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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-RfA-IRES-Puro

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with puromycin resistance marker.

Catalog number RDB12869
Resource name CSII-EF-RfA-IRES-Puro
Clone info. Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with puromycin resistance marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-RfA-IRES-Puro (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction.[link] 
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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12869 CSII-EF-RfA-IRES-Puro DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12869_A9I3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Region: EF-1a pro, attR1, lac UV5 pro, CmR 5'
Sequence file: RDB12869_A9I3a.seq check
>12869_12869_A9I3_1_EF1a-C_F_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGAGTTTGG NNCTTGGTTC NTTCTCNAGC CTCAGACAGT
   61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAACA CGCTACCGGT CTCGAGAATT
  121 ATCACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA
  181 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG
  241 TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATA
  301 ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG
  361 AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT
  421 TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA
  481 CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA
  541 TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC
  601 TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT
  661 TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC
  721 AAGATGTGNC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGNTT ATTGAGAATA
  781 TGTTTTTCGT CTCAGCCNAT CNCTGGGTGA GTTTCACCAG TTTTGATTTA ANCGTGGCCA
  841 ATATGGACAA CTTCTTCGCC CCCGTTTTCA CCANGGGCAA ATATTATACG CAAGGCGNNA
  901 ANGNGCTGAT GNCGCNGGCG ANTCNNGTNC ATCATGCCGT TNGTGATGGC TNCNNTGNNG
  961 NNANANNGCN TNNNNGANTT ACAACAGNAC TGNNANNANN GNNANGNNNG GGNNNNNANN
1021 NNNNNNNN
//
Primer: Cm_C_F
Region: ccdB, attR2
Sequence file: RDB12869_A9I3b.seq check
>12869_12869_A9I3_1_Cm_C_F_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNTTNNNNN NNNCNNGANA ACNGTATGCG TATTTGCGCG
   61 CTGATTTTTG CGGTATAAGA ATATATACTG ATATGTATAC CCGAAGTATG TCAAAAAGAG
  121 GTATGCTATG AAGCAGCGTA TTACAGTGAC AGTTGACAGC GACAGCTATC AGTTGCTCAA
  181 GGCATATATG ATGTCAATAT CTCCGGTCTG GTAAGCACAA CCATGCAGAA TGAAGCCCGT
  241 CGTCTGCGTG CCGAACGCTG GAAAGCGGAA AATCAGGAAG GGATGGCTGA GGTCGCCCGG
  301 TTTATTGAAA TGAACGGCTC TTTTGCTGAC GAGAACAGGG GCTGGTGAAA TGCAGTTTAA
  361 GGTTTACACC TATAAAAGAG AGAGCCGTTA TCGTCTGTTT GTGGATGTAC AGAGTGATAT
  421 TATTGACACG CCCGGGCGAC GGATGGTGAT CCCCCTGGCC AGTGCACGTC TGCTGTCAGA
  481 TAAAGTCTCC CGTGAACTTT ACCCGGTGGT GCATATCGGG GATGAAAGCT GGCGCATGAT
  541 GACCACCGAT ATGGCCAGTG TGCCGGTCTC CGTTATCGGG GAAGAAGTGG CTGATCTCAG
  601 CCACCGCGAA AATGACATCA AAAACGCCAT TAACCTGATG TTCTGGGGAA TATAAATGTC
  661 AGGCTCCCTT ATACACAGCC AGTCTGCAGG TCGACCATAG TGACTGGATA TGTTGTGTTT
  721 TACAGTATTA TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT
  781 CATTTTACGT TTCTCGTTCA GCTTTCTTGT ACNAAGTGGT GATAATTCTG CAGCGGCCGC
  841 GGATCCTCTA NACTGGANTT AATTCGCTGT CTGCGANGGN NAGCTGTTGG GGTGAGTACT
  901 CCNNCTCAAA ANCGGGCATG ANTTCTGCGC TNNNANTGNC AGTTTCCNAA ANCGANGANG
  961 ANTTGATATN CACNNNGCCN NNNGNGATGN NNTTGNNGNT GGNNNCNNCC ATCTGGNCAN
1021 AAAANNNNNN TTTTNNNNNN NNNN
//
Primer: IRES-F
Region: IRES, PuroR
Sequence file: RDB12869_A9I3c.seq check
>12869_12869_A9I3_1_IRES-F_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNTNNGT CGANGNNNNN NAACGTCTAG GCCCCCCGAA CCNCGGGGNC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA GCTTGCCACA ACCCACAAGG AGACGACCTT
  121 CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG
  181 TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGACCCGG
  241 ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG
  301 ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG
  361 AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG
  421 GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG
  481 AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG
  541 GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC
  601 TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG
  661 CCGACGTCGA NGTGCCCGAA NGANCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT
  721 GAAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG
  781 ANTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGANACG CTGCTTTAAT
  841 GNCTTTGTAT CATGCTATTG CTTCCCGNAT GGNTTTCATT TTCTCCTCCT NGTATAAATC
  901 CTGGTTGCTG TCTCTTTATG ANGANTTGTG GCCNGTTGTC NNNNAACGNG GCGNNNNNNG
  961 CACNGNNTTN NNTGNNGCNN NCCCCNNCNN NNTNNGGGGC ATTGNNNCCN NCNGNCANNN
1021 NNNTTNNNNG GNNNNTNNNN TTNNNNNNNN NCNNNNNNNN NN
//
Primer: CMV_Forward
Region: CMV pro, 5'LTR, HIV-1psi
Sequence file: RDB12869_A9I3d.seq check
>12869_12869_A9I3_1_CMV_Forward_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNNNN NNCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
  721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG
  781 ATCTTCAGAC CTGGANGANG AGANATGANG GANAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAACC NTTANGAGTA GCACCCACCA NNGCAAAGAG AAGANTGGNG
  901 CAGANAGAAA AAAAGAGCAN NGGGAATAGG NNCTTTGTTC CNNNGNNTCT TGGNNNCAGC
961 NNNAAGCNCT NNNGGCGCNN CNNNNNTGNN NNNNNNNGNN NNNNNNNN
//
Primer: BGH_rev2
Region: bGH p(A), 3'LTR, PRE
Sequence file: RDB12869_A9I3e.seq check
>12869_12869_A9I3_1_BGH_rev2_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
  121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
  181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
  241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
  301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
  361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
  421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
  481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
  541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
  601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
  661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
  721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
  781 GACGGTATCG ATGCGGGGAN GCGGNCCAAA GGGAGATCCG ANTCGTCTGA NGGCGAANGC
  841 GAAGANGCGG AANANGNNGC ANANCCNGCA GCANGNCGCG GGAANGAANG TCCGCTGGNN
  901 TGANGGNCGA ANGGNNGTAN CANANNGNNN TCCCGCGCAN AATCCANGNG GNNACNCNGG
961 NNANCANNNN TGGNAAGGNN GTCNNCTTNC NNNNNNNNNN
//
Primer: SV40pro_F
Region: SV40 pro_ori, EM7 pro, Zeo, SV40 p(A)
Sequence file: RDB12869_A9I3f.seq check
>12869_12869_A9I3_1_SV40pro_F_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNANNAGTGA GGNNGNTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GNNCTCTAGC TAGAGCTTGN
  841 CGTAATCATG GNCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTNCACACA
  901 ACATACGANC NNGAAGCATA AAGTGTAAAG CCNGGGGNGN CNNNTGAGTG ANCNAACNCN
  961 CATTAATTGN GTTGCNCNCA CNGNCNNNNT TNNAGNCNGN NAANNNNNNN NNGNCNNNNN
1021 NNNNN
//
Primer: cPPT_CTS-E_R
Region: cPPT/CTS, RRE
Sequence file: RDB12869_A9I3g.seq check
>12869_12869_A9I3_1_CPPT_CTS-E_R_G06_21_ABI24.ab1
    1 NNNNNNNNNN TNGNTTTTGN NNTTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA
   61 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA
  121 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT
  181 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC
  241 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT
  301 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT
  361 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA
  421 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG
  481 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA
  541 TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGAGCAACCC
  601 CAAATCCCCA GGAGCTGTTG ATCCTTTAGG TATCTTTCCA CAGCCAGGAT TCTTGCCTGG
  661 AGCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT GTTGCGCCTC AATAGCCCTC
  721 AGCAAATTGT TCTGCTGCTG CACTATACCA GACAATAATT GTCTGGCCTG TACCGTCAGC
  781 GTCATTGACG CTGCGCCCAT AGTGCTTCCT GCTGCTCCCN ANAACCCAAG GAACAAAGCT
  841 CCNATTCCCA CTNCNCTTTT TTTCTCTCTG CACCACTCTT CTCTTNGCCT NGGNGGGNGC
  901 TACNNCNAAN NGTTCAATTT TTACNACTTT ATATTTATAT NATTNNCTTC NCCNATTNNC
961 CCNNATATCT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.
user report Hashizume, O., Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci. Rep. 5: 10434 (2015). PMID 26000717.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl