Resource data sheet
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CSIV-RfA-TRE-EF-KT

Backbone vector of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by EF-1 alpha promoter.

Catalog number RDB12872
Resource name CSIV-RfA-TRE-EF-KT
Clone info. Backbone vector of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by EF-1 alpha promoter. Please note that the TRE and EF promoters are placed in back-to-back orientation and this construct contains loxP.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-RfA-TRE-EF-KT (Plasmid)
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12872 CSIV-RfA-TRE-EF-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Iida, T., Whole-Genome DNA Methylation Analyses Revealed Epigenetic Instability in Tumorigenic Human iPS Cell-Derived Neural Stem/Progenitor Cells. Stem Cells 35 (5): 1316-1327 (2017). PMID 28142229.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12872_A4Krp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-N_R RDB12872_A4Kra.seq
>12872_12872_A4Kr_2_EF1a-N_R_H01_22_ABI24.ab1
    1 ATTCTGGGGG GGGCTGTGGG GCGATGTGCG CTCTGCCCAC TGACGGGCAC CGGAGCCTCA
   61 CGAAGCTTGA ATTCGGCCCT TTCGTCTTCA ATCGAGTTTA CTCCCTATCA GTGATAGAGA
  121 ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGA TGTCGAGTTT ACTCCCTATC
  181 AGTGATAGAG AACGTATGTC GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGTCGAGT
  241 TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAT CCCTATCAGT GATAGAGAAC
  301 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GTCGAGGTAG GCGTGTACGG
  361 TGGGAGGCCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCGC TAGCATCACA
  421 AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT
  481 TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA
  541 TGGCGGCCGC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT
  601 GGATTTTGAG TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA
  661 AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG
  721 CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT
  781 TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT
  841 GCCCGCCTGA TGAATGCTCA TCCCGGAAAT TCCGTATGGC AATGAAAGAC GGGTGAAGCT
  901 GGTGATATGG GGATAGTGTT TCACCTTGTT AACACGTTTT CCAATGAGCA ACCTGAAACG
  961 GTTTTCATCG CTCTTGGAGT GATAACCACG ACGGAATTCC GCAGGTTTCT ACACATAATT
1021 ATTCGCAGGA ATGTTGGCGG TGTTACCGTG AAACATGGCA TATCCATAAG GTTATTGAG
//
Primer: cPPT+CTS_v2_F RDB12872_A4Krb.seq
>12872_12872_A4Kr_2_cPPT+CTS_v2_F_A02_02_ABI24.ab1
    1 TAGTGATACA ACTAAAGATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TCGGGTTTAT
   61 TACAGGGACA GCAGAAATTC ACTTTGAATT CTAGCTCGAG ACCGGTAGCA TCACCACTTT
  121 GTACAAGAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT
  181 TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGGTC
  241 GACCTGCAGA CTGGCTGTGT ATAAGGGAGC CTGACATTTA TATTCCCCAG AACATCAGGT
  301 TAATGGCGTT TTTGATGTCA TTTTCGCGGT GGCTGAGATC AGCCACTTCT TCCCCGATAA
  361 CGGAGACCGG CACACTGGCC ATATCGGTGG TCATCATGCG CCAGCTTTCA TCCCCGATAT
  421 GCACCACCGG GTAAAGTTCA CGGGAGACTT TATCTGACAG CAGACGTGCA CTGGCCAGGG
  481 GGATCACCAT CCGTCGCCCG GGCGTGTCAA TAATATCACT CTGTACATCC ACAAACAGAC
  541 GATAACGGCT CTCTCTTTTA TAGGTGTAAA CCTTAAACTG CATTTCACCA GCCCCTGTTC
  601 TCGTCAGCAA AAGAGCCGTT CATTTCAATA AACCGGGCGA CCTCAGCCAT CCCTTCCTGA
  661 TTTTCCGCTT TCCAGCGTTC GGCACGCAGA CGACGGGCTT CATTCTGCAT GGTTGTGCTT
