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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTR/SV40LT

Plasmid vector of SV40 large T antigen.

Catalog number RDB12894
Resource name pENTR/SV40LT
Clone info. Plasmid vector of SV40 large T antigen. Gateway(R) compatible.
Vector backbone pENTR (Plasmid)
Selectable markers Kanamycin
Gene/insert name SV40 Large T antigen cDNA
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12894 pENTR/SV40LT DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12894_A8Ajp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F2
Region: rrnBT1 ter, attL1, SV40 LT 5'
Sequence file: RDB12894_A8Aja.seq check
>RDB12894_A8Aj_3_rrnBT1ter_F2_E03_15_ABI24.ab1
    1 CTTTTCCCGG ACTGAGCCTT TCGTTTTATT TGATGCCTGG CAGTTCCCTA CTCTCGCGTT
   61 AACGCTAGCA TGGATGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTCTTAAGCT
  121 CGGGCCCCAA ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA
  181 TGAGCAATGC TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCCG CGGCCGCCCC
  241 CTTCACCATG GATAAAGTTT TAAACAGAGA GGAATCTTTG CAGCTAATGG ACCTTCTAGG
  301 TCTTGAAAGG AGTGCCTGGG GGAATATTCC TCTGATGAGA AAGGCATATT TAAAAAAATG
  361 CAAGGAGTTT CATCCTGATA AAGGAGGAGA TGAAGAAAAA ATGAAGAAAA TGAATACTCT
  421 GTACAAGAAA ATGGAAGATG GAGTAAAATA TGCTCATCAA CCTGACTTTG GAGGCTTCTG
  481 GGATGCAACT GAGATTCCAA CCTATGGAAC TGATGAATGG GAGCAGTGGT GGAATGCCTT
  541 TAATGAGGAA AACCTGTTTT GCTCAGAAGA AATGCCATCT AGTGATGATG AGGCTACTGC
  601 TGACTCTCAA CATTCTACTC CTCCAAAAAA GAAGAGAAAG GTAGAAGACC CCAAGGACTT
  661 TCCTTCAGAA TTGCTAAGTT TTTTGAGTCA TGCTGTGTTT AGTAATAGAA CTCTTGCTTG
  721 CTTTGCTATT TACACCACAA AGGAAAAAGC TGCACTGCTA TACAAGAAAA TTATGGAAAA
  781 ATATTCTGTA ACCTTTATAA GTAGGCATAA CAGTTATAAT CATAACATAC TGTTTTTTCT
  841 TACTCCACAC AGGCATAGAG TGTCTGCTAT TAATAACTAT GCTCAAAAAT TGTGTACCTT
  901 TAGCTTTTTA ATTTGTAAAG GGGTTAATAA GGAATATTTG ATGTATAGTG CCTTGACTAG
  961 AGATCCATTT TCTGTTATTG AGGAAAGTTT GCCAGGTGGG TTAAAAGGAG CATGATTTTA
 1021 ATCCAGAAAG AAGCAGAGGA AACTAAACAA GTGTCCTGCA AGCTTGTAAC AGAAGTATGC
 1081 AATGCAAACA AAATGTGATG ATGTGATGCT AATGCCTTGG GAATGTACTT GCAATTTCAG
1141 GT
//
Primer: pDONR_R
Region: T7 pro,attL2, SV40 LT 3'
Sequence file: RDB12894_A8Ajb.seq check
>RDB12894_A8Aj_3_pDONR_R_F03_18_ABI24.ab1
    1 GCCCGACGGG CAGGGAACAA GCTATGACCA TGTAATACGA CTCACTATAG GGGATATCAG
   61 CTGGATGGCA AATAATGATT TTATTTTGAC TGATAGTGAC CTGTTCGTTG CAACAAATTG
  121 ATAAGCAATG CTTTCTTATA ATGCCAACTT TGTACAAGAA AGCTGGGTCG GCGCGCCCAC
  181 CCTTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC
  241 TACAAATGTG GTATGGCTGA TTATGATCAT GAACAGACTG TGAGGACTGA GGGGCCTGAA
  301 ATGAGCCTTG GGACTGTGAA TCAATGCCTG TTTCATGCCC TGAGTCTTCC ATGTTCTTCT
  361 CCCCACCATC TTCATTTTTA TCAGCATTTT CCTGGCTGTC TTCATCATCA TCATCACTGT
  421 TTCTTAGCCA ATCTAAAACT CCAATTCCCA TAGCCACATT AAACTTCATT TTTTGATACA
  481 CTGACAAACT AAACTCTTTG TCCAATCTCT CTTTCCACTC CACAATTCTG CTCTGAATAC
  541 TTTGAGCAAA CTCAGCCACA GGTCTGTACC AAATTAACAT AAGAAGCAAA GCAATGCCAC
  601 TTTGAATTAT TCTCTTTTCT AACAAAAACT CACTGCGTTC CAGGCAATGC TTTAAATAAT
  661 CTTTGGGCCT AAAATCTATT TGTTTTACAA ATCTGGCCTG CAGTGTTTTA GGCACACTGT
  721 ACTCATTCAT GGTGACTATT CCAGGGGGAA ATATTTGAGT TCTTTTATTT AGGTGTTTCT
  781 TTTCTAAGTT TACCTTAACA CTGCCATCCA AATAATCCCT TAAATTGTCC AGGTTATTAA
  841 TTCCCTGACC TGAAGGCAAA TCTCTGGACT CCCCTCCAGT GCCCTTTACA TCCTCAAAAA
  901 CTACTAAAAA CTGGTCAATA GCTACTCCTA GCTCAAAGTT CAGCCTGTCC AAAGGGCAAA
  961 TTAACATTTA AAGCTTTCCC CCCACATAAT TCAAGCAAAG CAGCTGCTAA TGTAGTTTTA
 1021 CCACTATCAA TTGGTCCTTT AACAGCCCAG TTATCTTTTT TTTTAGAATG TTGTACACCA
 1081 TGCATTTTTA AAAAGTCATA CACACTGAAT CCATTTTGGC ACCAACAGTG TAGCAAGCCA
1141 ACTTCCAAGT CCAACTTCCA ATTTCTTCCT AATGT
//
Primer: LargeT antigen innerF1
Region: SV40 LT mid
Sequence file: RDB12894_A8Ajc.seq check
>RDB12894_A8Aj_3_LargeT antigen innerF1_C01_03_ABI08.ab1
    1 ACTGGGTTTT TTGAGGGAAG TTTGCCAGGT GGGTTAAAGG AGCATGATTT TAATCCAGAA
   61 GAAGCAGAGG AAACTAAACA AGTGTCCTGG AAGCTTGTAA CAGAGTATGC AATGGAAACA
  121 AAATGTGATG ATGTGTTGTT ATTGCTTGGG ATGTACTTGG AATTTCAGTA CAGTTTTGAA
  181 ATGTGTTTAA AATGTATTAA AAAAGAACAG CCCAGCCACT ATAAGTACCA TGAAAAGCAT
  241 TATGCAAATG CTGCTATATT TGCTGACAGC AAAAACCAAA AAACCATATG CCAACAGGCT
  301 GTTGATACTG TTTTAGCTAA AAAGCGGGTT GATAGCCTAC AATTAACTAG AGAACAAATG
  361 TTAACAAACA GATTTAATGA TCTTTTGGAT AGGATGGATA TAATGTTTGG TTCTACAGGC
  421 TCTGCTGACA TAGAAGAATG GATGGCTGGA GTTGCTTGGC TACACTGTTT GTTGCCCAAA
  481 ATGGATTCAG TGGTGTATGA CTTTTTAAAA TGCATGGTGT ACAACATTCC TAAAAAAAGA
  541 TACTGGCTGT TTAAAGGACC AATTGATAGT GGTAAAACTA CATTAGCAGC TGCTTTGCTT
  601 GAATTATGTG GGGGGAAAGC TTTAAATGTT AATTTGCCCT TGGACAGGCT GAACTTTGAG
  661 CTAGGAGTAG CTATTGACCA GTTTTTAGTA GTTTTTGAGG ATGTAAAGGG CACTGGAGGG
  721 GAGTCCAGAG ATTTGCCTTC AGGTCAGGGA ATTAATAACC TGGACAATTT AAGGGATTAT
  781 TTGGATGGCA GTGTTAAGGT AAACTTAGAA AAGAAACACC TAAATAAAAG AACTCAAATA
  841 TTTCCCCCTG GAATAGTCAC CATGAATGAG TACAGTGTGC CTAAAACACT GCAGGCCAGA
  901 TTTGTAAAAA CAAATAGATT TTAGGGCCCA AAGGATTATT TAAAAGCATT TGCCTGAAAC
  961 GCAGTGAAGT TTTTTGTTTA GAAAAGAAGA ATAAATTCAA GTGGCAATTG CTTTGCTTTC
 1021 CTTAATGTTA AATTTGGTAC AGACCTGTGG CTGGAGTTTG CTCAAGTATT CAGAGCAGGA
 1081 TTGGTGAAAG CTGGCAAGAG AAGATTTGGA ACGAAGGAGT TTAAGGGTTT TGTGTTCACG
1141 AG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.10.02

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