Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSIV-GFP-TRE-EF-KT (#RDB12871)

Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter.

Clone info. Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter. Please note that the TRE and EF promoters are placed in back-to-back orientation and this construct contains loxP.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSIV-RfA-TRE-EF-KT (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence (full) RDB12871zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12871 CSIV-GFP-TRE-EF-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-GFP-TRE-EF-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12871).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19477_B2Abp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: VN_f1 (Pr0992)
Region: EGFP 5',attB2,cPPT_CTS
Sequence file: RDB19477_B2Aba.seq check
>12871_19477_B2Ab_2_VN_F1_F01_06_ABI08.ab1
    1 GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA
   61 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
  121 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
  181 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT
  241 GGACGAGCTG TACAAGTCCG GACTCAGATC CTACTAGTAG GATCTCGAGA TATCTAGACC
  301 CAGCTTTCTT GTACAAAGTG GTGATGCTAC CGGAGAATTC AAAGTGAATT TCTGCTGTCC
  361 CTGTAATAAA CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT
  421 ATGTCTGTTG CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCNN NNNNNCCCCT
  481 TTTCTTTTAA AATTGTGGAT GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA
  541 TCTGTCTCTG TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCNGGCCTGT CGGGTCCCCT
  601 CGGGGTTGGG AGGTGGGTCT GAAAACNANN ANTGGTGAAT ATCCNTGNCN NNACTCTATT
661 NNNCNNTANA AANGTACNNG NNNAAAAACT NANTTNNNNN NNNCCNNNNC NNNNNN
//
Primer: EF1a-N_R (Pr0024)
Region: EF-1 alpha pro,tight TRE pro,attB1,EGFP 5'
Sequence file: RDB19477_B2Abb.seq check
>12871_19477_B2Ab_2_EF1a_N_R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCGNNGTG CGCTCTGCCC NCTGACGGGC ACCGGAGCCT
   61 CACGAAGCTT GAATTCGGCC CTTTCGTCTT CAATCGAGTT TACTCCCTAT CAGTGATAGA
  121 GAACGTATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC GATGTCGAGT TTACTCCCTA
  181 TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA
  241 GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ATCCCTATCA GTGATAGAGA
  301 ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGGT AGGCGTGTAC
  361 GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC GCTAGCATCA
  421 CAAGTTTGTA CAAAAAAGCA GGCTCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCNCCG
  481 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCNGCGTGT
  541 CCGGCGAGGG CGAGGGCGAT GCCNCCTACN GCAAGCTGAN CCTGAAGTTN NTCTGCACCA
  601 CCGGCNAGCT GCCCGNGCCC NNNNCCNCCC TCNNGACCNN CCTGACCTAC NGNGNGNANN
661 GNNTCNNCCG CTACCCNNAN CNCNTGAAGN NNNNNNANTT CNTCANNCNN NNNNNNNN
//
Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,hKO 5'
Sequence file: RDB19477_B2Abc.seq check
>12871_19477_B2Ab_2_EF1a-C_F_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TNAGTTTGGN NCTTGGTTNN TTCTCNANGC CTNNNACAGT
   61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAACA CGCTACCGGT CCACCATGGT
  121 GAGCGTGATC AAGCCCGAGA TGAAGATGAA GTACTTCATG GACGGCAGCG TGAACGGCCA
  181 CGAGTTCACC GTGGAGGGCG AGGGCACCGG CAAGCCCTAC GAGGGCCACC AGGAGATGAC
  241 CCTGAGGGTG ACAATGGCCA AGGGCGGCCC CATGCCCTTC AGCTTCGACC TGGTGAGCCA
  301 CACCTTCTGC TACGGCCACA GGCCCTTCAC CAAGTACCCC GAGGAGATCC CCGACTACTT
  361 CAAGCAGGCC TTCCCCGAGG GCCTGAGCTG GGAGAGGAGC CTCCAGTTCG AGGACGGCGG
  421 CTTCGCCGCC GTGAGCGCCC ACATCAGCCT GAGGGGCAAC TGCTTCGAGC ACAAGAGCAA
  481 GTTCGTGGGC GTGAACTTCC CCGCCGACGG CCCCGTGATG CAGAACCNGA GCAGCGACTG
  541 GGAGCCCAGC ACCNAGAAGA TCACCNCCTG CNACNGCGTG CTGAAGGGCG ANGTGACCAT
