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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSIV-GFP-TRE-EF-KT

Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter.

Catalog number RDB12871
Resource name CSIV-GFP-TRE-EF-KT
Clone info. Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter. Please note that the TRE and EF promoters are placed in back-to-back orientation and this construct contains loxP.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-RfA-TRE-EF-KT (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering Please visit Ordering instruction.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Schedule A (GFP-A) [Link]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12871 CSIV-GFP-TRE-EF-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15988_A8Ffp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-N_R
Sequence file: RDB15988_A8Ffa.seq check
>12871_15988_A8Ff_EF1a-N_R_A01_01_ABI08.ab1
    1 TCCTTTCTCC GGGGGGACTG GTGGGGGCGA TGTGCGCTCT GCCCACTGAC GGGGCACCGG
   61 AGCCTCACGA AGCTTGAATC TCGGCCCTTT CGTCTTCAAT CGAGTTTACT CCCTATCAGT
  121 GATAGAGAAC GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC
  181 TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA
  241 TGTCGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA
  301 TAGAGAACGT ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGGTAGGC
  361 GTGTACGGTG GGAGGCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCGCTA
  421 GCATCACAAG TTTGTACAAA AAAGCAGGCT CCACCATGGT GAGCAAGGGC GAGGAGCTGT
  481 TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA
  541 GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT
  601 GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG
  661 TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA
  721 TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA
  781 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGGA GCTGAAAGGG
  841 CATCGACTTC AAGGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA
  901 GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG GCATCAAGGT TGAACTTCAA
  961 GATCCGCCAC AAACATCGAA GGACGGGCAG CGTGCAGCTT CGGCCGACCA CTTACCAGGC
 1021 AAGAACAACC CCCCATTCGG CCGACGGCCC CGTGCTTGCT TGCCCGAACA ACCACTTACC
 1081 CTGAGCACCC AGTTCCCGTC CCTGAAGTAA GAACCCTCAA CCGAGAAGGC GCGGAATCCA
1141 CATTGGTTCC CCTTGGACCT TGTGGA
//
Primer: cPPT+CTS_v2_F
Sequence file: RDB15988_A8Ffb.seq check
>12871_15988_A8Ff_cPPT+CTS_V2_F_C01_03_ABI08.ab1
    1 GCGAGGAAAA TTCCAAACCT AAAGAAATTA CAAAAACAAA TTACAAAAAA ATTCAAAATT
   61 TTCGGGTTTA TTACAGGGAC AGCAGTAAAT TCACTTTGAA TTCTCCGGTA GCATCACCAC
  121 TTTGTACAAG AAAGCTGGGT CTAGATATCT CGAGATCCTA CTAGTAGGAT CTGAGTCCGG
  181 ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA
  241 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGGTG CTCAGGTAGT
  301 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG
  361 CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTCACC TTGATGCCGT
  421 TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT
  481 GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA
  541 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA
  601 AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT
  661 TCATGTGGTC GGGGTAGCGG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG
  721 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCC
  781 GTAGGTGGCA TCGCCCTCGC CCTCGCCGGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC
  841 CGTCCAGCTC GAACCAGGAT GGGCACCACC CCGGTGAACA AGCTCCTCGG CCCTTGCTCA
  901 CCATGGGTGG GAGCCCTGCT TTTTTGTACA AACCTTGTGA TGGCTAAGCG GCGATCTGAC
  961 GGTTTCACTA AAACGAAGCT TCTTGCTTTA TATAAGCCCT CCCACCCGGT AACACGCCCT
 1021 ACCCTCCGAA CATAACGTTT CTTCTATCAA CTGATTAGGG GAGTTAAACT TCGACCATAC
 1081 CGGTTTCCTC CTATCCACTT GAATAAGGGG ATTAAAACTT CGGAACAATA CGGTTTCTTC
 1141 TCTAATCCAC TGAATGGGGA AGTTAAACTT CGGGACCAAT ATACGATTCT CTAATTCAAC
1201 CTTGAAATAA GCGGGCACAG
//
Primer: EF1a-C_F
Sequence file: RDB15988_A8Ffc.seq check
>12871_15988_A8Ff_EF1a-C_F_E01_05_ABI08.ab1
    1 TTTGTGGAAA TGGGCCTTTT TGGAGTTTGG GATCTTGGTT CATTCTCAAG CCTCAGACAG
   61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCCACCATGG
  121 TGAGCGTGAT CAAGCCCGAG ATGAAGATGA AGTACTTCAT GGACGGCAGC GTGAACGGCC
  181 ACGAGTTCAC CGTGGAGGGC GAGGGCACCG GCAAGCCCTA CGAGGGCCAC CAGGAGATGA
  241 CCCTGAGGGT GACAATGGCC AAGGGCGGCC CCATGCCCTT CAGCTTCGAC CTGGTGAGCC
  301 ACACCTTCTG CTACGGCCAC AGGCCCTTCA CCAAGTACCC CGAGGAGATC CCCGACTACT
  361 TCAAGCAGGC CTTCCCCGAG GGCCTGAGCT GGGAGAGGAG CCTCCAGTTC GAGGACGGCG
  421 GCTTCGCCGC CGTGAGCGCC CACATCAGCC TGAGGGGCAA CTGCTTCGAG CACAAGAGCA
  481 AGTTCGTGGG CGTGAACTTC CCCGCCGACG GCCCCGTGAT GCAGAACCAG AGCAGCGACT
  541 GGGAGCCCAG CACCGAGAAG ATCACCACCT GCGACGGCGT GCTGAAGGGC GACGTGACCA
  601 TGTACCTGAA GCTGGCCGGC GGCGGCAACC ACAAGTGCCA GTTCAAGACC ACCTACAAGG
  661 CCGCCAAGAA GATCCTGAAG ATGCCCCAGA GCCACTTCAT CGGCCACAGG CTGGTGAGGA
  721 AGACCGAGGG CAACAATCAC CGAGCTGGTG GAGGACGCCG TGGCCCACTG CGAGGGCAGA
  781 GGAAGTCTTC TAACATGCGG TGACGTGGAG GAGAATCCCG GCCCTATGTC TAGACTGGAC
  841 AAGAGCAAAG TCATAAAACG GCGCTCTGGA ATTACTCAAT GGAGTCGGTA TCGAAGGCCT
  901 GACGACAAGG GAAACTCGCT CAAAAGCTGG GAGGTTGAGC AGCCTACCCT GTACTGGCAC
  961 GTGAAGAAAC AAGCGGGCCC TGCTTCGATG CCCTGCCCAA TCGAGATGCT GGGACAGGGC
 1021 ATCATACCCA ACTTCCTGGC CCCCTGGGAA AGGCCGAGGT CCATGGCAAG ACTTTTCTGG
1081 CGGAACACGC TAGGTCCATC GGCCTGTGCT CTCTTCTCAC ATCGCGGAAA CGGGGGG
//
Primer: hKO_F
Sequence file: RDB15988_A8Ffd.seq check
>12871_15988_A8Ff_hKO_F_G01_07_ABI08.ab1
    1 ACCATGGGGG CAAGGGCTGG TGAGGAGGAC CGAGGGCAAC ATCACCGAGC TGGTGGAGGA
   61 CGCCGTGGCC CACTGCGAGG GCAGAGGAAG TCTTCTAACA TGCGGTGACG TGGAGGAGAA
  121 TCCCGGCCCT ATGTCTAGAC TGGACAAGAG CAAAGTCATA AACGGCGCTC TGGAATTACT
  181 CAATGGAGTC GGTATCGAAG GCCTGACGAC AAGGAAACTC GCTCAAAAGC TGGGAGTTGA
  241 GCAGCCTACC CTGTACTGGC ACGTGAAGAA CAAGCGGGCC CTGCTCGATG CCCTGCCAAT
301 CGAGATGCTG GACAGGCATC ATACCCACTT CTGCCCCCTG G
//
Primer: CMV-Forward
Sequence file: RDB15988_A8Ffe.seq check
>12871_15988_A8Ff_CMV-Forward_D02_11_ABI24.ab1
    1 TTCACCATCG GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
  721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGCGGCCGCT
  781 GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA
  841 TAAAGTAGTA AAAAATTGAA CCATTAGGAG TAGCACCCAC CAAGGCAAAG AGAAGAGTGG
  901 TGCAGAGAGA AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA
  961 GCAGGAAAGC ACTATGGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC AGACATTTAT
 1021 TGTCTGGTAT AGTTGCAGCA GCAGACAATT TGGCCGTGAG GGCCTTATTT GAGGCCGGCC
1081 AAACAAGGTC ATCCTGAATG
//
Primer: BGH_rev2
Sequence file: RDB15988_A8Fff.seq check
>12871_15988_A8Ff_BGH_rev2_E02_14_ABI24.ab1
    1 TCGGGTAAGG AAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
  121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
  181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
  241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGAATAACT TCGTATAATG
  301 TATGCTATAC GAAGTTATGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
  361 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
  421 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
  481 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
  541 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
  601 GCGAAGACGC GGAAGAGGCC GCTGGAGCCG GCAGCAGGCC GCGGGAAGGA AGGTCCGCTG
  661 GATTGAGGGC CGAAGGGACG TAGCAGAAGG ACGTCCCGCG CAGAATCCAG GTGGCAACAC
  721 AGGCGAGCAG CCATGGAAAG GACGTCAGCT TCCCCGACAA CACCACGGAA TTGTCAGTGC
  781 CCAACAGCCG AGCCCCTGTC CAGCAGCGGG CAAGGCAGGC GGCGATGAGT TCCGCCGTGG
  841 CAATAGGGAG GGGGAAAGCG AAAGTCCCGG AAAGGAGCTG ACAGGTGGTG GCAATGCCCC
  901 AACCAGTGGG GGGTTTGCGT CAGCAAACAC AGTGCACACC ACGCCACGTT TGCCCTGACA
  961 ACGGGCCACA ACTCCTCATA AAGAGACAGC AACCAGGATT TATACAAGGA GGAGAAAATG
 1021 AAAGCCATAC GGGAAAGCAA TAGCATGATT ACAAAGGGCA TTAAAAGCAG CGTTATTCCA
1081 ACAATAGCCG TAAAAACGGA GCACCAATTA GTTTAAGAAA TATACCAAGG
//
Primer: SV40pro_F
Sequence file: RDB15988_A8Ffg.seq check
>12871_15988_A8Ff_SV40pro_F_F02_17_ABI24.ab1
    1 CCTGGGTTCG GGGCATTCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
  121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
  181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
  241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
  301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
  361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
  421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
  481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
  541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
  661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
  721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
  841 CGTAATCATG GTTCATAGCT GGTTTCCTGG TGGTGAAATT GTTATTCCGC TCCACAATTT
  901 CCACACAACA TACGGAGCCG GAAAGCATAA AGTGGTAAGG GCTGGGGGTG CTAATGGAGG
  961 TGGAGCTAAC TCCACATTAT GGCGTGCGGC CTCCACTGGG CCGGCTTCAG TCGGAACTGG
 1021 TCTTGCCAGC TTGCAATAAT GATCGGCCAA GCCGGGAAGG CCGGGTTGCA TGGCCTCGTA
1081 TCTCGATTCA CTGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.12.24

GNP_filter3_RDBDEP_html_201222.pl