Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-RfA-IRES2-hKO1

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with hKO1 marker.

Sequence (full)	RDB12870zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 付加的提供条件： 利用者は、hKO1蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12870_A9Cip1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 (Pr0041) Region: RfA(attR1,CmR) Sequence file: RDB12870_A9Cih.seq
>12870_12870_A9Ci_1_pAxEF_F1_A12_03_ABI24.ab1     1 NNNNNNNNNN NCNTTNNNNN GTCGNNNNNN GCTACCGGTC TCGAGAATTC TGCAGCGGCC    61 ATCACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA   121 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG   181 TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATA   241 ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG   301 AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT   361 TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA   421 CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA   481 TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC   541 TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT   601 TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC   661 AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTNCC NNAAGGGTTT ATTGAGANNA   721 TGTTTTTCGT CTCAGCCNAT CCCTGGGTGA GTTTCACCAG TTTTGANTTN AACGTGGCCN   781 NTATGGACAA CTTCNNCGCC CCCGTTTTCN NCNTGGGCAA NTATTATNCG CNNNNNGACA   841 ANGNGCTGAN GCCGCTGGNG ATTCNNNNNC NTCNTGCCNN TTTGTGNNNN CNNNCATGTC   901 NNNNNNNNGC NNNANGNNNN NNNNCNNNNC TGCNNTNANN NNNNNGNNNG NNNTAANNNN 961 NNNNNN //
Primer: ccdB-C_F (Pr0136) Region: RfA(ccdB,attR2),IRES2 Sequence file: RDB12870_A9Cii.seq
>12870_12870_A9Ci_1_ccdB-C_F_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTCG GGGNNNAAGC TGGCGCATGN TGNCCACCGA TATGGCCAGT    61 GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC   121 AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC   181 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG   241 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC   301 AGCTTTCTTG TACAAAGTGG TGATGGCCGC GGATCCGCCC CTCTCCCTCC CCCCCCCCTA   361 ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT   421 CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA   481 CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTGAATGTCG   541 TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT   601 GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT   661 AAGATACACC TGCAAANGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG ATAGTTGTGN   721 AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAANNNNGG GGNTGAANGA NGCCCNNANN   781 GTACCCCATT GTATGGGNNN CTGATCTGGG GGNNNNNNGN NCACATGCTT TACNTGTGTT   841 NNNNNCNAGG NNNAAAANAC GTCTANGNCC CCCNNANNCN NNGNANNTGG TTTTNCNTTT 901 GAAANNNCNA NNATNNTATN GNNNNNNGGN ANNCCCCNNN NNNNNNN //
Primer: PRE_rev (Pr0250) Region: WPRE,hKO Sequence file: RDB12870_A9Cij.seq
>12870_12870_B2HfF_PRE_rev_E05_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNGN NNNNNAGCNN CNTAGTTAAG AATACCAGTC AATCTTTCAC    61 NAATTTTGTA ATCCANNAGG TTGATTATCG ATAAGCTTGA TCCCTCGATG TTAACTCTAG   121 CCTGCAGTCA GCAGTGGGCC ACGGCGTCCT CCACCAGCTC GGTGATGTTG CCCTCGGTCT   181 TCCTCACCAG CCTGTGGCCG ATGAAGTGGC TCTGGGGCAT CTTCAGGATC TTCTTGGCGG   241 CCTTGTAGGT GGTCTTGAAC TGGCACTTGT GGTTGCCGCC GCCGGCCAGC TTCAGGTACA   301 TGGTCACGTC GCCCTTCAGC ACGCCGTCGC AGGTGGTGAT CTTCTCGGTG CTGGGCTCCC   361 AGTCGCTGCT CTGGTTCTGC ATCACGGGGC CGTCGGCGGG GAAGTTCACG CCCACGAACT   421 TGCTCTTGTG CTCGAAGCAG TTGCCCCTCA GGCTGATGTG GGCGCTCACG GCGGCGAAGC   481 CGCCGTCCTC GAACTGGAGG CTCCTCTCCC AGCTCAGGCC CTCGGGGAAG GCCTGCTTGA   541 AGTAGTCGGG GATCTCCTCG GGGTACTTGG TGAAGGGCCT GTGGCCGTAG CAGAAGGTGT   601 GGCTCACCAG GTCGAAGCTG AAGGGCATGG GGCCGCCCTT GGCCATTGTC ACCCTCAGGG   661 TCATCTCCTG GTGGCCCTCG TAGGGCTTGC CGGTGCCCTC GCCCTCCACG GTGAACTCGT   721 GGCCGTTCAC GCTGCCGTCC ATGAAGTACT TCATCTTCAT CTCGGGCTTG ATCACGCTCA   781 CCATGGTGGG GATCCCCGTG GCCNTATTAT CATCGTGTTT TTCAAAGGAA AACCACGTCC   841 CCGTGGTTCG GGGGGCCTAG ANGTTTTTTT AACCTCGACT AAACACATGT AAAGCATGTG   901 TACCGAGGNC CCAGATCANA TCCNNTACNA NGGGGNANCT TCTGGGCATC NNNCNNCCCC   961 TNGNTNAATA CNCTTGNNNA NANCCATTTG ANTCTTTNCN NNACTNNNCN NCTCNCNNCN  1021 NGNCNCTGGG GNNGNGNNNN NNTTNCNNNN NANNNNANNN NCNNNNNNTT NNNNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB12870_A9Cik.seq
>12870_12870_B2HfF_CMV_Forward_F05_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN TGNNNCNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAANCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA   121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT   181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT   241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG   301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC   361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA   421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT   481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG   541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA   601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT   661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA   721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG   781 CTGATCTTCA GACCTGGAGG ANGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA   841 TATAAAGTAG TAAAAATTGA ACCATTANGA GTAGCACCCA CCNAGGNNAA GANAANAGTG   901 GTGNANAGAG AAAAAAGANC AGTGGGAATA GGANCTTTGT TCCTTGGGNT NNNTGGGANC   961 ANCNNNANCN CTATGGGCNC AGCGTCNATG ANNCTGANNN NANNNNNNGA NAATTATNGN 1021 CTGGNNNNAN NGNNNCNNCN NNNNNTTTGN NGNNGNCTNT NNNNNNNNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi,5'LTR Sequence file: RDB12870_A9Cil.seq
>12870_12870_A9Ci_1_HIV-gp_R1_H04_08_ABI08.ab1     1 NNNNNNNNNN NNCNNNGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG    61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT   121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CCTTGCTGTG CGGTGGTCTT   181 ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT   241 CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA   301 TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC   361 TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT   421 TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT   481 TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT   541 CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC   601 AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT   661 CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGANGGA TCTCTAGTTA   721 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA   781 GGCTTAAGCA GTGNGTTCCC TAGTTAGCCA GAGANCTCCC ANGCTCAGAT CTGGTCTAAC   841 NAGAGAGANC CANNACAGGC AAAANGCGCT GCTTATATAG ANCTCCCACC GNACNCGCCN   901 ANCGCCNATT TNCGNCNATG GGGCGNNNNN GNNACNANNT TTNNGNNAAG TCCCNNNGAN 961 TTNNNNGNCN NAANNNANNN NCNNNNNNNN NN //
Primer: env_F (Pr0258) Region: cPPT_CTS,EF-1 alpha pro Sequence file: RDB12870_A9Cim.seq
>12870_12870_B2HfF_bGH_rev3_G05_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG GGGNNGGGNN NACAACNGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA   121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA   181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT   241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG   301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT   361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT   421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT   481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT   541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC   601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG   661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC   721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC   781 GACGGTATCG ATGCGGGGAN GCGGNCCAAA GGGAGATCCG ACTCGTCTGA NGGCGANNNG   841 AAGANNCGGA AGANGNCGCA NANCCNGCAG CANGNCGCGG GANNGANNGN CCGCTGGATT   901 GANGNNCGAA GGGNNNTANC ANANNNNNTC CCGCGCANAA TCCANNTGGC AACNCNNNCN   961 ANCANCCNTG GNAAGGANGT CNNCTTCCCC GANANCNCCN NNGAANTGTC ANTNNCCANA 1021 GCCNANCCCC NGNNCANCAN CNGGNNNNNN NNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA,3'LTR,WPRE Sequence file: RDB12870_A9Cin.seq
>12870_12870_A9Ci_1_env_F_G12_21_ABI24.ab1     1 NNNNNNNNNN NNNNTTCGNN NNGTNNNGGN NCTACNAATG GCAGTATTCA TCCCACAATT    61 TNAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG   121 CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG   181 TTTATTACAG GGACAGCAGA AATTCACTTT GAATTAATTC AAGCTTCGTG AGGCTCCGGT   241 GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC   301 GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG   361 TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC   421 GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT   481 TCCCGCGGGC CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC   541 TGGCTCCAGT ACGTGATTCT TGATCCCGAG CTGGAGCCAG GGGCGGGCCT TGCGCTTTAG   601 GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC   661 GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA GCCATTTAAA   721 ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA AATGCGGGCC   781 ANGATCTGCA CNCTGGTATT TCNNNTTTTG GGGNCGCNGG CGGCGANNNG GCCCGTGCGT   841 CCCANCGCAC ATGTTCNGNG ANGNGGGGCC NGCNANCGCN NNCNNNNANA ATCNNANGGG 901 GGTANTNNCN NNCTNNNCNN NNNNGNTCTG GNNNNTNNNN NNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: EM7 pro,Zeo,SV40 pA Sequence file: RDB12870_A9Cio.seq
>12870_12870_A9Ci_1_SV40pro_F_V2_F12_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNTTTTGC NNNNNNTCCC GGGNGCTTGT ATATCCNTTT    61 TCGGATCTGA TCNNNCNNGN GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT   121 AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA   181 CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG   241 ACTTCGTGGA GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG   301 CGGTCCAGGA CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG   361 ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC   421 CGGCCATGAC CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG   481 CCGGCAACTG CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG   541 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT   601 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA   661 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT   721 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CNNTGTATCT TATCATGTCT   781 GTATACCGNC NANCTCTAGC TAGAGCNNGG CGTAATCATG GTCATAGCTG TTTCCTGTGT   841 GAAATTGTNA TCCGCTCANN NTTNCANNCA ACATACGANC CNGAANNNTN AANGTGNNAA 901 AGNCTNNGGN GNCNNNANGN ANTNNNCNNA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl