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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSIV-TRE-GFP-CMV-KT

Lentivirus vector construct of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.

Catalog number RDB12875
Resource name CSIV-TRE-GFP-CMV-KT
Clone info. Lentivirus vector construct of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-CMV-KT ()
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering Please visit Ordering instruction. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12875 CSIV-TRE-GFP-CMV-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB12875z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB12875.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12875_A5D2p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N
Sequence file: RDB12875_A5D2a.seq check
>12875_12875_A5D2_2_EGFP-N_C07_07.ab1
    1 GACCGGGCGA CGCTCCTCGC CCTTGCTCAC CATGGTGGAG CCTGCTTTTT TGTACAAACT
   61 TGTGATGCTA GCGGCGATCT GACGGTTCAC TAAACGAGCT CTGCTTATAT AGGCCTCCCA
  121 CCGTACACGC CTACCTCGAC ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC
  181 GTTCTCTATC ACTGATAGGG ATAAACTCGA CATACGTTCT CTATCACTGA TAGGGAGTAA
  241 ACTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC GACATACGTT CTCTATCACT
  301 GATAGGGAGT AAACTCGACA TCGTTCTCTA TCACTGATAG GGAGTAAACT CGACATACGT
  361 TCTCTATCAC TGATAGGGAG TAAACTCGAT TGAAGACGAA AGGGCCGAAT TCAAAGTGAA
  421 TTTCTGCTGT CCCTGTAATA AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT
  481 TCTTTAGTTT GTATGTCTGT TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC
  541 CCAATCCCCC CTTTTCTTTT AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC
  601 TAATCGAATG GATCTGTCTC TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT
  661 GTCGGGTCCC CTCGGGGTTG GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT
  721 AACTCTATTC ACTATAGAAA GTACAGCAAA AACTATTCTT AAACCTACCA AGCCTCCTAC
  781 TATCATTATG AATAATTTTA TATACCACAG CCAATTTGTT ATGTTAAACC AATTCCACAA
  841 ACTTGCCCAT TTATCTAATT CCAATAATTC TTGTTCATTC TTTTCTTGCT GGTTTTGCGA
  901 TTCTTCAATT AAGGAGTGTA TTAAGCTTGT GTAATTGTTA ATTTCTCTGT CCCACTCCAT
  961 CCAGGTCGTG TGATTCCCAA TCTGTTCCAG AGATTTTATT ACTCCAACTA GCATTCCAGG
 1021 CACAGCAGTG GTGCAAATGA GTTTTCAGAG CACCCCAATT CCCCAGAGCT GTTGGATTCA
 1081 TTAGTATCTT TCACAGTCAG GATCTGCCTG GAGCTGCTGA TGCCCAGACT GGTGAGCTTG
1141 CCAAACAGGA AT
//
Primer: EGFP-C
Sequence file: RDB12875_A5D2b.seq check
>12875_12875_A5D2_2_EGFP-C_D07_10.ab1
    1 CAGGGATTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT CAGATCCTAC TAGTAGGATC
   61 TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTGA TGCTACCGGT CTCGAGGAGC
  121 TTGGCCCATT GCATACGTTG TATCCATATC ATAATATGTA CATTTATATT GGCTCATGTC
  181 CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
  241 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
  301 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
  361 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
  421 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
  481 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
  541 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA
  601 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG
  661 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT
  721 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
  781 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA
  841 GAAGACACCG GGACCGATCC AGCCTCCGCG GCCCCGAATT CACCGGTCCA CCATGGTGAG
  901 CGTGATCAAG CCCGAGATGA AGATGAAGTA CTTCATGGAC GGCAGCGTGA ACGGGCCACG
  961 AGTTCACCGT GAGGGCGAGG GCACCGGGCA AGCCCTACGA GGGCCACCAG GAGATGACCC
 1021 TGAGGGTGAC ATGACCAGGC GCCCCATGCC TCAGCTCGAC TGTTGAGCCA CACCTTCTGC
 1081 TACGCACAGT CCGTCACAGT ACCGAGGAAT CCGACTACCC AGCAGGCTTC CCGAGGACTG
1141 GAAGCTTGGA AG
//
Primer: hKO_F
Sequence file: RDB12875_A5D2c.seq check
>12875_12875_A5D2_2_hKO_F_E07_13.ab1
    1 CGTAACGGCA AGGCTGGTGA GGAGACCGAG GGCAACATCA CCGAGCTGGT GGAGGACGCC
   61 GTGGCCCACT GCGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTGGA GGAGAATCCC
  121 GGCCCTATGT CTAGACTGGA CAAGAGCAAA GTCATAAACG GCGCTCTGGA ATTACTCAAT
  181 GGAGTCGGTA TCGAAGGCCT GACGACAAGG AAACTCGCTC AAAAGCTGGG AGTTGAGCAG
  241 CCTACCCTGT ACTGGCACGT GAAGAACAAG CGGGCCCTGC TCGATGCCCT GCCAATCGAG
  301 ATGCTGGACA GGCATCATAC CCACTTCTGC CCCCTGGAAG GCGAGTCATG GCAAGACTTT
  361 CTGCGGAACA ACGCCAAGTC ATTCCGCTGT GCTCTCCTCT CACATCGCGA CGGGGCTAAA
  421 GTGCATCTCG GCACCCGCCC AACAGAGAAA CAGTACGAAA CCCTGGAAAA TCAGCTCGCG
  481 TTCCTGTGTC AGCAAGGCTT CTCCCTGGAG AACGCACTGT ACGCTCTGTC CGCCGTGGGC
  541 CACTTTACAC TGGGCTGCGT ATTGGAGGAA CAGGAGCATC AAGTAGCAAA AGAGGAAAGA
  601 GAGACACCTA CCACCGATTC TATGCCCCCA CTTCTGAGAC AAGCAATTGA GCTGTTCGAC
  661 CGGCAGGGAG CCGAACCTGC CTTCCTTTTC GGCCTGGAAC TAATCATATG TGGCCTGGAG
  721 AAACAGCTAA AGTGCGAAAG CGGCGGGCCG GCCGACGCCC TTGACGATTT TGACTTAGAC
  781 ATGCTCCCAG CCGATGCCCT TGACGACTTT GACCTTGATA TGCTGCCTGC TGACGCTCTT
  841 GACGATTTTG ACCTTGACAT GCTCCCCGGG TAACTAAGTA AAGGATCCTC TAGAGTTAAC
  901 ATCGAGGGAT CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA
  961 CTGGTATTCT TAACTATGTT GCTCTTTTTA CGCTATGTGG ATACGCTGCT TAATGCTTTG
 1021 TATCATGCTA TGCTCCCGTA TGGGCTTCAT TTTCTCCTCC TTGTATAATC CTGGATGCTG
 1081 TTCTCTTTTA TGAGGAGTTG TCGTCGATGT CAAGCACTGC CTGGGTGTGT CACTGGTGGT
1141 TGGTCTGAGA AGCACAAAAA C
//
Primer: HIVgp_r1
Sequence file: RDB12875_A5D2d.seq check
>12875_12875_A5D2_2_HIVgp_R1_F07_16.ab1
    1 CAGCTCTATT CCACTGCTCT TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT
   61 GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG
  121 TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GCTTGCTGTG CGGTGGTCTT
  181 ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT
  241 CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA
  301 TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC
  361 TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT
  421 TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT
  481 TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT
  541 CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC
  601 AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT
  661 CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA
  721 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA
  781 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC
  841 CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG ACCTCCCACC GTACACGCCT
  901 ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCGTTGATTT
  961 TGGTGCCAAA ACAAACTCCC ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG
 1021 TCAACGCTAT CACGCCCATT GATGTACTGG CAAACGCATC ACATGGTATA GCGATGAACT
 1081 ACTACGGTAG ATGTACTGCT AGTAGAAGTT CCCATTAGTC ATGTTACCTG GGCCATAATT
1141 GCTAGGCGGG CCCAATTTAA CCGGCTCA
//
Primer: BGH_rev
Sequence file: RDB12875_A5D2e.seq check
>12875_12875_A5D2_2_BGHrev_G07_19.ab1
    1 GGTCGGACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC TCTAGTTACC
   61 AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG
  121 CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA
  181 GAGAGACCCA GTACAGTCCG GAATAACTTC GTATAATGTA TGCTATACGA AGTTATGATA
  241 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG
  301 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA
  361 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT
  421 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAG
  481 GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC GAAGACGCGG AAGAGGCCGC
  541 AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGAT TGAGGGCCGA AGGGACGTAG
  601 CAGAAGGACG TCCCGCGCAG AATCCAGGTG GCAACACAGG CGAGCAGCCA TGGAAAGGAC
  661 GTCAGCTTCC CCGACAACAC CACGGAATTG TCAGTGCCCA ACAGCCGAGC CCCTGTCCAG
  721 CAGCGGGCAA GGCAGGCGGC GATGAGTTCC GCCGTGGCAA TAGGGAGGGG GAAAGCGAAA
  781 GTCCCGGAAA GGAGCTGACA GGTGGTGGCA ATGCCCCAAC CAGTGGGGGT TGCGTCAGCA
  841 AACACAGTGC ACACCACGCC ACGTTGCCTG ACAACGGGGC CACAACTCCT CATAAAGAGA
  901 CAGCAACCAG GATTTATACA GAGGAGAAAT GAAAGCCATA CGGGAGCATA GCATGATACA
  961 AAGCATAAAG CAGCGTATCA CATAGCGTAA GAGCACATAG TAGATACAGT CATCTTCACA
 1021 ATTTGTATCA GAGTGATATC GATAGCTGAT TCTCGATGTA CTCTAGAGGA TCCTTACTTA
 1081 GGTAACCGGG AGCCATGTCA CGTCAATCGT CAGAAGCTCG CAGCAGCATT CATGTTCAAG
1141 TCGTTCAG
//
Primer: SV40pro_F
Sequence file: RDB12875_A5D2f.seq check
>12875_12875_A5D2_2_SV40pro_F_H07_22.ab1
    1 ACTTGGCTTG GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
  901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
  961 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
 1021 TAATGAATCG GGCCAACGCG CCGGGGAGAG GCGGTTTTGC GTATTGGGCG CTCTTTCCGC
 1081 TTTCTCCGCT CACTGACTCG CTGCCGCCTC GCTCGCTCGG CTTGCGCGAG CGGTATCAGG
1141 CCTCAACTTC AAAAAAGGGC GGTTAAATAT AACG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.01.22

GNP_filter3_RDBDEP_html_191130.pl