Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-RfA-IRES-Puro

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with puromycin resistance marker.

Sequence (full)	RDB12869zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12869_A9I3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1a pro, attR1, lac UV5 pro, CmR 5' Sequence file: RDB12869_A9I3a.seq
>12869_12869_A9I3_1_EF1a-C_F_A07_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGAGTTTGG NNCTTGGTTC NTTCTCNAGC CTCAGACAGT    61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAACA CGCTACCGGT CTCGAGAATT   121 ATCACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA   181 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG   241 TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATA   301 ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG   361 AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT   421 TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA   481 CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA   541 TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC   601 TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT   661 TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC   721 AAGATGTGNC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGNTT ATTGAGAATA   781 TGTTTTTCGT CTCAGCCNAT CNCTGGGTGA GTTTCACCAG TTTTGATTTA ANCGTGGCCA   841 ATATGGACAA CTTCTTCGCC CCCGTTTTCA CCANGGGCAA ATATTATACG CAAGGCGNNA   901 ANGNGCTGAT GNCGCNGGCG ANTCNNGTNC ATCATGCCGT TNGTGATGGC TNCNNTGNNG   961 NNANANNGCN TNNNNGANTT ACAACAGNAC TGNNANNANN GNNANGNNNG GGNNNNNANN 1021 NNNNNNNN //
Primer: Cm_C_F (Pr0476) Region: ccdB, attR2 Sequence file: RDB12869_A9I3b.seq
>12869_12869_A9I3_1_Cm_C_F_B07_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNTTNNNNN NNNCNNGANA ACNGTATGCG TATTTGCGCG    61 CTGATTTTTG CGGTATAAGA ATATATACTG ATATGTATAC CCGAAGTATG TCAAAAAGAG   121 GTATGCTATG AAGCAGCGTA TTACAGTGAC AGTTGACAGC GACAGCTATC AGTTGCTCAA   181 GGCATATATG ATGTCAATAT CTCCGGTCTG GTAAGCACAA CCATGCAGAA TGAAGCCCGT   241 CGTCTGCGTG CCGAACGCTG GAAAGCGGAA AATCAGGAAG GGATGGCTGA GGTCGCCCGG   301 TTTATTGAAA TGAACGGCTC TTTTGCTGAC GAGAACAGGG GCTGGTGAAA TGCAGTTTAA   361 GGTTTACACC TATAAAAGAG AGAGCCGTTA TCGTCTGTTT GTGGATGTAC AGAGTGATAT   421 TATTGACACG CCCGGGCGAC GGATGGTGAT CCCCCTGGCC AGTGCACGTC TGCTGTCAGA   481 TAAAGTCTCC CGTGAACTTT ACCCGGTGGT GCATATCGGG GATGAAAGCT GGCGCATGAT   541 GACCACCGAT ATGGCCAGTG TGCCGGTCTC CGTTATCGGG GAAGAAGTGG CTGATCTCAG   601 CCACCGCGAA AATGACATCA AAAACGCCAT TAACCTGATG TTCTGGGGAA TATAAATGTC   661 AGGCTCCCTT ATACACAGCC AGTCTGCAGG TCGACCATAG TGACTGGATA TGTTGTGTTT   721 TACAGTATTA TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT   781 CATTTTACGT TTCTCGTTCA GCTTTCTTGT ACNAAGTGGT GATAATTCTG CAGCGGCCGC   841 GGATCCTCTA NACTGGANTT AATTCGCTGT CTGCGANGGN NAGCTGTTGG GGTGAGTACT   901 CCNNCTCAAA ANCGGGCATG ANTTCTGCGC TNNNANTGNC AGTTTCCNAA ANCGANGANG   961 ANTTGATATN CACNNNGCCN NNNGNGATGN NNTTGNNGNT GGNNNCNNCC ATCTGGNCAN 1021 AAAANNNNNN TTTTNNNNNN NNNN //
Primer: IRES-F (Pr0036) Region: IRES, PuroR Sequence file: RDB12869_A9I3c.seq
