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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSIV-TRE-GFP-CMV-KT

Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.

Catalog number RDB12875
Resource name CSIV-TRE-GFP-CMV-KT
Clone info. Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-CMV-KT ()
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering Please visit Ordering instruction.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Schedule A (GFP-A) [Link]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12875 CSIV-TRE-GFP-CMV-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB12875z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB12875.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17885_B0Atp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N
Region: attB1,tight TRE Pro,cPPT_CTS
Sequence file: RDB17885_B0Ata.seq check
>12875_17885_B0At_1_EGFP-N_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNN NNNNNGGTGG AGCCTGCTTT TTTGTACAAA
   61 CTTGTGATGC TAGCGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTTAT ATAGGCCTCC
  121 CACCGTACAC GCCTACCTCG ACATACGTTC TCTATCACTG ATAGGGAGTA AACTCGACAT
  181 ACGTTCTCTA TCACTGATAG GGATAAACTC GACATACGTT CTCTATCACT GATAGGGAGT
  241 AAACTCGACA TACGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG TTCTCTATCA
  301 CTGATAGGGA GTAAACTCGA CATCGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC
  361 GTTCTCTATC ACTGATAGGG AGTAAACTCG ATTGAAGACG AAAGGGCCGA ATTCAAAGTG
  421 AATTTCTGCT GTCCCTGTAA TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA
  481 ATTCTTTAGT TTGTATGTCT GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC
  541 CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC TGCCATTTGT AGATCCGTTC
  601 ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT CTTCTTCTAT TCCTTCGGGC
  661 CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG ATAATGGTGA ATATCCCTGC
  721 CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC TTAAACCTAC CAAGCCTCCT
  781 ACTATCATTA TGAATAATTT TATATACNAC AGCCAATTTG TTATGTTAAA CCNATTCCNC
  841 AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT TCTTTTCTTG NTGNNTTTGC
  901 GATTCNTCAA TTAAGGAGTG NATTAANCTT GTGNNATTGT TAATTTCTCN GNNCNNCTNN
  961 NNNCANGNCG NGNGATNNCN AATCTGNTNN ANANATTTAT TANNCCNNCN ANNATNNNNN
1021 NNNNN
//
Primer: FP-C_F1
Region: EGFP 3',CMV en.2
Sequence file: RDB17885_B0Atb.seq check
>12875_17885_B0JkF_FP-C_F1_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCGCGNTCNC ATGGTCCTGC TNNNNNNCGT GACCGCCGCC
   61 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGAC TCAGATCCTA CTAGTAGGAT
  121 CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTGGTG ATGCTACCGG TCTCGAGGAG
  181 CTTGGCCCAT TGCATACGTT GTATCCATAT CATAATATGT ACATTTATAT TGGCTCATGT
  241 CCAACATTAC CGCCATGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG
  301 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
  361 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
  421 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
  481 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
  541 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT
  601 TGGCAGTACA TCTACGTATT AGTCNTCGCT ATTACCATGG TGANGCGGNT TTGGNAGTAC
  661 ATCNATGGGG CGTGGANAGC GGTTTGACTC NCGGGGNNTT CCNNNNCTCC NCCCCNTTGN
721 CNTCNNTNGG ANTTNGNTTN NGNNNNNAAN NNNNNNGGNN NNTTNNNNAA NNN
//
Primer: PRE_rev
Region: PRE,rt TA Advanced
Sequence file: RDB17885_B0Atc.seq check
>12875_17885_B0At_1_PRE_rev_G11_20_ABI24.ab1
    1 NNNNNNNNNN NNNNCGNNNA NNGAGCANCA TAGTTAAGAA TACCAGTCAA TCTTTCNCAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG
  121 ATCCTTACTT AGTTACCCGG GGAGCATGTC AAGGTCAAAA TCGTCAAGAG CGTCAGCAGG
  181 CAGCATATCA AGGTCAAAGT CGTCAAGGGC ATCGGCTGGG AGCATGTCTA AGTCAAAATC
  241 GTCAAGGGCG TCGGCCGGCC CGCCGCTTTC GCACTTTAGC TGTTTCTCCA GGCCACATAT
  301 GATTAGTTCC AGGCCGAAAA GGAAGGCAGG TTCGGCTCCC TGCCGGTCGA ACAGCTCAAT
  361 TGCTTGTCTC AGAAGTGGGG GCATAGAATC GGTGGTAGGT GTCTCTCTTT CCTCTTTTGC
  421 TACTTGATGC TCCTGTTCCT CCAATACGCA GCCCAGTGTA AAGTGGCCCA CGGCGGACAG
  481 AGCGTACAGT GCGTTCTCCA GGGAGAAGCC TTGCTGACAC AGGAACGCGA GCTGATTTTC
  541 CAGGGTTTCG TACTGTTTCT CTGTTGGGCG GGTGCCGAGA TGCACTTTAG CCCCGTCGCG
  601 ATGTGAGAGG AGAGCACAGC GGAATGACTT GGCGTTGTTC CGCAGAAAGT CTTGCCATGA
  661 CTCGCCTTCC AGGGGGCAGA AGTGGGTATG ATGCCTGTCC AGCATCTCGA TTGGCAGGGC
  721 ATCGAGCAGG GCCCGCTTGT TCTTCACGTG CCAGTACAGG GTAGGCTGCT CAACTCCCAG
  781 CTTTTGAGCG AGTTTCCTTG TCGTCAGGCC TTCGATACCG ACTCCATTGA GTAATTCCAG
  841 ANCGCCGTTT ATGANTTTGC TCTTGTCCNG TCTAGACATA NGGCCGGGAT TCTCCTCCNC
  901 GTCACCGCAN GTTAGAAGAN NTCCNCTGNC CNCGCANTGG GCCACGGNGT CCNNCACCAN
  961 CNCGGNGANG TTGNCNNCGG NCTTNNNNNN NANNNTGNNG NNNNANGNNN TNNCNNTGGG
1021 GNNTCNTNNN NNNNNNN
//
Primer: tTA-Ad R
Region: T2A,hKO
Sequence file: RDB17885_B0Atd.seq check
>12875_17885_B0At_1_tTA-AdR_F11_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNNN GANTTTGCTC TTGTCNNGTC TAGACNTAGG GCCGGGNTTC
   61 TCCTCCACGT CACCGCATGT TAGAAGACTT CCTCTGCCCT CGCAGTGGGC CACGGCGTCC
  121 TCCACCAGCT CGGTGATGTT GCCCTCGGTC TTCCTCACCA GCCTGTGGCC GATGAAGTGG
  181 CTCTGGGGCA TCTTCAGGAT CTTCTTGGCG GCCTTGTAGG TGGTCTTGAA CTGGCACTTG
  241 TGGTTGCCGC CGCCGGCCAG CTTCAGGTAC ATGGTCACGT CGCCCTTCAG CACGCCGTCG
  301 CAGGTGGTGA TCTTCTCGGT GCTGGGCTCC CAGTCGCTGC TCTGGTTCTG CATCACGGGG
  361 CCGTCGGCGG GGAAGTTCAC GCCCACGAAC TTGCTCTTGT GCTCGAAGCA GTTGCCCCTC
  421 AGGCTGATGT GGGCGCTCAC GGCGGCGAAG CCGCCGTCCT CGAACTGGAG GCTCCTCTCC
  481 CAGCTCAGGC CCTCGGGGAA GGCCTGCTTG AAGTAGTCGG GGATCTCCTC GGGGTACTTG
  541 GTGAAGGGCC TGTGGCCGTA GCAGAAGGTG TGGCTCACCA GGTCGAAGCT GAAGGGCATG
  601 GGGCCGCCCT TGGCCATTGT CACCCTCAGG GTCATCTCCT GGTGGCCCTC GTAGGGCTTG
  661 CCGGTGCCCT CGCCCTCCAC GGTGAACTCG TGGCCGTTCA CGCTGCCGTC CATGAAGTAC
  721 TTCATCTTCA TCTCGGGCTT GATCACGCTC ACCATGGTGG ANCGGTGAAT TCGGGGCCGC
  781 GGANGCTGGA TCGGTCCCGG TGTCTTCTAT GGANGTCAAA ACAGCGTGGA TGGCGTCTCC
  841 AGGCGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA GACCTCCNAC CGTACACGCC
  901 TACNGCCCAT TTGCGTCNAT GGGGGCGGAN TTGTTACNAN NTTTNNGAAA GTCCCGTTGA
  961 TTTTGGTGNC NAAACAAACT CCNNTTGNNG TNNNNGGGGG NNNNNNNTNN NNANTCCNNN
1021 NNANNCNAAN CNGNNNTCNN NNNNNNNN
//
Primer: HIV-B_R
Region: HIV-1 psi,5' LTR,CMV en.1,CMV pro1
Sequence file: RDB17885_B0Ate.seq check
>12875_17885_B0At_1_HIV-B_R_C11_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNANNTTTT GGNGTACTCN CCAGTCGCCG CCCCTCGCCT
   61 CTTGCCGTGC GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT
  121 TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG
  181 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
  241 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
  301 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG
  361 ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT
  421 TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC AATGGGGTGG
  481 AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA CTGCCAAAAC
  541 CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC
  601 CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA
  661 GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA
  721 TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC
  781 ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA
  841 AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA ATGANCCCGN AATTGATTAC
  901 TATTAATAAC TAGTCAATAA TCNATGTCNA CGCGTATATC TGGNCCNNAN ATCGCGAANC
  961 AGCGCAAAAC GCCNAACCNT AANCAGANTC TTCNTGNNNN TGNCNGNCNN NNNTNNNNNN
1021 NNTNNNANCN NNAATTTTGN NCNCNNNNNN NNNN
//
Primer: attB2-R1
Region: EGFP mid,Tight TRE pro
Sequence file: RDB17885_B0Atf.seq check
>12875_17885_B0At_1_attB2-R1_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTGA NTCCGGANTT GTACNGCTCG TCCNTGCCGA
