Resource data sheet
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CSIV-TRE-GFP-CMV-KT

Lentivirus vector construct of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.

Catalog number RDB12875
Resource name CSIV-TRE-GFP-CMV-KT
Clone info. Lentivirus vector construct of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-CMV-KT ()
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12875 CSIV-TRE-GFP-CMV-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12875z.seq from Depositor
Electronic file PDF RDB12875.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12875_A5Ek.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N RDB12875_A5Eka.seq
>12875_12875_A5Ek_EGFP-N_A04_01.ab1
    1 GAACCCCGGG GGGACAGCTC CTCGCCCTTG CTCACCATGG TGGAGCCTGC TTTTTTGTAC
   61 AAACTTGTGA TGCTAGCGGC GATCTGACGG TTCACTAAAC GAGCTCTGCT TATATAGGCC
  121 TCCCACCGTA CACGCCTACC TCGACATACG TTCTCTATCA CTGATAGGGA GTAAACTCGA
  181 CATACGTTCT CTATCACTGA TAGGGATAAA CTCGACATAC GTTCTCTATC ACTGATAGGG
  241 AGTAAACTCG ACATACGTTC TCTATCACTG ATAGGGAGTA AACTCGACAT ACGTTCTCTA
  301 TCACTGATAG GGAGTAAACT CGACATCGTT CTCTATCACT GATAGGGAGT AAACTCGACA
  361 TACGTTCTCT ATCACTGATA GGGAGTAAAC TCGATTGAAG ACGAAAGGGC CGAATTCAAA
  421 GTGAATTTCT GCTGTCCCTG TAATAAACCC GAAAATTTTG AATTTTTGTA ATTTGTTTTT
  481 GTAATTCTTT AGTTTGTATG TCTGTTGCTA TTATGTCTAC TATTCTTTCC CCTGCACTGT
  541 ACCCCCCAAT CCCCCCTTTT CTTTTAAAAT TGTGGATGAA TACTGCCATT TGTAGATCCG
  601 TTCACTAATC GAATGGATCT GTCTCTGTCT CTCTCTCCAC CTTCTTCTTC TATTCCTTCG
  661 GGCCTGTCGG GTCCCCTCGG GGTTGGGAGG TGGGTCTGAA ACGATAATGG TGAATATCCC
  721 TGCCTAACTC TATTCACTAT AGAAAGTACA GCAAAAACTA TTCTTAAACC TACCAAGCCT
  781 CCTACTATCA TTATGAATAA TTTTATATAC CACAGCCAAT TTGTTATGTT AAACCAATTC
  841 CACAAACTTG CCCATTTATC TAATTCCAAT AATTCTTGTT CATTCTTTTC TTGCTGGTTT
  901 TGCGATTCTT CAATTAAGGA GTGTATTAAG CTTGTGTAAT TGTTAATTTC TCTGTCCCAC
  961 TCCATCCAGG TCGTGTGATT CCAAATCTGT TCCAGAGATT TATTACTCCC AACTAGCATT
 1021 TCCAAGGCAC AGCAGTGGGT GCAAATGAGT TTTCCAGAGC ACCCCAATCC CCAGGAAGCT
 1081 GGTGATCCTT TAGTATCTTC CACAGCAGGA TTCCTTGCTG AAGCCTGCTG GATGGCCCCA
1141 GACTGGTGGA AGTTGGCAC
//
Primer: EGFP-C RDB12875_A5Ekb.seq
>12875_12875_A5Ek_EGFP-C_B04_04.ab1
    1 ACGGTACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT CAGATCCTAC TAGTAGGATC
   61 TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTGA TGCTACCGGT CTCGAGGAGC
  121 TTGGCCCATT GCATACGTTG TATCCATATC ATAATATGTA CATTTATATT GGCTCATGTC
  181 CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
  241 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
  301 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
  361 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
  421 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
  481 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
  541 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA
  601 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG
  661 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT
  721 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
  781 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA
  841 GAAGACACCG GGACCGATCC AGCCTCCGCG GCCCCGAATT CACCGGTCCA CCATGGTGAG
  901 CGTGATCAAG CCCGAGATGA AGATGAAGTA CTTCATGGGA CGGCAGCGTG AACGGGCCAC
  961 GAGTTCACCG TGGAGGGCGA GGGCACCGGG CAAGCCCTAC GAGGGCCACC AGGAGATGAC
