Resource data sheet
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CSII-EF-RfA-IRES2-hKO1

Catalog number RDB12870
Resource name CSII-EF-RfA-IRES2-hKO1
Vector backbone CSII-EF-RfA-IRES2-hKO1 ()
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding hKO1.
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Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


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RDB12870 CSII-EF-RfA-IRES2-hKO1 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

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Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12870_A9Cip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 RDB12870_A9Cia.seq
>12870_12870_A9Ci_1_pAxEF_F1_A12_03_ABI24.ab1
    1 GCTCTTTTCG TCATTTCAGT GTCGTGACAC GCTACCGGTC TCGAGAATTC TGCAGCGGCC
   61 ATCACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA
  121 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG
  181 TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATA
  241 ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG
  301 AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT
  361 TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA
  421 CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA
  481 TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC
  541 TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT
  601 TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC
  661 AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGTTT ATTGAGAATA
  721 TGTTTTTCGT CTCAGCCAAT CCCTGGGTGA GTTTCACCAG TTTTGATTTA AACGTGGCCA
  781 ATATGGACAA CTTCTTCGCC CCCGTTTTCA CCATGGGCAA ATATTATACG CAAGGCGACA
  841 AGGTGCTGAT GCCGCTGGCG ATTCAGGTTC ATCATGCCGG TTTGTGATGG CTTCCATGTC
  901 GGCAGAATGC TTAATGAATT ACAACAGTAC TGCGATGAGT GGCAGGGCGG GCGTAAACGC
  961 GTGGAATCCG GCTACTAAAG CCAGATTAAC AGTATGCGTA TTGCGCGCTG ATTTTTGCGT
 1021 ATAAGAATAT ATACTGATAT GGTATACCGA GTTATGTCAA GAAGTATTGC TATGAAGCCA
 1081 GCGGTATACA GTGACAGTTG GACAGCCGTA CTGGCCTATT CAGTGTGCCT CCAAGCCGCA
1141 TA
//
Primer: BGH_rev RDB12870_A9Cib.seq
>12870_12870_A9Ci_1_BGH_rev_D12_12_ABI24.ab1
    1 CCTACGGTCT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC
   61 CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG
  121 GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC
  181 AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT GTGATGAAAT GCTAGGCGGC
  241 TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC CATCCCATGC AGGCTCACAG
  301 GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC TACCTTCTCT TGCTCAACTG
  361 GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT GATCCCTGGC CCTGGTGTGT
  421 AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT CCACAGATCA AGGATATCTT
  481 GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA
  541 GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC
  601 CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC AGCATTGGTA GCTGCTGTAT
  661 TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG GTATCGATGC GGGGAGGCGG
  721 CCCAAAGGGA GATCCGACTC GTCTGAGGGC GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG
  781 CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG CTGGATTGAG GGCCGAAAGG GACGTAGCAG
  841 ATGACGTCCC GCGCAGAATC CAGGTGGCAA CACAGGCGAG CAGCCATGGA AAGGACGTCA
  901 GCTTCCCCGA CAACACCACG GAAATTGTCA GTGCCCAACA GCCGAGCCCC TGTCCAGCAG
  961 CGGGCAAGGC AGCGCGATGA GTTCCGCCGT GGCATAGGGA GGGGGGAAGC GAAGGTCCCG
 1021 GAAGGAGCTG ACAGTTGTTG CATGGCCCCA TCAGTGGGGT GCGTTCAGCC AACACAGTGC
