RDB12871 - CSIV-GFP-TRE-EF-KT

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CSIV-GFP-TRE-EF-KT (#RDB12871)

Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter.

Clone info.	Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter. Please note that the TRE and EF promoters are placed in back-to-back orientation and this construct contains loxP.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CSIV-RfA-TRE-EF-KT (Plasmid)
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence (full)	RDB12871zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12871	CSIV-GFP-TRE-EF-KT	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-GFP-TRE-EF-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12871).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19477_B2Abp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: VN_f1 (Pr0992) Region: EGFP 5',attB2,cPPT_CTS Sequence file: RDB19477_B2Aba.seq
>12871_19477_B2Ab_2_VN_F1_F01_06_ABI08.ab1 1 GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA 61 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT 121 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA 181 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT 241 GGACGAGCTG TACAAGTCCG GACTCAGATC CTACTAGTAG GATCTCGAGA TATCTAGACC 301 CAGCTTTCTT GTACAAAGTG GTGATGCTAC CGGAGAATTC AAAGTGAATT TCTGCTGTCC 361 CTGTAATAAA CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT 421 ATGTCTGTTG CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCNN NNNNNCCCCT 481 TTTCTTTTAA AATTGTGGAT GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA 541 TCTGTCTCTG TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCNGGCCTGT CGGGTCCCCT 601 CGGGGTTGGG AGGTGGGTCT GAAAACNANN ANTGGTGAAT ATCCNTGNCN NNACTCTATT 661 NNNCNNTANA AANGTACNNG NNNAAAAACT NANTTNNNNN NNNCCNNNNC NNNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,tight TRE pro,attB1,EGFP 5' Sequence file: RDB19477_B2Abb.seq
>12871_19477_B2Ab_2_EF1a_N_R_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGCGNNGTG CGCTCTGCCC NCTGACGGGC ACCGGAGCCT 61 CACGAAGCTT GAATTCGGCC CTTTCGTCTT CAATCGAGTT TACTCCCTAT CAGTGATAGA 121 GAACGTATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC GATGTCGAGT TTACTCCCTA 181 TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA 241 GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ATCCCTATCA GTGATAGAGA 301 ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGGT AGGCGTGTAC 361 GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC GCTAGCATCA 421 CAAGTTTGTA CAAAAAAGCA GGCTCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCNCCG 481 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCNGCGTGT 541 CCGGCGAGGG CGAGGGCGAT GCCNCCTACN GCAAGCTGAN CCTGAAGTTN NTCTGCACCA 601 CCGGCNAGCT GCCCGNGCCC NNNNCCNCCC TCNNGACCNN CCTGACCTAC NGNGNGNANN 661 GNNTCNNCCG CTACCCNNAN CNCNTGAAGN NNNNNNANTT CNTCANNCNN NNNNNNNN //
Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,hKO 5' Sequence file: RDB19477_B2Abc.seq
>12871_19477_B2Ab_2_EF1a-C_F_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN TNAGTTTGGN NCTTGGTTNN TTCTCNANGC CTNNNACAGT 61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAACA CGCTACCGGT CCACCATGGT 121 GAGCGTGATC AAGCCCGAGA TGAAGATGAA GTACTTCATG GACGGCAGCG TGAACGGCCA 181 CGAGTTCACC GTGGAGGGCG AGGGCACCGG CAAGCCCTAC GAGGGCCACC AGGAGATGAC 241 CCTGAGGGTG ACAATGGCCA AGGGCGGCCC CATGCCCTTC AGCTTCGACC TGGTGAGCCA 301 CACCTTCTGC TACGGCCACA GGCCCTTCAC CAAGTACCCC GAGGAGATCC CCGACTACTT 361 CAAGCAGGCC TTCCCCGAGG GCCTGAGCTG GGAGAGGAGC CTCCAGTTCG AGGACGGCGG 421 CTTCGCCGCC GTGAGCGCCC ACATCAGCCT GAGGGGCAAC TGCTTCGAGC ACAAGAGCAA 481 GTTCGTGGGC GTGAACTTCC CCGCCGACGG CCCCGTGATG CAGAACCNGA GCAGCGACTG 541 GGAGCCCAGC ACCNAGAAGA TCACCNCCTG CNACNGCGTG CTGAAGGGCG ANGTGACCAT 601 GTACCTGAAN NTGGNCGGNG GNNGCANCCA CNAGTGCNNN TTCNANANCN NCTACNANNN 661 NNNCAANAAN ANNCTGAANA NGNCCNNNAN CCNCTTCNTC NGNNNNNNGN NGGNNNNGAN 721 NANNNNGGGN NNNNNNCCNA NCNGNGNNNN NNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,rt TA Advanced Sequence file: RDB19477_B2Abd.seq
>12871_19477_B2Ab_2_PRE_rev_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNA AGGAGCANNN TAGTTAAGAA TACCAGTCAA TCTTTCNCAA 61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG 121 ATCCTTACTT AGTTACCCGG GGAGCATGTC AAGGTCAAAA TCGTCAAGAG CGTCAGCAGG 181 CAGCATATCA AGGTCAAAGT CGTCAAGGGC ATCGGCTGGG AGCATGTCTA AGTCAAAATC 241 GTCAAGGGCG TCGGCCGGCC CGCCGCTTTC GCACTTTAGC TGTTTCTCCA GGCCACATAT 301 GATTAGTTCC AGGCCGAAAA GGAAGGCAGG TTCGGCTCCC TGCCGGTCGA ACAGCTCAAT 361 TGCTTGTCTC AGAAGTGGGG GCATAGAATC GGTGGTAGGT GTCTCTCTTT CCTCTTTTGC 421 TACTTGATGC TCCTGTTCCT CCAATACGCA GCCCAGTGTA AAGTGGCCCA CGGCGGACAG 481 AGCGTACAGT GCGTTCTCCA GGGAGAAGCC TTGCTGACAC AGGAACGCGA GCTGATTTTC 541 CNGGGTTTCG TACTGTTTCT CTGTTGGGCG GGTGCCGAGA TGCACTTTAG CCCCGTCNCG 601 ATGTGAGAGG ANAGCACNGC NNAATGACNT GGCGTTGNTC CNNNNAAANN CNTGNCNNGA 661 CTCNNNNNNC NNGGGGNNNA ANNGGNNANG ANGNCNGNNC NNNNTCTCNA NNNNNNNNNT 721 CNNNNNNNNN N //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB19477_B2Abe.seq
>12871_19477_B2Ab_2_CMV_Forward_F06_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN TTGNNNCNNN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA 61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG 121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC 181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT 241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG 301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG 361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG 421 AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT 481 TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG 541 GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA 601 AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA 661 TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA 721 AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC 781 CGCTGATCTT CAGANCTGGA GGANGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATN 841 AATATAAAGT AGTAAAAATT GAACCATTAN GAGTAGCACC CACCNAGGCA AANAGAAGAG 901 TGGNTGCAGA NAGNAAAAAG ANCAGNGGGA ATANGANCTT TGTTCCNNGG GTNCTNGGGA 961 GCAGCNNNAN NCACTATNGG CGCNNCGNNN NTGNNGCTNN NNNTNNNNNN NCNNNNNNNT 1021 ATNGNNNNNN NNNN //
Primer: RRE_F1 (Pr0796) Region: RRE Sequence file: RDB19477_B2Abf.seq
>12871_19477_B2Ab_2_RRE_F1_F02_17_ABI24.ab1 1 NNNNNNNNNN NGNNNNNNNN NAANNATTTG CTGAGGGCTA TTGAGGCGCA ACAGCATCTG 61 TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA 121 TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC 181 ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC 241 ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA 301 ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA 361 TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC 421 ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG 481 AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCNGA CCCACCTCCC AACCCCGAGG 541 GGACCCGACA GGCCCGAANG AATAGAAGAA GAANGTGGAG AGAGAGACAG ANACNGATCC 601 NTTCNANTAG TGNACGGATC TACNAATGGC AGTATTNNTC NNCNANTTTN AAANAAAANG 661 GGGGNNTGGG GGGNANNGNG NNNGGNAAAG ANAGTNNANN TNATANCANN GANNTNCNAN 721 CNNANNANTN NNAANNANTN NNANNNNNNT TNNNNNNNNN N //
Primer: EGFP-M_R (Pr0743) Region: EGFP 5',attB1 Sequence file: RDB19477_B2Abg.seq
>12871_19477_B2Ab_2_EGFP-M_R_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG TGGNGGNNCT TGNNGTTNNC CTTGATGCCG TTCTTCTGCT 61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA 121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC 181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC 241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT 301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG 361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT 421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA 481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GTGGAGCCTG 541 CTTTTTTGTA CNAACTTGTG ATGCTAGCGG CGATCTGACG GTTCNCTAAA CGAGCTCTGC 601 TNATATAGGC NTCCNANCGN ACACNNCTAC CTCNNCNTNC GTTCTCTATC ACTGATNGGG 661 AGTAANCTCN ACNNNCNTNC TCTANNNCNG ANANGGANNA ANTCNANNTN NNTNNCNNTC 721 NNNGANNNGN NNTNAANNNN NNNNNN //
Primer: tTA-Ad R (Pr0542) Region: rt TA-Advanced 5',T2A,hKO 3' Sequence file: RDB19477_B2Abh.seq
>12871_19477_B2Ab_2_tTA-Ad R_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNT NNNGANTTTG CTCTTGTCNN GTCTAGNNCN TAGGGCCGGG 61 ATTCTCCTCC ACGTCACCGC ATGTTAGAAG ACTTCCTCTG CCCTCGCAGT GGGCCACGGC 121 GTCCTCCACC AGCTCGGTGA TGTTGCCCTC GGTCTTCCTC ACCAGCCTGT GGCCGATGAA 181 GTGGCTCTGG GGCATCTTCA GGATCTTCTT GGCGGCCTTG TAGGTGGTCT TGAACTGGCA 241 CTTGTGGTTG CCGCCGCCGG CCAGCTTCAG GTACATGGTC ACGTCGCCCT TCAGCACGCC 301 GTCGCAGGTG GTGATCTTCT CGGTGCTGGG CTCCCAGTCG CTGCTCTGGT TCTGCATCAC 361 GGGGCCGTCG GCGGGGAAGT TCACGCCCAC GAACTTGCTC TTGTGCTCGA AGCAGTTGCC 421 CCTCAGGCTG ATGTGGGCGC TCACGGCGGC GAAGCCGCCG TCCTCGAACT GGAGGCTCCT 481 CTCCCAGCTC AGGCCCTCGG GGAAGGCCTG CTTGAAGTAG TCGGGGATCT CCTCGGGGTA 541 CTTGGTGAAG GGCCTGTGGC CGTAGCANAN NGTGTGGCTC ACCNGGTCNA AGCTNNANNG 601 GCATGGGGNC GCCCTNGGNN NTTGTNNCCC TNNNGGTCNT CTCCTGGNGN NCNNCNTNNG 661 NCTTGNCNGN GCCNTCNCCC NCCNNGGNGN ANTNNNNNNN NTNNNGNTNN NTNCNTNANN 721 NNNNNNNCNN NNNNNCNGNC NNGANNNNNN NNNN //
Primer: BGH_rev3 (Pr0885) Region: bGH pA,3' LTR,lox P Sequence file: RDB19477_B2Abi.seq
>12871_19477_B2Ab_2_bGH_rev3_H02_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNN GGNNGNNNNN NNNACNGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA 121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG 181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC 241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGAATAA CTTCGTATAA 301 TGTATGCTAT ACGAAGTTAT GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT 361 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT 421 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG 481 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCNTG TTTTTCTAGG 541 TCTCGACGGT ATCGATGCGG GGAGGCGGNC CNNAGGGAGA TCCGACTCGT CTGANGGCGA 601 ANGCNAANAC GCNGAANANG NCNCNNANCC NGCAGCNNNC NCGGGAANGN NGNNCCNCTG 661 GNNTGNNGGN NNANNGNANN TANNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo Sequence file: RDB19477_B2Abj.seq
>12871_19477_B2Ab_2_SV40pro_ori_F_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG CCTCNNNNNN ATTCNNGAAG TAGTGAGGAG 61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 121 GATCTGATCA GCACGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA 181 CGACAAGGTG AGGAACTAAA CCATGGCCAA GTTGACCAGT GCCGTTCCGG TGCTCACCGC 241 GCGCGACGTC GCCGGAGCGG TCGAGTTCTG GACCGACCGG CTCGGGTTCT CCCGGGACNT 301 CNTGGAGGAC GACTTCGCCG GTGTGGTCCG GGACGACGTG ACCCTGTTCA TCAGCGCGGT 361 CCAGGACCAG GTGGTGCCGG ACAACACCCT GGCCTGGGTG TGGGTGCGCG GCCTGNACGA 421 GCTGTACNCC NAGTGGTCGG AGGTCGTGNN NNNNAACTTC CGGGACGCCT CCGGGCCNGN 481 NNNGACCGAG ATCGGCNAGN NNCCGTGGGG GCGGGAGTTC GCCCTGCNCG ACCCGGCCGG 541 CAACTGNNNG CACTTCGTGG NCNANGANCA NGANTGACNC NNNNNNNNAG ATTTCNATNC 601 CNCCNNCNCC TTCTATGAAN NGNTNGGNCT TNNNAATCNN TNNNCNGNNN NNNNGNNGGN 661 NGANNCNNCN NNNNGNNNAT NNNNNNNNGN NNNTNNNNNN CCNNCCNNNC NNNNNNANNN 721 NNNNNNN //

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