>12869_12869_A9I3_1_IRES-F_C07_07_ABI24.ab1     1 NNNNNNNNNN NNNNNTNNGT CGANGNNNNN NAACGTCTAG GCCCCCCGAA CCNCGGGGNC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA GCTTGCCACA ACCCACAAGG AGACGACCTT   121 CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG   181 TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGACCCGG   241 ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG   301 ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG   361 AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG   421 GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG   481 AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG   541 GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC   601 TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG   661 CCGACGTCGA NGTGCCCGAA NGANCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT   721 GAAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG   781 ANTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGANACG CTGCTTTAAT   841 GNCTTTGTAT CATGCTATTG CTTCCCGNAT GGNTTTCATT TTCTCCTCCT NGTATAAATC   901 CTGGTTGCTG TCTCTTTATG ANGANTTGTG GCCNGTTGTC NNNNAACGNG GCGNNNNNNG   961 CACNGNNTTN NNTGNNGCNN NCCCCNNCNN NNTNNGGGGC ATTGNNNCCN NCNGNCANNN 1021 NNNTTNNNNG GNNNNTNNNN TTNNNNNNNN NCNNNNNNNN NN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5'LTR, HIV-1psi Sequence file: RDB12869_A9I3d.seq
>12869_12869_A9I3_1_CMV_Forward_D07_10_ABI24.ab1     1 NNNNNNNNNN NNNNCNNNNN NNCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT   121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA   181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA   241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA   301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG   361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT   421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG   481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG   541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA   601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT   661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT   721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG   781 ATCTTCAGAC CTGGANGANG AGANATGANG GANAATTGGA GAAGTGAATT ATATAAATAT   841 AAAGTAGTAA AAATTGAACC NTTANGAGTA GCACCCACCA NNGCAAAGAG AAGANTGGNG   901 CAGANAGAAA AAAAGAGCAN NGGGAATAGG NNCTTTGTTC CNNNGNNTCT TGGNNNCAGC 961 NNNAAGCNCT NNNGGCGCNN CNNNNNTGNN NNNNNNNGNN NNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH p(A), 3'LTR, PRE Sequence file: RDB12869_A9I3e.seq
>12869_12869_A9I3_1_BGH_rev2_E07_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA   121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA   181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT   241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG   301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT   361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT   421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT   481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT   541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC   601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG   661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC   721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC   781 GACGGTATCG ATGCGGGGAN GCGGNCCAAA GGGAGATCCG ANTCGTCTGA NGGCGAANGC   841 GAAGANGCGG AANANGNNGC ANANCCNGCA GCANGNCGCG GGAANGAANG TCCGCTGGNN   901 TGANGGNCGA ANGGNNGTAN CANANNGNNN TCCCGCGCAN AATCCANGNG GNNACNCNGG 961 NNANCANNNN TGGNAAGGNN GTCNNCTTNC NNNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori, EM7 pro, Zeo, SV40 p(A) Sequence file: RDB12869_A9I3f.seq
