Resource data sheet
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CSIV-TRE-RfA-CMV-KT

Backbone vector of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by CMV promoter.

Catalog number RDB12876
Resource name CSIV-TRE-RfA-CMV-KT
Clone info. Backbone vector of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by CMV promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-CMV-KT (Plasmid)
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Additional form)) Dr. Miyawaki's Approval
Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12876 CSIV-TRE-RfA-CMV-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12876z.seq from Depositor
Electronic file PDF RDB12876.pdf from Depositor

Featured content

References

original Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12876_A5Ekp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_N_rev RDB12876_A5Eka_2.seq
>12876_12876_A5Ek_Cm_N_rev_E04_13.ab1
    1 CGAGTCCTAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
   61 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGACGGATCC TAACTCAAAA
  121 TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGCGGCCG
  181 CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT
  241 CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TTTTGTACAA
  301 ACTTGTGATG CTAGCGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA TATAGGCCTC
  361 CCACCGTACA CGCCTACCTC GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA
  421 TACGTTCTCT ATCACTGATA GGGATAAACT CGACATACGT TCTCTATCAC TGATAGGGAG
  481 TAAACTCGAC ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC GTTCTCTATC
  541 ACTGATAGGG AGTAAACTCG ACATCGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA
  601 CGTTCTCTAT CACTGATAGG GAGTAAACTC GATTGAAGAC GAAAGGGCCG AATTCAAAGT
  661 GAATTTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA TTTTTGTAAT TTGTTTTTGT
  721 AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA TTCTTTCCCC TGCACTGTAC
  781 CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA CTGCCATTTG TAGATCCGTT
  841 CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT TCTTCTTCTA TTCCTTCGGG
  901 CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC GATAATGGTG AATATCCCTG
  961 CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT CTTAAACCCT ACCAAGCCTC
 1021 CTACTATCAT TATGAATAAT TTTATATACC ACAGCCATTG TATGTAACCA TTCCACAACT
 1081 GGCCATTATC TATCCATATT CTTGTTCATC CTTTCCTGCT GCTTGCGATC CGTCCACTTA
1141 CGGAGTGTAT TTAAGT
//
Primer: Cm_C_F RDB12876_A5Ekb_2.seq
>12876_12876_A5Ek_Cm_C_F_F04_16.ab1
    1 GGGGAGCGTG GATCGGCTTA CTAAAGCCAG ATAACAGTAT GCGTATTTGC GCGCTGATTT
   61 TTGCGGTATA AGAATATATA CTGATATGTA TACCCGAAGT ATGTCAAAAA GAGGTATGCT
  121 ATGAAGCAGC GTATTACAGT GACAGTTGAC AGCGACAGCT ATCAGTTGCT CAAGGCATAT
  181 ATGATGTCAA TATCTCCGGT CTGGTAAGCA CAACCATGCA GAATGAAGCC CGTCGTCTGC
  241 GTGCCGAACG CTGGAAAGCG GAAAATCAGG AAGGGATGGC TGAGGTCGCC CGGTTTATTG
  301 AAATGAACGG CTCTTTTGCT GACGAGAACA GGGGCTGGTG AAATGCAGTT TAAGGTTTAC
  361 ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG TACAGAGTGA TATTATTGAC
  421 ACGCCCGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC GTCTGCTGTC AGATAAAGTC
  481 TCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA GCTGGCGCAT GATGACCACC
  541 GATATGGCCA GTGTGCCGGT CTCCGTTATC GGGGAAGAAG TGGCTGATCT CAGCCACCGC
  601 GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCTCC
  661 CTTATACACA GCCAGTCTGC AGGTCGACCA TAGTGACTGG ATATGTTGTG TTTTACAGTA
  721 TTATGTAGTC TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA
  781 CGTTTCTCGT TCAGCTTTCT TGTACAAAGT GGTGATGCTA CCGGTCTCGA GGAGCTTGGC
  841 CCATTGCATA CGTTGTATCC ATATCATAAT ATGTACATTT ATATTGGCTC ATGTCCAACA