  601 GTACCTGAAN NTGGNCGGNG GNNGCANCCA CNAGTGCNNN TTCNANANCN NCTACNANNN
  661 NNNCAANAAN ANNCTGAANA NGNCCNNNAN CCNCTTCNTC NGNNNNNNGN NGGNNNNGAN
721 NANNNNGGGN NNNNNNCCNA NCNGNGNNNN NNNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE,rt TA Advanced
Sequence file: RDB19477_B2Abd.seq check
>12871_19477_B2Ab_2_PRE_rev_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNA AGGAGCANNN TAGTTAAGAA TACCAGTCAA TCTTTCNCAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG
  121 ATCCTTACTT AGTTACCCGG GGAGCATGTC AAGGTCAAAA TCGTCAAGAG CGTCAGCAGG
  181 CAGCATATCA AGGTCAAAGT CGTCAAGGGC ATCGGCTGGG AGCATGTCTA AGTCAAAATC
  241 GTCAAGGGCG TCGGCCGGCC CGCCGCTTTC GCACTTTAGC TGTTTCTCCA GGCCACATAT
  301 GATTAGTTCC AGGCCGAAAA GGAAGGCAGG TTCGGCTCCC TGCCGGTCGA ACAGCTCAAT
  361 TGCTTGTCTC AGAAGTGGGG GCATAGAATC GGTGGTAGGT GTCTCTCTTT CCTCTTTTGC
  421 TACTTGATGC TCCTGTTCCT CCAATACGCA GCCCAGTGTA AAGTGGCCCA CGGCGGACAG
  481 AGCGTACAGT GCGTTCTCCA GGGAGAAGCC TTGCTGACAC AGGAACGCGA GCTGATTTTC
  541 CNGGGTTTCG TACTGTTTCT CTGTTGGGCG GGTGCCGAGA TGCACTTTAG CCCCGTCNCG
  601 ATGTGAGAGG ANAGCACNGC NNAATGACNT GGCGTTGNTC CNNNNAAANN CNTGNCNNGA
  661 CTCNNNNNNC NNGGGGNNNA ANNGGNNANG ANGNCNGNNC NNNNTCTCNA NNNNNNNNNT
721 CNNNNNNNNN N
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,5' LTR,HIV-1 psi
Sequence file: RDB19477_B2Abe.seq check
>12871_19477_B2Ab_2_CMV_Forward_F06_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN TTGNNNCNNN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG
  121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC
  181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT
  241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG
  301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG
  361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG
  421 AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT
  481 TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG
  541 GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA
  601 AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA
  661 TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA
  721 AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC
  781 CGCTGATCTT CAGANCTGGA GGANGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATN
  841 AATATAAAGT AGTAAAAATT GAACCATTAN GAGTAGCACC CACCNAGGCA AANAGAAGAG
  901 TGGNTGCAGA NAGNAAAAAG ANCAGNGGGA ATANGANCTT TGTTCCNNGG GTNCTNGGGA
  961 GCAGCNNNAN NCACTATNGG CGCNNCGNNN NTGNNGCTNN NNNTNNNNNN NCNNNNNNNT
1021 ATNGNNNNNN NNNN
//
Primer: RRE_F1 (Pr0796)
Region: RRE
Sequence file: RDB19477_B2Abf.seq check
>12871_19477_B2Ab_2_RRE_F1_F02_17_ABI24.ab1
    1 NNNNNNNNNN NGNNNNNNNN NAANNATTTG CTGAGGGCTA TTGAGGCGCA ACAGCATCTG
   61 TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA
  121 TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC
  181 ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC
  241 ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA
  301 ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA
  361 TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC
  421 ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG
  481 AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCNGA CCCACCTCCC AACCCCGAGG
  541 GGACCCGACA GGCCCGAANG AATAGAAGAA GAANGTGGAG AGAGAGACAG ANACNGATCC
  601 NTTCNANTAG TGNACGGATC TACNAATGGC AGTATTNNTC NNCNANTTTN AAANAAAANG
  661 GGGGNNTGGG GGGNANNGNG NNNGGNAAAG ANAGTNNANN TNATANCANN GANNTNCNAN
721 CNNANNANTN NNAANNANTN NNANNNNNNT TNNNNNNNNN N
//
Primer: EGFP-M_R (Pr0743)
Region: EGFP 5',attB1
Sequence file: RDB19477_B2Abg.seq check
>12871_19477_B2Ab_2_EGFP-M_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG TGGNGGNNCT TGNNGTTNNC CTTGATGCCG TTCTTCTGCT
   61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
