RDB12878 - CSIV-TRE-RfA-UbC-KT

Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by UbC promoter.

Distribution information

Sequence (full)	RDB12878zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB12878.pdf

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内)

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的提供条件：利用者は、hKO1蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。宮脇敦史博士の書面による提供承諾が必要です。

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QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12878	CSIV-TRE-RfA-UbC-KT	DNA solution

Featured content	Lentivirus Vector (English text)
Featured content	Lentivirus Vector (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

original	Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.
reference	Jeong, H.C., Timely Degradation of Wip1 Phosphatase by APC/C Activator Protein Cdh1 is Necessary for Normal Mitotic Progression. J. Cell. Biochem. 116 (8): 1602-1612 (2015). PMID 25649870.
reference	Murata, K., Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis. Biochem. Biophys. Res. Commun. 458 (3): 470-475 (2015). PMID 25660450.
reference	Goshima, T., Mammal-specific H2A variant, H2ABbd, is involved in apoptotic induction via activation of NF-κB signaling pathway. J. Biol. Chem. 289 (17): 11656-11666 (2014). PMID 24584930.
user_report	Kubota, H., RUNX inhibitor suppresses graft-versus-host disease through targeting RUNX-NFATC2 axis. eJHaem. 2 (3): 449-458 (2021).
user_report	Noura, M., TXNIP induces growth arrest and enhances ABT263-induced apoptosis in mixed-lineage leukemia-rearranged acute myeloid leukemia cells. FEBS Open Bio. 10 (8): 1532–1541 (2020). PMID 32511893.
user_report	Noura, M., Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells. Sci. Rep. 10 (1): 20245 (2020). PMID 33219287.
user_report	Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.
user_report	Sato, K., FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5'-DNA end. Nucleic Acids Res. 44 (22): 10758-10771 (2016). PMID 27694619.

