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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSIV-TRE-RfA-CMV-KT

Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by CMV promoter.

Catalog number RDB12876
Resource name CSIV-TRE-RfA-CMV-KT
Clone info. Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-CMV-KT (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence RDB12876zzt.seq
            Remarks, protocol and/or map (pdf) RDB12876.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Additional terms and conditions:
            RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            付加的提供条件:
            利用者は、hKO1蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            宮脇敦史博士による提供承諾書 [Word]
            備考 【追加提供依頼書】受入れ確認書(書式J)
            このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB12876 CSIV-TRE-RfA-CMV-KT DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12876_A4Klp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: KO-M_R
            Region: hKO1 5', CMV pro2
            Sequence file: RDB12876_A4Kla.seq check
            >12876_12876_B0BiS_KO-M_R1_A06_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNTGGNNGCT CCTCTCCNNG CTCNGGCCCT CGGGGAAGGC
               61 CTGCTTGAAG TAGTCGGGGN NCTCCTCGGG GTACTTGGTG AAGGGCCTGT GGCCGTAGCA
              121 GAAGGTGTGG CTCACCAGGT CGAAGCTGAA GGGCATGGGG CCGCCCTTGG CCATTGTCAC
              181 CCTCAGGGTC ATCTCCTGGT GGCCCTCGTA GGGCTTGCCG GTGCCCTCGC CCTCCACGGT
              241 GAACTCGTGG CCGTTCACGC TGCCGTCCAT GAAGTACTTC ATCTTCATCT CGGGCTTGAT
              301 CACGCTCACC ATGGTGGACC GGTGAATTCG GGGCCGCGGA GGCTGGATCG GTCCCGGTGT
              361 CTTCTATGGA GGTCAAAACA GCGTGGATGG CGTCTCCAGG CGATCTGACG GTTCACTAAA
              421 CGAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG
              481 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCNAAA CAAACTCCCA
              541 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA
              601 TTGATGTACT GCCNAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC
              661 TGCCNAGTAG GAAAGTCCCA TAANGTCATG TACTGGGCAT AATGNCAGGC GGGCCATTTA
              721 CCGTCATTGA CGTCAATANG GGGCGTACTN NGCATATGAT ACACTTGATG TACTGNNNAN
              781 NGGGCAGTTT ACCGNAAATA CTCCACCNNT NNNCNTCNNN GGNAAGNNCN NATNNNNNTT
              841 ACTATGGGNN NNTACNNCNN TNNNTNGANN NNNNNNGGCN GGGGNNNNNN GGCNNNCANC
            901 CNNNNNGGNC NNNNNNNNNN
            //
            Primer: hKO_F
            Region: T2A, rtTA-Ad
            Sequence file: RDB12876_A4Klb.seq check
            >12876_12876_A4Kl_2_hKO_F_A10_01.ab1
                1 NNNNNNNNNN NNNGCNNGNN NNGANNNCGA NGGCAACATC ACNNNNNNGG TGGAGGANGC
               61 CGTGGCCCAC TGNNNNNNCA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC
              121 CGGCCCTATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC GGCGCTCTGG AATTACTCAA
              181 TGGAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA
              241 GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA
              301 GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT
              361 TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA
              421 AGTGCATCTC GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC
              481 GTTCCTGTGT CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG
              541 CCACTTTACA CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG
              601 AGAGACACCT ACCACCGATT CTATGCCCCC ACTTCTGAGA CAAGCAATTG AGCTGTTCGA
              661 CCGGCAGGGA GCCGAACCTG CCTTCCTTTT CGGCCTGGAA CTAATCATAT GTGGCCTGGA
              721 GAAACAGCTA AAGTGCGAAA GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTANA
              781 CATGCTCCCA GCCGATGCCC TTGNCGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT
              841 TGACGANTTT GACCTTGACA TGCTCCCCGG GNAACTAAGT AANGNTCCTC TAGAGTTAAC
              901 ATCNAGGGNT CNAGCTTATC GATAATCAAC NNCNNGATTA CNAAANTNGN NAAANANTGN
            961 CNGGNANNNN NNNNTATGNT GNNNNNTTTN NNNNNNNNN
            //
            Primer: Cm_N_rev
            Region: attR1, tight TRE pro, cPPT_CTS
            Sequence file: RDB12876_A4Klc.seq check
            >12876_12876_A4Kl_2_Cm_N_rev_B10_04.ab1
                1 NNNNNNNNNN NNTNTTNNNG NNGCNNTTGG GNNNTATCAA CNNNNNNATA TCCNGTGATT
               61 TTTTTCTCCN NNNNNNNNTC CTTAGCTCCT GAAAATCTCG ACGGATCCTA ACTCAAAATC
              121 CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGCGGCCGCC
              181 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT
              241 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTT TTGTACAAAC
              301 TTGTGATGCT AGCGGCGATC TGACGGTTCA CTAAACGAGC TCTGCTTATA TAGGCCTCCC
              361 ACCGTACACG CCTACCTCGA CATACGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA
              421 CGTTCTCTAT CACTGATAGG GATAAACTCG ACATACGTTC TCTATCACTG ATAGGGAGTA
              481 AACTCGACAT ACGTTCTCTA TCACTGATAG GGAGTAAACT CGACATACGT TCTCTATCAC
              541 TGATAGGGAG TAAACTCGAC ATCGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG
              601 TTCTCTATCA CTGATAGGGA GTAAACTCGA TTGAAGACGA AAGGGCCGAA TTCAAAGTGA
              661 ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA
              721 TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC
              781 CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGANTACT GCCATTTGTA GATCCGTTCA
              841 CTAATCNAAT GGATCTNNCT CTGNCTCTCT CTNCNCCTTC NNCNNNTATT NCNNCNGNNC
              901 TGTCNGNNCC CNNNNGGNNN NGNANGNNGN NNTGAANCNA NNNNGGNNNN NNTNCNNNNN
            961 NNANTNNNNN NNNNN
            //
            Primer: CS-CMVen_upR
            Region: attR2, ccdB
            Sequence file: RDB12876_A4Kld.seq check
            >12876_12876_B0BiS_CS-CMVen_upR_B06_02_ABI08.ab1
                1 NNNNNNNNNN NNNANNGANN NGGNNNNNNN NTATGCAATG GGCCNAGCTC CTCGAGACCG
               61 GTAGCATCAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
              121 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA
              181 TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT
              241 CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC
              301 ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG
              361 CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA
              421 CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT
              481 ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATT
              541 TCACCAGCCC CTGTTCTCGT CAGCAAAAGA GCCGTTCATT TCAATAAACC GGGCGACCTC
              601 AGCCATCCCT TCCTGATTTT CCGCTTTCCA GCGTTCGGCA CGCAGACGAC GGGCTTCATT
              661 CTGCATGGTT GTGCTTACNN GACCGGANAT ATTGACATCA TATATGCCTT GANCAACTGA
              721 TAGCTGTCGC TGTCAACTGT CACTGTAATA CGCTGCTTCN TANCATACCT CTTTTTGACA
              781 TACTTCNGGT ATACNTATCA GTATATATTC NTATANCGCA AAAATCANCG CGCAAATACN
            841 CATACNGNNN TCTGGNTTTN ANNNNNNCNG NNNNNNCGCN NTNNNNNNNN NN
            //
            Primer: tTA-Ad R
            Region: T2A, hKO1 3'
            Sequence file: RDB12876_A4Kle.seq check
            >12876_12876_B0BiS_tTA-Ad_R_C06_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT NNNNTCTTGT CNNGTCTAGA CATAGGGCCG
               61 GGNTTCTCCT CCACGTCACC GCATGTTAGA AGACTTCCTC TGCCCTCGCA GTGGGCCACG
              121 GCGTCCTCCA CCAGCTCGGT GATGTTGCCC TCGGTCTTCC TCACCAGCCT GTGGCCGATG
              181 AAGTGGCTCT GGGGCATCTT CAGGATCTTC TTGGCGGCCT TGTAGGTGGT CTTGAACTGG
              241 CACTTGTGGT TGCCGCCGCC GGCCAGCTTC AGGTACATGG TCACGTCGCC CTTCAGCACG
              301 CCGTCGCAGG TGGTGATCTT CTCGGTGCTG GGCTCCCAGT CGCTGCTCTG GTTCTGCATC
              361 ACGGGGCCGT CGGCGGGGAA GTTCACGCCC ACGAACTTGC TCTTGTGCTC GAAGCAGTTG
              421 CCCCTCAGGC TGATGTGGGC GCTCACGGCG GCGAAGCCGC CGTCCTCGAA CTGGAGGCTC
              481 CTCTCCCAGC TCAGGCCCTC GGGGAAGGCC TGCTTGAAGT AGTCGGGGAT CTCCTCGGGG
              541 TACTTGGTGA AGGGCCTGTG GCCGTAGCAG AANGTGTGGC TCACCAGGTC GAAGCTGAAG
              601 GGCATGGGGC CGCCCTTGGC CATTGTCACC CTCAGGGTCA TCTCCTGNTG GCCCTCGTAN
              661 GGCTTGCCGG TGCCCTCGCC CTCCACGGTG AACTCGTGGC CGTTCACGCT GCCGTCCATG
              721 AAGTACTTCA TCTTCATCTC GGGCTTGATC ACGCTCACNA TGGTGGNNCG GTGAATTCNG
              781 GGCCGCGGNN GCTGGATCGG TCCCGGTGTC TTCTATGGAN GTCAAAACAG CGTGGATGGC
              841 GTCTCCNNGC GATCTGANGG TTCACTNAAN NGANCTNTGN TNATATAGAN CNNCNNCCGN
              901 ACNCGCNNNN CNNCNNNTTG NNNCNATGGG NNNGNANNNG NTNNNANNTT TNGGAANNTC
            961 CNNNNNNNTT NNNNNNNNNA ANNNNNNNNC NNNNTNNNNN NNNNN
            //
            Primer: HIVgp_R1
            Region: HIV-1 psi, 5' LTR
            Sequence file: RDB12876_A4Klf.seq check
            >12876_12876_A4Kl_2_HIVgp_R1_F10_16.ab1
                1 NNNNNNNNNT NNNNNNNNNC TTTTTTCTCT CTGCACCNCT CTNCNCTTTG CCTTGGTGGG
               61 TGCTACTNNN NNNNNNNNAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
              121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGCTTGCTGT GCGGTGGTCT
              181 TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA
              241 TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG
              301 ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT
              361 CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT
              421 TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA
              481 TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG
              541 TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG
              601 CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT
              661 TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGANGG ATCTCTAGTT
              721 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
              781 ANGCTTAAGC AGTGGGTTCN CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
              841 CCAGANAGAC NNAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCNAC CNTACACGCC
              901 NANCGCCCAT TTGCGTCAAT GGGGCGGANT NNNNACNANA TTTNNNAAAG TCCCNNNNAN
              961 TTNNNNNNNN AAACAAANTC CNNTTNNNNN NNNNGGGGGN NNNNNNTNNN NNANNNNNNN
            1021 NNN
            //
            Primer: BGH_rev2
            Region: BGH p(A), 3'LTR, loxP, PRE
            Sequence file: RDB12876_A4Klg.seq check
            >12876_12876_B0BiS_BGH_rev2_D06_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGNNGGNNN NNNACAGATG GCTGGCAACT AGAAGGCACA
               61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
              121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
              181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
              241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGAATAA CTTCGTATAA
              301 TGTATGCTAT ACGAAGTTAT GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT
              361 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT
              421 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG
              481 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG
              541 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGAGGGCGA
              601 AGGCGAAGAC GCGGAAGAGG CCGCAGAGCC GGCAGCAGGC CGCGGGAAGG AAGGTCCGCT
              661 GGATTGAGGG CCGAAGGGAC GTAGCAGAAG GACGTCCCGC GCAGAATCCA GGTGGCAACA
              721 CAGGCGAGCA GCCATGGAAA GGACGTCAGC TTCCCCGACA ACACCACGGA ATTGTCAGTG
              781 CCCAACAGCC GANCCCCTGT CCAGCAGCGG GCAAGGCAGG CGGCGANGAN TTCCGCCGNG
              841 GCAATANGGA NGGGGGAAAG CGAAAGTCCC GGAAAGGANC NNANAGGNGG NGGCNNNGCC
              901 CCNANCNNNG GGGGGTTGCG NCAGCAAACN CNNNGCNNAN NNNNNCNNNN NTNNNCNNGA
            961 NNNNGGNNNN NNNNNN
            //
            Primer: SV40pro_F
            Region: Zeo, SV40 p(A)
            Sequence file: RDB12876_A4Klh.seq check
            >12876_12876_A4Kl_2_SV40pro_F_E10_13.ab1
                1 NNNNNNNNNN NNNNNNNNAN NNGTGNNGNN GCTTTTTTGG AGGCCNNNNN NTTTGCAAAA
               61 AGCTCCCGGG AGCNNGNNNN NNNNTTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
              121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
              181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
              241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
              301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
              361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
              421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
              481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
              541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
              601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
              661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GNTTACNAAT AAAGCAATAG
              721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCNCTGCAT TCTAGTTGTG GTTTGTCCNA
              781 ACTCNTCNAT GTATCTTATC ATGTCTGTAT ANNGTCNNNC TCTANCTAGA NCTTGGCGTA
              841 ANCATGGNCA TAGCTGNTTC CTGNGTGAAA TTGNTATCCN NTCNCNATTC CNNNCNANAT
              901 ACNANCNNGN NNNATAAAGN GNNANNNNNG GGNNGNNNNN NGNNTGNNCT NNCNNNNNNN
            961 NANNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123.
            reference Umei, T.C., Single-Construct Polycistronic Doxycycline-Inducible Vectors Improve Direct Cardiac Reprogramming and Can Be Used to Identify the Critical Timing of Transgene Expression. Int. J. Mol. Sci. 18 (8). pii: E1805 (2017). PMID 28825623.
            reference Chapnick, D.A., Leader cell positioning drives wound-directed collective migration in TGFβ-stimulated epithelial sheets. Mol. Biol. Cell, 25 (10): 1586-1593 (2014). PMID 24623725.
            reference Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.
            reference Tai, M.C., miR-342-3p regulates MYC transcriptional activity via direct repression of E2F1 in human lung cancer. Carcinogenesis 36 (12): 1464-1473 (2015). PMID 26483346.
            reference Lu, J., Expression of N-Acetylglucosaminyltransferase III Suppresses α2,3-Sialylation, and Its Distinctive Functions in Cell Migration Are Attributed to α2,6-Sialylation Levels. J. Biol. Chem. 291 (11): 5708-5720 (2016). PMID 26801611.
            user_report Orimoto, A., Establishment of human airway epithelial cells with doxycycline-inducible cell growth and fluorescence reporters. Cytotechnology 73 (4): 555-569 (2021). PMID 34349346.
            user_report Ito, N., Direct reprogramming of fibroblasts into skeletal muscle progenitor cells by transcription factors enriched in undifferentiated subpopulation of satellite cells. Sci. Rep. 7 (1): 8097 (2017). PMID 28808339.

            2022.10.11

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