Resource data sheet
Prev. |  Lentiviral vector plasmid > 

CSIV-GFP-TRE-EF-KT

Lentivirus vector construct of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter.

Catalog number RDB12871
Resource name CSIV-GFP-TRE-EF-KT
Clone info. Lentivirus vector construct of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter. Please note that the TRE and EF promoters are placed in back-to-back orientation and this construct contains loxP.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-RfA-TRE-EF-KT (Plasmid)
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12871 CSIV-GFP-TRE-EF-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12871_A4J9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-N_R RDB12871_A4J9a.seq
>12871_12871_A4J9_1_EF1a-N_R_G01_19_ABI24.ab1
    1 CCTTTCCCGG GGGGGATGTG GGGCGATGTG CGCTCTGCCC ACTGACGGGC ACCGGAGCCT
   61 CACGAAGCTT GAATTCGGCC CTTTCGTCTT CAATCGAGTT TACTCCCTAT CAGTGATAGA
  121 GAACGTATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC GATGTCGAGT TTACTCCCTA
  181 TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA
  241 GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ATCCCTATCA GTGATAGAGA
  301 ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGGT AGGCGTGTAC
  361 GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC GCTAGCATCA
  421 CAAGTTTGTA CAAAAAAGCA GGCTCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
  481 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
  541 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA
  601 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT
  661 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
  721 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
  781 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
  841 TCAAGGAGGA CGGCAACATC CTGGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
  901 GGTCTATATC ATGGGCCCGA CAAGCAGAAG AACGGCATCA AAGTGAACTT CAAGATCCGC
  961 CACAACATCG AGGGACGGCA GCGTGCAGCT CGCCGACCAC TTACCAGCAG AACACCCCAT
 1021 CGGCGACGGC CCCCGTGCCT GGCTGGCCGG ACAACCAACT AACTGAATAA CCAGTCCGCC
1081 CTGGAGCAAG GAACT
//
Primer: cPPT+CTS_v2_F RDB12871_A4J9b.seq
>12871_12871_A4J9_1_cPPT+CTS_v2_F_F01_16_ABI24.ab1
    1 CATAAATACA AACTAAAGAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCGGGTTTA
   61 TTACAGGGAC AGCAGAAATT CACTTTGAAT TCTCCGGTAG CATCACCACT TTGTACAAGA
  121 AAGCTGGGTC TAGATATCTC GAGATCCTAC TAGTAGGATC TGAGTCCGGA CTTGTACAGC
  181 TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG
  241 CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG GACTGGGTGC TCAGGTAGTG GTTGTCGGGC
  301 AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG
  361 CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG
  421 TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG
  481 TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC
  541 TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC
  601 TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG
  661 GGGTAGCGGC TGAAGCACTG CACGCCGTAG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA
  721 CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC
  781 CCTCGCCCTC GCCCGGACAC GCTGAACTTG TGGGCCGTTT ACGTCGCCCG TCCAGCTCGA
  841 CCAGGATGGG CACCCACCCC CGGGTGAACA GCTCCTCCGC CCTTGCTCAC CATGGGTGGG
  901 AGCCCTGCTT TTTTTTGTAC AAACTTTGTG ATGCTAGGCG GGCGATCTGA CGGGTTCACT
  961 AAACGAGCCT TCTGCTTAAT AATAGCCTCC CCCACCGTAC ACGGCCTACC TCGAACAATA
 1021 CGGTTCTCTA TCACTGATAC GGGAGTTAAC TCGACAATAC CGGTTCCCTC CTAGTCAACG
//
Primer: EF1a-C_F RDB12871_A4J9c.seq
>12871_12871_A4J9_1_EF1a-C_F_A02_02_ABI24.ab1
    1 CAGTGAATGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AACACGCTAC CGGTCCACCA TGGTGAGCGT
  121 GATCAAGCCC GAGATGAAGA TGAAGTACTT CATGGACGGC AGCGTGAACG GCCACGAGTT
  181 CACCGTGGAG GGCGAGGGCA CCGGCAAGCC CTACGAGGGC CACCAGGAGA TGACCCTGAG
  241 GGTGACAATG GCCAAGGGCG GCCCCATGCC CTTCAGCTTC GACCTGGTGA GCCACACCTT
  301 CTGCTACGGC CACAGGCCCT TCACCAAGTA CCCCGAGGAG ATCCCCGACT ACTTCAAGCA
  361 GGCCTTCCCC GAGGGCCTGA GCTGGGAGAG GAGCCTCCAG TTCGAGGACG GCGGCTTCGC
  421 CGCCGTGAGC GCCCACATCA GCCTGAGGGG CAACTGCTTC GAGCACAAGA GCAAGTTCGT
  481 GGGCGTGAAC TTCCCCGCCG ACGGCCCCGT GATGCAGAAC CAGAGCAGCG ACTGGGAGCC
  541 CAGCACCGAG AAGATCACCA CCTGCGACGG CGTGCTGAAG GGCGACGTGA CCATGTACCT
  601 GAAGCTGGCC GGCGGCGGCA ACCACAAGTG CCAGTTCAAG ACCACCTACA AGGCCGCCAA
  661 GAAGATCCTG AAGATGCCCC AGAGCCACTT CATCGGCCAC AGGCTGGTGA GGAAGACCGA
  721 GGGCAACATC ACCGAGCTGG TGGAGGACGC CGTGGCCCAC TGCGAGGGCA GAGGAAGTCT
  781 TCTAACATGC GGTGACGTGG AGGAGAATCC CGGCCCTATG TCTAGACTGG ACAAGAGCAA
  841 AGTCATAAAC GGCGCTCTGG AATTACTCAA TGGAGTCGGT ATCGAAAGCC TGACGACAAA
  901 GGAAACTCGC TCAAAAGCTG GGGAGTTGAG CAGCCCTACT CCTGTACTGG CACGTGAAAG
  961 AACAAGCGGC TCCTTGCTTC GATGCTCTGC CCAATCGGAG ATGCTGGGAC AGGGCATCAT
 1021 TACCCAACTT TCTGCCCTCC TGGGAAGCCG AAGTCATGGG CAGACTTTCT GCGGACTACG
1081 GTCTAAGGTC ATTCCGCCTG TGTGAAT
//
Primer: hKO_F RDB12871_A4J9d.seq
>12871_12871_A4J9_1_hKO_F_B02_05_ABI24.ab1
    1 ATAACGGGGC CAAGGCTGGT GAGGAGACCG AGGGCAACAT CACCGAGCTG GTGGAGGACG
   61 CCGTGGCCCA CTGCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC
  121 CCGGCCCTAT GTCTAGACTG GACAAGAGCA AAGTCATAAA CGGCGCTCTG GAATTACTCA
  181 ATGGAGTCGG TATCGAAGGC CTGACGACAA GGAAACTCGC TCAAAAGCTG GGAGTTGAGC
  241 AGCCTACCCT GTACTGGCAC GTGAAGAACA AGCGGGCCCT GCTCGATGCC CTGCCAATCG
  301 AGATGCTGGA CAGGCATCAT ACCCACTTCT GCCCCCTGGA AGGCGAGTCA TGGCAAGACT
  361 TTCTGCGGAA CAACGCCAAG TCATTCCGCT GTGCTCTCCT CTCACATCGC GACGGGGCTA
  421 AAGTGCATCT CGGCACCCGC CCAACAGAGA AACAGTACGA AACCCTGGAA AATCAGCTCG
  481 CGTTCCTGTG TCAGCAAGGC TTCTCCCTGG AGAACGCACT GTACGCTCTG TCCGCCGTGG
  541 GCCACTTTAC ACTGGGCTGC GTATTGGAGG AACAGGAGCA TCAAGTAGCA AAAGAGGAAA
  601 GAGAGACACC TACCACCGAT TCTATGCCCC CACTTCTGAG ACAAGCAATT GAGCTGTTCG
  661 ACCGGCAGGG AGCCGAACCT GCCTTCCTTT TCGGCCTGGA ACTAATCATA TGTGGCCTGG
  721 AGAAACAGCT AAAGTGCGAA AGCGGCGGGC CGGCCGACGC CCTTGACGAT TTTGACTTAG
  781 ACATGCTCCC AGCCGATGCC CTTGACGACT TTGACCTTGA TATGCTGCCT GCTGACGCTC
  841 TTGACGATTT TGACCTTGAC ATGCTCCCCG GGTAACTAAG TAAGGATCCT CTAGAGTTTA
  901 ACATCGAGGG ATCAAGCTTT ATCGATAATC AACCCTCTGG GATTACAAAA TTTGTGAAAG
  961 ATTGACTGGT TATTCTTAAA CTATGTTGCT CCCTTTTACG CTAATGTGGC ATACGCTGCC
 1021 TTAATTGCCC TTTGTATCCA TGCCTAATGC CATGCCCGAT ATGCATTCAA TTTTTCTCGT
1081 CCTTGTAAAT AAAT
//
Primer: CMV-Forward RDB12871_A4J9e.seq
>12871_12871_A4J9_1_CMV-Forward_C02_08_ABI24.ab1
    1 CTTTTAAACT CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
  121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
  181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
  241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
  301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
  361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG
  421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC
  481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG
  541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA
  601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA
  661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG
  721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGCGGCCG
  781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGGACAAT TGGAGAAGTG AATTATATAA
  841 AATATAAAGT AGTAAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAAGG CAAAGAGAAA
  901 GAGTGGTGCA GAGAGAAAAA AAGAGCAGTG GGGAATAGGA GCTTTTGTTC CTTTGGGTTC
  961 TTGGGAGCAG CAGGAAGCAC TTATGGGGGC GCAGGCGTCA ATGAACGGCT GAACGGTTAA
1021 CAGGCAGACC ATTAATTGTC TGGTAATAGT TGCAAGTCAA GCCAGGAAAC CAAA
//
Primer: BGH_rev2 RDB12871_A4J9f.seq
>12871_12871_A4J9_1_BGH_rev2_D02_11_ABI24.ab1
    1 CTATCGGTGC AGGAGGGCAC GGGGGAGGGG CAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGAAT AACTTCGTAT
  301 AATGTATGCT ATACGAAGTT ATGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA
  361 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC
  421 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC
  481 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA
  541 GGTCTCGACG GTATCGATGC GGGGAGGCGG CCCAAAGGGA GATCCGACTC GTCTGAGGGC
  601 GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG
  661 CTGGATTGAG GGCCGAAGGG ACGTAGCAGA AGGACGTCCC GCGCAGAATC CAGGTGGCAA
  721 CACAGGCGAG CAGCCATGGA AAGGACGTCA GCTTCCCCGA CAACACCACG GAATTGTCAG
  781 TGCCCAACAG CCGAGCCCCT GTCCAGCAGC GGGCAAAGCA GGCGGCGATG AGTTCCGCCG
  841 TGGCAATAGG GAGGGGGAAA GCGAAAGTCC CGGAAAGGAG CTGACAGGTG GTGGCAATGC
  901 CCCAACCAGT GGGGGTTGCG TCAGCAAACA CAGTGCACAC CACGCCACGT TTGCCCTGAC
  961 AACGGCCACA ACTCCTCATA AGAGACAGCA CCAAGATTTA TACAAGGAGG AGAAATGAAA
 1021 GCCATACGGA GGCATAGCAT GAATACAAAG CAATAAAGCC AGCGTATCCA CATAGCGTTA
1081 AAAGGAGCAC TAGTTAGAAT ACCCAGTCAA TCGTT
//
Primer: SV40pro_F RDB12871_A4J9g.seq
>12871_12871_A4J9_1_SV40pro_F_G01_19_ABI24.ab1
    1 ACGGGTCCCT GTGACATCAG GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
  121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
  181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
  241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
  301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
  361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
  421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
  481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
  541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
  661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
  721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
  781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
  841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
  901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CCTGGGGGTG CCCTAATGAG TGAGCTAACT
  961 CACATTAAAT TTGCGTTGCG CTCACTGCCC GCTTTCCCAG TCGGGAAACC CTGTCGTGCC
 1021 AGCTGCATTT AAATGACTCG ATCAACGGCC GCGGGGGAGA AGCCGATTGC GTATTGGCGC
1081 CCTCTTCGCA TTCCTTCGGC GT
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.09

GNP_filter3_RDBDEP_html_180823.pl