Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSIV-RfA-TRE-EF-KT

Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by EF-1 alpha promoter.

Sequence (full)	RDB12872zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 付加的提供条件： 利用者は、hKO1蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12872_A4Krp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-N_R Region: EF1 alpha pro 5', tight TRE pro, attR1, lac UV5 pro Sequence file: RDB12872_A4Kri.seq
>12872_12872_B1HjF_2_EF1a-N_R_A03_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNGG CGNNGTGCGC TCTGCCNNCT GNCGGGCACC GGNNCCTCAC    61 GAAGCTTGAA TTCGGCCCTT TCGTCTTCAA TCGAGTTTAC TCCCTATCAG TGATAGAGAA   121 CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGAT GTCGAGTTTA CTCCCTATCA   181 GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGTT   241 TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGTTTATC CCTATCAGTG ATAGAGAACG   301 TATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGGTAGG CGTGTACGGT   361 GGGAGGCCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCGCT AGCATCACAA   421 GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT   481 AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT   541 GGCGGCCGCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATAATGTGTG   601 GATTTTGAGT TAGGATCCGT CGAGATTTTC NGGAGCTAAG GAAGCTAAAA TGGAGAAAAA   661 AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC ATTTTGAGGC   721 ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA TTACGGCNNT   781 TTTTTAAAGA CCGNNAAAGA AAANTAANNA CAAGTTTTAT CNNGNCTTNN NNTCACNTTC   841 TTGCCCGCCT GATGNANNGC TCATCCNNGN ANTTCCGNNN NNNNNNGAAN NNANGGGTGA   901 ANCNGGNGNT NNNNGGNNNT NAGTGNTTCA NNNNNNTGNT TANNNNGNTT TTNCANGANC 961 NAANNNNNNN NNGNTTTTCN TCNCNNNTGN AANNNGAATN NNNNNNNNN //
Primer: EF1a-C_F Region: EF1 alpha pro, hKO, Sequence file: RDB12872_A4Krj.seq
>12872_12872_A4Kr_2_EF1a-C_F_B02_05_ABI24.ab1     1 NNNNNNNNNN NTTTTGNNNT NGGNNCTTGG TTNNTTCTCA AGCCTCAGAC AGTGGTTCAA    61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCCACCAT GGTGAGCGTG   121 ATCAAGCCCG AGATGAAGAT GAAGTACTTC ATGGACGGCA GCGTGAACGG CCACGAGTTC   181 ACCGTGGAGG GCGAGGGCAC CGGCAAGCCC TACGAGGGCC ACCAGGAGAT GACCCTGAGG   241 GTGACAATGG CCAAGGGCGG CCCCATGCCC TTCAGCTTCG ACCTGGTGAG CCACACCTTC   301 TGCTACGGCC ACAGGCCCTT CACCAAGTAC CCCGAGGAGA TCCCCGACTA CTTCAAGCAG   361 GCCTTCCCCG AGGGCCTGAG CTGGGAGAGG AGCCTCCAGT TCGAGGACGG CGGCTTCGCC   421 GCCGTGAGCG CCCACATCAG CCTGAGGGGC AACTGCTTCG AGCACAAGAG CAAGTTCGTG   481 GGCGTGAACT TCCCCGCCGA CGGCCCCGTG ATGCAGAACC AGAGCAGCGA CTGGGAGCCC   541 AGCACCGAGA AGATCACCAC CTGCGACGGC GTGCTGAAGG GCGACGTGAC CATGTACCTG   601 AAGCTGGCCG GCGGCGGCAA CCACAAGTGC CAGTTCAAGA NCACCTACAA GGCCGCCAAG   661 AAGATCCTGA AGANGCCCCA GANCCACTTC ATCGGCCACA GGCTGGTGAG GAAGACCNAN   721 GGCNACATCA CCGANCTGGT GGANGANGCC NNGGCCCACN GCGANGGCAN ANGAAGTCTT   781 CTAACATGCN GTGACGTGGN NNANANTCCC GGCCCNATGT CTAGACNGNN NNAGANNNAA   841 GTCNTNAANN GCGCTCNNGN NNNTNNNTCN NNNGNNNNCN GNATNNNANN NNCNGANNAN 901 NNNNNAACNN NNNNNAANNN NNNGNNNNTN NNNNNNNNN //
Primer: tTA-Ad R Region: rtTA-Advanced 3',T2A,hKO Sequence file: RDB12872_A4Krk.seq
> //
Primer: CMV_Forward Region: CMV pro, 3', 5' LTR, HIV-1 psi Sequence file: RDB12872_A4Krl.seq
>12872_12872_B1HjF_2_CMV_Forward_E03_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNTG NNGCNANNGG GCGGTANGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG   121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT   181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT   241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA   301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG   361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG   421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC   481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG   541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA   601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCANA NNAACTTAGA TCNNTATATA   661 ATACAGTANC NACCCTCTAT TGTGTGCATC NAAAGGATAG AGATAAAAGA CACCAAGGAA   721 NGCTTTANAC AANNNNNNNN GAAGAGCAAA ACNNNGTAAG ACCNCCGCAC AGCANNCGGN   781 CNGCTGATCN TNNANANNTG GANNGNNNNN NTATGANGGN ACAATTGGNA GAANNNGNNN   841 TTANNTNNAN NNNANANNNN NTANANNNNT NGAANCNANN TNNNNNNNNA NNNNNNNNNN //