Primer: UbC-N_R (Pr0262) Region: UbC pro, attR2, ccdB Sequence file: RDB12878_A4Klh.seq
>12878_12878_B1HpF_UbC-N_R_B02_05_ABI24.ab1 1 NNNNNNNNCN NNNNNNNNCG TCCGGGCGGA NGNNCAGGAC GCTCGCTGCG CCCTTCGTCT 61 GACGTGGCAG CGCTCGCCGT GNGGAGGGGG GCGCCCGCGG GGGGCGCCAA AACCCGGCGC 121 GGAGGCCAGA TCTCCCGATC TCGAGACCGG TAGCATCACC ACTTTGTACA AGAAAGCTGA 181 ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA 241 GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA TGGTCGACCT GCAGACTGGC 301 TGTGTATAAG GGAGCCTGAC ATTTATATTC CCCAGAACAT CAGGTTAATG GCGTTTTTGA 361 TGTCATTTTC GCGGTGGCTG AGATCAGCCA CTTCTTCCCC GATAACGGAG ACCGGCACAC 421 TGGCCATATC GGTGGTCATC ATGCGCCAGC TTTCATCCCC GATATGCACC ACCGGGTAAA 481 GTTCACGGGA GACTTTATCT GACAGCAGAC GTGCACTGGC CAGGGGGATC ACCATCCGTC 541 GCCCGGGCGT GTCAATAATA TCACTCTGTA CATCCACAAA CAGACGATAA CGGCTCTCTC 601 TTTTATAGGT GTAAACCTTA AACTGCATTT CACCAGCCCC TGTTCTCGTC AGCAAAAGAN 661 CCGNTCATTT CNATNAAACC NGGNGANNNC ANCNNTCCCN TNNNNNNTTN NNNNNTNNNN 721 NNNNNN //
Primer: UbCpro_F (Pr0158) Region: hKO1 5' Sequence file: RDB12878_A4Kli.seq
>12878_12878_A4Kl_2_UbCpro_F_A11_02.ab1 1 NNNNNNNNNN NTNNNGNNNN NGNNNNNNNN NTGTCCGCTA AATTCNGGCC GTTTTTGGCT 61 TTTTTGTTAG ACCGGNNCGT GTTTAGCGCT ACCGGTCCAC CATGGTGAGC GTGATCAAGC 121 CCGAGATGAA GATGAAGTAC TTCATGGACG GCAGCGTGAA CGGCCACGAG TTCACCGTGG 181 AGGGCGAGGG CACCGGCAAG CCCTACGAGG GCCACCAGGA GATGACCCTG AGGGTGACAA 241 TGGCCAAGGG CGGCCCCATG CCCTTCAGCT TCGACCTGGT GAGCCACACC TTCTGCTACG 301 GCCACAGGCC CTTCACCAAG TACCCCGAGG AGATCCCCGA CTACTTCAAG CAGGCCTTCC 361 CCGAGGGCCT GAGCTGGGAG AGGAGCCTCC AGTTCGAGGA CGGCGGCTTC GCCGCCGTGA 421 GCGCCCACAT CAGCCTGAGG GGCAACTGCT TCGAGCACAA GAGCAAGTTC GTGGGCGTGA 481 ACTTCCCCGC CGACGGCCCC GTGATGCAGA ACCAGAGCAG CGACTGGGAG CCCAGCACCG 541 AGAAGATCAC CACCTGCGAC GGCGTGCTGA AGGGCGACGT GACCATGTAC CTGAAGCTGG 601 CCGGCGGCGG CAACCACAAG TGCCAGTTCA AGACCACCTA CAAGGCCGCC AAGAAGATCC 661 TGAAGATGCC CCAGAGCCAC TTCATCGGCC ACAGGCTGGT GAGGAAGANC GAGGGCAACA 721 TCACCGAGCT GGTGGAGGAN GCCGTGGCCC ACTGCGAGGG CAGANGAAGT CTTCTAACAT 781 GCGGTGACGT GGANGANAAT CNCGGNCCTA TGTCTAGACT GGACAAGANC AAAGTCATAA 841 ACGGCGCTCT GGAATTACTC NATGNANTCN GTATCGAANG NCTNACGANN NGGAANCTCN 901 NNNNAAAGNT GGGNNTTGAN CAGCCTANCN NNNNNNNNN //
Primer: hKO_F (Pr0157) Region: T2A, rtTA-Advanced Sequence file: RDB12878_A4Klj.seq
>12878_12878_A4Kl_2_hKO_F_B11_05.ab1 1 NNNNNNNNNN NNNGCNNGNG NNGANNNCGN NGGCAACATC ACCNANCTGG TGGAGGANGC 61 CGTGGCCCAC TGNNNNGNNA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC 121 CGGCCCTATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC GGCGCTCTGG AATTACTCAA 181 TGGAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA 241 GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA 301 GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT 361 TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA 421 AGTGCATCTC GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC 481 GTTCCTGTGT CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG 541 CCACTTTACA CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG 601 AGAGACACCT ACCACCGATT CTATGCCCCC ACTTCTGAGA CAAGCAATTG AGCTGTTCGA 661 CCGGCAGGGA GCCGAACCTG CCTTCCTTTT CGGCCTGNAA CTAATCATAT GTGNCCTGNA 721 GAAACAGCTA AAGTGCGAAA GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTANA 781 CATGCTCCCN GCCGANGCCC TTGACGACTT TGANCTTGAT ATGCTGCCTG CTGANGCTCT 841 TGACNANTTT GANCNNGACA TGCTCCCNNG GNAACTAANN NANNNATCCT CTANNNNTNA 901 CNTCNNNGGN NCNNNCNNNN CGATANTCNN NNNCNNNNNT NNNAAATTNN NNNANNNNNN 961 NNNN //
Primer: tTA-Ad R (Pr0542) Region: T2A, hKO1 3' Sequence file: RDB12878_A4Klk.seq
>12878_12878_B1HpF_tTA-Ad R_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNN TTTGCTCTTG TCNNGTCTAG ACATAGGGCC GGGNTTCTCC 61 TCCACGTCAC CGCATGTTAG AAGACTTCCT CTGCCCTCGC AGTGGGCCAC GGCGTCCTCC 121 ACCAGCTCGG TGATGTTGCC CTCGGTCTTC CTCACCAGCC TGTGGCCGAT GAAGTGGCTC 181 TGGGGCATCT TCAGGATCTT CTTGGCGGCC TTGTAGGTGG TCTTGAACTG GCACTTGTGG 241 TTGCCGCCGC CGGCCAGCTT CAGGTACATG GTCACGTCGC CCTTCAGCAC GCCGTCGCAG 301 GTGGTGATCT TCTCGGTGCT GGGCTCCCAG TCGCTGCTCT GGTTCTGCAT CACGGGGCCG 361 TCGGCGGGGA AGTTCACGCC CACGAACTTG CTCTTGTGCT CGAAGCAGTT GCCCCTCAGG 421 CTGATGTGGG CGCTCACGGC GGCGAAGCCG CCGTCCTCGA ACTGGAGGCT CCTCTCCCAG 481 CTCAGGCCCT CGGGGAAGGC CTGCTTGAAG TAGTCGGGGA TCTCCTCGGG GTACTTGGTG 541 AAGGGCCTGT GGCCGTAGCA GAAGGTGTGG CTCACCAGGT CGAAGCTGAA GGGCATGGGG 601 CCGCCCTTGG CCATTGTCAC CCTCANGGTC ATCTCCTGGT GGCCCTCGTA NGGCTTGCCG 661 GTGCCCTCGC CCTNNACGGN GAACTCGTGG NNNNTCNCGC TNNNGTCNNN NANNNNNNNN 721 NN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5'LTR, HIV-1 psi Sequence file: RDB12878_A4Kll.seq
>12878_12878_B1HpF_CMV-Forward_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNCNNTNNN NGCNNTGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA 301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG 361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT 421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG 481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG 541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA 601 CTGGGACAGC TACAACCATC CCTTCNGACA GGNNCAGAAG ANNTTAGNTC ATTATATAAT 661 ACNGTAGCAA CCCTCTANTG TGTGCATCNA NGGATAGANA NAAAANNANN NNNANNNNNN 721 NNTNNNNNNN NNANNNNNNG NNNNNNNNNN //