>12869_12869_A9I3_1_SV40pro_F_F07_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNANNAGTGA GGNNGNTTTT TTGGAGGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT   121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG   181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT   241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG   301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA   361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG   421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT   481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG   541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG   601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC   661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA   721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT   781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GNNCTCTAGC TAGAGCTTGN   841 CGTAATCATG GNCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTNCACACA   901 ACATACGANC NNGAAGCATA AAGTGTAAAG CCNGGGGNGN CNNNTGAGTG ANCNAACNCN   961 CATTAATTGN GTTGCNCNCA CNGNCNNNNT TNNAGNCNGN NAANNNNNNN NNGNCNNNNN 1021 NNNNN //
Primer: cPPT_CTS-E_R (Pr0725) Region: cPPT/CTS, RRE Sequence file: RDB12869_A9I3g.seq
>12869_12869_A9I3_1_CPPT_CTS-E_R_G06_21_ABI24.ab1     1 NNNNNNNNNN TNGNTTTTGN NNTTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA    61 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA   121 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT   181 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC   241 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT   301 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT   361 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA   421 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG   481 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA   541 TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGAGCAACCC   601 CAAATCCCCA GGAGCTGTTG ATCCTTTAGG TATCTTTCCA CAGCCAGGAT TCTTGCCTGG   661 AGCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT GTTGCGCCTC AATAGCCCTC   721 AGCAAATTGT TCTGCTGCTG CACTATACCA GACAATAATT GTCTGGCCTG TACCGTCAGC   781 GTCATTGACG CTGCGCCCAT AGTGCTTCCT GCTGCTCCCN ANAACCCAAG GAACAAAGCT   841 CCNATTCCCA CTNCNCTTTT TTTCTCTCTG CACCACTCTT CTCTTNGCCT NGGNGGGNGC   901 TACNNCNAAN NGTTCAATTT TTACNACTTT ATATTTATAT NATTNNCTTC NCCNATTNNC 961 CCNNATATCT NNNNNNNNNN //
Primer: ccdb-M_F (Pr0951) Region: ccdB, attR2,chimeric intron,IRES Sequence file: RDB12869_A9I3h.seq
>12869_12869_B1LkF_ccdb-M_F_C05_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNNNANNG GCTGNNCTNN GCNNCCGCGA AAATGACATC    61 NAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC   121 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG   181 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC   241 AGCTTTCTTG TACAAAGTGG TGATAATTCT GCAGCGGCCG CGGATCCTCT AGACTGGAAT   301 TAATTCGCTG TCTGCGAGGG CCAGCTGTTG GGGTGAGTAC TCCCTCTCAA AAGCGGGCAT   361 GACTTCTGCG CTAAGATTGT CAGTTTCCAA AAACGAGGAG