   61 GAGTGATCCC GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG
  121 GGTCTTTGCT CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC
  181 CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA
  241 TGTTGTGGCG GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT
  301 AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT
  361 TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT
  421 CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC
  481 CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA
  541 AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC
  601 CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC
  661 CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA
  721 CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTGGA GCCTGCTTTT TTGTACAAAC
  781 TTGTGATGCT AGCGGCGATC TGACGGTTCA CTAAACGAGC TCTGCTTATA TAGGCCTCCC
  841 ACCGTACACG CCTACCTCGA CATACGTTCT CTATCACTGA TANGGAGTAA ACTCGACATA
  901 CGTTCTCTAT CACTGATAGG GANAAACTCG ACNTACGTTC TCTATCNNNG ATANGGAGTA
  961 AANTCGACNT NCGTTCTCTA TCANNNATAG GGNNTAAACT NNNNNTACNT TCNNNANNAC
1021 NNNTANGGNN TAANNNNNNN NTNNNNNNNN ATCNNTNNNN NGNNNNNANN NNNNNNNN
//
Primer: BGH_rev2
Region: bGHpA,3' LTR,lox P,PRE
Sequence file: RDB17885_B0Atg.seq check
>12875_17885_B0At_1_BGH_rev2_H11_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG CNNACNACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
  121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
  181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
  241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAA TAACTTCGTA
  301 TAATGTATGC TATACGAAGT TATGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC
  361 AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT
  421 CTTAAAGGTA CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC
  481 CAGGCACAAT CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT
  541 AGGTCTCGAC GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG
  601 CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC
  661 GCTGGATTGA GGGCCGAAGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA
  721 ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACAACACCAC GGAATTGTCA
  781 GTGCCCAACA GCCGAGCCCC TGTCCAGCAG CGGGCAAGGC AGGCGGCGAT GANTTCCGCC
  841 GTGGCAATAG GGANGGGGAA AGCGAAAGTC CNGGAAAGGA NCTGANAGGN GGTGGNAATG
  901 NCCCAACCNG NGGGGGTTGC GTCAGCAAAC ACAGNGCACA NCACGCNNCG TTGNCNGANN
  961 ACGGGCCNCA NNTCCTCATA AAGANNNAGC AANCANNNNT TNTANNNNNA NNANAAAATG
1021 AAAGCNNTAN NGGNNNNNNT ANCNNNNNNN NANNNNNTTN ANNNNNNNNN N
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,Zeo
Sequence file: RDB17885_B0Ath.seq check
>12875_17885_B0At_1_SV40pro_ori_F_A12_03_ABI24.ab1
    1 NNNNCNNNNN NNNNGNNNCC TCTGCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
  121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA
  181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA
  241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA
  301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA
  361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA
  421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC
  481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG
  541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC
  601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT
  661 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA
  721 TAATGNTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT
  781 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC
  841 GANCTCTAGC TAGAGCTTGG CGTANTCATG GTCNTAGCTG TTTCCTGTGT GAAATTGTTA
  901 TCCGCTCACA ATTCCNCACA ACATACGANC NNGAAGCATA AAGTGNAAAG NCTGGGGNGN
  961 CTAATGANTG AGCTANNNCN CATTNNTTGC GTNGCGNNNA CNGNCNNCTT TNNNNNNNGN
1021 NNANNNNNNN NNGNNNGCNN NNNTNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.12.24

GNP_filter3_RDBDEP_html_201222.pl