GATCGTCCTC TCCTCCTTCT AACGATACTG 1141 ATCGGAAGA //
Primer: IRES-R Sequence file: RDB12909_A4Jfb.seq
>12909_12909_A4Jf_IRES_R_2_F03_18.ab1     1 GCATCAGCGG CCTTCGGCCA GTAACGTTAG GGGGGGGGGA GGGAGAGGGG CGGATCCGCG    61 GCCATCACCA CTTTGTACAA GAAAGCTGGG TCGGCGCGCC CACCCTTTCA CATGTGTGAG   121 AGGGGCAGTG TGCCGTTAAT GGCCGTGCCG GGCACCGGGC CGCTCTGGTA GTGCTGGGAC   181 ATGTGAAGTC TGCTGGGGGC GGCGGGTTCC GGCACCTCGG CGCCGGGGAG ATACATGCTG   241 ATCATGTCCC GGAGGTCCCC GGCCTGGCAG GGCGCCCTGG AGTGGGAGGA AGAGGTAACC   301 ACAGGGGGGC TGGAGCTGGC CTCGGACTTG ACCACCGAAC CCATGGAGCC AAGAGCCATG   361 CCAGGGGTGC CCTGCTGCGA GTAGGACATG CTGTAGGTGG GCGAGCCGTT CATGTAGGTC   421 TGCGAGCTGG TCATGGAGTT GTACTGCAGG GCGCTCACGT CGTAGCGGTG CATGGGCTGC   481 ATCTGCGCTG CGCCGTGCGC ATTGAGGCCC GGGTGCTGCG GGTAGCCCAG CTGGTCCTGC   541 ATCATGCTGT AGCTGCCGTT GCTCCAGCCG TTCATGTGCG CGTAACTGTC CATGCGCTGG   601 TTCACGCCCG CGCCCAGGCC GGCGCCCACC CCGACCCCGC TCGCCATGCT ATTGCCGCCG   661 GGGGCCAGCA GCCCGCCGGG CAGCGTGTAC TTATCCTTCT TCATGAGCGT CTTGGTTTTC   721 CGCCGGGGCC GGTATTTATA ATCCGGGTGC TCCTTCATGT GCAGCGCTCG CAGCCGCTTA   781 GCCTCGTCGA TGAACGGCCG CTTCTCCGTC TCCGACAAAA GTTTCCACTC GGCGCCCAGG   841 CGCTTGCTGA TCTCCGAGTT GTGCATCTTG GGGTTCTCCT GGGCCATCTT GCGCCGCTGC   901 CCGCGGGACC ACACCATGAA GGCATTCATG GGCCGCTTGA CGCGGTCCGG GCTGTTTTTC   961 TGGTTTGCCG CCGGCCGCCG CCGCGGTGGA GTTGCCCGCC GCCGCCCCCC CGAAGTTTGC  1021 TGCGGGCCCG GGCGGCTTCA GCTTCCGTCT TCCATCATGT GTTACATGAT TGAGGGGGCG  1081 TGCGGAGCCT GCTTTTTGGT ACCAACTGTG AATGATGGCT GGCAGAAATT TCTTCGAGGA 1141 ACTCG //
Primer: IRES-F Sequence file: RDB12909_A4Jfc.seq
>12909_12909_A4Jf_IRES_F_2_C03_09.ab1     1 CATTCCCCCT TTCATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG    61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG   121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG   181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC   241 TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC   301 TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT   361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG   421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG   481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA   541 ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA   601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC   661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC   721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG   781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGAA TTCAAGGCCT   841 CTCGAGCCTC TAGAGTTAAC ATCGAGGGAT CAAGCTTATC GATAATCAAC CTCTGGATTA   901 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG   961 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC  1021 CTCCTTGTAT AAATCCTGGT TTGCTGTCTC TTTATGAGGA GTTTGTGGGC CCGTGTCAGG  1081 CAACGGTGAC GTGGTGTGCA CTTGTGTTGG CCTTGACGCA AACCCCCACT TGGTTGGGGG 1141 CCAATTGGCC AACCAACT //
Primer: CMV-Forward Sequence file: RDB12909_A4Jfd.seq
>12909_12909_A4Jf_CMV_Forward_2_A03_03.ab1     1 CTTAACCTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC   121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA   181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG   241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC   301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG   361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG   421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT   481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC   541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC   601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA   661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT   721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA   781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA   841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGGCAAAGAG AAGAGTGGTG   901 CAGAGAGAAA AAAGAGCAGT GGGGAAATAG GAGCTTTGTT CCCTTGGGTT CTTGGGGAGC   961 AGCAGGAAGC ACTATGGGGC GCAGCGGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA  1021 TTGTCTGGTA TAGTGCAGCC AGCAGACATT TGCTGAAGGC TATTGGAGGG CGCAACAGCC 1081 ATCTGATGCA ACTTCACAGT CTGGGGGCCA TTCAAGTCAA GCTCCAGGCC AAGGAT //
Primer: BGH_rev Sequence file: RDB12909_A4Jfe.seq
>12909_12909_A4Jf_BGH_rev_2_D03_12.ab1     1 GTATATCCGG GGCCCTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT    61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT   121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT   181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA   241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC   301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT   361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG   421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA   481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT   541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG   601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG   661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG   721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC   781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT   841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG   901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC   961 AGCAGCGGGC AAGGCAAGGC GGCGATGAGT TCCCGCCGTG GCAATAGGGA GGGGGAAAAG  1021 CGAAAGTCCC GGGAAAGGAG CTGACAGGTG GTGCAATGCC CCAACCAGTT GGGGGGTTGC  1081 CGTCAGCCAA ACACAGTGCA ACACAACGTC AACGTTGTCT GACCACCGGG CCACAACTTC 1141 TCATTAAGAG GACAGGCCAA CCCAAG //
Primer: SV40pro_F Sequence file: RDB12909_A4Jff.seq
>12909_12909_A4Jf_SV40pro_F_2_E03_15.ab1     1 CTTTCTTTCC GGGGACAATC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT   121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG   181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT   241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG   301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA   361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG   421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT   481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG   541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG   601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC   661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA   721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT   781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG   841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA   901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA   961 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC  1021 ATTAATGAAT CGGGCCAACG CGCGGGGGAG AGGCGGGTTT GCGTATTGGG CGCCTCTTCC  1081 GCTTCCTCGC TCACTGGACC TCGCCTGCCG CTCGGTTCGA TCCGGACTTG CGGCCGAGCC 1141 GTATCAGCTC ACTTTCAAAG GTCGGTTTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl