RDB12872 - CSIV-RfA-TRE-EF-KT

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CSIV-RfA-TRE-EF-KT (#RDB12872)

Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by EF-1 alpha promoter.

Clone info.	Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by EF-1 alpha promoter. Please note that the TRE and EF promoters are placed in back-to-back orientation and this construct contains loxP.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CSIV-RfA-TRE-EF-KT (Plasmid)
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence (full)	RDB12872zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12872	CSIV-RfA-TRE-EF-KT	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-RfA-TRE-EF-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12872).

Reference section:

Iida, T., Iwanami, A., Sanosaka, T., Kohyama, J., Miyoshi, H., Nagoshi, N., Kashiwagi, R., Toyama, Y., Matsumoto, M., Nakamura, M., Okano, H., Whole-Genome DNA Methylation Analyses Revealed Epigenetic Instability in Tumorigenic Human iPS Cell-Derived Neural Stem/Progenitor Cells. Stem Cells 35 (5): 1316-1327 (2017). PMID 28142229. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12872_A4Krp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-N_R (Pr0024) Region: EF1 alpha pro 5', tight TRE pro, attR1, lac UV5 pro Sequence file: RDB12872_A4Kri.seq
>12872_12872_B1HjF_2_EF1a-N_R_A03_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNGG CGNNGTGCGC TCTGCCNNCT GNCGGGCACC GGNNCCTCAC 61 GAAGCTTGAA TTCGGCCCTT TCGTCTTCAA TCGAGTTTAC TCCCTATCAG TGATAGAGAA 121 CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGAT GTCGAGTTTA CTCCCTATCA 181 GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGTT 241 TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGTTTATC CCTATCAGTG ATAGAGAACG 301 TATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGGTAGG CGTGTACGGT 361 GGGAGGCCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCGCT AGCATCACAA 421 GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT 481 AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT 541 GGCGGCCGCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATAATGTGTG 601 GATTTTGAGT TAGGATCCGT CGAGATTTTC NGGAGCTAAG GAAGCTAAAA TGGAGAAAAA 661 AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC ATTTTGAGGC 721 ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA TTACGGCNNT 781 TTTTTAAAGA CCGNNAAAGA AAANTAANNA CAAGTTTTAT CNNGNCTTNN NNTCACNTTC 841 TTGCCCGCCT GATGNANNGC TCATCCNNGN ANTTCCGNNN NNNNNNGAAN NNANGGGTGA 901 ANCNGGNGNT NNNNGGNNNT NAGTGNTTCA NNNNNNTGNT TANNNNGNTT TTNCANGANC 961 NAANNNNNNN NNGNTTTTCN TCNCNNNTGN AANNNGAATN NNNNNNNNN //
Primer: EF1a-C_F (Pr0168) Region: EF1 alpha pro, hKO, Sequence file: RDB12872_A4Krj.seq
>12872_12872_A4Kr_2_EF1a-C_F_B02_05_ABI24.ab1 1 NNNNNNNNNN NTTTTGNNNT NGGNNCTTGG TTNNTTCTCA AGCCTCAGAC AGTGGTTCAA 61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCCACCAT GGTGAGCGTG 121 ATCAAGCCCG AGATGAAGAT GAAGTACTTC ATGGACGGCA GCGTGAACGG CCACGAGTTC 181 ACCGTGGAGG GCGAGGGCAC CGGCAAGCCC TACGAGGGCC ACCAGGAGAT GACCCTGAGG 241 GTGACAATGG CCAAGGGCGG CCCCATGCCC TTCAGCTTCG ACCTGGTGAG CCACACCTTC 301 TGCTACGGCC ACAGGCCCTT CACCAAGTAC CCCGAGGAGA TCCCCGACTA CTTCAAGCAG 361 GCCTTCCCCG AGGGCCTGAG CTGGGAGAGG AGCCTCCAGT TCGAGGACGG CGGCTTCGCC 421 GCCGTGAGCG CCCACATCAG CCTGAGGGGC AACTGCTTCG AGCACAAGAG CAAGTTCGTG 481 GGCGTGAACT TCCCCGCCGA CGGCCCCGTG ATGCAGAACC AGAGCAGCGA CTGGGAGCCC 541 AGCACCGAGA AGATCACCAC CTGCGACGGC GTGCTGAAGG GCGACGTGAC CATGTACCTG 601 AAGCTGGCCG GCGGCGGCAA CCACAAGTGC CAGTTCAAGA NCACCTACAA GGCCGCCAAG 661 AAGATCCTGA AGANGCCCCA GANCCACTTC ATCGGCCACA GGCTGGTGAG GAAGACCNAN 721 GGCNACATCA CCGANCTGGT GGANGANGCC NNGGCCCACN GCGANGGCAN ANGAAGTCTT 781 CTAACATGCN GTGACGTGGN NNANANTCCC GGCCCNATGT CTAGACNGNN NNAGANNNAA 841 GTCNTNAANN GCGCTCNNGN NNNTNNNTCN NNNGNNNNCN GNATNNNANN NNCNGANNAN 901 NNNNNAACNN NNNNNAANNN NNNGNNNNTN NNNNNNNNN //
