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CSIV-RfA-TRE-EF-KT

Backbone vector of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by EF-1 alpha promoter.

Catalog number RDB12872
Resource name CSIV-RfA-TRE-EF-KT
Clone info. Backbone vector of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by EF-1 alpha promoter. Please note that the TRE and EF promoters are placed in back-to-back orientation and this construct contains loxP.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-RfA-TRE-EF-KT (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering Please visit Ordering instruction. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12872 CSIV-RfA-TRE-EF-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Iida, T., Whole-Genome DNA Methylation Analyses Revealed Epigenetic Instability in Tumorigenic Human iPS Cell-Derived Neural Stem/Progenitor Cells. Stem Cells 35 (5): 1316-1327 (2017). PMID 28142229.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12872_A4Krp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-N_R
Sequence file: RDB12872_A4Kra.seq check
>12872_12872_A4Kr_2_EF1a-N_R_H01_22_ABI24.ab1
    1 ATTCTGGGGG GGGCTGTGGG GCGATGTGCG CTCTGCCCAC TGACGGGCAC CGGAGCCTCA
   61 CGAAGCTTGA ATTCGGCCCT TTCGTCTTCA ATCGAGTTTA CTCCCTATCA GTGATAGAGA
  121 ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGA TGTCGAGTTT ACTCCCTATC
  181 AGTGATAGAG AACGTATGTC GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGTCGAGT
  241 TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAT CCCTATCAGT GATAGAGAAC
  301 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GTCGAGGTAG GCGTGTACGG
  361 TGGGAGGCCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCGC TAGCATCACA
  421 AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT
  481 TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA
  541 TGGCGGCCGC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT
  601 GGATTTTGAG TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA
  661 AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG
  721 CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT
  781 TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT
  841 GCCCGCCTGA TGAATGCTCA TCCCGGAAAT TCCGTATGGC AATGAAAGAC GGGTGAAGCT
  901 GGTGATATGG GGATAGTGTT TCACCTTGTT AACACGTTTT CCAATGAGCA ACCTGAAACG
  961 GTTTTCATCG CTCTTGGAGT GATAACCACG ACGGAATTCC GCAGGTTTCT ACACATAATT
1021 ATTCGCAGGA ATGTTGGCGG TGTTACCGTG AAACATGGCA TATCCATAAG GTTATTGAG
//
Primer: cPPT+CTS_V2
Sequence file: RDB12872_A4Krb.seq check
>12872_12872_A8Ff_cPPT+CTS_V2_F_D01_04_ABI08.ab1
    1 GGAAGAAATT CCAAAACTTA AAGAAATTAC AAAAACAAAT TACAAAAATT CAAGAATTTT
   61 CGGGTTTATT ACAGGGACAG CAGAAATTCA CTTTGAATTC TAGCTCGAGA CCGGTAGCAT
  121 CACCACTTTG TACAAGAAAG CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT
  181 AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC
  241 ACTATGGTCG ACCTGCAGAC TGGCTGTGTA TAAGGGAGCC TGACATTTAT ATTCCCCAGA
  301 ACATCAGGTT AATGGCGTTT TTGATGTCAT TTTCGCGGTG GCTGAGATCA GCCACTTCTT
  361 CCCCGATAAC GGAGACCGGC ACACTGGCCA TATCGGTGGT CATCATGCGC CAGCTTTCAT
  421 CCCCGATATG CACCACCGGG TAAAGTTCAC GGGAGACTTT ATCTGACAGC AGACGTGCAC
  481 TGGCCAGGGG GATCACCATC CGTCGCCCGG GCGTGTCAAT AATATCACTC TGTACATCCA
  541 CAAACAGACG ATAACGGCTC TCTCTTTTAT AGGTGTAAAC CTTAAACTGC ATTTCACCAG
  601 CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAATAA ACCGGGCGAC CTCAGCCATC
  661 CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG GCACGCAGAC GACGGGCTTC ATTCTGCATG
  721 GTTGTGCTTA CCAGACCGGA GATATTGACA TCATATATGC CTTGAGCAAC TGATAGCTGT
  781 CGCTGTCAAC TGTCACTGTA AATACGCTGC TTCATAGCAT ACCTCTTTTT GACATACTTC
  841 GGGGTATACA TATCAGTATA TATTTCTTAT ACCGCAAAAA TCAGCGCGCA AAATACGCAT
  901 ACTGTTATCT GGGCTTTTAG TAAGCCGGAT CCACGCGTTT ACGCCCCGCC CCTGCCCACT
  961 CAATCGCAGT ACTGGTTGTA ATTCAATTAA AGCATTCTGC CGGACCATGG GAAGCCCATC
 1021 ACAACGGGCA TGGATGAAAC CCTGATCGGC CAGCGGCATC AGCAACCGTT GTTCGGCCTT
 1081 TGCCGTAATA TATTTGCCCA ATGGGCTGAA AACTGGGTCG AGCAGCTTGG TCCAATAATT
1141 GGGCCCACCG TTTTTAAAAT ATCA
//
Primer: EF1a-C_F
Sequence file: RDB12872_A4Krc.seq check
>12872_12872_A8Ff_EF1a-C_F_F01_06_ABI08.ab1
    1 CCGTGGGGAA ATTGCCCCCT TTTTTTGAGT TTTGGGATCT TGGTTCATTC TCAAGGCCTC
   61 AGAACAGGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAACAC GCTACCGGTC
  121 CACCATGGTG AGCGTGATCA AGCCCGAGAT GAAGATGAAG TACTTCATGG ACGGCAGCGT
  181 GAACGGCCAC GAGTTCACCG TGGAGGGCGA GGGCACCGGC AAGCCCTACG AGGGCCACCA
  241 GGAGATGACC CTGAGGGTGA CAATGGCCAA GGGCGGCCCC ATGCCCTTCA GCTTCGACCT
  301 GGTGAGCCAC ACCTTCTGCT ACGGCCACAG GCCCTTCACC AAGTACCCCG AGGAGATCCC
  361 CGACTACTTC AAGCAGGCCT TCCCCGAGGG CCTGAGCTGG GAGAGGAGCC TCCAGTTCGA
  421 GGACGGCGGC TTCGCCGCCG TGAGCGCCCA CATCAGCCTG AGGGGCAACT GCTTCGAGCA
  481 CAAGAGCAAG TTCGTGGGCG TGAACTTCCC CGCCGACGGC CCCGTGATGC AGAACCAGAG
  541 CAGCGACTGG GAGCCCAGCA CCGAGAAGAT CACCACCTGC GACGGCGTGC TGAAGGGCGA
  601 CGTGACCATG TACCTGAAGC TGGCCGGCGG CGGCAACCAC AAGTGCCAGT TCAAGACCAC
  661 CTACAAGGCC GCCAAGAAGA TCCTGAAGAT GCCCCAGAGC CACTTCATCG GCCACAGGCT
  721 GGTGAGGGAA GACCGAGGGC AACATCACCG AGCTGGTGGA GGACGCCGTG GCCCACTGCG
  781 GAGGGCAGAG GAAGTCTTCT AAACATGCGG GTGACGTGGA GGAGAATCCC GGCCCTAATG
  841 TCTAGGACTG GACAAGAGCA AAAGTCATAA AACGGCGCTC TGGAATTACT CAAATGGGAG
  901 ATCGGTATCG AAGGCCTGAC GACAAAGGAA ACCTCGCTCA AAAAGCTGGG AGTTGAAGCA
  961 GCCTACCCTG TACTGGCCAC GTTGAAGAAA CAAAGCGGGG CTCCTGCCTC GAATGCCCTG
 1021 GCCCAATTCG AAGAATGCCT GGGAACAAGG CCATCATACC CCAACTTTCT TGGCCTCCTG
 1081 GAGGGCGAGG TCATTGGCAA GAACTTTTCT TGCGGAAACA ACGGCCAAGT TCAATTCCGC
 1141 TGGTTGCCTC CTCCATCTCC AATCGCGACA CGGGCTTAAG ATGCCGATTC TATCCGAGAC