  721 ACCAGACCGG AGATATTGAC ATCATATATG CCTTGAGCAA CTGATAGCTG TCGCTGTCAA
  781 CTGTCACTGT AATACGCTGC TTCATAGCAT ACCTCTTTTT GACATACTTC GGGTATACAT
  841 ATCAGTATAT ATTCTTATAC CGCAAAAATC AGCGCGCAAA TACGCATACT GTTATCTGGC
  901 TTTTAGTAAG CCGGGATCCA CGCGTTTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT
  961 GTAATTTCAT TAAAGCATTC TGCCCGACAT GGAGCCCATC ACAAACGGGC ATGGATGGAT
 1021 CTGAATCGCC AGCGGCCATC AGCACATGAT CGGCTGGCGA TATAATTTTG CCAATGGCTG
1081 AAAACGGGGG CGAAAGCAG
//
Primer: EF1a-C_F RDB12872_A4Krc.seq
>12872_12872_A4Kr_2_EF1a-C_F_B02_05_ABI24.ab1
    1 CAGGGCATGA TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA
   61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCCACCAT GGTGAGCGTG
  121 ATCAAGCCCG AGATGAAGAT GAAGTACTTC ATGGACGGCA GCGTGAACGG CCACGAGTTC
  181 ACCGTGGAGG GCGAGGGCAC CGGCAAGCCC TACGAGGGCC ACCAGGAGAT GACCCTGAGG
  241 GTGACAATGG CCAAGGGCGG CCCCATGCCC TTCAGCTTCG ACCTGGTGAG CCACACCTTC
  301 TGCTACGGCC ACAGGCCCTT CACCAAGTAC CCCGAGGAGA TCCCCGACTA CTTCAAGCAG
  361 GCCTTCCCCG AGGGCCTGAG CTGGGAGAGG AGCCTCCAGT TCGAGGACGG CGGCTTCGCC
  421 GCCGTGAGCG CCCACATCAG CCTGAGGGGC AACTGCTTCG AGCACAAGAG CAAGTTCGTG
  481 GGCGTGAACT TCCCCGCCGA CGGCCCCGTG ATGCAGAACC AGAGCAGCGA CTGGGAGCCC
  541 AGCACCGAGA AGATCACCAC CTGCGACGGC GTGCTGAAGG GCGACGTGAC CATGTACCTG
  601 AAGCTGGCCG GCGGCGGCAA CCACAAGTGC CAGTTCAAGA CCACCTACAA GGCCGCCAAG
  661 AAGATCCTGA AGATGCCCCA GAGCCACTTC ATCGGCCACA GGCTGGTGAG GAAGACCGAG
  721 GGCAACATCA CCGAGCTGGT GGAGGACGCC GTGGCCCACT GCGAGGGCAG AGGAAGTCTT
  781 CTAACATGCG GTGACGTGGA GGAGAATCCC GGCCCTATGT CTAGACTGGA CAAGAGCAAA
  841 GTCATAAACG GCGCTCTGGG AATTTACTCA ATGGGAGTCG GTATCGAAAG GCCTGACGAC
  901 AAGGAAACTC GCTCAAAAGC TGGGGAGTTT GAGGCAGCCT ACCCTGTACT GGCACGTGAA
  961 GAACAAGCGG GCCTGCTCGA ATGCTCCTGC CAATCGAGAT GCTTGAACAG GCATCATACC
 1021 CACTCTGCCC CCTGGGAAGG CGAGTCATGC AAGGACTTCT GCGAACACGC CTGGTTCAGT
1081 TCCAGCTGTT GACGTCGAC
//
Primer: hKO_F RDB12872_A4Krd.seq
>12872_12872_A4Kr_2_hKO_F_A03_03_ABI24.ab1
    1 CCTATGGGGC ACAGGCTGGT GAGGAGACCG AGGGCAACAT CACCGAGCTG GTGGAGGACG
   61 CCGTGGCCCA CTGCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC
  121 CCGGCCCTAT GTCTAGACTG GACAAGAGCA AAGTCATAAA CGGCGCTCTG GAATTACTCA
  181 ATGGAGTCGG TATCGAAGGC CTGACGACAA GGAAACTCGC TCAAAAGCTG GGAGTTGAGC
  241 AGCCTACCCT GTACTGGCAC GTGAAGAACA AGCGGGCCCT GCTCGATGCC CTGCCAATCG
  301 AGATGCTGGA CAGGCATCAT ACCCACTTCT GCCCCCTGGA AGGCGAGTCA TGGCAAGACT
  361 TTCTGCGGAA CAACGCCAAG TCATTCCGCT GTGCTCTCCT CTCACATCGC GACGGGGCTA
  421 AAGTGCATCT CGGCACCCGC CCAACAGAGA AACAGTACGA AACCCTGGAA AATCAGCTCG
  481 CGTTCCTGTG TCAGCAAGGC TTCTCCCTGG AGAACGCACT GTACGCTCTG TCCGCCGTGG
  541 GCCACTTTAC ACTGGGCTGC GTATTGGAGG AACAGGAGCA TCAAGTAGCA AAAGAGGAAA
  601 GAGAGACACC TACCACCGAT TCTATGCCCC CACTTCTGAG ACAAGCAATT GAGCTGTTCG
  661 ACCGGCAGGG AGCCGAACCT GCCTTCCTTT TCGGCCTGGA ACTAATCATA TGTGGCCTGG
  721 AGAAACAGCT AAAGTGCGAA AGCGGCGGGC CGGCCGACGC CCTTGACGAT TTTGACTTAG
  781 ACATGCTCCC AGCCGATGCC CTTGACGACT TTGACCTTGA TATGCTGCCT GCTGACGCTC
  841 TTGACGATTT TGACCTTGAC ATGCTCCCCG GGTAACTAAG GTAAGGATCC TCTAGGAGTT
  901 AACATCGAGG GGATCAAGCT TTATCGATAA ATCAAACCTC TGGGATTAAC AAAAATTTGG
  961 TGAAAGATTG AACTGGGTAT TTCTTTAACT ATGTTGGCTT CCCTTTTACG GCTAATGTGG
 1021 GAATAACGCT GCTTAATGGC CTTATGGTAT TCATGCCTAT GCCTTCCGGG TATGGGCTTT
1081 CAATTATCTC CTTCATTGGA TAAATCTGAT TGGCGTGATT CCTCA
//
Primer: CMV-Forward RDB12872_A4Kre.seq
>12872_12872_A4Kr_2_CMV-Forward_B03_06_ABI24.ab1
    1 AACAACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GCGGCCGCTG
  781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAAATTGAAC CATTAGGAGT AGCACCCACC AAAGGCAAAG AGAAGAGTGG
  901 GTGCAGAGAG AAAAAAAGAG CAGTGGGAAT AGGAGCTTTT GTTCCTTGGG TTTCTTTGGG
  961 GAGCAGCAGG GAAAGCACTA TGGGCGCAGC GGTCAAATGG ACGGCTGACG GGTTACAGCC
1021 AAGACCATAA TGTCTGTATA GTTGCAGCCA GGCAGAACCA AATTTGGCCT GTGGAG
//
Primer: BGH_rev2 RDB12872_A4Krf.seq
>12872_12872_A4Kr_2_BGH_rev2_C03_09_ABI24.ab1
    1 CTATCCAGGA TAGTAGGCAC GGGGGAGGGG CAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGAAT AACTTCGTAT
  301 AATGTATGCT ATACGAAGTT ATGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA
  361 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC
  421 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC
  481 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA
  541 GGTCTCGACG GTATCGATGC GGGGAGGCGG CCCAAAGGGA GATCCGACTC GTCTGAGGGC
  601 GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG
  661 CTGGATTGAG GGCCGAAGGG ACGTAGCAGA AGGACGTCCC GCGCAGAATC CAGGTGGCAA
  721 CACAGGCGAG CAGCCATGGA AAGGACGTCA GCTTCCCCGA CAACACCACG GAATTGTCAG
  781 TGCCCAACAG CCGAGCCCCT GTCCAGCAGC GGGCAAAGGC AGGCGGCGAT GAGTTCCGCC
  841 GTGGCAATAG GGAGGGGGAA AGCGAAAGTC CCGGAAAGGA GCTGACAGGT GGTGGCAATG
  901 CCCCAACCAG TGGGGGGTTG CGTCAGCAAA CACAGTGCAC ACCACGCCAC GTTGCCTGAC
  961 AACGGCCACA ACTCCTCATA AAGAGACAGC AACCAGGATT TATACAGGAG GAGAAAATGA
 1021 AAGCCATACG GAAGCAATAG CATGAATACC AAAGGGCATA AGCAGCGTAT CCACTTAGCC
1081 GTTAAAGGAG CCAACAATAG TTCAGAATAA CCCAAGGTCA CAGATTCGCT
//
Primer: SV40pro_F RDB12872_A4Krg.seq
>12872_12872_A4Kr_2_SV40pro_F_D03_12_ABI24.ab1
    1 ACGGGCCCGT ACTTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA
  121 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG
  181 TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG
  241 ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG
  301 GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG
  361 GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC
  421 ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG
  481 CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG
  541 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
  601 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
  661 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
  721 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
  781 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
  841 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACAACAACAT
  901 ACGAGCCGGA AGCATAAAAG TGTAAAGCCC TGGGGGTGCC CTAATGAGTT GAGCTAAACT
  961 CACATTTAAA TTGCGTTTGC CGCTCACTGC CCGCTTTCCA GTTCGGGGAA AACCCTTGTT
 1021 CGTGCCAGCT TGCCATTAAT GAAATTCGGC TAACGCGTCG GTGCAGAAGC CGGTTGCCGT
1081 ATTTGGCGCT CTTCCGGCAT TCCTTCGACT TCACATGGAC AC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iida, T., Whole-Genome DNA Methylation Analyses Revealed Epigenetic Instability in Tumorigenic Human iPS Cell-Derived Neural Stem/Progenitor Cells. Stem Cells 35 (5): 1316-1327 (2017). PMID 28142229.
reference Kwon, S.J., Role of MEK partner-1 in cancer stemness through MEK/ERK pathway in cancerous neural stem cells, expressing EGFRviii. Mol. Cancer 16 (1): 140 (2017). PMID 28830458.

2018.10.09

GNP_filter3_RDBDEP_html_180823.pl