RDB12889 - CSII-EF-Cre(1A)-IRES2-Venus

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CSII-EF-Cre(1A)-IRES2-Venus (#RDB12889)

Depositor\|Developer	Miyoshi, Hiroyuki \|

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12889	CSII-EF-Cre(1A)-IRES2-Venus	Please contact us.	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-Cre(1A)-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12889).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12889_B1H6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro, attB1,Cre 5' Sequence file: RDB12889_B1H6a.seq
>12889_12889_B1H6_1_EF1a-C_F_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNTTNN NNANTTTGGN NCTTGGTTNN TTCTCNAGCC TCAGACAGTG 61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAACAC GCTACCGGTC TCGAGAATTC 121 TGCAGCGGCC ATCACAAGTT TGTACAAAAA AGCAGGCTCC ACCATGGGAA CCAATTCAGT 181 CGAGGGGCAG AGCCGATCCT GTACACTTTA CTTAAAACCA TTATCTGAGT GTGAAATGTC 241 CAATTTACTG ACCGTACACC AAAATTTGCC TGCATTACCG GTCGATGCAA CGAGTGATGA 301 GGTTCGCAAG AACCTGATGG ACATGTTCAG GGATCGCCAG GCGTTTTCTG AGCATACCTG 361 GAAAATGCTT CTGTCCGTTT GCCGGTCGTG GGCGGCATGG TGCAAGTTGA ATAACCGGAA 421 ATGGTTTCCC GCAGAACCTG AAGATGTTCG CGATTATCTT CTATATCTTC AGGCGCGCGG 481 TCTGGCAGTA AAAACTATCC AGCAACATTT GGGCCAGCTA AACATGCTTC ATCGTCGGTC 541 CGGGCTGCCA CGACCAAGTG ACAGCAATGC TGTTTCACTG GTTATGCGGC GGATCCGAAA 601 AGAAAACGTT GATGCCGGTG AACGTGCAAA ACAGGCTCTA GCGTTCGAAC GCACTGATTT 661 CGACCAGGTT CGTTCACTCA TGGAAAATAN CGATCGCTGC CAGGATATAC GTAATCTGGC 721 ATTTCTGGGG ATTGCTTANN NCACCCTGTT ACNNATAGCC GAAANTTGCC AGGATCAGGG 781 TNNAAAGATA TCTCACGTAC TGACNGNGGG ANAANGTTNA ATCCNTATTG GCANANNGAA 841 AAACNCTGGN TANCNNNCGC NNGTGNNNNN GAANNNANNT NNNNNNGNGG GNNANNNNAN 901 CNNGGTCNAG NGANGNNNNT NNNNNNNNCT GNNNNNNNTG ANGATNCNNN ANNNANNNNN 961 NNNNN //
Primer: Cre-B_F (Pr1040) Region: Cre 3',attB2,IRES2 Sequence file: RDB12889_B1H6b.seq
>12889_12889_B1H6_1_Cre-B_F_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NNNNCGCGNN NNANGGCCCG CGCTGGNNTT TCNATACCGG 61 AGNTCATGCA AGCTGGTGGC TGGACCAATG TAAATATTGT CATGAACTAT ATCCGTAACC 121 TGGATAGTGA AACAGGGGCA ATGGTGCGCC TGCTGGAAGA TGGCGATTAG CCATTAACGC 181 GAAATACGCG TGCTAGCAAT CTGCAGCGGC CGCACTCGAG ATATCTAGAC CCAGCTTTCT 241 TGTACAAAGT GGTGATGGCC GCGGATCCGC CCCTCTCCCT CCCCCCCCCC TAACGTTACT 301 GGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT ATATGTTATT TTCCACCATA 361 TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC CTGTCTTCTT GACGAGCATT 421 CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC TGTTGAATGT CGTGAAGGAA 481 GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG 541 CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA AGCCACGTGT ATAAGATACA 601 CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT GGATAGTTGT GGAAAGAGTC 661 AAATGGCTCT CCTCAAGCGT ATTCNNNNNN GGGCTGAAGG ATGCCCANAA GGTACCCCAT 721 TGTATGGGAT CTGATCTGGG NNCTCGGTAN NCATGCTTTA CATGTGTTTA NTCGAGGTNN 781 NAAAAACGTC TANGCCCCNC NAAACNNCGG GGACNTGGTT TTCCTTTGAN ANNNNNNNTN 841 NANANNATNG NNNNNACNNA TGGNNNNNNN NNN //
Primer: IRES-F (Pr0036) Region: IRES2 3',Venus 5' Sequence file: RDB12889_B1H6c.seq
>12889_12889_B1H6_1_IRES-F_C03_09_ABI24.ab1 1 NNNNNNNNNN NNTNNNTCNN NGNNNNNNAA CGTCTAGGCC CCCCGAACCA CGGGGNCGTG 61 GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA AGGGCGAGGA 121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT 241 GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA 301 CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA 421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA 541 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA 601 GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC 661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCGC 721 CCTGANCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC 781 CGCCGGGATC ACTCTCGGCA TGGACGANCT GTACAAGTAA GAATTCNAGG CCTCTCGAGC 841 CTCTAGAGTT AACATCGANG GATCAAGCTN ATCGATAATC NACNNCTGGA TTACAAAATT 901 TGTGAAAGAN TGNCNGGTAT TCTTAACNNT GTTGCNNCNT TTTNCGCTAT GNGGATACNC 961 NGCNTTNANG NCNNTNGNAT CATGNNNNNN NNNN //
Primer: PRE_rev (Pr0250) Region: PRE,Venus 3' Sequence file: RDB12889_B1H6d.seq
>12889_12889_B1H6_1_PRE_rev_D03_12_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GAGCNNNATA GTTAAGAATA CCNGTCAATC TTTCACAAAT 61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGCT 121 CGAGAGGCCT TGAATTCTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG 181 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG 241 GACTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG 301 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG 361 AAGTTGGCCT TGATGCCGTT CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTGGCTGTTG 421 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC 481 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG 541 TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC 601 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG CAGGCCGTAG 661 CCCAGGGTGG TCACGANGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATCAGC 721 TTCAGGGTCA GCTTGCCGTA NGTGGCATCG CCCTCGCCCT CGCCGGANAC GCTGAACTTG 781 TGGCCGTTTA CGTCGCCGNN NNNNTCGANN NNNATGGGCA CCACCCCNGN GAANAGCTNC 841 TCGCCCNTGN NNACCANNGN NNGNGGNCNT ATNANNNTNN NGNTTTTNNA ANNNAAANNN 901 CNNNNNNNNN N //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5' LTR, HIV-1 psi Sequence file: RDB12889_B1H6e.seq
>12889_12889_B1H6_1_CMV-Forward_E03_15_ABI24.ab1 1 NNNNNNNNNN NCNNNTNGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC 121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA 181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG 241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC 301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG 361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG 421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT 481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC 541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC 601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA 661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT 721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCNNA 781 TCTTCAGACC TGGANGANGA NATATGANGG ACAATTGGAG AAGTGAATTA TATAAATATA 841 AAGTAGTAAA AATTGAACCA TTANGAGTAG CACCCACCNA GGNAAAGANA ANANNGGTGC 901 ANANNNNAAA AAAGAGCANN GGGAATANGA NCTTTGTNCC TNNGNNNCTN GGGANCNNCN 961 NNNGNNCTNN NGNNNNNNNN NN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,cPPT_CTS,RRE Sequence file: RDB12889_B1H6f.seq
>12889_12889_B1H6_1_EF1a-N_R_G03_21_ABI24.ab1 1 NNNNNNNNNN NGNNNNNNNN NNNNCGNNGT GCGCTCTGCC NACTGNCGGG CACCGGNNCC 61 TCACGAAGCT TGAATTAATT CAAAGTGAAT TTCTGCTGTC CCTGTAATAA ACCCGAAAAT 121 TTTGAATTTT TGTAATTTGT TTTTGTAATT CTTTAGTTTG TATGTCTGTT GCTATTATGT 181 CTACTATTCT TTCCCCTGCA CTGTACCCCC CAATCCCCCC TTTTCTTTTA AAATTGTGGA 241 TGAATACTGC CATTTGTAGA TCCGTTCACT AATCGAATGG ATCTGTCTCT GTCTCTCTCT 301 CCACCTTCTT CTTCTATTCC TTCGGGCCTG TCGGGTCCCC TCGGGGTTGG GAGGTGGGTC 361 TGAAACGATA ATGGTGAATA TCCCTGCCTA ACTCTATTCA CTATAGAAAG TACAGCAAAA 421 ACTATTCTTA AACCTACCAA GCCTCCTACT ATCATTATGA ATAATTTTAT ATACCACAGC 481 CAATTTGTTA TGTTAAACCA ATTCCACAAA CTTGCCCATT TATCTAATTC CAATAATTCT 541 TGTTCATTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT TAAGCTTGTG 601 TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCGTGT GATTCCAAAT CTGTTCCAGA 661 GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG TTTTCCAGAG 721 CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTANGTATC TTTCCACAGC CAGGNANTCT 781 TGCCTGGAGC TGCNNGATGC CCCAGACTGT GANTTGCAAC AGANGCTGTT GCGCCTNNAT 841 AGCCCTCANC AAANTGTTCT GCTGCTGCAC TATNNNNNAC NATAATTGNC TGGNCTGNAN 901 NGNNNNNNTC ATTGACNCTG CNNCCNTANN NNTNNCNNNC TGCTCCNNAA NAANCCCNAN 961 GGNAANNAAA NCTNCNNANT NCCNNCNGNN NNTTTTTNNN NNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA, 3' LTR Sequence file: RDB12889_B1H6g.seq
>12889_12889_B1H6_1_bGH_rev3_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN GNNGGGCNNA CNACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG 121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC 181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC 241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC 301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA 361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT 421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT 481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG 541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CNGTCCCCCC 601 TTTTCTTTTA AAAAGTGGCT AAGATCTACN GCTGCCTTGT NAGTCNTTGN TCNTAAAGGN 661 ACNTGANGTG NGANTGGNAA ANCCNNCTCN NNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, Zeo Sequence file: RDB12889_B1H6h.seq
>12889_12889_B1H6_1_SV40pro_ori_F_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNGCCG CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT 61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC 181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC 241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG 301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG 361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG 421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG 481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC 541 TGCGTGCACT TCGTGGCCGA NGAGCANGAC TGACACGTGC TACGAGATTT CGATTCCACC 601 GCCGCCTTCT ATGAAAGGNT GGGCTTCNGA ATCGNTTTCC GGGANGCCNG CTGGNNGANC 661 CTCNNGCGCG GGGNTNTCNN GCTGNANTNN NCNNCCACCC NNNNNGNTNN NTGNANCTNA 721 NNNNNNNNNN //