Primer: Cm_N_rev (Pr0014) Region: attR1, tight TRE pro, cPPT_CTS Sequence file: RDB12878_A4Klm.seq
>12878_12878_A4Kl_2_Cm_N_rev_C11_08.ab1 1 NNNNNNNNNN NNNNTNTTNN NGANGCNNTT GGGNNNTATC AACNGNNNNA TATCCNGTGA 61 TTTTTTTCTC CNTTTNANNT TCCTTAGCTC CTGAAAATCT CGACGGATCC TAACTCAAAA 121 TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGCGGCCG 181 CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT 241 CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TTTTGTACAA 301 ACTTGTGATG CTAGCGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA TATAGGCCTC 361 CCACCGTACA CGCCTACCTC GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA 421 TACGTTCTCT ATCACTGATA GGGATAAACT CGACATACGT TCTCTATCAC TGATAGGGAG 481 TAAACTCGAC ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC GTTCTCTATC 541 ACTGATAGGG AGTAAACTCG ACATCGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA 601 CGTTCTCTAT CACTGATAGG GAGTAAACTC GATTGAAGAC GAAAGGGCCG AATTCAAAGT 661 GAATTTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA TTTTTGTAAT TTGTTTTTGT 721 AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA TTCTTTCCCC TGCACTGTAC 781 CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGANTA CTGCCNTTTG TAGATCCGTT 841 CACTAATCNA ATGGATCTGT CTCTGTCTCT CTCTCCACCT TCTTCTTCNA TTCCTTCNGN 901 NNTGTCNGGN CCCNNCNGGG TTGGNANNNG GNNCTGAAAC GATANNNNNG AATATNCNNN 961 NCNNNNTCTA TTCNCTANNN AAAGNANNNN NAAANNNNNN NNNN //
Primer: Cm_C_F (Pr0476) Region: ccdB Sequence file: RDB12878_A4Kln.seq
>12878_12878_A4Kl_2_Cm_C_F_D11_11.ab1 1 NNNNNNNNNN NNNNNGNNNN NNNNNNNNNN GANAANNGTA TGCNNNNTTG CGCGCTGATT 61 TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTATGC 121 TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA 181 TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG 241 CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT 301 GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGGCTGGT GAAATGCAGT TTAAGGTTTA 361 CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA 421 CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT 481 CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC 541 CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG 601 CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC 661 CCTTATACAC AGCCAGTCTG CAGGTCGACC NTAGTGACTG GATATGTTGT GTTTTACAGT 721 ATTATGTAGT CTGTTTTTTA TGCNAAATCT AATTTAATAT ATTGATATTT ATATCATTTT 781 ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTGATGCT ACCGGTCTCG ANATCGGGAG 841 ATCTGGCCTC CGCGNCNNGT TTTGGCGCCC CCCGNGGGCG CCCCCNNNCT CACGGCGAGC 901 GCTGCCNCNT CAGANGAANN NNNGCANNNA GCGNNCNGAT NNNTNNNNCN NGNNGNNNNN 961 NANANCNNNC NNCNNNTNNN NANNNNNNNG NNNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA, 3'LTR, loxP, PRE Sequence file: RDB12878_A4Klo.seq
>12878_12878_B1HpF_bGH_rev3_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNGNNNNNG GNNNNNNACA GATGGCTGGC AACTAGAAGG CACAGTCGAG 61 GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT 121 GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA 181 AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC 241 TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA ATAACTTCGT ATAATGTATG 301 CTATACGAAG TTATGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC 361 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT 421 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA 481 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA 541 CGGTATCGAT GCGGGGAGGC GGCCCAAAGG GAGNTCCGAC TCGTCTGANG GCGAANGCGA 601 AGANGCGGAA GANGCCGCAG ANCCGGCAGC AGGCCGCGGG AANGAANGNC CGCTGGANTG 661 ANGGCCGAAN GGNNGTANCA NNNNNNGTCC CGCGCANAAN CCNNGNGGCN NNNCANGNNA 721 NNNNNNNNNG NNANNNNNNN NNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, Zeo Sequence file: RDB12878_A4Klp.seq
>12878_12878_B1HpF_SV40pro_ori_F_E02_14_ABI24.ab1 1 NNNNNNNNNN NGNCGCCTCN GCCTCTGNNC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG 61 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC 121 AGCACGTGTT GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT 181 GAGGAACTAA ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT 241 CGCCGGAGCG GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA 301 CGACTTCGCC GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA 361 GGTGGTGCCG GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC 421 CGAGTGGTCG GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA 481 GATCGGCGAG CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT 541 GCACTTCGTG GCCGAGGAGC AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC 601 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 661 GCGCGGGGNN CTCNTGCTGG ANTTCTTCGC CCACCCCNAC TNNNTNNNNG NAGCTTNNNN 721 NGNNTNNCNA NNNAANNNNA NNNNNNNNNN //