1201 TC
//
Primer: hKO_F
Sequence file: RDB12872_A4Krd.seq check
>12872_12872_A8Ff_hKO_F_F01_06_ABI08.ab1
    1 CTTTATCGGG GCCAAGGGCT GGTGAGGAAG ACCGAGGGCA ACATCACCGA GCTGGTGGAG
   61 GACGCCGTGG CCCACTGCGA GGGCAGAGGA AGTCTTCTAA CATGCGGTGA CGTGGAGGAG
  121 AATCCCGGCC CTATGTCTAG ACTGGACAAG AGCAAAGTCA TAAACGGCGC TCTGGAATTA
  181 CTCAATGGAG TCGGTATCGA AGGCCTGACG ACAAGGAAAC TCGCTCAAAA GCTGGGAGTT
  241 GAGCAGCCTA CCCTGTACTG GCACGTGAAG AACAAGCGGG CCCTGCTCGA TGCCCTGCCA
  301 ATCGAGATGC TGGACAGGCA TCATACCCAC TTCTGCCCCC TGGAAGGCGA GTCATGGCAA
  361 GACTTTCTGC GGAACAACGC CAAGTCATTC CGCTGTGCTC TCCTCTCACA TCGCGACGGG
  421 GCTAAAGTGC ATCTCGGCAC CCGCCCAACA GAGAAACAGT ACGAAACCCT GGAAAATCAG
  481 CTCGCGTTCC TGTGTCAGCA AGGCTTCTCC CTGGAGAACG CACTGTACGC TCTGTCCGCC
  541 GTGGGCCACT TTACACTGGG CTGCGTATTG GAGGAACAGG AGCATCAAGT AGCAAAAGAG
  601 GAAAGAGAGA CACCTACCAC CGATTCTATG CCCCCACTTC TGAGACAAGC AATTGAGCTG
  661 TTCGACCGGC AGGGAGCCGA ACCTGCCTTC CTTTTCGGCC TGGAACTAAT CATATGTGGC
  721 CTGGAGAAAC AGCTAAAGTG CGAAAGCGGC GGGCCGGCCG ACGCCCTTGA CGATTTTGAC
  781 TTAGACATGC TCCCAGCCGA TGCCCTTGAC GACTTTGACC TTGATATGCT GCCTGCTGAC
  841 GCTCTTGACG ATTTTGACCT TGACATGCTC CCCGGGTAAC TAAGTAAGGA TCCTCTAGAG
  901 TTAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG
  961 ATTGACTGGT ATTCTTAACT ATGTTGCTCC CTTTTACGCT ATGTGGATAC GCTGCTTTAA
 1021 TGCCCTTGTA TCATGCTATT GCTTTCCCGT ATGGCTTTCA TTTTCTCTCC ATGTATAAAT
 1081 CCCTGGTTGC TGTCTCTTTA TGGAGAGTTG TGGCCCGTGT CAGCAACGTT GCCGTGATGT
1141 GCACCTGGTG ATTTGGCTGA ACGCCAACCC CCCCCCAC
//
Primer: CMV-Forward
Sequence file: RDB12872_A4Kre.seq check
>12872_12872_A4Kr_2_CMV-Forward_B03_06_ABI24.ab1
    1 AACAACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GCGGCCGCTG
  781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAAATTGAAC CATTAGGAGT AGCACCCACC AAAGGCAAAG AGAAGAGTGG
  901 GTGCAGAGAG AAAAAAAGAG CAGTGGGAAT AGGAGCTTTT GTTCCTTGGG TTTCTTTGGG
  961 GAGCAGCAGG GAAAGCACTA TGGGCGCAGC GGTCAAATGG ACGGCTGACG GGTTACAGCC
1021 AAGACCATAA TGTCTGTATA GTTGCAGCCA GGCAGAACCA AATTTGGCCT GTGGAG
//
Primer: BGH_rev2
Sequence file: RDB12872_A4Krf.seq check
>12872_12872_A4Kr_2_BGH_rev2_C03_09_ABI24.ab1
    1 CTATCCAGGA TAGTAGGCAC GGGGGAGGGG CAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGAAT AACTTCGTAT
  301 AATGTATGCT ATACGAAGTT ATGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA
  361 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC
  421 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC
  481 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA
  541 GGTCTCGACG GTATCGATGC GGGGAGGCGG CCCAAAGGGA GATCCGACTC GTCTGAGGGC
  601 GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG
  661 CTGGATTGAG GGCCGAAGGG ACGTAGCAGA AGGACGTCCC GCGCAGAATC CAGGTGGCAA
  721 CACAGGCGAG CAGCCATGGA AAGGACGTCA GCTTCCCCGA CAACACCACG GAATTGTCAG