Primer: PRE_rev Region: PRE,rtTA-Advanced 3' Sequence file: RDB12872_A4Krm.seq
>12872_12872_B1HjF_PRE_rev_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTA GTTAAGAATA CCAGTCAATC TTTCACAAAT    61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGAT   121 CCTTACTTAG TTACCCGGGG AGCATGTCAA GGTCAAAATC GTCAAGAGCG TCAGCAGGCA   181 GCATATCAAG GTCAAAGTCG TCAAGGGCAT CGGCTGGGAG CATGTCTAAG TCAAAATCGT   241 CAAGGGCGTC GGCCGGCCCG CCGCTTTCGC ACTTTAGCTG TTTCTCCAGG CCACATATGA   301 TTAGTTCCAG GCCGAAAAGG AAGGCAGGTT CGGCTCCCTG CCGGTCGAAC AGCTCAATTG   361 CTTGTCTCAG AAGTGGGGGC ATAGAATCGG TGGTAGGTGT CTCTCTTTCC TCTTTTGCTA   421 CTTGATGCTC CTGTTCCTCC AATACGCAGC CCAGTGTAAA GTGGCCCACG GCGGACAGAG   481 CGTACAGTGC GTTCTCCAGG GAGAAGCCTT GCTGACACAG GAACGCGAGC TGATTTTCCA   541 GGGTTTCGTA CTGTTTCTCT GTTGGGCGGG TGCCGAGATG CACTTTAGCC CCGTCGCGAT   601 GTGAGAGGAG AGCACAGCGG ANNGACTTGN CGTTGTTCCG CAGAAAGTCT TGCCNTGACT   661 CGCCTTCCAG GGGGCAGAAN TGGGTATGAT GCCNGTCCAG CATCTCGNNN NNNNNGGNNA 721 TCNNNNNNGN NCNNNNTNNN NNNNNNN //
Primer: bGH_rev3 Region: bGH pA 5', 3' LTR, loxP Sequence file: RDB12872_A4Krn.seq
>12872_12872_B1HjF_2_bGH_rev3_F03_06_ABI08.ab1     1 NNNNNNNNNN NNNNGNNNNN NNGGNNGGGC ANNNNNNGNN GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA   121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA   181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT   241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGAATA ACTTCGTATA   301 ATGTATGCTA TACGAAGTTA TGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG   361 TCCNNNNNNT TCTTNNNNNN AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT   421 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA   481 NGCNCAANCN NNNTTGGTAG CNNNNNTATT GNNNNNTGNG NNTGCTCCAT GTTTTTCTAG   541 GTNNNNNNGG TATCNATGCN GGGAGGCGGN NNNNANGGNN NNNNNANTCN NCTGAGGGCN 601 NNNGNNNNNN CNCNNANNAN GCNNNNNANN NNNNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, Zeo Sequence file: RDB12872_A4Kro.seq
>12872_12872_B1HjF_2_SV40pro_ori_F_G03_07_ABI08.ab1     1 NNNNNNNNNN NNNNGNNGCC NCNGCCTCTG NNCTANTCNN GAAGTAGTGA GGAGGCTTTT    61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG   121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA   181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA   241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA   301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA   361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA   421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC   481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG   541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC   601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT   661 CCAGCGCGGG GATCTCATGC TGGANTTCTT CGCCCACCNN NNNTTGTTTA NTGCANCNTT   721 ATNANTGGTT ACNAATAAAA GCAANNAGCA TCANNAATTT TCACAAAATA AAGCATTTTT   781 TTCACTGCAT NCNANNTTNG NGGNTTGTCC AAACTCATCA ANGNNNNNNN NNCANGTNNG 841 NNNNACNNNN NNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, Zeo Sequence file: RDB12872_A4Krp.seq
>12872_12872_B1HjF_2_SV40pro_ori_F_G03_07_ABI08.ab1     1 NNNNNNNNNN NNNNGNNGCC NCNGCCTCTG NNCTANTCNN GAAGTAGTGA GGAGGCTTTT    61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG   121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA   181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA   241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA   301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA   361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA   421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC   481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG   541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC   601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT   661 CCAGCGCGGG GATCTCATGC TGGANTTCTT CGCCCACCNN NNNTTGTTTA NTGCANCNTT   721 ATNANTGGTT ACNAATAAAA GCAANNAGCA TCANNAATTT TCACAAAATA AAGCATTTTT   781 TTCACTGCAT NCNANNTTNG NGGNTTGTCC AAACTCATCA ANGNNNNNNN NNCANGTNNG 841 NNNNACNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.10.11

GNP_filter3_RDBDEP_html_221010.pl