Primer: tTA-Ad R (Pr0542) Region: rtTA-Advanced 3',T2A,hKO Sequence file: RDB12872_A4Krk.seq
>12872_12872_B1HjF_tTA-AdR_E09_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGANTTTGCT CTTGTCNNGT CTAGACATAG GGCCGGGATT 61 CTCCTCCACG TCACCGCATG TTAGAAGACT TCCTCTGCCC TCGCAGTGGG CCACGGCGTC 121 CTCCACCAGC TCGGTGATGT TGCCCTCGGT CTTCCTCACC AGCCTGTGGC CGATGAAGTG 181 GCTCTGGGGC ATCTTCAGGA TCTTCTTGGC GGCCTTGTAG GTGGTCTTGA ACTGGCACTT 241 GTGGTTGCCG CCGCCGGCCA GCTTCAGGTA CATGGTCACG TCGCCCTTCA GCACGCCGTC 301 GCAGGTGGTG ATCTTCTCGG TGCTGGGCTC CCAGTCGCTG CTCTGGTTCT GCATCACGGG 361 GCCGTCGGCG GGGAAGTTCA CGCCCACGAA CTTGCTCTTG TGCTCGAAGC AGTTGCCCCT 421 CAGGCTGATG TGGGCGCTCA CGGCGGCGAA GCCGCCGTCC TCGAACTGGA GGCTCCTCTC 481 CCAGCTCAGG CCCTCGGGGA AGGCCTGCTT GAAGTAGTCG GGGATCTCCT CGGGGTACTT 541 GGTGAANGGC CTGTGGCCGT ANCANAANGT GTGNNNNACC ANGTCGAANC TGANNGGCNT 601 GGGGCCGCNC TTGGNCNNNG TCACCCTNNN NGTCATCTCC NGNNGNCCCT CGTANGNNNN 661 GNNNGTGCCC NNNNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 3', 5' LTR, HIV-1 psi Sequence file: RDB12872_A4Krl.seq
>12872_12872_B1HjF_2_CMV_Forward_E03_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNTG NNGCNANNGG GCGGTANGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG 121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT 181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT 241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA 301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG 361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG 421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC 481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG 541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA 601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCANA NNAACTTAGA TCNNTATATA 661 ATACAGTANC NACCCTCTAT TGTGTGCATC NAAAGGATAG AGATAAAAGA CACCAAGGAA 721 NGCTTTANAC AANNNNNNNN GAAGAGCAAA ACNNNGTAAG ACCNCCGCAC AGCANNCGGN 781 CNGCTGATCN TNNANANNTG GANNGNNNNN NTATGANGGN ACAATTGGNA GAANNNGNNN 841 TTANNTNNAN NNNANANNNN NTANANNNNT NGAANCNANN TNNNNNNNNA NNNNNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,rtTA-Advanced 3' Sequence file: RDB12872_A4Krm.seq
>12872_12872_B1HjF_PRE_rev_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTA GTTAAGAATA CCAGTCAATC TTTCACAAAT 61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGAT 121 CCTTACTTAG TTACCCGGGG AGCATGTCAA GGTCAAAATC GTCAAGAGCG TCAGCAGGCA 181 GCATATCAAG GTCAAAGTCG TCAAGGGCAT CGGCTGGGAG CATGTCTAAG TCAAAATCGT 241 CAAGGGCGTC GGCCGGCCCG CCGCTTTCGC ACTTTAGCTG TTTCTCCAGG CCACATATGA 301 TTAGTTCCAG GCCGAAAAGG AAGGCAGGTT CGGCTCCCTG CCGGTCGAAC AGCTCAATTG 361 CTTGTCTCAG AAGTGGGGGC ATAGAATCGG TGGTAGGTGT CTCTCTTTCC TCTTTTGCTA 421 CTTGATGCTC CTGTTCCTCC AATACGCAGC CCAGTGTAAA GTGGCCCACG GCGGACAGAG 481 CGTACAGTGC GTTCTCCAGG GAGAAGCCTT GCTGACACAG GAACGCGAGC TGATTTTCCA 541 GGGTTTCGTA CTGTTTCTCT GTTGGGCGGG TGCCGAGATG CACTTTAGCC CCGTCGCGAT 601 GTGAGAGGAG AGCACAGCGG ANNGACTTGN CGTTGTTCCG CAGAAAGTCT TGCCNTGACT 661 CGCCTTCCAG GGGGCAGAAN TGGGTATGAT GCCNGTCCAG CATCTCGNNN NNNNNGGNNA 721 TCNNNNNNGN NCNNNNTNNN NNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA 5', 3' LTR, loxP Sequence file: RDB12872_A4Krn.seq
