Resource data sheet
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CSII-EF-hSox2-IRES2-Venus

Lentivirus vector construct of human SOX2 for generating iPS cells.

Catalog number RDB12909
Resource name CSII-EF-hSox2-IRES2-Venus
Clone info. Lentivirus expression vector of human SOX2 for generating iPS cells.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human SOX2 cDNA
Originator Miyoshi, Hiroyuki |
 
Other clones in our bank human SOX2 (NCBI Gene 6657) | human SOX2 (NCBI Gene 6657) |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12909 CSII-EF-hSox2-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12909z.seq from Depositor
Electronic file PDF RDB12909.pdf from Depositor

Featured content

References

original Fujioka, T., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12909_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 RDB12909_A4Jfa.seq
>12909_12909_A4Jf_pAxEF_F2_2_B03_06.ab1
    1 AAATCCCCAT TTAGGTGTCG GTGACACGCT ACCGGTCTCG AGAATTCTGC AGCGGCCATC
   61 ACAAGTTTGT ACAAAAAAGC AGGCTCCGCG GCCGCCCCCT TCACCATGTA CAACATGATG
  121 GAGACGGAGC TGAAGCCGCC GGGCCCGCAG CAAACTTCGG GGGGCGGCGG CGGCAACTCC
  181 ACCGCGGCGG CGGCCGGCGG CAACCAGAAA AACAGCCCGG ACCGCGTCAA GCGGCCCATG
  241 AATGCCTTCA TGGTGTGGTC CCGCGGGCAG CGGCGCAAGA TGGCCCAGGA GAACCCCAAG
  301 ATGCACAACT CGGAGATCAG CAAGCGCCTG GGCGCCGAGT GGAAACTTTT GTCGGAGACG
  361 GAGAAGCGGC CGTTCATCGA CGAGGCTAAG CGGCTGCGAG CGCTGCACAT GAAGGAGCAC
  421 CCGGATTATA AATACCGGCC CCGGCGGAAA ACCAAGACGC TCATGAAGAA GGATAAGTAC
  481 ACGCTGCCCG GCGGGCTGCT GGCCCCCGGC GGCAATAGCA TGGCGAGCGG GGTCGGGGTG
  541 GGCGCCGGCC TGGGCGCGGG CGTGAACCAG CGCATGGACA GTTACGCGCA CATGAACGGC
  601 TGGAGCAACG GCAGCTACAG CATGATGCAG GACCAGCTGG GCTACCCGCA GCACCCGGGC
  661 CTCAATGCGC ACGGCGCAGC GCAGATGCAG CCCATGCACC GCTACGACGT GAGCGCCCTG
  721 CAGTACAACT CCATGACCAG CTCGCAGACC TACATGAACG GCTCGCCCAC CTACAGCATG
  781 TCCTACTCGC AGCAGGGCAC CCCTGGCATG GCTCTTGGCT CCATGGGTTC GGTGGTCAAG
  841 TCCGAGGCCA GCTCCAGCCC CCCTGTGGTT ACCTCTTCCT CCCACTCCAG GGCGCCCTGC
  901 CAGGCCGGGG ACCTCCGGGA CATGATCAGC ATGTATCTCC CCGGCGCCGA GGTGCCGGAA
  961 CCCGCCGCCC CCAGCAGACT TCACATGTCC CAGCACTACC AGAGCGGCCC GGTGCCCGGC
 1021 ACGGCCATTA ACGGCACACT GCCCCTCTCA CACATGTGAA AGGGTGGGGC GCGCCGACCC
 1081 AGCTTTCTGT ACAAGTTGGT TGATTGCGCG GATCGTCCTC TCCTCCTTCT AACGATACTG
1141 ATCGGAAGA
//
Primer: IRES-R RDB12909_A4Jfb.seq
>12909_12909_A4Jf_IRES_R_2_F03_18.ab1
    1 GCATCAGCGG CCTTCGGCCA GTAACGTTAG GGGGGGGGGA GGGAGAGGGG CGGATCCGCG
   61 GCCATCACCA CTTTGTACAA GAAAGCTGGG TCGGCGCGCC CACCCTTTCA CATGTGTGAG
  121 AGGGGCAGTG TGCCGTTAAT GGCCGTGCCG GGCACCGGGC CGCTCTGGTA GTGCTGGGAC
  181 ATGTGAAGTC TGCTGGGGGC GGCGGGTTCC GGCACCTCGG CGCCGGGGAG ATACATGCTG
  241 ATCATGTCCC GGAGGTCCCC GGCCTGGCAG GGCGCCCTGG AGTGGGAGGA AGAGGTAACC
  301 ACAGGGGGGC TGGAGCTGGC CTCGGACTTG ACCACCGAAC CCATGGAGCC AAGAGCCATG
  361 CCAGGGGTGC CCTGCTGCGA GTAGGACATG CTGTAGGTGG GCGAGCCGTT CATGTAGGTC
  421 TGCGAGCTGG TCATGGAGTT GTACTGCAGG GCGCTCACGT CGTAGCGGTG CATGGGCTGC
  481 ATCTGCGCTG CGCCGTGCGC ATTGAGGCCC GGGTGCTGCG GGTAGCCCAG CTGGTCCTGC
  541 ATCATGCTGT AGCTGCCGTT GCTCCAGCCG TTCATGTGCG CGTAACTGTC CATGCGCTGG
  601 TTCACGCCCG CGCCCAGGCC GGCGCCCACC CCGACCCCGC TCGCCATGCT ATTGCCGCCG
  661 GGGGCCAGCA GCCCGCCGGG CAGCGTGTAC TTATCCTTCT TCATGAGCGT CTTGGTTTTC
  721 CGCCGGGGCC GGTATTTATA ATCCGGGTGC TCCTTCATGT GCAGCGCTCG CAGCCGCTTA
  781 GCCTCGTCGA TGAACGGCCG CTTCTCCGTC TCCGACAAAA GTTTCCACTC GGCGCCCAGG
  841 CGCTTGCTGA TCTCCGAGTT GTGCATCTTG GGGTTCTCCT GGGCCATCTT GCGCCGCTGC
  901 CCGCGGGACC ACACCATGAA GGCATTCATG GGCCGCTTGA CGCGGTCCGG GCTGTTTTTC
  961 TGGTTTGCCG CCGGCCGCCG CCGCGGTGGA GTTGCCCGCC GCCGCCCCCC CGAAGTTTGC
 1021 TGCGGGCCCG GGCGGCTTCA GCTTCCGTCT TCCATCATGT GTTACATGAT TGAGGGGGCG
 1081 TGCGGAGCCT GCTTTTTGGT ACCAACTGTG AATGATGGCT GGCAGAAATT TCTTCGAGGA
1141 ACTCG
//
Primer: IRES-F RDB12909_A4Jfc.seq
