Resource data sheet
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CSIV-TRE-GFP-UbC-KT

Lentivirus vector construct of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by UbC promoter.

Catalog number RDB12877
Resource name CSIV-TRE-GFP-UbC-KT
Clone info. Lentivirus vector construct of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by UbC promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-UbC-KT ()
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
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Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12877 CSIV-TRE-GFP-UbC-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12877z.seq from Depositor
Electronic file PDF RDB12877.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12877_A5D2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N RDB12877_A5D2a.seq
>12877_12877_A5D2_2_EGFP-N_A08_02.ab1
    1 GAACCCGGGA TGACGCTCCT CGCCCTTGCT CACCATGGTG GAGCCTGCTT TTTTGTACAA
   61 ACTTGTGATG CTAGCGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA TATAGGCCTC
  121 CCACCGTACA CGCCTACCTC GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA
  181 TACGTTCTCT ATCACTGATA GGGATAAACT CGACATACGT TCTCTATCAC TGATAGGGAG
  241 TAAACTCGAC ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC GTTCTCTATC
  301 ACTGATAGGG AGTAAACTCG ACATCGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA
  361 CGTTCTCTAT CACTGATAGG GAGTAAACTC GATTGAAGAC GAAAGGGCCG AATTCAAAGT
  421 GAATTTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA TTTTTGTAAT TTGTTTTTGT
  481 AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA TTCTTTCCCC TGCACTGTAC
  541 CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA CTGCCATTTG TAGATCCGTT
  601 CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT TCTTCTTCTA TTCCTTCGGG
  661 CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC GATAATGGTG AATATCCCTG
  721 CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT CTTAAACCTA CCAAGCCTCC
  781 TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT GTTATGTTAA ACCAATTCCA
  841 CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA TTCTTTTCTT GCTGGTTTTG
  901 CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG TTAATTTCTC TGTCCCACTC
  961 CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA TTACTCCCAA CTAGCATTCC
 1021 AAGGCACAGC AGTGGTGCAA TGAGTTTTCC AGAGCACCCC AATCCCCAGG AGCTGTGATT
 1081 CTTAGTATCT TTCACAGCCA GGAATCTGCT TGAGCTGCCT GATGCCCCAG ACTGTGAGTT
1141 GCAACAGAAT GCCTGTTTTG CGG
//
Primer: EGFP-C RDB12877_A5D2b.seq
>12877_12877_A5D2_2_EGFP-C_B08_05.ab1
    1 CCGGTTTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCCTACT AGTAGGATCT
   61 CGAGATATCT AGACCCAGCT TTCTTGTACA AAGTGGTGAT GCTACCGGTC TCGAGATCGG
  121 GAGATCTGGC CTCCGCGCCG GGTTTTGGCG CCCCCCGCGG GCGCCCCCCT CCTCACGGCG
  181 AGCGCTGCCA CGTCAGACGA AGGGCGCAGC GAGCGTCCTG ATCCTTCCGC CCGGACGCTC
  241 AGGACAGCGG CCCGCTGCTC ATAAGACTCG GCCTTAGAAC CCCAGTATCA GCAGAAGGAC
  301 ATTTTAGGAC GGGACTTGGG TGACTCTAGG GCACTGGTTT TCTTTCCAGA GAGCGGAACA
  361 GGCGAGGAAA AGTAGTCCCT TCTCGGCGAT TCTGCGGAGG GATCTCCGTG GGGCGGTGAA
  421 CGCCGATGAT TATATAAGGA CGCGCCGGGT GTGGCACAGC TAGTTCCGTC GCAGCCGGGA
  481 TTTGGGTCGC GGTTCTTGTT TGTGGATCGC TGTGATCGTC ACTTGGTGAG TAGCGGGCTG
  541 CTGGGCTGGC CGGGGCTTTC GTGGCCGCCG GGCCGCTCGG TGGGACGGAA GCGTGTGGAG
  601 AGACCGCCAA GGGCTGTAGT CTGGGTCCGC GAGCAAGGTT GCCCTGAACT GGGGGTTGGG
  661 GGGAGCGCAG CAAAATGGCG GCTGTTCCCG AGTCTTGAAT GGAAGACGCT TGTGAGGCGG
  721 GCTGTGAGGT CGTTGAAACA AGGTGGGGGG CATGGTGGGC GGCAAGAACC CAAGGTCTTG
  781 AGGCCTTCGC TAATGCGGAA AGCTCTTATT CGGTGAGATG GGCTGGGGCA CATCTGGGGA
  841 CCCTGACGTG AGTTTGTCAC TGACTGAGAC TCGGTTGTCG TCTGTTGCGG GGGCGCAGTA
  901 TGCGTGCGTG GGCCAGTGCC ACCCGTACTT GGGAGCGCGG CGCCTCGGTC TGTCTGACGT
  961 CAACGTTCGT GCTTAATGCA GGTGGGCCAC TGCGTAAGTT ACGATAGCTA TCCGTCCAGA
1021 CAGTCGACTA GTAGCTTCTG ATTC
//
Primer: UbCpro_F RDB12877_A5D2c.seq
>12877_12877_A5D2_2_UbCpro_F_C08_08.ab1
    1 GCATTGTACT TCAGTGTTAG ACTAGTAAAT TGTCCGCTAA ATTCTGGCCG TTTTTGGCTT
   61 TTTTGTTAGA CCGGACCGTG TTTAGCGCTA CCGGTCCACC ATGGTGAGCG TGATCAAGCC
  121 CGAGATGAAG ATGAAGTACT TCATGGACGG CAGCGTGAAC GGCCACGAGT TCACCGTGGA
  181 GGGCGAGGGC ACCGGCAAGC CCTACGAGGG CCACCAGGAG ATGACCCTGA GGGTGACAAT
  241 GGCCAAGGGC GGCCCCATGC CCTTCAGCTT CGACCTGGTG AGCCACACCT TCTGCTACGG
  301 CCACAGGCCC TTCACCAAGT ACCCCGAGGA GATCCCCGAC TACTTCAAGC AGGCCTTCCC
  361 CGAGGGCCTG AGCTGGGAGA GGAGCCTCCA GTTCGAGGAC GGCGGCTTCG CCGCCGTGAG
  421 CGCCCACATC AGCCTGAGGG GCAACTGCTT CGAGCACAAG AGCAAGTTCG TGGGCGTGAA
  481 CTTCCCCGCC GACGGCCCCG TGATGCAGAA CCAGAGCAGC GACTGGGAGC CCAGCACCGA
  541 GAAGATCACC ACCTGCGACG GCGTGCTGAA GGGCGACGTG ACCATGTACC TGAAGCTGGC
  601 CGGCGGCGGC AACCACAAGT GCCAGTTCAA GACCACCTAC AAGGCCGCCA AGAAGATCCT
  661 GAAGATGCCC CAGAGCCACT TCATCGGCCA CAGGCTGGTG AGGAAGACCG AGGGCAACAT
  721 CACCGAGCTG GTGGAGGACG CCGTGGCCCA CTGCGAGGGC AGAGGAAGTC TTCTAACATG
  781 CGGTGACGTG GAGGAGAATC CCGGCCCTAT GTCTAGACTG GACAAGAGCA AAGTCATAAA
  841 CGGCGCTCTG GAATTACTCA ATGGAGTCGG TATCGAAGGC CTGACGACAA GGAAACTCGC
  901 TCAAAAGCTG GGAGTTGAGC AGCCTACCCT GTACTGGCAC GTGAAGAACA AGCGGGCCCT
  961 GCTCGATGCC CTGCCAATCG AGATGCTGGA CAGGCATCAT ACCCACTTTC TGCCCCCTGG
 1021 AAGCGAGTCA TGGCAAGACT TTTCTGCGGA ACACGCCAGT CATTCCGCTG TGCTCCTCCT
 1081 CTCCACATCG CGACGGGGCT AAGTGCATCT CGCACCGGCC CAACAGAGAA ACAGTACGAA
1141 ACCCTGAAAA ATTCAGCCTT CCGCCGCGTT TCCCATG
//
Primer: hKO_F RDB12877_A5D2d.seq
>12877_12877_A5D2_2_hKO_F_D08_11.ab1
    1 CAACACGGGC CAGGCTGGTG AGGAGACCGA GGGCAACATC ACCGAGCTGG TGGAGGACGC
   61 CGTGGCCCAC TGCGAGGGCA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC
  121 CGGCCCTATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC GGCGCTCTGG AATTACTCAA
  181 TGGAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA
  241 GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA
  301 GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT
  361 TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA
  421 AGTGCATCTC GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC
  481 GTTCCTGTGT CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG
  541 CCACTTTACA CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG
  601 AGAGACACCT ACCACCGATT CTATGCCCCC ACTTCTGAGA CAAGCAATTG AGCTGTTCGA
  661 CCGGCAGGGA GCCGAACCTG CCTTCCTTTT CGGCCTGGAA CTAATCATAT GTGGCCTGGA
  721 GAAACAGCTA AAGTGCGAAA GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTAGA
  781 CATGCTCCCA GCCGATGCCC TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT
  841 TGACGATTTT GACCTTGACA TGCTCCCCGG GTAACTAAGT AAGGATCCTC TAGAGTTAAC
  901 ATCGAGGGAT CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA
  961 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT
 1021 TTGTATCATG CTATTGCTTC CCGTATGGGC TTTCATTTTC TCTCTTGTAT AAATCTGCTG
 1081 CCTGTCTCTT ATGAAGAAGT TGTGATCGTG TCAGGCAACG TGGCGTTGCT GTGCACTGGT
1141 GGTTTTGCTG AACGCAA
//
