Please review the QC test results indicated by icon below as well as clone information before placing your order.

pENTR/SV40LT

Plasmid vector of SV40 large T antigen.

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12894_A8Ajp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F2 (Pr0531) Region: rrnBT1 ter, attL1, SV40 LT 5' Sequence file: RDB12894_A8Aja.seq
>RDB12894_A8Aj_3_rrnBT1ter_F2_E03_15_ABI24.ab1     1 CTTTTCCCGG ACTGAGCCTT TCGTTTTATT TGATGCCTGG CAGTTCCCTA CTCTCGCGTT    61 AACGCTAGCA TGGATGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTCTTAAGCT   121 CGGGCCCCAA ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA   181 TGAGCAATGC TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCCG CGGCCGCCCC   241 CTTCACCATG GATAAAGTTT TAAACAGAGA GGAATCTTTG CAGCTAATGG ACCTTCTAGG   301 TCTTGAAAGG AGTGCCTGGG GGAATATTCC TCTGATGAGA AAGGCATATT TAAAAAAATG   361 CAAGGAGTTT CATCCTGATA AAGGAGGAGA TGAAGAAAAA ATGAAGAAAA TGAATACTCT   421 GTACAAGAAA ATGGAAGATG GAGTAAAATA TGCTCATCAA CCTGACTTTG GAGGCTTCTG   481 GGATGCAACT GAGATTCCAA CCTATGGAAC TGATGAATGG GAGCAGTGGT GGAATGCCTT   541 TAATGAGGAA AACCTGTTTT GCTCAGAAGA AATGCCATCT AGTGATGATG AGGCTACTGC   601 TGACTCTCAA CATTCTACTC CTCCAAAAAA GAAGAGAAAG GTAGAAGACC CCAAGGACTT   661 TCCTTCAGAA TTGCTAAGTT TTTTGAGTCA TGCTGTGTTT AGTAATAGAA CTCTTGCTTG   721 CTTTGCTATT TACACCACAA AGGAAAAAGC TGCACTGCTA TACAAGAAAA TTATGGAAAA   781 ATATTCTGTA ACCTTTATAA GTAGGCATAA CAGTTATAAT CATAACATAC TGTTTTTTCT   841 TACTCCACAC AGGCATAGAG TGTCTGCTAT TAATAACTAT GCTCAAAAAT TGTGTACCTT   901 TAGCTTTTTA ATTTGTAAAG GGGTTAATAA GGAATATTTG ATGTATAGTG CCTTGACTAG   961 AGATCCATTT TCTGTTATTG AGGAAAGTTT GCCAGGTGGG TTAAAAGGAG CATGATTTTA  1021 ATCCAGAAAG AAGCAGAGGA AACTAAACAA GTGTCCTGCA AGCTTGTAAC AGAAGTATGC  1081 AATGCAAACA AAATGTGATG ATGTGATGCT AATGCCTTGG GAATGTACTT GCAATTTCAG 1141 GT //
Primer: pDONR_R (Pr0071) Region: T7 pro,attL2, SV40 LT 3' Sequence file: RDB12894_A8Ajb.seq
>RDB12894_A8Aj_3_pDONR_R_F03_18_ABI24.ab1     1 GCCCGACGGG CAGGGAACAA GCTATGACCA TGTAATACGA CTCACTATAG GGGATATCAG    61 CTGGATGGCA AATAATGATT TTATTTTGAC TGATAGTGAC CTGTTCGTTG CAACAAATTG   121 ATAAGCAATG CTTTCTTATA ATGCCAACTT TGTACAAGAA AGCTGGGTCG GCGCGCCCAC   181 CCTTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC   241 TACAAATGTG GTATGGCTGA TTATGATCAT GAACAGACTG TGAGGACTGA GGGGCCTGAA   301 ATGAGCCTTG GGACTGTGAA TCAATGCCTG TTTCATGCCC TGAGTCTTCC ATGTTCTTCT   361 CCCCACCATC TTCATTTTTA TCAGCATTTT CCTGGCTGTC TTCATCATCA TCATCACTGT   421 TTCTTAGCCA ATCTAAAACT CCAATTCCCA