>12872_12872_B1HjF_2_bGH_rev3_F03_06_ABI08.ab1 1 NNNNNNNNNN NNNNGNNNNN NNGGNNGGGC ANNNNNNGNN GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA 121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA 181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT 241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGAATA ACTTCGTATA 301 ATGTATGCTA TACGAAGTTA TGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG 361 TCCNNNNNNT TCTTNNNNNN AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT 421 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA 481 NGCNCAANCN NNNTTGGTAG CNNNNNTATT GNNNNNTGNG NNTGCTCCAT GTTTTTCTAG 541 GTNNNNNNGG TATCNATGCN GGGAGGCGGN NNNNANGGNN NNNNNANTCN NCTGAGGGCN 601 NNNGNNNNNN CNCNNANNAN GCNNNNNANN NNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, Zeo Sequence file: RDB12872_A4Kro.seq
>12872_12872_B1HjF_2_SV40pro_ori_F_G03_07_ABI08.ab1 1 NNNNNNNNNN NNNNGNNGCC NCNGCCTCTG NNCTANTCNN GAAGTAGTGA GGAGGCTTTT 61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG 121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA 181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA 241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA 301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA 361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA 421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC 481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG 541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC 601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 661 CCAGCGCGGG GATCTCATGC TGGANTTCTT CGCCCACCNN NNNTTGTTTA NTGCANCNTT 721 ATNANTGGTT ACNAATAAAA GCAANNAGCA TCANNAATTT TCACAAAATA AAGCATTTTT 781 TTCACTGCAT NCNANNTTNG NGGNTTGTCC AAACTCATCA ANGNNNNNNN NNCANGTNNG 841 NNNNACNNNN NNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, Zeo Sequence file: RDB12872_A4Krp.seq
>12872_12872_B1HjF_2_SV40pro_ori_F_G03_07_ABI08.ab1 1 NNNNNNNNNN NNNNGNNGCC NCNGCCTCTG NNCTANTCNN GAAGTAGTGA GGAGGCTTTT 61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG 121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA 181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA 241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA 301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA 361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA 421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC 481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG 541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC 601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 661 CCAGCGCGGG GATCTCATGC TGGANTTCTT CGCCCACCNN NNNTTGTTTA NTGCANCNTT 721 ATNANTGGTT ACNAATAAAA GCAANNAGCA TCANNAATTT TCACAAAATA AAGCATTTTT 781 TTCACTGCAT NCNANNTTNG NGGNTTGTCC AAACTCATCA ANGNNNNNNN NNCANGTNNG 841 NNNNACNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Iida, T., Whole-Genome DNA Methylation Analyses Revealed Epigenetic Instability in Tumorigenic Human iPS Cell-Derived Neural Stem/Progenitor Cells. Stem Cells 35 (5): 1316-1327 (2017). PMID 28142229. [link to RRC of NBRP]
reference	Kojima, K., Selective Ablation of Tumorigenic Cells Following Human Induced Pluripotent Stem Cell-Derived Neural Stem/Progenitor Cell Transplantation in Spinal Cord Injury. Stem Cells Transl. Med. 8 (3): 260-270 (2019). PMID 30485733.
reference	Iwasawa, C., Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells. Int. J. Mol. Sci. 20 (4). pii: E810 (2019). PMID 30769780.
reference	Yoshimatsu, S., Evaluating the efficacy of small molecules for neural differentiation of common marmoset ESCs and iPSCs. Neurosci. Res. pii: S0168-0102 (19) 30262-7 (2019). PMID 31586586.
reference	Kwon, S.J., Role of MEK partner-1 in cancer stemness through MEK/ERK pathway in cancerous neural stem cells, expressing EGFRviii. Mol. Cancer 16 (1): 140 (2017). PMID 28830458.

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