RDB12876 - CSIV-TRE-RfA-CMV-KT

Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by CMV promoter.

Distribution information

Sequence	RDB12876zzt.seq
Remarks, protocol and/or map (pdf)	RDB12876.pdf

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内)

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的提供条件：利用者は、hKO1蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。宮脇敦史博士による提供承諾書 [Word]
備考	【追加提供依頼書】受入れ確認書(書式J) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。宮脇敦史博士の書面による提供承諾が必要です。

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QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12876	CSIV-TRE-RfA-CMV-KT	DNA solution

Featured content	Lentivirus Vector (English text)
Featured content	Lentivirus Vector (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

original	Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123.
reference	Umei, T.C., Single-Construct Polycistronic Doxycycline-Inducible Vectors Improve Direct Cardiac Reprogramming and Can Be Used to Identify the Critical Timing of Transgene Expression. Int. J. Mol. Sci. 18 (8). pii: E1805 (2017). PMID 28825623.
reference	Chapnick, D.A., Leader cell positioning drives wound-directed collective migration in TGFβ-stimulated epithelial sheets. Mol. Biol. Cell, 25 (10): 1586-1593 (2014). PMID 24623725.
reference	Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.
reference	Tai, M.C., miR-342-3p regulates MYC transcriptional activity via direct repression of E2F1 in human lung cancer. Carcinogenesis 36 (12): 1464-1473 (2015). PMID 26483346.
reference	Lu, J., Expression of N-Acetylglucosaminyltransferase III Suppresses α2,3-Sialylation, and Its Distinctive Functions in Cell Migration Are Attributed to α2,6-Sialylation Levels. J. Biol. Chem. 291 (11): 5708-5720 (2016). PMID 26801611.
user_report	Zhang, H., The maturation of iPS cell-derived brain microvascular endothelial cells by inducible-SOX18 expression. Fluids Barriers CNS 20 (1): 10 (2023). PMID 36732767.
user_report	Orimoto, A., Establishment of human airway epithelial cells with doxycycline-inducible cell growth and fluorescence reporters. Cytotechnology 73 (4): 555-569 (2021). PMID 34349346.
user_report	Ito, N., Direct reprogramming of fibroblasts into skeletal muscle progenitor cells by transcription factors enriched in undifferentiated subpopulation of satellite cells. Sci. Rep. 7 (1): 8097 (2017). PMID 28808339.

Primer: KO-M_R (Pr0821) Region: hKO1 5', CMV pro2 Sequence file: RDB12876_A4Kla.seq
>12876_12876_B0BiS_KO-M_R1_A06_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNTGGNNGCT CCTCTCCNNG CTCNGGCCCT CGGGGAAGGC 61 CTGCTTGAAG TAGTCGGGGN NCTCCTCGGG GTACTTGGTG AAGGGCCTGT GGCCGTAGCA 121 GAAGGTGTGG CTCACCAGGT CGAAGCTGAA GGGCATGGGG CCGCCCTTGG CCATTGTCAC 181 CCTCAGGGTC ATCTCCTGGT GGCCCTCGTA GGGCTTGCCG GTGCCCTCGC CCTCCACGGT 241 GAACTCGTGG CCGTTCACGC TGCCGTCCAT GAAGTACTTC ATCTTCATCT CGGGCTTGAT 301 CACGCTCACC ATGGTGGACC GGTGAATTCG GGGCCGCGGA GGCTGGATCG GTCCCGGTGT 361 CTTCTATGGA GGTCAAAACA GCGTGGATGG CGTCTCCAGG CGATCTGACG GTTCACTAAA 421 CGAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG 481 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCNAAA CAAACTCCCA 541 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA 601 TTGATGTACT GCCNAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC 661 TGCCNAGTAG GAAAGTCCCA TAANGTCATG TACTGGGCAT AATGNCAGGC GGGCCATTTA 721 CCGTCATTGA CGTCAATANG GGGCGTACTN NGCATATGAT ACACTTGATG TACTGNNNAN 781 NGGGCAGTTT ACCGNAAATA CTCCACCNNT NNNCNTCNNN GGNAAGNNCN NATNNNNNTT 841 ACTATGGGNN NNTACNNCNN TNNNTNGANN NNNNNNGGCN GGGGNNNNNN GGCNNNCANC 901 CNNNNNGGNC NNNNNNNNNN //
Primer: hKO_F (Pr0157) Region: T2A, rtTA-Ad Sequence file: RDB12876_A4Klb.seq
>12876_12876_A4Kl_2_hKO_F_A10_01.ab1 1 NNNNNNNNNN NNNGCNNGNN NNGANNNCGA NGGCAACATC ACNNNNNNGG TGGAGGANGC 61 CGTGGCCCAC TGNNNNNNCA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC 121 CGGCCCTATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC GGCGCTCTGG AATTACTCAA 181 TGGAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA 241 GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA 301 GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT 361 TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA 421 AGTGCATCTC GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC 481 GTTCCTGTGT CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG 541 CCACTTTACA CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG 601 AGAGACACCT ACCACCGATT CTATGCCCCC ACTTCTGAGA CAAGCAATTG AGCTGTTCGA 661 CCGGCAGGGA GCCGAACCTG CCTTCCTTTT CGGCCTGGAA CTAATCATAT GTGGCCTGGA 721 GAAACAGCTA AAGTGCGAAA GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTANA 781 CATGCTCCCA GCCGATGCCC TTGNCGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT 841 TGACGANTTT GACCTTGACA TGCTCCCCGG GNAACTAAGT AANGNTCCTC TAGAGTTAAC 901 ATCNAGGGNT CNAGCTTATC GATAATCAAC NNCNNGATTA CNAAANTNGN NAAANANTGN 961 CNGGNANNNN NNNNTATGNT GNNNNNTTTN NNNNNNNNN //
Primer: Cm_N_rev (Pr0014) Region: attR1, tight TRE pro, cPPT_CTS Sequence file: RDB12876_A4Klc.seq
>12876_12876_A4Kl_2_Cm_N_rev_B10_04.ab1 1 NNNNNNNNNN NNTNTTNNNG NNGCNNTTGG GNNNTATCAA CNNNNNNATA TCCNGTGATT 61 TTTTTCTCCN NNNNNNNNTC CTTAGCTCCT GAAAATCTCG ACGGATCCTA ACTCAAAATC 121 CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGCGGCCGCC 181 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT 241 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTT TTGTACAAAC 301 TTGTGATGCT AGCGGCGATC TGACGGTTCA CTAAACGAGC TCTGCTTATA TAGGCCTCCC 361 ACCGTACACG CCTACCTCGA CATACGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA 421 CGTTCTCTAT CACTGATAGG GATAAACTCG ACATACGTTC TCTATCACTG ATAGGGAGTA 481 AACTCGACAT ACGTTCTCTA TCACTGATAG GGAGTAAACT CGACATACGT TCTCTATCAC 541 TGATAGGGAG TAAACTCGAC ATCGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG 601 TTCTCTATCA CTGATAGGGA GTAAACTCGA TTGAAGACGA AAGGGCCGAA TTCAAAGTGA 661 ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA 721 TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC 781 CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGANTACT GCCATTTGTA GATCCGTTCA 841 CTAATCNAAT GGATCTNNCT CTGNCTCTCT CTNCNCCTTC NNCNNNTATT NCNNCNGNNC 901 TGTCNGNNCC CNNNNGGNNN NGNANGNNGN NNTGAANCNA NNNNGGNNNN NNTNCNNNNN 961 NNANTNNNNN NNNNN //
Primer: CS-CMVen_upR (Pr0822) Region: attR2, ccdB Sequence file: RDB12876_A4Kld.seq
>12876_12876_B0BiS_CS-CMVen_upR_B06_02_ABI08.ab1 1 NNNNNNNNNN NNNANNGANN NGGNNNNNNN NTATGCAATG GGCCNAGCTC CTCGAGACCG 61 GTAGCATCAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA 121 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA 181 TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT 241 CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC 301 ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG 361 CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA 421 CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT 481 ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATT 541 TCACCAGCCC CTGTTCTCGT CAGCAAAAGA GCCGTTCATT TCAATAAACC GGGCGACCTC 601 AGCCATCCCT TCCTGATTTT CCGCTTTCCA GCGTTCGGCA CGCAGACGAC GGGCTTCATT 661 CTGCATGGTT GTGCTTACNN GACCGGANAT ATTGACATCA TATATGCCTT GANCAACTGA 721 TAGCTGTCGC TGTCAACTGT CACTGTAATA CGCTGCTTCN TANCATACCT CTTTTTGACA 781 TACTTCNGGT ATACNTATCA GTATATATTC NTATANCGCA AAAATCANCG CGCAAATACN 841 CATACNGNNN TCTGGNTTTN ANNNNNNCNG NNNNNNCGCN NTNNNNNNNN NN //
Primer: tTA-Ad R (Pr0542) Region: T2A, hKO1 3' Sequence file: RDB12876_A4Kle.seq
>12876_12876_B0BiS_tTA-Ad_R_C06_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT NNNNTCTTGT CNNGTCTAGA CATAGGGCCG 61 GGNTTCTCCT CCACGTCACC GCATGTTAGA AGACTTCCTC TGCCCTCGCA GTGGGCCACG 121 GCGTCCTCCA CCAGCTCGGT GATGTTGCCC TCGGTCTTCC TCACCAGCCT GTGGCCGATG 181 AAGTGGCTCT GGGGCATCTT CAGGATCTTC TTGGCGGCCT TGTAGGTGGT CTTGAACTGG 241 CACTTGTGGT TGCCGCCGCC GGCCAGCTTC AGGTACATGG TCACGTCGCC CTTCAGCACG 301 CCGTCGCAGG TGGTGATCTT CTCGGTGCTG GGCTCCCAGT CGCTGCTCTG GTTCTGCATC 361 ACGGGGCCGT CGGCGGGGAA GTTCACGCCC ACGAACTTGC TCTTGTGCTC GAAGCAGTTG 421 CCCCTCAGGC TGATGTGGGC GCTCACGGCG GCGAAGCCGC CGTCCTCGAA CTGGAGGCTC 481 CTCTCCCAGC TCAGGCCCTC GGGGAAGGCC TGCTTGAAGT AGTCGGGGAT CTCCTCGGGG 541 TACTTGGTGA AGGGCCTGTG GCCGTAGCAG AANGTGTGGC TCACCAGGTC GAAGCTGAAG 601 GGCATGGGGC CGCCCTTGGC CATTGTCACC CTCAGGGTCA TCTCCTGNTG GCCCTCGTAN 661 GGCTTGCCGG TGCCCTCGCC CTCCACGGTG AACTCGTGGC CGTTCACGCT GCCGTCCATG 721 AAGTACTTCA TCTTCATCTC GGGCTTGATC ACGCTCACNA TGGTGGNNCG GTGAATTCNG 781 GGCCGCGGNN GCTGGATCGG TCCCGGTGTC TTCTATGGAN GTCAAAACAG CGTGGATGGC 841 GTCTCCNNGC GATCTGANGG TTCACTNAAN NGANCTNTGN TNATATAGAN CNNCNNCCGN 901 ACNCGCNNNN CNNCNNNTTG NNNCNATGGG NNNGNANNNG NTNNNANNTT TNGGAANNTC 961 CNNNNNNNTT NNNNNNNNNA ANNNNNNNNC NNNNTNNNNN NNNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi, 5' LTR Sequence file: RDB12876_A4Klf.seq
>12876_12876_A4Kl_2_HIVgp_R1_F10_16.ab1 1 NNNNNNNNNT NNNNNNNNNC TTTTTTCTCT CTGCACCNCT CTNCNCTTTG CCTTGGTGGG 61 TGCTACTNNN NNNNNNNNAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT 121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGCTTGCTGT GCGGTGGTCT 181 TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA 241 TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG 301 ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT 361 CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT 421 TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA 481 TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG 541 TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG 601 CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT 661 TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGANGG ATCTCTAGTT 721 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG 781 ANGCTTAAGC AGTGGGTTCN CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA 841 CCAGANAGAC NNAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCNAC CNTACACGCC 901 NANCGCCCAT TTGCGTCAAT GGGGCGGANT NNNNACNANA TTTNNNAAAG TCCCNNNNAN 961 TTNNNNNNNN AAACAAANTC CNNTTNNNNN NNNNGGGGGN NNNNNNTNNN NNANNNNNNN 1021 NNN //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), 3'LTR, loxP, PRE Sequence file: RDB12876_A4Klg.seq
>12876_12876_B0BiS_BGH_rev2_D06_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNNGGNNN NNNACAGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA 121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG 181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC 241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGAATAA CTTCGTATAA 301 TGTATGCTAT ACGAAGTTAT GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT 361 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT 421 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG 481 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG 541 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGAGGGCGA 601 AGGCGAAGAC GCGGAAGAGG CCGCAGAGCC GGCAGCAGGC CGCGGGAAGG AAGGTCCGCT 661 GGATTGAGGG CCGAAGGGAC GTAGCAGAAG GACGTCCCGC GCAGAATCCA GGTGGCAACA 721 CAGGCGAGCA GCCATGGAAA GGACGTCAGC TTCCCCGACA ACACCACGGA ATTGTCAGTG 781 CCCAACAGCC GANCCCCTGT CCAGCAGCGG GCAAGGCAGG CGGCGANGAN TTCCGCCGNG 841 GCAATANGGA NGGGGGAAAG CGAAAGTCCC GGAAAGGANC NNANAGGNGG NGGCNNNGCC 901 CCNANCNNNG GGGGGTTGCG NCAGCAAACN CNNNGCNNAN NNNNNCNNNN NTNNNCNNGA 961 NNNNGGNNNN NNNNNN //
Primer: SV40pro_F (Pr0110) Region: Zeo, SV40 p(A) Sequence file: RDB12876_A4Klh.seq
>12876_12876_A4Kl_2_SV40pro_F_E10_13.ab1 1 NNNNNNNNNN NNNNNNNNAN NNGTGNNGNN GCTTTTTTGG AGGCCNNNNN NTTTGCAAAA 61 AGCTCCCGGG AGCNNGNNNN NNNNTTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC 121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA 181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG 241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG 301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT 361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC 421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG 481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA 541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GNTTACNAAT AAAGCAATAG 721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCNCTGCAT TCTAGTTGTG GTTTGTCCNA 781 ACTCNTCNAT GTATCTTATC ATGTCTGTAT ANNGTCNNNC TCTANCTAGA NCTTGGCGTA 841 ANCATGGNCA TAGCTGNTTC CTGNGTGAAA TTGNTATCCN NTCNCNATTC CNNNCNANAT 901 ACNANCNNGN NNNATAAAGN GNNANNNNNG GGNNGNNNNN NGNNTGNNCT NNCNNNNNNN 961 NANNNNNNNN NN //