TAGCCACATT AAACTTCATT TTTTGATACA   481 CTGACAAACT AAACTCTTTG TCCAATCTCT CTTTCCACTC CACAATTCTG CTCTGAATAC   541 TTTGAGCAAA CTCAGCCACA GGTCTGTACC AAATTAACAT AAGAAGCAAA GCAATGCCAC   601 TTTGAATTAT TCTCTTTTCT AACAAAAACT CACTGCGTTC CAGGCAATGC TTTAAATAAT   661 CTTTGGGCCT AAAATCTATT TGTTTTACAA ATCTGGCCTG CAGTGTTTTA GGCACACTGT   721 ACTCATTCAT GGTGACTATT CCAGGGGGAA ATATTTGAGT TCTTTTATTT AGGTGTTTCT   781 TTTCTAAGTT TACCTTAACA CTGCCATCCA AATAATCCCT TAAATTGTCC AGGTTATTAA   841 TTCCCTGACC TGAAGGCAAA TCTCTGGACT CCCCTCCAGT GCCCTTTACA TCCTCAAAAA   901 CTACTAAAAA CTGGTCAATA GCTACTCCTA GCTCAAAGTT CAGCCTGTCC AAAGGGCAAA   961 TTAACATTTA AAGCTTTCCC CCCACATAAT TCAAGCAAAG CAGCTGCTAA TGTAGTTTTA  1021 CCACTATCAA TTGGTCCTTT AACAGCCCAG TTATCTTTTT TTTTAGAATG TTGTACACCA  1081 TGCATTTTTA AAAAGTCATA CACACTGAAT CCATTTTGGC ACCAACAGTG TAGCAAGCCA 1141 ACTTCCAAGT CCAACTTCCA ATTTCTTCCT AATGT //
Primer: LargeT antigen innerF1 (Pr0006) Region: SV40 LT mid Sequence file: RDB12894_A8Ajc.seq
>RDB12894_A8Aj_3_LargeT antigen innerF1_C01_03_ABI08.ab1     1 ACTGGGTTTT TTGAGGGAAG TTTGCCAGGT GGGTTAAAGG AGCATGATTT TAATCCAGAA    61 GAAGCAGAGG AAACTAAACA AGTGTCCTGG AAGCTTGTAA CAGAGTATGC AATGGAAACA   121 AAATGTGATG ATGTGTTGTT ATTGCTTGGG ATGTACTTGG AATTTCAGTA CAGTTTTGAA   181 ATGTGTTTAA AATGTATTAA AAAAGAACAG CCCAGCCACT ATAAGTACCA TGAAAAGCAT   241 TATGCAAATG CTGCTATATT TGCTGACAGC AAAAACCAAA AAACCATATG CCAACAGGCT   301 GTTGATACTG TTTTAGCTAA AAAGCGGGTT GATAGCCTAC AATTAACTAG AGAACAAATG   361 TTAACAAACA GATTTAATGA TCTTTTGGAT AGGATGGATA TAATGTTTGG TTCTACAGGC   421 TCTGCTGACA TAGAAGAATG GATGGCTGGA GTTGCTTGGC TACACTGTTT GTTGCCCAAA   481 ATGGATTCAG TGGTGTATGA CTTTTTAAAA TGCATGGTGT ACAACATTCC TAAAAAAAGA   541 TACTGGCTGT TTAAAGGACC AATTGATAGT GGTAAAACTA CATTAGCAGC TGCTTTGCTT   601 GAATTATGTG GGGGGAAAGC TTTAAATGTT AATTTGCCCT TGGACAGGCT GAACTTTGAG   661 CTAGGAGTAG CTATTGACCA GTTTTTAGTA GTTTTTGAGG ATGTAAAGGG CACTGGAGGG   721 GAGTCCAGAG ATTTGCCTTC AGGTCAGGGA ATTAATAACC TGGACAATTT AAGGGATTAT   781 TTGGATGGCA GTGTTAAGGT AAACTTAGAA AAGAAACACC TAAATAAAAG AACTCAAATA   841 TTTCCCCCTG GAATAGTCAC CATGAATGAG TACAGTGTGC CTAAAACACT GCAGGCCAGA   901 TTTGTAAAAA CAAATAGATT TTAGGGCCCA AAGGATTATT TAAAAGCATT TGCCTGAAAC   961 GCAGTGAAGT TTTTTGTTTA GAAAAGAAGA ATAAATTCAA GTGGCAATTG CTTTGCTTTC  1021 CTTAATGTTA AATTTGGTAC AGACCTGTGG CTGGAGTTTG CTCAAGTATT CAGAGCAGGA  1081 TTGGTGAAAG CTGGCAAGAG AAGATTTGGA ACGAAGGAGT TTAAGGGTTT TGTGTTCACG 1141 AG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.01.19

GNP_filter3_RDBDEP_html_240118.pl