Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with hKO1 marker.
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Other clones in our bank
External Database Reference sequence |
Sequence (full) ![]() |
RDB12870zzt01.seq compiled by us |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
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Ordering | Order form [Credit Card Payment ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() Please visit Ordering instruction.[link] |
Remarks | Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
提供条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
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提供依頼 | 提供依頼書 [Word ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB12870 | CSII-EF-RfA-IRES2-hKO1 | DNA solution |
Materials & Methods section:
The CSII-EF-RfA-IRES2-hKO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12870). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Lentiviral plasmid list (English text) |
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Featured content | Lentivirus Vector (English text) |
Featured content | Lentivirus Vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12870_A9Cip1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pAxEF_F1 (Pr0041) Region: RfA(attR1,CmR) Sequence file: RDB12870_A9Cih.seq ![]() |
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>12870_12870_A9Ci_1_pAxEF_F1_A12_03_ABI24.ab1 1 NNNNNNNNNN NCNTTNNNNN GTCGNNNNNN GCTACCGGTC TCGAGAATTC TGCAGCGGCC 61 ATCACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA 121 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG 181 TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATA 241 ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG 301 AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT 361 TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA 421 CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA 481 TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC 541 TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT 601 TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC 661 AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTNCC NNAAGGGTTT ATTGAGANNA 721 TGTTTTTCGT CTCAGCCNAT CCCTGGGTGA GTTTCACCAG TTTTGANTTN AACGTGGCCN 781 NTATGGACAA CTTCNNCGCC CCCGTTTTCN NCNTGGGCAA NTATTATNCG CNNNNNGACA 841 ANGNGCTGAN GCCGCTGGNG ATTCNNNNNC NTCNTGCCNN TTTGTGNNNN CNNNCATGTC 901 NNNNNNNNGC NNNANGNNNN NNNNCNNNNC TGCNNTNANN NNNNNGNNNG NNNTAANNNN 961 NNNNNN // |
Primer: ccdB-C_F (Pr0136) Region: RfA(ccdB,attR2),IRES2 Sequence file: RDB12870_A9Cii.seq ![]() |
>12870_12870_A9Ci_1_ccdB-C_F_D03_12_ABI24.ab1 1 NNNNNNNNNN NNNNNNNTCG GGGNNNAAGC TGGCGCATGN TGNCCACCGA TATGGCCAGT 61 GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC 121 AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC 181 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG 241 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC 301 AGCTTTCTTG TACAAAGTGG TGATGGCCGC GGATCCGCCC CTCTCCCTCC CCCCCCCCTA 361 ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT 421 CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA 481 CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTGAATGTCG 541 TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT 601 GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT 661 AAGATACACC TGCAAANGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG ATAGTTGTGN 721 AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAANNNNGG GGNTGAANGA NGCCCNNANN 781 GTACCCCATT GTATGGGNNN CTGATCTGGG GGNNNNNNGN NCACATGCTT TACNTGTGTT 841 NNNNNCNAGG NNNAAAANAC GTCTANGNCC CCCNNANNCN NNGNANNTGG TTTTNCNTTT 901 GAAANNNCNA NNATNNTATN GNNNNNNGGN ANNCCCCNNN NNNNNNN // |
Primer: PRE_rev (Pr0250) Region: WPRE,hKO Sequence file: RDB12870_A9Cij.seq ![]() |
>12870_12870_B2HfF_PRE_rev_E05_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNGN NNNNNAGCNN CNTAGTTAAG AATACCAGTC AATCTTTCAC 61 NAATTTTGTA ATCCANNAGG TTGATTATCG ATAAGCTTGA TCCCTCGATG TTAACTCTAG 121 CCTGCAGTCA GCAGTGGGCC ACGGCGTCCT CCACCAGCTC GGTGATGTTG CCCTCGGTCT 181 TCCTCACCAG CCTGTGGCCG ATGAAGTGGC TCTGGGGCAT CTTCAGGATC TTCTTGGCGG 241 CCTTGTAGGT GGTCTTGAAC TGGCACTTGT GGTTGCCGCC GCCGGCCAGC TTCAGGTACA 301 TGGTCACGTC GCCCTTCAGC ACGCCGTCGC AGGTGGTGAT CTTCTCGGTG CTGGGCTCCC 361 AGTCGCTGCT CTGGTTCTGC ATCACGGGGC CGTCGGCGGG GAAGTTCACG CCCACGAACT 421 TGCTCTTGTG CTCGAAGCAG TTGCCCCTCA GGCTGATGTG GGCGCTCACG GCGGCGAAGC 481 CGCCGTCCTC GAACTGGAGG CTCCTCTCCC AGCTCAGGCC CTCGGGGAAG GCCTGCTTGA 541 AGTAGTCGGG GATCTCCTCG GGGTACTTGG TGAAGGGCCT GTGGCCGTAG CAGAAGGTGT 601 GGCTCACCAG GTCGAAGCTG AAGGGCATGG GGCCGCCCTT GGCCATTGTC ACCCTCAGGG 661 TCATCTCCTG GTGGCCCTCG TAGGGCTTGC CGGTGCCCTC GCCCTCCACG GTGAACTCGT 721 GGCCGTTCAC GCTGCCGTCC ATGAAGTACT TCATCTTCAT CTCGGGCTTG ATCACGCTCA 781 CCATGGTGGG GATCCCCGTG GCCNTATTAT CATCGTGTTT TTCAAAGGAA AACCACGTCC 841 CCGTGGTTCG GGGGGCCTAG ANGTTTTTTT AACCTCGACT AAACACATGT AAAGCATGTG 901 TACCGAGGNC CCAGATCANA TCCNNTACNA NGGGGNANCT TCTGGGCATC NNNCNNCCCC 961 TNGNTNAATA CNCTTGNNNA NANCCATTTG ANTCTTTNCN NNACTNNNCN NCTCNCNNCN 1021 NGNCNCTGGG GNNGNGNNNN NNTTNCNNNN NANNNNANNN NCNNNNNNTT NNNNNNNNNN // |
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB12870_A9Cik.seq ![]() |
>12870_12870_B2HfF_CMV_Forward_F05_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN TGNNNCNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT 61 ATAANCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG 301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC 361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA 421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT 481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG 541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA 601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT 661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA 721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG 781 CTGATCTTCA GACCTGGAGG ANGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA 841 TATAAAGTAG TAAAAATTGA ACCATTANGA GTAGCACCCA CCNAGGNNAA GANAANAGTG 901 GTGNANAGAG AAAAAAGANC AGTGGGAATA GGANCTTTGT TCCTTGGGNT NNNTGGGANC 961 ANCNNNANCN CTATGGGCNC AGCGTCNATG ANNCTGANNN NANNNNNNGA NAATTATNGN 1021 CTGGNNNNAN NGNNNCNNCN NNNNNTTTGN NGNNGNCTNT NNNNNNNNNN // |
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi,5'LTR Sequence file: RDB12870_A9Cil.seq ![]() |
>12870_12870_A9Ci_1_HIV-gp_R1_H04_08_ABI08.ab1 1 NNNNNNNNNN NNCNNNGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG 61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT 121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CCTTGCTGTG CGGTGGTCTT 181 ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT 241 CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA 301 TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC 361 TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT 421 TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT 481 TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT 541 CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC 601 AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT 661 CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGANGGA TCTCTAGTTA 721 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA 781 GGCTTAAGCA GTGNGTTCCC TAGTTAGCCA GAGANCTCCC ANGCTCAGAT CTGGTCTAAC 841 NAGAGAGANC CANNACAGGC AAAANGCGCT GCTTATATAG ANCTCCCACC GNACNCGCCN 901 ANCGCCNATT TNCGNCNATG GGGCGNNNNN GNNACNANNT TTNNGNNAAG TCCCNNNGAN 961 TTNNNNGNCN NAANNNANNN NCNNNNNNNN NN // |
Primer: env_F (Pr0258) Region: cPPT_CTS,EF-1 alpha pro Sequence file: RDB12870_A9Cim.seq ![]() |
>12870_12870_B2HfF_bGH_rev3_G05_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNG GGGNNGGGNN NACAACNGAT GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA 121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA 181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT 241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG 301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT 361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT 421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT 481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT 541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC 601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG 661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC 721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC 781 GACGGTATCG ATGCGGGGAN GCGGNCCAAA GGGAGATCCG ACTCGTCTGA NGGCGANNNG 841 AAGANNCGGA AGANGNCGCA NANCCNGCAG CANGNCGCGG GANNGANNGN CCGCTGGATT 901 GANGNNCGAA GGGNNNTANC ANANNNNNTC CCGCGCANAA TCCANNTGGC AACNCNNNCN 961 ANCANCCNTG GNAAGGANGT CNNCTTCCCC GANANCNCCN NNGAANTGTC ANTNNCCANA 1021 GCCNANCCCC NGNNCANCAN CNGGNNNNNN NNNN // |
Primer: bGH_rev3 (Pr0885) Region: bGH pA,3'LTR,WPRE Sequence file: RDB12870_A9Cin.seq ![]() |
>12870_12870_A9Ci_1_env_F_G12_21_ABI24.ab1 1 NNNNNNNNNN NNNNTTCGNN NNGTNNNGGN NCTACNAATG GCAGTATTCA TCCCACAATT 61 TNAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG 121 CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG 181 TTTATTACAG GGACAGCAGA AATTCACTTT GAATTAATTC AAGCTTCGTG AGGCTCCGGT 241 GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC 301 GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG 361 TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC 421 GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT 481 TCCCGCGGGC CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC 541 TGGCTCCAGT ACGTGATTCT TGATCCCGAG CTGGAGCCAG GGGCGGGCCT TGCGCTTTAG 601 GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC 661 GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA GCCATTTAAA 721 ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA AATGCGGGCC 781 ANGATCTGCA CNCTGGTATT TCNNNTTTTG GGGNCGCNGG CGGCGANNNG GCCCGTGCGT 841 CCCANCGCAC ATGTTCNGNG ANGNGGGGCC NGCNANCGCN NNCNNNNANA ATCNNANGGG 901 GGTANTNNCN NNCTNNNCNN NNNNGNTCTG GNNNNTNNNN NNNNNN // |
Primer: SV40pro_F_V2 (Pr0154) Region: EM7 pro,Zeo,SV40 pA Sequence file: RDB12870_A9Cio.seq ![]() |
>12870_12870_A9Ci_1_SV40pro_F_V2_F12_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNTTTTGC NNNNNNTCCC GGGNGCTTGT ATATCCNTTT 61 TCGGATCTGA TCNNNCNNGN GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT 121 AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA 181 CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG 241 ACTTCGTGGA GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG 301 CGGTCCAGGA CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG 361 ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC 421 CGGCCATGAC CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG 481 CCGGCAACTG CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG 541 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 601 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 661 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 721 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CNNTGTATCT TATCATGTCT 781 GTATACCGNC NANCTCTAGC TAGAGCNNGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 841 GAAATTGTNA TCCGCTCANN NTTNCANNCA ACATACGANC CNGAANNNTN AANGTGNNAA 901 AGNCTNNGGN GNCNNNANGN ANTNNNCNNA NNNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
reference | Iwasawa, C., Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells. Int. J. Mol. Sci. 20 (4). pii: E810 (2019). PMID 30769780. |
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2023.11.16
GNP_filter3_RDBDEP_html_231027.pl