Primer: CMV-Forward RDB12877_A5D2e.seq
>12877_12877_A5D2_2_CMV-Forward_E08_14.ab1
    1 CGGATCACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
  721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGCGGCCGCT
  781 GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA
  841 TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAGTGGT
  901 GCAGAGAGAA AAAAGAGCAG TGGGAATAGG AGCTTTGTTC CTTGGGTTCT TGGGAGCAGC
  961 AGGAAGCACT ATGGGCGCAG CGTCAATGAC GCTGACGGTA CAGGGCCAGA CAATTTATTG
 1021 TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGCTAT TGAGGCGCCA CAGCATCTGT
 1081 TGCAACTCAC AGTCCTGGGC ATCCAGGCCA GCCTCCAGGC AAGAATTCCC TGGGCCTGTG
1141 TTGGAAG
//
Primer: BGH_rev RDB12877_A5D2f.seq
>12877_12877_A5D2_2_BGHrev_F08_17.ab1
    1 CGAGTCGGGA ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
   61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  181 ACCAGAGAGA CCCAGTACAG TCCGGAATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT
  241 GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT CCCCCCTTTT CTTTTAAAAA
  301 GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT AAAGGTACCT GAGGTGTGAC
  361 TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG GCACAATCAG CATTGGTAGC
  421 TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG TCTCGACGGT ATCGATGCGG
  481 GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGAGGGCGA AGGCGAAGAC GCGGAAGAGG
  541 CCGCAGAGCC GGCAGCAGGC CGCGGGAAGG AAGGTCCGCT GGATTGAGGG CCGAAGGGAC
  601 GTAGCAGAAG GACGTCCCGC GCAGAATCCA GGTGGCAACA CAGGCGAGCA GCCATGGAAA
  661 GGACGTCAGC TTCCCCGACA ACACCACGGA ATTGTCAGTG CCCAACAGCC GAGCCCCTGT
  721 CCAGCAGCGG GCAAGGCAGG CGGCGATGAG TTCCGCCGTG GCAATAGGGA GGGGGAAAGC
  781 GAAAGTCCCG GAAAGGAGCT GACAGGTGGT GGCAATGCCC CAACCAGTGG GGGTTGCGTC
  841 AGCAAACACA GTGCACACCA CGCCACGTTG CCTGACAACG GGCCACAACT CCTCATAAAG
  901 AGACAGCAAC CAGGATTTAT ACAAGGAGGA GAAAATGAAA GCCATACGGG AAGCAATAGC
  961 ATGATACAAA GGCATTAAAG CAGCGTATCC ACATAGCGTA AAAGGAGCAC ATAGTTAAGA
 1021 ATACCAGTCA TCTTTCACAA ATTTTGTAAT CCAGAGGTGG ATATCGATAG CTGATCCCTC
 1081 GATGTACTCT AGAGATCTTA CCTAGTACCT GGGAGCATGT CAAGTCCAAT CGTCCAGAGC
1141 GTTCAGGCAA GGGCAAGCAA TTATTCCAAG GGGT
//
Primer: SV40pro_F RDB12877_A5D2g.seq
>12877_12877_A5D2_2_SV40pro_F_G08_20.ab1
    1 CCCGGGTGCT GGGCCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
  121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
  181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
  241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
  301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
  361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
  421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
  481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
  541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
  661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
  721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
  781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
  841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
  901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
  961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
 1021 TTAATGAATC GGGCCAACGC GCGGGGAGAG GCGGCTTGCG TATTGGCGCT CTTCCGCCTT
 1081 CTCGCTCACT GACTCGCTGC GCCTCGATCG TTCGGCTGCG CGAGCGGTAT CAGCTCACCT
1141 CCAAAGGGCG GTAATTACCG GCA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.09

GNP_filter3_RDBDEP_html_180823.pl