  781 TGCCCAACAG CCGAGCCCCT GTCCAGCAGC GGGCAAAGGC AGGCGGCGAT GAGTTCCGCC
  841 GTGGCAATAG GGAGGGGGAA AGCGAAAGTC CCGGAAAGGA GCTGACAGGT GGTGGCAATG
  901 CCCCAACCAG TGGGGGGTTG CGTCAGCAAA CACAGTGCAC ACCACGCCAC GTTGCCTGAC
  961 AACGGCCACA ACTCCTCATA AAGAGACAGC AACCAGGATT TATACAGGAG GAGAAAATGA
 1021 AAGCCATACG GAAGCAATAG CATGAATACC AAAGGGCATA AGCAGCGTAT CCACTTAGCC
1081 GTTAAAGGAG CCAACAATAG TTCAGAATAA CCCAAGGTCA CAGATTCGCT
//
Primer: SV40pro_F
Sequence file: RDB12872_A4Krg.seq check
>12872_12872_A4Kr_2_SV40pro_F_D03_12_ABI24.ab1
    1 ACGGGCCCGT ACTTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA
  121 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG
  181 TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG
  241 ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG
  301 GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG
  361 GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC
  421 ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG
  481 CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG
  541 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
  601 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
  661 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
  721 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
  781 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
  841 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACAACAACAT
  901 ACGAGCCGGA AGCATAAAAG TGTAAAGCCC TGGGGGTGCC CTAATGAGTT GAGCTAAACT
  961 CACATTTAAA TTGCGTTTGC CGCTCACTGC CCGCTTTCCA GTTCGGGGAA AACCCTTGTT
 1021 CGTGCCAGCT TGCCATTAAT GAAATTCGGC TAACGCGTCG GTGCAGAAGC CGGTTGCCGT
1081 ATTTGGCGCT CTTCCGGCAT TCCTTCGACT TCACATGGAC AC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Iida, T., Whole-Genome DNA Methylation Analyses Revealed Epigenetic Instability in Tumorigenic Human iPS Cell-Derived Neural Stem/Progenitor Cells. Stem Cells 35 (5): 1316-1327 (2017). PMID 28142229.
reference Kojima, K., Selective Ablation of Tumorigenic Cells Following Human Induced Pluripotent Stem Cell-Derived Neural Stem/Progenitor Cell Transplantation in Spinal Cord Injury. Stem Cells Transl. Med. 8 (3): 260-270 (2019). PMID 30485733.
reference Iwasawa, C., Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells. Int. J. Mol. Sci. 20 (4). pii: E810 (2019). PMID 30769780.
reference Yoshimatsu, S., Evaluating the efficacy of small molecules for neural differentiation of common marmoset ESCs and iPSCs. Neurosci. Res. pii: S0168-0102 (19) 30262-7 (2019). PMID 31586586.
reference Kwon, S.J., Role of MEK partner-1 in cancer stemness through MEK/ERK pathway in cancerous neural stem cells, expressing EGFRviii. Mol. Cancer 16 (1): 140 (2017). PMID 28830458.

2019.12.20

GNP_filter3_RDBDEP_html_191130.pl