 1021 CCTGAGGGTG ACATGGCCAG GCGCCCCATG CCTCCAGCCT CGACTGTTGA GCACACTTCT
 1081 GCTACGCACA GCCCTCACAG TAACCCGAGG AGATCCCGAC TACCTCAGCA GCATTCCGAA
1141 GCTGATCTGC TGGGAGCTCC AAGCTTTCA
//
Primer: hKO_F RDB12875_A5Ekc.seq
>12875_12875_A5Ek_hKO_F_C04_07.ab1
    1 CCTATCGGGC AAGGCTGGTG AGGAGACCGA GGGCAACATC ACCGAGCTGG TGGAGGACGC
   61 CGTGGCCCAC TGCGAGGGCA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC
  121 CGGCCCTATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC GGCGCTCTGG AATTACTCAA
  181 TGGAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA
  241 GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA
  301 GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT
  361 TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA
  421 AGTGCATCTC GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC
  481 GTTCCTGTGT CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG
  541 CCACTTTACA CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG
  601 AGAGACACCT ACCACCGATT CTATGCCCCC ACTTCTGAGA CAAGCAATTG AGCTGTTCGA
  661 CCGGCAGGGA GCCGAACCTG CCTTCCTTTT CGGCCTGGAA CTAATCATAT GTGGCCTGGA
  721 GAAACAGCTA AAGTGCGAAA GCGGCGGGCC GGCCGACGCC CTTGACGATT TTGACTTAGA
  781 CATGCTCCCA GCCGATGCCC TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT
  841 TGACGATTTT GACCTTGACA TGCTCCCCGG GTAACTAAGT AAGGATCCTC TAGAGTTAAC
  901 ATCGAGGGAT CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA
  961 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAAATGCCT
 1021 TTGTATCATG CTATTGCTTC CCGTATGGGC TTTCATTTTT CTCTGTGTAT AAATCCTGCT
 1081 GGCTGTCTCT TTTATGAGAG TGTGTCGTGT CAAGCCACGT GCGTATGTGC ACTGTGTTGC
1141 CTGAACGCAC CCCCCACCTT GGAAG
//
Primer: BGH_rev RDB12875_A5Ekd.seq
>12875_12875_A5Ek_BGH_rev_D04_10.ab1
    1 CTTCGGGACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC
   61 CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG
  121 GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC
  181 AGAGAGACCC AGTACAGTCC GGAATAACTT CGTATAATGT ATGCTATACG AAGTTATGAT
  241 ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC CCCTTTTCTT TTAAAAAGTG
  301 GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA GGTACCTGAG GTGTGACTGG
  361 AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA CAATCAGCAT TGGTAGCTGC
  421 TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT CGACGGTATC GATGCGGGGA
  481 GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG CGAAGACGCG GAAGAGGCCG
  541 CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA TTGAGGGCCG AAGGGACGTA
  601 GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG GCGAGCAGCC ATGGAAAGGA
  661 CGTCAGCTTC CCCGACAACA CCACGGAATT GTCAGTGCCC AACAGCCGAG CCCCTGTCCA
  721 GCAGCGGGCA AGGCAGGCGG CGATGAGTTC CGCCGTGGCA ATAGGGAGGG GGAAAGCGAA
  781 AGTCCCGGAA AGGAGCTGAC AGGTGGTGGC AATGCCCCAA CCAGTGGGGG GTTGCGTCAG
  841 CAAACACAGT GCACACCACG CCACGTTGCC TGACAACGGG CCACAACTCC TCATAAAGAG
  901 ACAGCAACCA GGATTTATAC AAGGAGGAGA AAATGAAAGC CATACGGGAA GCAATAGCAT
  961 GATACAAAGG CATTAAAGCA GCGTATCCAC ATAGCGTAAA AGGAGCAACA TAGTTAAGAA
 1021 TACCAGTCAA TCTTTCACAA ATTTTGTAAT CCAGAGGTTG ATTATCGATA GCTGATCCCT
 1081 CGATGTAACT CTAGAGATCG TACTAGTACC CGGGAGCCAT GTCAGTTCAA AATCGTCAGA
1141 GCGTCAGCAG GCAAGCAATC AAGGTCAAAG TTCGGTTCCA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.09

GNP_filter3_RDBDEP_html_180823.pl