>12909_12909_A4Jf_IRES_F_2_C03_09.ab1
    1 CATTCCCCCT TTCATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG
  121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
  181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
  241 TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
  301 TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
  361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG
  421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG
  481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA
  541 ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA
  601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC
  661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC
  721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG
  781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGAA TTCAAGGCCT
  841 CTCGAGCCTC TAGAGTTAAC ATCGAGGGAT CAAGCTTATC GATAATCAAC CTCTGGATTA
  901 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG
  961 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC
 1021 CTCCTTGTAT AAATCCTGGT TTGCTGTCTC TTTATGAGGA GTTTGTGGGC CCGTGTCAGG
 1081 CAACGGTGAC GTGGTGTGCA CTTGTGTTGG CCTTGACGCA AACCCCCACT TGGTTGGGGG
1141 CCAATTGGCC AACCAACT
//
Primer: CMV-Forward RDB12909_A4Jfd.seq
>12909_12909_A4Jf_CMV_Forward_2_A03_03.ab1
    1 CTTAACCTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
  781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA
  841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGGCAAAGAG AAGAGTGGTG
  901 CAGAGAGAAA AAAGAGCAGT GGGGAAATAG GAGCTTTGTT CCCTTGGGTT CTTGGGGAGC
  961 AGCAGGAAGC ACTATGGGGC GCAGCGGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA
 1021 TTGTCTGGTA TAGTGCAGCC AGCAGACATT TGCTGAAGGC TATTGGAGGG CGCAACAGCC
1081 ATCTGATGCA ACTTCACAGT CTGGGGGCCA TTCAAGTCAA GCTCCAGGCC AAGGAT
//
Primer: BGH_rev RDB12909_A4Jfe.seq
>12909_12909_A4Jf_BGH_rev_2_D03_12.ab1
    1 GTATATCCGG GGCCCTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
   61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA
  241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC
  301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT
  361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG
  421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA
  481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT
  541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG
  601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG
  661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG
  721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC
  781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT
  841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG
  901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC
  961 AGCAGCGGGC AAGGCAAGGC GGCGATGAGT TCCCGCCGTG GCAATAGGGA GGGGGAAAAG
 1021 CGAAAGTCCC GGGAAAGGAG CTGACAGGTG GTGCAATGCC CCAACCAGTT GGGGGGTTGC
 1081 CGTCAGCCAA ACACAGTGCA ACACAACGTC AACGTTGTCT GACCACCGGG CCACAACTTC
1141 TCATTAAGAG GACAGGCCAA CCCAAG
//
Primer: SV40pro_F RDB12909_A4Jff.seq
>12909_12909_A4Jf_SV40pro_F_2_E03_15.ab1
    1 CTTTCTTTCC GGGGACAATC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
  841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
  901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
  961 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
 1021 ATTAATGAAT CGGGCCAACG CGCGGGGGAG AGGCGGGTTT GCGTATTGGG CGCCTCTTCC
 1081 GCTTCCTCGC TCACTGGACC TCGCCTGCCG CTCGGTTCGA TCCGGACTTG CGGCCGAGCC
1141 GTATCAGCTC ACTTTCAAAG GTCGGTTTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fujioka, T., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836.
user report Hayashi, R., Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. PLoS One 7 (9): e45435 (2012). PMID 23029008.

2018.10.22

GNP_filter3_RDBDEP_html_181012.pl