  121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
  181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
  241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
  301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG
  361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT
  421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
  481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GTGGAGCCTG
  541 CTTTTTTGTA CNAACTTGTG ATGCTAGCGG CGATCTGACG GTTCNCTAAA CGAGCTCTGC
  601 TNATATAGGC NTCCNANCGN ACACNNCTAC CTCNNCNTNC GTTCTCTATC ACTGATNGGG
  661 AGTAANCTCN ACNNNCNTNC TCTANNNCNG ANANGGANNA ANTCNANNTN NNTNNCNNTC
721 NNNGANNNGN NNTNAANNNN NNNNNN
//
Primer: tTA-Ad R (Pr0542)
Region: rt TA-Advanced 5',T2A,hKO 3'
Sequence file: RDB19477_B2Abh.seq check
>12871_19477_B2Ab_2_tTA-Ad R_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNGANTTTG CTCTTGTCNN GTCTAGNNCN TAGGGCCGGG
   61 ATTCTCCTCC ACGTCACCGC ATGTTAGAAG ACTTCCTCTG CCCTCGCAGT GGGCCACGGC
  121 GTCCTCCACC AGCTCGGTGA TGTTGCCCTC GGTCTTCCTC ACCAGCCTGT GGCCGATGAA
  181 GTGGCTCTGG GGCATCTTCA GGATCTTCTT GGCGGCCTTG TAGGTGGTCT TGAACTGGCA
  241 CTTGTGGTTG CCGCCGCCGG CCAGCTTCAG GTACATGGTC ACGTCGCCCT TCAGCACGCC
  301 GTCGCAGGTG GTGATCTTCT CGGTGCTGGG CTCCCAGTCG CTGCTCTGGT TCTGCATCAC
  361 GGGGCCGTCG GCGGGGAAGT TCACGCCCAC GAACTTGCTC TTGTGCTCGA AGCAGTTGCC
  421 CCTCAGGCTG ATGTGGGCGC TCACGGCGGC GAAGCCGCCG TCCTCGAACT GGAGGCTCCT
  481 CTCCCAGCTC AGGCCCTCGG GGAAGGCCTG CTTGAAGTAG TCGGGGATCT CCTCGGGGTA
  541 CTTGGTGAAG GGCCTGTGGC CGTAGCANAN NGTGTGGCTC ACCNGGTCNA AGCTNNANNG
  601 GCATGGGGNC GCCCTNGGNN NTTGTNNCCC TNNNGGTCNT CTCCTGGNGN NCNNCNTNNG
  661 NCTTGNCNGN GCCNTCNCCC NCCNNGGNGN ANTNNNNNNN NTNNNGNTNN NTNCNTNANN
721 NNNNNNNCNN NNNNNCNGNC NNGANNNNNN NNNN
//
Primer: BGH_rev3 (Pr0885)
Region: bGH pA,3' LTR,lox P
Sequence file: RDB19477_B2Abi.seq check
>12871_19477_B2Ab_2_bGH_rev3_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN GGNNGNNNNN NNNACNGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
  121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
  181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
  241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGAATAA CTTCGTATAA
  301 TGTATGCTAT ACGAAGTTAT GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT
  361 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT
  421 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG
  481 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCNTG TTTTTCTAGG
  541 TCTCGACGGT ATCGATGCGG GGAGGCGGNC CNNAGGGAGA TCCGACTCGT CTGANGGCGA
  601 ANGCNAANAC GCNGAANANG NCNCNNANCC NGCAGCNNNC NCGGGAANGN NGNNCCNCTG
661 GNNTGNNGGN NNANNGNANN TANNNNNNNN NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,Zeo
Sequence file: RDB19477_B2Abj.seq check
>12871_19477_B2Ab_2_SV40pro_ori_F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG CCTCNNNNNN ATTCNNGAAG TAGTGAGGAG
   61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
  121 GATCTGATCA GCACGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA
  181 CGACAAGGTG AGGAACTAAA CCATGGCCAA GTTGACCAGT GCCGTTCCGG TGCTCACCGC
  241 GCGCGACGTC GCCGGAGCGG TCGAGTTCTG GACCGACCGG CTCGGGTTCT CCCGGGACNT
  301 CNTGGAGGAC GACTTCGCCG GTGTGGTCCG GGACGACGTG ACCCTGTTCA TCAGCGCGGT
  361 CCAGGACCAG GTGGTGCCGG ACAACACCCT GGCCTGGGTG TGGGTGCGCG GCCTGNACGA
  421 GCTGTACNCC NAGTGGTCGG AGGTCGTGNN NNNNAACTTC CGGGACGCCT CCGGGCCNGN
  481 NNNGACCGAG ATCGGCNAGN NNCCGTGGGG GCGGGAGTTC GCCCTGCNCG ACCCGGCCGG
  541 CAACTGNNNG CACTTCGTGG NCNANGANCA NGANTGACNC NNNNNNNNAG ATTTCNATNC
  601 CNCCNNCNCC TTCTATGAAN NGNTNGGNCT TNNNAATCNN TNNNCNGNNN NNNNGNNGGN
  661 NGANNCNNCN NNNNGNNNAT NNNNNNNNGN NNNTNNNNNN CCNNCCNNNC NNNNNNANNN
721 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles