Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-Cre(1A)-IRES2-Venus

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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12889_B1H6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro, attB1,Cre 5' Sequence file: RDB12889_B1H6a.seq
>12889_12889_B1H6_1_EF1a-C_F_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTNN NNANTTTGGN NCTTGGTTNN TTCTCNAGCC TCAGACAGTG    61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAACAC GCTACCGGTC TCGAGAATTC   121 TGCAGCGGCC ATCACAAGTT TGTACAAAAA AGCAGGCTCC ACCATGGGAA CCAATTCAGT   181 CGAGGGGCAG AGCCGATCCT GTACACTTTA CTTAAAACCA TTATCTGAGT GTGAAATGTC   241 CAATTTACTG ACCGTACACC AAAATTTGCC TGCATTACCG GTCGATGCAA CGAGTGATGA   301 GGTTCGCAAG AACCTGATGG ACATGTTCAG GGATCGCCAG GCGTTTTCTG AGCATACCTG   361 GAAAATGCTT CTGTCCGTTT GCCGGTCGTG GGCGGCATGG TGCAAGTTGA ATAACCGGAA   421 ATGGTTTCCC GCAGAACCTG AAGATGTTCG CGATTATCTT CTATATCTTC AGGCGCGCGG   481 TCTGGCAGTA AAAACTATCC AGCAACATTT GGGCCAGCTA AACATGCTTC ATCGTCGGTC   541 CGGGCTGCCA CGACCAAGTG ACAGCAATGC TGTTTCACTG GTTATGCGGC GGATCCGAAA   601 AGAAAACGTT GATGCCGGTG AACGTGCAAA ACAGGCTCTA GCGTTCGAAC GCACTGATTT   661 CGACCAGGTT CGTTCACTCA TGGAAAATAN CGATCGCTGC CAGGATATAC GTAATCTGGC   721 ATTTCTGGGG ATTGCTTANN NCACCCTGTT ACNNATAGCC GAAANTTGCC AGGATCAGGG   781 TNNAAAGATA TCTCACGTAC TGACNGNGGG ANAANGTTNA ATCCNTATTG GCANANNGAA   841 AAACNCTGGN TANCNNNCGC NNGTGNNNNN GAANNNANNT NNNNNNGNGG GNNANNNNAN   901 CNNGGTCNAG NGANGNNNNT NNNNNNNNCT GNNNNNNNTG ANGATNCNNN ANNNANNNNN 961 NNNNN //
Primer: Cre-B_F (Pr1040) Region: Cre 3',attB2,IRES2 Sequence file: RDB12889_B1H6b.seq
>12889_12889_B1H6_1_Cre-B_F_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NNNNCGCGNN NNANGGCCCG CGCTGGNNTT TCNATACCGG    61 AGNTCATGCA AGCTGGTGGC TGGACCAATG TAAATATTGT CATGAACTAT ATCCGTAACC   121 TGGATAGTGA AACAGGGGCA ATGGTGCGCC TGCTGGAAGA TGGCGATTAG CCATTAACGC   181 GAAATACGCG TGCTAGCAAT CTGCAGCGGC CGCACTCGAG ATATCTAGAC CCAGCTTTCT   241 TGTACAAAGT GGTGATGGCC GCGGATCCGC CCCTCTCCCT CCCCCCCCCC TAACGTTACT   301 GGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT ATATGTTATT TTCCACCATA   361 TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC CTGTCTTCTT GACGAGCATT   421 CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC TGTTGAATGT CGTGAAGGAA   481 GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG   541 CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA AGCCACGTGT ATAAGATACA   601 CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT GGATAGTTGT GGAAAGAGTC   661 AAATGGCTCT CCTCAAGCGT ATTCNNNNNN GGGCTGAAGG ATGCCCANAA GGTACCCCAT   721 TGTATGGGAT CTGATCTGGG NNCTCGGTAN NCATGCTTTA CATGTGTTTA NTCGAGGTNN   781 NAAAAACGTC TANGCCCCNC NAAACNNCGG GGACNTGGTT TTCCTTTGAN ANNNNNNNTN 841 NANANNATNG NNNNNACNNA TGGNNNNNNN NNN //
Primer: IRES-F (Pr0036) Region: IRES2 3',Venus 5' Sequence file: RDB12889_B1H6c.seq
>12889_12889_B1H6_1_IRES-F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNTNNNTCNN NGNNNNNNAA CGTCTAGGCC CCCCGAACCA CGGGGNCGTG    61 GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA AGGGCGAGGA   121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA   181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT   241 GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA   301 CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC   361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA   421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA   481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA   541 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA   601 GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC   661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCGC   721 CCTGANCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC   781 CGCCGGGATC ACTCTCGGCA TGGACGANCT GTACAAGTAA GAATTCNAGG CCTCTCGAGC   841 CTCTAGAGTT AACATCGANG GATCAAGCTN ATCGATAATC NACNNCTGGA TTACAAAATT   901 TGTGAAAGAN TGNCNGGTAT TCTTAACNNT GTTGCNNCNT TTTNCGCTAT GNGGATACNC 961 NGCNTTNANG NCNNTNGNAT CATGNNNNNN NNNN //
Primer: PRE_rev (Pr0250) Region: PRE,Venus 3' Sequence file: RDB12889_B1H6d.seq
>12889_12889_B1H6_1_PRE_rev_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GAGCNNNATA GTTAAGAATA CCNGTCAATC TTTCACAAAT    61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGCT   121 CGAGAGGCCT TGAATTCTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG   181 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG   241 GACTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG   301 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG   361 AAGTTGGCCT TGATGCCGTT CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTGGCTGTTG   421 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC   481 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG   541 TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC   601 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG CAGGCCGTAG   661 CCCAGGGTGG TCACGANGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATCAGC   721 TTCAGGGTCA GCTTGCCGTA NGTGGCATCG CCCTCGCCCT CGCCGGANAC GCTGAACTTG   781 TGGCCGTTTA CGTCGCCGNN NNNNTCGANN NNNATGGGCA CCACCCCNGN GAANAGCTNC   841 TCGCCCNTGN NNACCANNGN NNGNGGNCNT ATNANNNTNN NGNTTTTNNA ANNNAAANNN 901 CNNNNNNNNN N //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5' LTR, HIV-1 psi Sequence file: RDB12889_B1H6e.seq
>12889_12889_B1H6_1_CMV-Forward_E03_15_ABI24.ab1     1 NNNNNNNNNN NCNNNTNGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC   121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA   181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG   241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC   301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG   361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG   421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT   481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC   541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC   601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA   661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT   721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCNNA   781 TCTTCAGACC TGGANGANGA NATATGANGG ACAATTGGAG AAGTGAATTA TATAAATATA   841 AAGTAGTAAA AATTGAACCA TTANGAGTAG CACCCACCNA GGNAAAGANA ANANNGGTGC   901 ANANNNNAAA AAAGAGCANN GGGAATANGA NCTTTGTNCC TNNGNNNCTN GGGANCNNCN 961 NNNGNNCTNN NGNNNNNNNN NN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,cPPT_CTS,RRE Sequence file: RDB12889_B1H6f.seq
>12889_12889_B1H6_1_EF1a-N_R_G03_21_ABI24.ab1     1 NNNNNNNNNN NGNNNNNNNN NNNNCGNNGT GCGCTCTGCC NACTGNCGGG CACCGGNNCC    61 TCACGAAGCT TGAATTAATT CAAAGTGAAT TTCTGCTGTC CCTGTAATAA ACCCGAAAAT   121 TTTGAATTTT TGTAATTTGT TTTTGTAATT CTTTAGTTTG TATGTCTGTT GCTATTATGT   181 CTACTATTCT TTCCCCTGCA CTGTACCCCC CAATCCCCCC TTTTCTTTTA AAATTGTGGA   241 TGAATACTGC CATTTGTAGA TCCGTTCACT AATCGAATGG ATCTGTCTCT GTCTCTCTCT   301 CCACCTTCTT CTTCTATTCC TTCGGGCCTG TCGGGTCCCC TCGGGGTTGG GAGGTGGGTC   361 TGAAACGATA ATGGTGAATA TCCCTGCCTA ACTCTATTCA CTATAGAAAG TACAGCAAAA   421 ACTATTCTTA AACCTACCAA GCCTCCTACT ATCATTATGA ATAATTTTAT ATACCACAGC   481 CAATTTGTTA TGTTAAACCA ATTCCACAAA CTTGCCCATT TATCTAATTC CAATAATTCT   541 TGTTCATTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT TAAGCTTGTG   601 TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCGTGT GATTCCAAAT CTGTTCCAGA   661 GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG TTTTCCAGAG   721 CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTANGTATC TTTCCACAGC CAGGNANTCT   781 TGCCTGGAGC TGCNNGATGC CCCAGACTGT GANTTGCAAC AGANGCTGTT GCGCCTNNAT   841 AGCCCTCANC AAANTGTTCT GCTGCTGCAC TATNNNNNAC NATAATTGNC TGGNCTGNAN   901 NGNNNNNNTC ATTGACNCTG CNNCCNTANN NNTNNCNNNC TGCTCCNNAA NAANCCCNAN 961 GGNAANNAAA NCTNCNNANT NCCNNCNGNN NNTTTTTNNN NNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA, 3' LTR Sequence file: RDB12889_B1H6g.seq
>12889_12889_B1H6_1_bGH_rev3_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGGGCNNA CNACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG   121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC   181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC   241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC   301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA   361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT   421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT   481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG   541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CNGTCCCCCC   601 TTTTCTTTTA AAAAGTGGCT AAGATCTACN GCTGCCTTGT NAGTCNTTGN TCNTAAAGGN 661 ACNTGANGTG NGANTGGNAA ANCCNNCTCN NNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, Zeo Sequence file: RDB12889_B1H6h.seq
>12889_12889_B1H6_1_SV40pro_ori_F_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNGCCG CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC   181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC   241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG   301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG   361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG   421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG   481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC   541 TGCGTGCACT TCGTGGCCGA NGAGCANGAC TGACACGTGC TACGAGATTT CGATTCCACC   601 GCCGCCTTCT ATGAAAGGNT GGGCTTCNGA ATCGNTTTCC GGGANGCCNG CTGGNNGANC   661 CTCNNGCGCG GGGNTNTCNN GCTGNANTNN NCNNCCACCC NNNNNGNTNN NTGNANCTNA 721 NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

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