Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSIV-RfA-CG

Sequence (full)	RDB12884zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12884_B3Gep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R (Pr0012) Region: CmR,attR1,luc UV50 pro,cPPT_CTS Sequence file: RDB12884_B3Gea.seq
>12884_12884_B3Ge_1_Cm_M_R_F02_06_ABI08.ab1     1 NNNNNNNNNN NNNNNCNNNT ATNNCNNGCT CNCCNNCTTN NNNTGCCATA CGGAATTCCG    61 GATGNAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA   121 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA   181 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA   241 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC   301 GACGGATCCT AACTCAAAAT CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT   361 GGGGTGCCTA ATGCGGCCGC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG   421 TCTGTTTTTT ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC   481 GTTCAGCTTT TTTGTACAAA CTTGTGATAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT   541 AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG   601 TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT   661 TAAAATTGTG GNNNNANACT GNCNTTTGTA GATNCGTTNA CNNANTCNNN NNNNNNTGTC 721 NCTNNCTCTC TCNNNNNNNT NNNNNNNNNN //
Primer: ccdb-M_F (Pr0951) Region: ccdB,attR2,CMV en.2 Sequence file: RDB12884_B3Geb.seq
>12884_12884_B3Ge_1_ccdb-M_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNGNNNNA NTGNNTGNNC TNNGCNNCCG CGNAAATGAC ATCAAAAACG    61 CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG   121 CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA   181 TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC   241 TTGTACAAAG TGGTGATAAT TCGATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG   301 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC   361 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG   421 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA   481 TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC   541 CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT   601 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA   661 GCGGTTTGAC TCACGGGGNN TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT   721 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA   781 AATGNNCGGT ANGCGTGTAC GGNGGGNNGN CTATATAAGC AGANCTNGTT TANTGAANCG   841 TCANANCCGC TANCGCTACN NNNCGCCACN NNGGNGANCA ANGGCNANNN NCNNNTCNNN 901 NGGGNNNNNN NCNNTCCNNN NNNANNNNGN NNNGNNNNNN NNANNNNNNN NNN //
Primer: PRE_rev (Pr0250) Region: PRE,EGFP 3' Sequence file: RDB12884_B3Gec.seq
>12884_12884_B3Ge_1_PRE_rev_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT    61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGCT   121 CGAGATCCTA CTAGTAGGAT CTGAGTCCGG ACTTGTACAG CTCGTCCATG CCGAGAGTGA   181 TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT   241 TGCTCAGGGC GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC   301 CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT   361 GGCGGATCTT GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT   421 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT   481 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC   541 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGANGGTGCG CTCCTGGACG TAGCCTTCGG   601 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT   661 GCACGCCGNN NNTCNNNNNG GTCACNANGG TGGGNCNGGG NNCNGNNNGN NTNNNNNNNN 721 GNNNNNNNNN N //
Primer: HIV-B_R (Pr0673) Region: 5' LTR,CMV pro.1,CMV en.1 Sequence file: RDB12884_B3Ged.seq
>12884_12884_B3Ge_1_HIV-B_R_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAA NNTTTTGNNG TANTCNCCAG TCGCCGCCCC TCGCCTCTTG    61 CCGTGCNNNN NNNNNNNNGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGN TNNNNTTTCG   121 CTTTCAAGTC CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT   181 GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA   241 AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC   301 TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT   361 CCCACCGTAC ACGCCTACCG CCCATTTGCG TCAATGGGGC GGAGTTGTTA CGACATTTTG   421 GAAAGTCCCG TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG GGGTGGAGAC   481 TTGGAAATCC CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC CAAAACCGCA   541 TCACCATGGT AATAGCGATG ACTAATACGT AGATGTACTG CCAAGTAGGA AAGTCCCATA   601 AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG TCAATAGGGG   661 GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCNAGTG GGCAGTTTAC CGTAAATACT   721 CCACCCATTG ACGTCAATGG AAAGTCCCTA TTGNCGTTAC TATGGGAACA TACGTCATTA   781 TTGACGTCNA TGNNNGGGGG TCGTTGGGCG GNCAGCCAGG CGGGCCATTT ANCGTAAGTT   841 ATGTAACGCN GNANNTNNNT ATATNGNCTA TNANCTAATG ANCCCNNNAT TGANTNCTAT   901 TNANNACTAN NCNATNANCN ANGNNNNNNN NNATATCTGG NCNGTNNNTC NNNNANNNNN 961 NNNAANNNNN NNANNNNNNN NNN //
Primer: EGFP-N_R2 (Pr0905) Region: EGFP 5',CMV pro.2,CMV en.2 Sequence file: RDB12884_B3Gee.seq
>12884_12884_B3Ge_1_EGFP-N_R2_G02_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGCNNNN CNNNNCNNNC CGNANGTCAG GGNGGTCACG    61 AGGGTGGGCC AGGGNACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG   121 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG   181 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC   241 ATGGTGGCGA CCGGTAGCGC TAGCGGATCT GACGGTTCAC TAAACCAGCT CTGCTTATAT   301 AGACCTCCCA CCGTACACGC CTACCGCCCA TTTGCGTCAA TGGGGCGGAG TTGTTACGAC   361 ATTTTGGAAA GTCCCGTTGA TTTTGGTGCC AAAACAAACT CCCATTGACG TCAATGGGGT   421 GGAGACTTGG AAATCCCCGT GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCAAA   481 ACCGCATCAC CATGGTAATA GCGATGACTA ATACGTAGAT GTACTGCCAA GTAGGAAAGT   541 CCCATAAGGT CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA TTGACGTCAA   601 TAGGGGGCGT ACTTGGCATA TGATACACTT GNTGTACTGC CNAGTGGGCA GTTTACCGTA   661 AATACTCNAC CCATTGACGT CNATGNAAAG TCCCNTATTN NNNNTACTAN NGGNNNCNTA 721 CNNNNNTNNN TNNNNNNNNN N //
Primer: EGFP-M_R (Pr0743) Region: EGFP 5',CMV pro.2 Sequence file: RDB12884_B3Gef.seq
>12884_12884_B3Ge_1_EGFP-M_R_H02_08_ABI08.ab1     1 NNNNNNNNNN NNNNGNGGNG GNNCTTGNNN TTCNCCTTGA TGCCGTTCTT CTGCTTGTCG    61 GCCATGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG   121 CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG   181 AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC   241 TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG   301 TAGCGGCTGA AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG   361 GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC   421 TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG   481 ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTGGC GACCGGTAGC   541 GCTAGCGGAT CTGACGGTTC ACTAAACCAG CTCTGCTTAT ATAGACCTCC CACCGTACAC   601 GCCTACCGCC CATTTGCGTC AATGGGGCGG ANTTGTTACG ACATTTTGGA AAGTNCCGTT 661 GNNTTTGGTG NCNAAACAAA CTCCCATTGN CGTCNANNGG GGNNNNNNNN NN //
Primer: bGH_rev3 (Pr0885) Region: 3' LTR,loxP,PRE Sequence file: RDB12884_B3Geg.seq
>12884_12884_B3Ge_1_bGH_rev3_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGGCNNNNA ACAGNNGGCT GGCAACTAGA AGGCACAGTC    61 GAGGCTGATC NNNNNNTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG   121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC   181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA   241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGAATAACTT CGTATAATGT   301 ATGCTATACG AAGTTATGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC   361 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA   421 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA   481 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT   541 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG   601 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA   661 TTGAGGGCCG AANGGACGTA NCAGAANGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG   721 GCGANCAGCC ATGGAAAGGA CGTCAGCTTC CCCGACAACA CCACGGAATT GTCAGTGCCC   781 AACAGCCGAN CCCCTGTCCA GCANCGGGCA AGGCANGCGG CGATGANTTC CGCNNNNNCN   841 ATANGGANGG GGGAAAGCGA ANNTCCNNGA AANGANCTGA NANGNNGNGG CAANGNCCNN   901 NCCAGNGGGG GGTTGCGNCN NCNAACACNG NGCNNNNNAN NNCNNNNNTN NNCNNNNNNN 961 NNGGNNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: Zeo,SV40 pA Sequence file: RDB12884_B3Geh.seq
>12884_12884_B3Ge_1_SV40pro_ori_F_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNGCCNC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT    61 TTTGGAGNNN NNNNNNNTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT NNNNNNATCT   121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA   181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG   241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG   301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG   361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT   421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA   481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT   541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG   601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC   661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT   721 ATAATGNTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC   781 TGCATTCTAN TTGTGGTTTG TCCNAACTCA TCAATGTATC TTATCATGTC TGTATACCGT   841 CNANCTCTAN CTAGANCTTG GCGTAATCAT GGNCATANCN GNTNCCTGNG NGAAATTGTT   901 NTCCNCTCNC NATTCCNCNN NNNATACNAN CCNGANNNAT AAANNGNAAA GNCNNGGGNN 961 GNCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.01

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