Resource data sheet
DNA Bank Top

CSIV-TRE-RfA-UbC-KT (#RDB12878)

Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by UbC promoter.


Drawn by SnapGene® software
Sequence (full) RDB12878hts02.seq
Remarks, protocol and/or map (pdf) RDB12878.pdf
Publication Kurita, R., PLoS One 8 (3): e59890 (2013). [link to RRC of NBRP]
Test sheet RDB12878_A7Hhp1-4.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by UbC promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSIV-TRE-RfA-UbC-KT (Plasmid)
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12878 CSIV-TRE-RfA-UbC-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSIV-TRE-RfA-UbC-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12878).

Reference section:

Kurita, R., Suda, N., Sudo, K., Miharada, K., Hiroyama, T., Miyoshi, H., Tani, K., Nakamura, Y., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656. [link to RRC of NBRP]
reference Noura, M., Suppression of super-enhancer-driven TAL1 expression by KLF4 in T-cell acute lymphoblastic leukemia.
29 June 2023, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-3069900/v1
reference Jeong, H.C., Timely Degradation of Wip1 Phosphatase by APC/C Activator Protein Cdh1 is Necessary for Normal Mitotic Progression. J. Cell. Biochem. 116 (8): 1602-1612 (2015). PMID 25649870.
reference Murata, K., Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis. Biochem. Biophys. Res. Commun. 458 (3): 470-475 (2015). PMID 25660450.
reference Goshima, T., Mammal-specific H2A variant, H2ABbd, is involved in apoptotic induction via activation of NF-κB signaling pathway. J. Biol. Chem. 289 (17): 11656-11666 (2014). PMID 24584930.
user_report Wu, W., Ribosomal S6 kinase (RSK) plays a critical role in DNA damage response via the phosphorylation of histone lysine demethylase KDM4B. Breast Cancer Res. 26 (1): 146 (2024). PMID 39434131. [link to RRC of NBRP]
user_report Qi, F., The ribonuclease domain function is dispensable for SLFN11 to mediate cell fate decision during replication stress response. Genes Cells 28 (9): 663-673 (2023). PMID 37469008. [link to RRC of NBRP]
user_report Alvi, E., Mouse Slfn8 and Slfn9 genes complement human cells lacking SLFN11 during the replication stress response. Commun. Biol. 6 (1): 1038 (2023). PMID 37833372. [link to RRC of NBRP]
user_report Kubota, H., RUNX inhibitor suppresses graft-versus-host disease through targeting RUNX-NFATC2 axis. eJHaem. 2 (3): 449-458 (2021). PMID 35844683. [link to RRC of NBRP]
user_report Noura, M., TXNIP induces growth arrest and enhances ABT263-induced apoptosis in mixed-lineage leukemia-rearranged acute myeloid leukemia cells. FEBS Open Bio. 10 (8): 1532–1541 (2020). PMID 32511893. [link to RRC of NBRP]
user_report Noura, M., Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells. Sci. Rep. 10 (1): 20245 (2020). PMID 33219287. [link to RRC of NBRP]
user_report Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]
user_report Sato, K., FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5'-DNA end. Nucleic Acids Res. 44 (22): 10758-10771 (2016). PMID 27694619. [link to RRC of NBRP]