Resource data sheet
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CSIV-TRE-RfA-UbC-KT

Backbone vector of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by UbC promoter.

Catalog number RDB12878
Resource name CSIV-TRE-RfA-UbC-KT
Clone info. Backbone vector of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by UbC promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-UbC-KT (Plasmid)
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12878 CSIV-TRE-RfA-UbC-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12878z.seq from Depositor
Electronic file PDF RDB12878.pdf from Depositor

Featured content

References

original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12878_A5B5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_N_rev RDB12878_A5B5a_2.seq
>12878_12878_A5B5_Cm_N_rev_E05_14.ab1
    1 CTATCGCAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT
   61 TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ACGGATCCTA ACTCAAAATC
  121 CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGCGGCCGCC
  181 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT
  241 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTT TTGTACAAAC
  301 TTGTGATGCT AGCGGCGATC TGACGGTTCA CTAAACGAGC TCTGCTTATA TAGGCCTCCC
  361 ACCGTACACG CCTACCTCGA CATACGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA
  421 CGTTCTCTAT CACTGATAGG GATAAACTCG ACATACGTTC TCTATCACTG ATAGGGAGTA
  481 AACTCGACAT ACGTTCTCTA TCACTGATAG GGAGTAAACT CGACATACGT TCTCTATCAC
  541 TGATAGGGAG TAAACTCGAC ATCGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG
  601 TTCTCTATCA CTGATAGGGA GTAAACTCGA TTGAAGACGA AAGGGCCGAA TTCAAAGTGA
  661 ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA
  721 TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC
  781 CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT GCCATTTGTA GATCCGTTCA
  841 CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC TTCTTCTATT CCTTCGGGCC
  901 TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA TAATGGTGAA TATCCCTGCC
  961 TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT TAAACCCTAC CAAGCCTCCT
 1021 ACTATCATTA TGAATAATTT TATATACCAC AGCCCAATTG TATGTTAAAA CATCACAAAC
 1081 TGCCCATTAT CTAATTCAAT AATCCTTGCT TCATCGTTTC CTGCGTGGCT TTTTGGCCGA
1141 ATC
//
Primer: Cm_C_F RDB12878_A5B5b_2.seq
>12878_12878_A5B5_Cm_C_F_F05_17.ab1
    1 GGCCAGCGAG ATCGGCTTAC TAAAGCCAGA TAACAGTATG CGTATCTCTG CGCGCTGATT
   61 TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTATGC
  121 TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA
  181 TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG
  241 CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT
  301 GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGGCTGGT GAAATGCAGT TTAAGGTTTA
  361 CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA
  421 CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT
  481 CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC
  541 CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG
  601 CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC
  661 CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT
  721 ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT
  781 ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTGATGCT ACCGGTCTCG AGATCGGGAG
  841 ATCTGGCCTC CGCGCCGGGT TTTGGCGCCC CCCGCGGGCG CCCCCCCTCC TCACGGCGAG
  901 CGCTGCCACG TCAGACGAAG GGCGCAGCGA GCGTCCTGAT CCTTCCGCCC GGACGCTCAG
  961 GACAGCGGCC CGCTGCTCAT AAGACTCGGG CCTTTAGAAC CCCAGTATCA GCAGATGGAC
 1021 ATTTTAGGAC GGGACTTTGG GTGACTCTAG GCACTGGCTT TTCTTTCAGA GAGCGGACAG
 1081 CGAGAAAGTA GTCGCTCTCG CGAATTCTGC GAAGGCATCT CCGTTGGGGG CCGGCTGAAT
1141 C
//
Primer: UbCpro_F RDB12878_A5B5c_2.seq
>12878_12878_A5B5_UbCpro_F_G05_20.ab1
    1 TGCAATGCAT TTCAGTGTTA GACTAGTAAA TTGTCCGCTA AATTCTGGCC GTTTTTGGCT
   61 TTTTTGTTAG ACCGGACCGT GTTTAGCGCT ACCGGTCCAC CATGGTGAGC GTGATCAAGC
  121 CCGAGATGAA GATGAAGTAC TTCATGGACG GCAGCGTGAA CGGCCACGAG TTCACCGTGG
  181 AGGGCGAGGG CACCGGCAAG CCCTACGAGG GCCACCAGGA GATGACCCTG AGGGTGACAA
  241 TGGCCAAGGG CGGCCCCATG CCCTTCAGCT TCGACCTGGT GAGCCACACC TTCTGCTACG
  301 GCCACAGGCC CTTCACCAAG TACCCCGAGG AGATCCCCGA CTACTTCAAG CAGGCCTTCC
  361 CCGAGGGCCT GAGCTGGGAG AGGAGCCTCC AGTTCGAGGA CGGCGGCTTC GCCGCCGTGA
  421 GCGCCCACAT CAGCCTGAGG GGCAACTGCT TCGAGCACAA GAGCAAGTTC GTGGGCGTGA
  481 ACTTCCCCGC CGACGGCCCC GTGATGCAGA ACCAGAGCAG CGACTGGGAG CCCAGCACCG
  541 AGAAGATCAC CACCTGCGAC GGCGTGCTGA AGGGCGACGT GACCATGTAC CTGAAGCTGG
  601 CCGGCGGCGG CAACCACAAG TGCCAGTTCA AGACCACCTA CAAGGCCGCC AAGAAGATCC
  661 TGAAGATGCC CCAGAGCCAC TTCATCGGCC ACAGGCTGGT GAGGAAGACC GAGGGCAACA
  721 TCACCGAGCT GGTGGAGGAC GCCGTGGCCC ACTGCGAGGG CAGAGGAAGT CTTCTAACAT
  781 GCGGTGACGT GGAGGAGAAT CCCGGCCCTA TGTCTAGACT GGACAAGAGC AAAGTCATAA
  841 ACGGCGCTCT GGAATTACTC AATGGAGTCG GTATCGAAGG CCTGACGACA AGGAAACTCG
  901 CTCAAAAGCT GGGAGTTGAG CAGCCTACCC TGTACTGGCA CGTGAAGAAC AAGCGGGGCC
  961 CTGCTCGATG CCCTGCCAAT CGAGATGCTG GGACAGGCAT CATACCCCAC TTCTGCCCCC
 1021 TGCAGCGAGT CATGGCAGAC TTCTGCGACA CGCAGTCATC CGCTGTGCTC TCTCTCACAT
1081 CGCGACGACT ATGTGCATCT CGTCCCGGCC AACCGAGAAA CCAGTACGAA ACCCAG
//
Primer: hKO_F RDB12878_A5B5d_2.seq
>12878_12878_A5B5_hKO_F_D05_11.ab1
    1 CCTACCGGCA AGGCTGGTGA GGAGACCGAG GGCAACATCA CCGAGCTGGT GGAGGACGCC
   61 GTGGCCCACT GCGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTGGA GGAGAATCCC
  121 GGCCCTATGT CTAGACTGGA CAAGAGCAAA GTCATAAACG GCGCTCTGGA ATTACTCAAT
  181 GGAGTCGGTA TCGAAGGCCT GACGACAAGG AAACTCGCTC AAAAGCTGGG AGTTGAGCAG
  241 CCTACCCTGT ACTGGCACGT GAAGAACAAG CGGGCCCTGC TCGATGCCCT GCCAATCGAG
  301 ATGCTGGACA GGCATCATAC CCACTTCTGC CCCCTGGAAG GCGAGTCATG GCAAGACTTT
  361 CTGCGGAACA ACGCCAAGTC ATTCCGCTGT GCTCTCCTCT CACATCGCGA CGGGGCTAAA
  421 GTGCATCTCG GCACCCGCCC AACAGAGAAA CAGTACGAAA CCCTGGAAAA TCAGCTCGCG
  481 TTCCTGTGTC AGCAAGGCTT CTCCCTGGAG AACGCACTGT ACGCTCTGTC CGCCGTGGGC
  541 CACTTTACAC TGGGCTGCGT ATTGGAGGAA CAGGAGCATC AAGTAGCAAA AGAGGAAAGA
  601 GAGACACCTA CCACCGATTC TATGCCCCCA CTTCTGAGAC AAGCAATTGA GCTGTTCGAC
  661 CGGCAGGGAG CCGAACCTGC CTTCCTTTTC GGCCTGGAAC TAATCATATG TGGCCTGGAG
  721 AAACAGCTAA AGTGCGAAAG CGGCGGGCCG GCCGACGCCC TTGACGATTT TGACTTAGAC
  781 ATGCTCCCAG CCGATGCCCT TGACGACTTT GACCTTGATA TGCTGCCTGC TGACGCTCTT
  841 GACGATTTTG ACCTTGACAT GCTCCCCGGG TAACTAAGTA AGGATCCTCT AGAGTTAACA
  901 TCGAGGGATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC
  961 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT ATTGCCTTTT
 1021 GTATCATGCT ATGCTCCGTA TGGCTTTCAT TTTCTCCTTC TGGTATAATC TGCTGGCTGT
1081 CTCGTTATGA GAGTGTGCCC GTGTCAAGCA CGTGACCGTG CTGGTTGA
//
Primer: CMV-Forward RDB12878_A5B5e_2.seq
>12878_12878_A5B5_CMV-Forward_D12_12.ab1
    1 CGGACCCACC CCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG
  121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC
  181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT
  241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG
  301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG
  361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG
  421 AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT
  481 TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG
  541 GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA
  601 AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA
  661 TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA
  721 AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC
  781 CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAAG TGAATTATAT
  841 AAATATAAAG TAGTAAAAAA TTGAAACCAT TTAGGAGTAG CACCCACCAA GGCAAAAGAG
  901 AAGAGTGGGT GCAGAGAGAA AAAAAGAGCA GTGGGGAATA GGAGCTTTTG TTCCCTTGGG
  961 GTTTCTTTGG GAAGCAGCAG GAAAGCACTA TTGGGGCGCA AGCGGTTCAA TGAACGCTGA
 1021 CGGTTACCAG GCCCAGACAA TTATTTGGTC CTGGTATTAG CTGCCAGCCA AGCCAGAACC
1081 AATTTTGGCC
//
Primer: BGH_rev RDB12878_A5B5f_2.seq
>12878_12878_A5B5_BGHrev_E12_15.ab1
    1 CGATGTAGGG GGTCTGCGAG AGATTTTCCG CACTGACTAA AAGGGTCTGA GGGATCTCTA
   61 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA
  121 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC
  181 TAACCAGAGA GACCCAGTAC AGTCCGGAAT AACTTCGTAT AATGTATGCT ATACGAAGTT
  241 ATGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT TTCTTTTAAA
  301 AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC CTGAGGTGTG
  361 ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC AGCATTGGTA
  421 GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG GTATCGATGC
  481 GGGGAGGCGG CCCAAAGGGA GATCCGACTC GTCTGAGGGC GAAGGCGAAG ACGCGGAAGA
  541 GGCCGCAGAG CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG CTGGATTGAG GGCCGAAGGG
  601 ACGTAGCAGA AGGACGTCCC GCGCAGAATC CAGGTGGCAA CACAGGCGAG CAGCCATGGA
  661 AAGGACGTCA GCTTCCCCGA CAACACCACG GAATTGTCAG TGCCCAACAG CCGAGCCCCT
  721 GTCCAGCAGC GGGCAAGGCA GGCGGCGATG AGTTCCGCCG TGGCAATAGG GAGGGGGAAA
  781 GCGAAAGTCC CGGAAAGGAG CTGACAGGTG GTGGCAATGC CCCAACCAGT GGGGGTTGCG
  841 TCAGCAAACA CAGTGCACAC CACGCCACGT TGCCTGACAA CGGGGCCACA ACTCCTCATA
  901 AAGAGACAGC AACCAGGATT TATACAAGGG AGGGAGAAAA TGAAAGCCAT ACGGGGAAAG
  961 CAATAGCATG ATACAAAGGG CATTTAAAGG CAGCGTATCC CACATAGCGT AAAAAGGAGC
 1021 AACATAGGTT TAAGATACCC AGTCAAATCC TTTCACAATT TTGTAAATCA GAAGTTGGAA
1081 TTATCGGATA AGCTTGAATC CCCTTCGAAT GT
//
Primer: SV40pro_F_V2 RDB12878_A5B5g_2.seq
>12878_12878_A5B5_SV40pro_F_V2_F12_18.ab1
    1 GTTTGCAATC TTTTTGGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGGA GCTTGTATAT
   61 CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT
  121 AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT
  181 GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC
  241 CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT
  301 CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG
  361 CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC
  421 CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA
  481 CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA
  541 TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC
  601 CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT
  661 GTTTATTGCA GGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAA TTTCACAAAT
  721 AAAGCATTTT TTTCACTGCA TTTCTAGTTT GTGGTTTTGT CCAAACTCAT CAATGTATCT
  781 TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG
  841 TTTCCTGTGT GAAAATTGTT ATCCGCTCAC AAATTCCCAC ACAACATACG AGCCCGGGAA
  901 GCATAAAAGT GTAAAAGCCC TGGGGGTTGC CCTAAATGGA GTGAGCTAAC TTCACAATTT
  961 AAATTTGCCG GTTTGCCGGC TCACCTGCCC CGGCCTTTTC CCAGGTCCGG GGAAACCCTT
 1021 GTCGGTTGCC CAGCCCTGCA ATTTAAAATG AATTCCGGGC CCAAACGCCG CGGGGGAAAG
 1081 GCCGGTTGGC GTAATTGGGC CGCCTCATCC GGCTATCCCA TCCGTCTCCA ACCTGAACCT
1141 CTCGCCCGTA TGTGCAGCGC T
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.
reference Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.
reference Jeong, H.C., Timely Degradation of Wip1 Phosphatase by APC/C Activator Protein Cdh1 is Necessary for Normal Mitotic Progression. J. Cell. Biochem. 116 (8): 1602-1612 (2015). PMID 25649870.
reference Murata, K., Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis. Biochem. Biophys. Res. Commun. 458 (3): 470-475 (2015). PMID 25660450.
reference Goshima, T., Mammal-specific H2A variant, H2ABbd, is involved in apoptotic induction via activation of NF-κB signaling pathway. J. Biol. Chem. 289 (17): 11656-11666 (2014). PMID 24584930.

2018.10.09

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