1081 AACAGTCACG TTGCCTGACA CGGATCCGAC TCTTCATAAG GAGAACG
//
Primer: IRES-F RDB12870_A9Cic.seq
>12870_12870_A9Ci_1_IRES-F_B12_06_ABI24.ab1
    1 GAGTCATCTG TGTTTAGTCG AGGTTAAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT
   61 GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACGGG GATCCCCACC ATGGTGAGCG
  121 TGATCAAGCC CGAGATGAAG ATGAAGTACT TCATGGACGG CAGCGTGAAC GGCCACGAGT
  181 TCACCGTGGA GGGCGAGGGC ACCGGCAAGC CCTACGAGGG CCACCAGGAG ATGACCCTGA
  241 GGGTGACAAT GGCCAAGGGC GGCCCCATGC CCTTCAGCTT CGACCTGGTG AGCCACACCT
  301 TCTGCTACGG CCACAGGCCC TTCACCAAGT ACCCCGAGGA GATCCCCGAC TACTTCAAGC
  361 AGGCCTTCCC CGAGGGCCTG AGCTGGGAGA GGAGCCTCCA GTTCGAGGAC GGCGGCTTCG
  421 CCGCCGTGAG CGCCCACATC AGCCTGAGGG GCAACTGCTT CGAGCACAAG AGCAAGTTCG
  481 TGGGCGTGAA CTTCCCCGCC GACGGCCCCG TGATGCAGAA CCAGAGCAGC GACTGGGAGC
  541 CCAGCACCGA GAAGATCACC ACCTGCGACG GCGTGCTGAA GGGCGACGTG ACCATGTACC
  601 TGAAGCTGGC CGGCGGCGGC AACCACAAGT GCCAGTTCAA GACCACCTAC AAGGCCGCCA
  661 AGAAGATCCT GAAGATGCCC CAGAGCCACT TCATCGGCCA CAGGCTGGTG AGGAAGACCG
  721 AGGGCAACAT CACCGAGCTG GTGGAGGACG CCGTGGCCCA CTGCTGACTG CAGGCTAGAG
  781 TTAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG
  841 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT
  901 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TTGTATAAAT
  961 CCTGGTTTGC TGTCTCTTTA TGAGGAGTTG TGGGCCCGTT TGTCAGGCAA CGTGGCGTGG
 1021 TGGTGCACTG TGTTGCTGAC GCAACCCCCC CACTGGGTTG GGGGCCAATG CAACACTGTC
1081 AGGCTCATTC GGAACTTCGC TTCCCCCTCC CTTATGGGCC CACCGGCCGG GA
//
Primer: ccdB-C_F RDB12870_A9Cid.seq
>12870_12870_A9Ci_1_ccdB-C_F_D03_12_ABI24.ab1
    1 GTCCAATATC GGGGGGATGA AGCTGGCGCA TGATGAACCA CCGATATGGC CAGTGTGCCG
   61 GTCTCCGTTA TCGGGGAAGA AGTGGCTGAT CTCAGCCACC GCGAAAATGA CATCAAAAAC
  121 GCCATTAACC TGATGTTCTG GGGAATATAA ATGTCAGGCT CCCTTATACA CAGCCAGTCT
  181 GCAGGTCGAC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT
  241 ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT
  301 CTTGTACAAA GTGGTGATGG CCGCGGATCC GCCCCTCTCC CTCCCCCCCC CCTAACGTTA
  361 CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT CTATATGTTA TTTTCCACCA
  421 TATTGCCGTC TTTTGGCAAT GTGAGGGCCC GGAAACCTGG CCCTGTCTTC TTGACGAGCA
  481 TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG GAATGCAAGG TCTGTTGAAT GTCGTGAAGG
  541 AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC AAACAACGTC TGTAGCGACC CTTTGCAGGC
  601 AGCGGAACCC CCCACCTGGC GACAGGTGCC TCTGCGGCCA AAAGCCACGT GTATAAGATA
  661 CACCTGCAAA GGCGGCACAA CCCCAGTGCC ACGTTGTGAG TTGGATAGTT GTGGAAAGAG
  721 TCAAATGGCT CTCCTCAAGC GTATTCAACA AAGGGGCTGA AGGATGCCCA GAAGGTACCC
  781 CATTGTATGG GATCTGATCT GGGGGCCCTC GGTACACATG CTTTACATGT GTTTAGTCGA
  841 GGTTAAAAAA ACGTCTAGGC CCCCCCGAAC CACGGGACGT GGTTTTCCTT TGAAAACACG
  901 ATGATAATAT GGGCCACGGG GATCCCCACA TGGGTGAGCC GTGATCAAAG CCCGAGATGA
  961 AGATGAAGGT ACTTCATGGA CCGGCAGCGT GGAACGGTCC ACCGAGTTCA CCGTTGGAGA
 1021 GGGCGAGGTG CTACCGTGGC TAGCTCTACG GAAGGTCAAC AGCGAATGGA CCCCTTGAAG
1081 GGTTGACAAT TGGCCAAGAG ACGGAGCCAC CCATGGCACT
//
Primer: SV40pro_F_V2 RDB12870_A9Cie.seq
>12870_12870_A9Ci_1_SV40pro_F_V2_F12_18_ABI24.ab1
    1 GTTGAGTTTT TTGGGAGGCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGT ATATCCATTT
   61 TCGGATCTGA TCACGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT
  121 AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA
  181 CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG
  241 ACTTCGTGGA GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG
  301 CGGTCCAGGA CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG
  361 ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC
  421 CGGCCATGAC CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG
  481 CCGGCAACTG CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG
  541 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT
  601 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA
  661 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT
  721 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT
  781 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT
  841 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAAGTGTAAA
  901 AGCCTGGGGT GCCCTAATGA AGTGAGCTAA CTCACATTAA TTGCGTTTGC GCTCACTTGC
  961 CGCTTTCCCA GTTCGGGAAA CCCTGTCGTT GCCAGCTGCA TTAATGAATT CGGGCCCAAA
 1021 CGCGCGGGGA AGAGCGATTG CCGTAATTGG CGCTCTTCAG CTTCCTCGCT CACTGAACTC
1081 GCTGGCCTCG ATTCGATTCC GGCCTTGACC
//
Primer: env_F RDB12870_A9Cif.seq
>12870_12870_A9Ci_1_env_F_G12_21_ABI24.ab1
    1 GCGGGGGCGG ATCATTCGAT TAGTGACGGA TCTACAAATG GCAGTATTCA TCCCACAATT
   61 TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG
  121 CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG
  181 TTTATTACAG GGACAGCAGA AATTCACTTT GAATTAATTC AAGCTTCGTG AGGCTCCGGT
  241 GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC
  301 GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG
  361 TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC
  421 GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT
  481 TCCCGCGGGC CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC
  541 TGGCTCCAGT ACGTGATTCT TGATCCCGAG CTGGAGCCAG GGGCGGGCCT TGCGCTTTAG
  601 GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC
  661 GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA GCCATTTAAA
  721 ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA AATGCGGGCC
  781 AGGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG CGGCGACGGG GCCCGTGCGT
  841 CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG GCCACCGAGA ATCGGACGGG
  901 GGTAGTCTCA AGCTGGCCCG GCCTGCTCTG GTGCCTGGCT CGCGCCGCCG TGTATCGCCC
  961 CGCCCTGGGC GGCAGGCTGG CCGGTCGGCA CCAGTTTGCG TGAGCGGAAA GATGCCGCTT
 1021 CCGGCCTTGC TCATGGCTCA AAATGAGGAC GCGGCGCCTC GGAGAGCGGC GGGTGATTCA
1081 CCTCACAGGA AGGGACCTTC CGTCCTCAGC CGATCGC
//
Primer: HIVgp_R1 RDB12870_A9Cig.seq
>12870_12870_A9Ci_1_HIV-gp_R1_H04_08_ABI08.ab1
    1 GCAGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CCTTGCTGTG CGGTGGTCTT
  181 ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT
  241 CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA
  301 TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC
  361 TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT
  421 TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT
  481 TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT
  541 CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC
  601 AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT
  661 CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA
  721 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA
  781 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC
  841 CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG ACCTCCCACC GTACACGCCT
  901 ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT TTTGGGAAAG TCCCGTTGAT
  961 TTTGGTGCCC AAAACAAACT CCCATTGACG TCAATGGGGG TGGAGACTTG GGAATCCCCG
 1021 TGAGTCAAAC CGCTATCCAC GCCATGATGT ACTGCTAAAC GCATCACCAA TGGTATAGCG
 1081 AATGAACTTA TACGTGATGT ACTGCTAGTA GGAAGGTTCC ATAAGTTCAT GTACTGGGGC
1141 CACA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2019.04.09

GNP_filter3_RDBDEP_html_190327.pl