  901 TTACCGCCAT GTTGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACAGGGTCA
  961 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT
 1021 GGCTGACCGC CCAACGACCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCATAGTACG
 1081 CCAATACGGA CTTTTCATGA CGTCATGGGT GGAAGTATTT ACCGGTAACC TGCCCACTGG
1141 CAGTACATCA AGTGTATTCC ATATGGCTAG TTACGCCCCC CC
//
Primer: hKO_F RDB12876_A5Ekc_2.seq
>12876_12876_A5Ek_hKO_F_G04_19.ab1
    1 CATTACTCGG CAAGGCTGGT GAGGAGACCG AGGGCAACAT CACCGAGCTG GTGGAGGACG
   61 CCGTGGCCCA CTGCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC
  121 CCGGCCCTAT GTCTAGACTG GACAAGAGCA AAGTCATAAA CGGCGCTCTG GAATTACTCA
  181 ATGGAGTCGG TATCGAAGGC CTGACGACAA GGAAACTCGC TCAAAAGCTG GGAGTTGAGC
  241 AGCCTACCCT GTACTGGCAC GTGAAGAACA AGCGGGCCCT GCTCGATGCC CTGCCAATCG
  301 AGATGCTGGA CAGGCATCAT ACCCACTTCT GCCCCCTGGA AGGCGAGTCA TGGCAAGACT
  361 TTCTGCGGAA CAACGCCAAG TCATTCCGCT GTGCTCTCCT CTCACATCGC GACGGGGCTA
  421 AAGTGCATCT CGGCACCCGC CCAACAGAGA AACAGTACGA AACCCTGGAA AATCAGCTCG
  481 CGTTCCTGTG TCAGCAAGGC TTCTCCCTGG AGAACGCACT GTACGCTCTG TCCGCCGTGG
  541 GCCACTTTAC ACTGGGCTGC GTATTGGAGG AACAGGAGCA TCAAGTAGCA AAAGAGGAAA
  601 GAGAGACACC TACCACCGAT TCTATGCCCC CACTTCTGAG ACAAGCAATT GAGCTGTTCG
  661 ACCGGCAGGG AGCCGAACCT GCCTTCCTTT TCGGCCTGGA ACTAATCATA TGTGGCCTGG
  721 AGAAACAGCT AAAGTGCGAA AGCGGCGGGC CGGCCGACGC CCTTGACGAT TTTGACTTAG
  781 ACATGCTCCC AGCCGATGCC CTTGACGACT TTGACCTTGA TATGCTGCCT GCTGACGCTC
  841 TTGACGATTT TGACCTTGAC ATGCTCCCCG GGTAACTAAG TAAGGATCCT CTAGAGTTAA
  901 CATCGAGGGA TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG
  961 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT
 1021 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CTGTGTATAA ATCCTGATGC
 1081 TGTCTCTTTA TGAGAGTGTG GCCGTTGTCA GCCACGTGCC GTGGTGTGCA CTTGTGATTG
1141 CTGACGCACT CCACTGGATT GGGGGCATTG G
//
Primer: HIVgp_R1 RDB12876_A5Ekd_2.seq
>12876_12876_A5Ek_HIVgp_R1_E10_13.ab1
    1 CGACATCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGCTTGCTGT GCGGTGGTCT
  181 TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA
  241 TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG
  301 ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT
  361 CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT
  421 TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA
  481 TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG
  541 TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG
  601 CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT
  661 TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
  721 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
  781 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
  841 CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCCAC CGTACACGCC
  901 TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TTGTTACGAC ATTTTTGGAA AGTCCCGTTT
  961 GATTTTGGGT GCCCAAAACA AACTCCCATT GACGTCAATG GGGGTGGAGA CTTGAAATCC
 1021 CCCGTGAGTC AAACCGCTAT CCACGCCCAA TTGATGTACT GGCTGAAACG GCATCAACCA
1081 TTGGTTAATA AGCGGAATGG AT
//
Primer: BGH_rev RDB12876_A5Eke_2.seq
>12876_12876_A5Ek_BGH_rev_H04_22.ab1
    1 CGTTACGGGG GACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
   61 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
  121 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
  181 AACCAGAGAG ACCCAGTACA GTCCGGAATA ACTTCGTATA ATGTATGCTA TACGAAGTTA
  241 TGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT TCTTTTAAAA
  301 AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC TGAGGTGTGA
  361 CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA GCATTGGTAG
  421 CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG GTCTCGACGG TATCGATGCG
  481 GGGAGGCGGC CCAAAGGGAG ATCCGACTCG TCTGAGGGCG AAGGCGAAGA CGCGGAAGAG
  541 GCCGCAGAGC CGGCAGCAGG CCGCGGGAAG GAAGGTCCGC TGGATTGAGG GCCGAAGGGA
  601 CGTAGCAGAA GGACGTCCCG CGCAGAATCC AGGTGGCAAC ACAGGCGAGC AGCCATGGAA
  661 AGGACGTCAG CTTCCCCGAC AACACCACGG AATTGTCAGT GCCCAACAGC CGAGCCCCTG
  721 TCCAGCAGCG GGCAAGGCAG GCGGCGATGA GTTCCGCCGT GGCAATAGGG AGGGGGAAAG
  781 CGAAAGTCCC GGAAAGGAGC TGACAGGTGG TGGCAATGCC CCAACCAGTG GGGGTTGCGT
  841 CAGCAAACAC AGTGCACACC ACGCCACGTT GCCTGACAAC GGGCCACAAC TCCTCATAAA
  901 GAGACAGCAA CCAGGATTTA TACAAGGAGG AGAAAATGAA AGCCATACGG GAAGCAATAG
  961 CATGATACAA AGGCATTAAA GCAGCGTATC CACATAGCGT AAAAGGAGCA ACATAGTTTA
 1021 AGAATACCAG TCAATCTTTC ACAAATTTTG TAATCCAGAG GTTTGATTAT CGATAAGCTG
 1081 ATCCCCTCGA TGTTAACTTC TAGAGATCCT ACTAGTTACC CGGGGGAGCA ATGTCAGTCA
 1141 AAATCGTCAG ATCGTCAGCC AGGCCAGCCA TAATCCAGTC AGTCGTCAGG CCATCGGTCC
1201 TGTGGA
//
Primer: SV40pro_F RDB12876_A5Ekf_2.seq
>12876_12876_A5Ek_SV40pro_F_V2_F10_16.ab1
    1 GTTGCACCTT TTTTGGGAGG CCTAGGCTTT TGCAAAAGCT CCCGGGAGCT TGTATATCCA
   61 TTTTCGGATC TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT
  121 ATAATACGAC AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT
  181 CACCGCGCGC GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG
  241 GGACTTCGTG GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG
  301 CGCGGTCCAG GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT
  361 GGACGAGCTG TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG
  421 GCCGGCCATG ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC
  481 GGCCGGCAAC TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT
  541 CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG
  601 CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT
  661 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAAATAAAA
  721 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT
  781 GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
  841 GTGTGAAATT GTTATCCGCT CACAATTTCC ACACAACATA CGAGCCGGAA AGCATAAAAG
  901 TGTAAAAGCC TGGGGGTGCC CTAAATGAAG TGAAGCTAAC TTCACATTTA AATTTGCGTT
  961 GCGCTCACTG CCCGCTTTCC AAGTTCGGGG AACCTGGTCG TGCCAGCTGG CATTAAATGG
 1021 AAATCCGGCC CAAACGCGCC GGGGGAGGAG GCGTTTGCCG TAATTTGGGG CGGCTCATTC
1081 CGACTTCATT CGACTCAACT TGGAACTCCG CCGGGCGACC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123.
reference Umei, T.C., Single-Construct Polycistronic Doxycycline-Inducible Vectors Improve Direct Cardiac Reprogramming and Can Be Used to Identify the Critical Timing of Transgene Expression. Int. J. Mol. Sci. 18 (8). pii: E1805 (2017). PMID 28825623.
reference Chapnick, D.A., Leader cell positioning drives wound-directed collective migration in TGFβ-stimulated epithelial sheets. Mol. Biol. Cell, 25 (10): 1586-1593 (2014). PMID 24623725.
reference Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.
reference Tai, M.C., miR-342-3p regulates MYC transcriptional activity via direct repression of E2F1 in human lung cancer. Carcinogenesis 36 (12): 1464-1473 (2015). PMID 26483346.
reference Lu, J., Expression of N-Acetylglucosaminyltransferase III Suppresses α2,3-Sialylation, and Its Distinctive Functions in Cell Migration Are Attributed to α2,6-Sialylation Levels. J. Biol. Chem. 291 (11): 5708-5720 (2016). PMID 26801611.
user report Ito, N., Direct reprogramming of fibroblasts into skeletal muscle progenitor cells by transcription factors enriched in undifferentiated subpopulation of satellite cells. Sci. Rep. 7 (1): 8097 (2017). PMID 28808339.

2018.10.09

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