GATTTGATAT TCACCTGGCC   421 CGCGGTGATG CCTTTGAGGG TGGCCGCGTC CATCTGGTCA GAAAAGACAA TCTTTTTGTT   481 GTCAAGCTTG AGGTGTGGCA GGCTTGGGAT CTGGCCATAC ACTTGAGTGA CAATGACATC   541 CACTTTGCCT TTCTCTCCAC AGGTGTCCAC TCCCAGGTCC AACTGCAGGT CGAGCATGCA   601 TCTAGGGCGG CCAATTCCGC CCCTCTCCCC NNNNNNCCTC TCCCTCCCCC CCCCCTAACG   661 TTACTGGCCG AAGCCGCTTG GAATAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA   721 CCNTATTGCC GTCTTTTGGC AATGTGAGGG CCCGGAAANC TGGCCCTGTC TTCTTGACNA   781 NNNNTNNTAN GNNNNNNTTC NNCTCTCGCN NAAGGNATGC NNNNGNNNNN NGAANGTCNT   841 GAANGAAGCA NNTCNTCTNG NANCTTCNNN NNNANAAACA NCGTNNGTNN NGANCNNTTN   901 NNNNGCNNNN NAACCCCCCC ANCNGGNCGN NNNGNNNCNT CNGCNNNNNN AAGNNACNNN   961 GTANNAANAN NNNNCCTGNN AANNNNGGNA CNANCCCCNN TGNCNNNGTN GTNANNTNGG  1021 ANNANTTNNN GNNAANANNC NANNNGNNNN NCNNNNNGNN NNNNTNNNNC NNNGGGNNNN 1081 ANNNNNNNNC NNNNNNNNCC CNNNTNNNNN NNGNNNNNNN NNN //
Primer: PuroR-rev (Pr0423) Region: IRES Sequence file: RDB12869_A9I3i.seq
>12869_12869_B1LkF_PuroR-rev_E05_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNNN NNNNNNNGCT TATCNTCGTG TTTTTCNAAG    61 GAAAACCACG TCCCCGTGGT TCGGGGGGCC TAGACGTTTT TTTAACCTCG ACTAAACACA   121 TGTAAAGCAT GTGCACCGAG GCCCCAGATC AGATCCCATA CAATGGGGTA CCTTCTGGGC   181 ATCCTTCAGC CCCTTGTTGA ATACGCTTGA GGAGAGCCAT TTGACTCTTT CCACAACTAT   241 CCAACTCACA ACGTGGCACT GGGGTTGTGC CGCCTTTGCA GGTGTATCTT ATACACGTGG   301 CTTTTGGCCG CAGAGGCACC TGTCGCCAGG TGGGGGGTTC CGCTGCCTGC AAAGGGTCGC   361 TACAGACGTT GTTTGTCTTC AAGAAGCTTC CAGAGGAACT GCTTCCTTCA CGACATTCAA   421 CAGACCTTGC ATTCCTTTGG CGAGAGGGGA AAGACCCCTA GGAATGCTCG TCAAGAAGAC   481 AGGGCCAGGT TTCCGGGCCC TCACATTGCC AAAAGACGGC AATATGGTGG AAAATAACAT   541 ATAGACAAAC GCACACCGGC CTTATTCCAA GCGGCTTCGG CCAGTAACGT TAGGGGGGGG 601 GGAGGGAGAG GGGGGGGN //
Primer: mCherry-check_R5 (Pr1024) Region: PuroR Sequence file: RDB12869_A9I3j.seq
>12869_12869_B1LkF_mCherry-check_R5_F05_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGTAAAANG ANNNNACATA    61 NNTNANAATA CCAGTCNATC TTTCNCAAAT TTTGTAATCC AGAGGTTGAT TATCGAATAA   121 GCTTGATCCC TCGATGTTTC AGGCACCGGG CTTGCGGGTC ATGCACCAGG TGCGCGGTCC   181 TTCGGGCACC TCGACGTCGG CGGTGACGGT GAAGCCGAGC CGCTCGTAGA AGGGGAGGTT   241 GCGGGGCGCG GAGGTCTCCA GGAAGGCGGG CACCCCGGCG CGCTCGGCCG CCTCCACTCC   301 GGGGAGCACG ACGGCGCTGC CCAGACCCTT GCCCTGGTGG TCGGGCGAGA CGCCGACGGT   361 GGCCAGGAAC CACGCGGGCT CCTTGGGCCG GTGCGGCGCC AGGAGGCCTT CCATCTGTTG   421 CTGCGCGGCC AGCCGGGAAC CGCTCAACTC GGCCATGCGC GGGCCGATCT CGGCGAACAC   481 CGCCCCCGCT TCGACGCTCT CCGGCGTGGT CCAGACCGCC ACCGCGGCGC CGTCGTCCGC   541 GACCCACACC TTGCCGATGT CGAGCCCGAC GCGCGTGAGG AAGAGTTCTT GCAGCTCGGT   601 GACCCGCTCG ATGTGGCGGT CCGGGTCGAC GGTGTGGCGC GTGGCGGGGT AGTCGGCGAA   661 CGCGGCGGCG AGGGTGCGTA CGGCCCGGGG GNANGTCGTC GNNNNNGGCG AGGCGCACCG   721 TGGGCTTGTA CTCGGTCATG GAANNGNNNN CTCCTTGTGG GTTGTGNNAA GCTTATCATC   781 GNGNTTTTCA AAGGNAAAAC CNNNGTCCCC GTGGNTCGGG GGGGCCTANA CGTTTTTTTA   841 ACCTCNNCTA ANCACNNTGN NAAAGCANGT GCACCGANGC CCNNNATCAG ATNCCATACA   901 ATGGGGNTAC CTTCTGGGNC NTCCTTCANC CCCNTNGTNN AANNNNNNTN NAGNANANNC   961 NNNTTTGACT CTTNNNNNNN ACTATNCNNC NNNNNANNGT GGNNCTGGGG TTGTGNCGCC  1021 TTTNNNNNNN NANCNTANNC NCGNNNNNTT TTGNNNGNNN ANGNNNNNNG NNNGNNNNGT 1081 NNNGGGGNTN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl