Resource data sheet
Prev. |  Lentiviral vector plasmid > 
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSIV-RfA

Backbone vector plasmid of lentivirus construct.

Catalog number RDB12882
Resource name CSIV-RfA
Clone info. Backbone vector plasmid of lentivirus construct. Please note that this construct contains loxP.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-RfA (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12882 CSIV-RfA DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence compiled by us RDB12882zzt01.seq

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12882_B0Jkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R
Region: CmR, lacUV5 pro, attR1, cPPT_CTS
Sequence file: RDB12882_B0Jka.seq check
>12882_12882_B0Jk_2_Cm-M_R_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNATNNCNNG CTCNCCGTCT TTNNTTGCCN TACGGAATTC
   61 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT
  121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT
  181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC
  241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT
  301 CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC
  361 TGGGGTGCCT AATGCGGCCG CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA
  421 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT
  481 CGTTCAGCTT TTTTGTACAA ACTTGTGATA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA
  541 TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT
  601 GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCCNATCC CCCCTTTTCN
  661 TTNAAAANTG NGGNNNAANA CTGNCATTTG TANANCCNNN CNCTAATCNN ANNNNNNNNG
721 NNNNNGNNNN NNNNNN
//
Primer: ccdB-C_F
Region: ccdB, attR2, PRE
Sequence file: RDB12882_B0Jkb.seq check
>12882_12882_B0Jk_ccdB-C_F_E08_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNNNNNNN NTGGCGCATG ATGACCACCG ATATGGCCAG
   61 TGTGCCGGTC TCCGTTATCG GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT
  121 CAAAAACGCC ATTAACCTGA TGTTCTGGGG AATATAAATG TCAGGCTCCC TTATACACAG
  181 CCAGTCTGCA GGTCGACCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT
  241 GTTTTTTATG CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT
  301 CAGCTTTCTT GTACAAAGTG GTGATGCTAC CGGTCTCGAG CCTCTAGAGT TAACATCGAG
  361 GGATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA
  421 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC
  481 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT
  541 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG
  601 CTGACNCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCNNCTCCTT TCCGGGACTT
  661 TCGCTTTCCC CNTCCCNATT GNNNCNNNNN NNNCATCNNN NNNNGNCNTN NNCCNCTGCT
721 NNNNNNNNNN
//
Primer: CMV_Forward
Region: CMV pro, 5' LTR, HIV-1 psi
Sequence file: RDB12882_B0Jkc.seq check
>12882_12882_B0Jk_CMV-Forward_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNN NNNTGGGCGG TAGGCGTGNN NNGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGNCAGCTA CAACCATCCC TTCNGACNGG ATCNGAAGAN CTTANATCNT TATATAATAC
661 NGTANCNACC CTCTANNGNG NGNNTCNNNG ATANNNNNAA ANNNNNNNNN N
//
Primer: RRE_F1
Region: RRE, gp41 peptide
Sequence file: RDB12882_B0Jkd.seq check
>12882_12882_B0Jk_RRE_F1_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANNNTTTGCT GANGGCTATT GAGGCGCAAC AGCATCTGTT
   61 GCAACTCACA GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA ATCCTGGCTG TGGAAAGATA
  121 CCTAAAGGAT CAACAGCTCC TGGGGATTTG GGGTTGCTCT GGAAAACTCA TTTGCACCAC
  181 TGCTGTGCCT TGGAATGCTA GTTGGAGTAA TAAATCTCTG GAACAGATTT GGAATCACAC
  241 GACCTGGATG GAGTGGGACA GAGAAATTAA CAATTACACA AGCTTAATAC ACTCCTTAAT
  301 TGAAGAATCG CAAAACCAGC AAGAAAAGAA TGAACAAGAA TTATTGGAAT TAGATAAATG
  361 GGCAAGTTTG TGGAATTGGT TTAACATAAC AAATTGGCTG TGGTATATAA AATTATTCAT
  421 AATGATAGTA GGAGGCTTGG TAGGTTTAAG AATAGTTTTT GCTGTACTTT CTATAGTGAA
  481 TAGAGTTAGG CAGGGATATT CACCATTATC GTTTCAGACC CACCTCCCAA CCCCGAGGGG
  541 ACCCGACAGG CCCGAANGAA TAGAAGAAGA ANGTGGAGAG AGAGACAGAN ACAGATCCNT
  601 TCGATTAGTG AACGGNTCTA CNAATGGCAG TATTCNTCCN CAATNNNAAA AGAAAAGGGG
  661 GGNNTGGGGG GGNACAGNGN NNNNNNGNAN AGTANANNTN NNNNCNNNNG NNNNNNNANN
721 NNNNNNNTNN NNAANNNANN NNNNNNNN
//
Primer: bGH_rev3
Region: bGHpA, 3' LTR, loxP, PRE
Sequence file: RDB12882_B0Jke.seq check
>12882_12882_B0Jk_bGH_rev3_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNGGGCAAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG
  121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
  181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
  241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGAATAAC TTCGTATAAT
  301 GTATGCTATA CGAAGTTATG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  361 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  421 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  481 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
  541 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGANGGCGAA
  601 NGCGAAGANG CGGAANANGN CGCAGANCCG GCAGCANGNC GCGGGAANGA ANGNNCGCTG
661 GNNTGAGGGN CNAANNGNAC GNANNNNNNN NNGTCCNNCG NNNANTNNNN NNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, EM7 pro, Zeo
Sequence file: RDB12882_B0Jkf.seq check
>12882_12882_B0Jk_SV40pro_ori_F_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNGNCGCCTC TGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
  121 CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG
  181 TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG
  241 TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG
  301 ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC
  361 AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG
  421 CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG
  481 AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG
  541 TGCACTTCGT GGCCGANGAN CANGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG
  601 CCTTCTATGA AAGNNTGGGC TTCNGANTCG TTTTCCGGGA NGCCNGCTGG ATGANNCTCC
661 NGCGCGGGGN NCTCNTGCTG NANTNCNTCG CCNNCCCNNN NNNNNTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.12.24

GNP_filter3_RDBDEP_html_201222.pl