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CSII-EF-RfA-IRES-Puro (#RDB12869)

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with puromycin resistance marker.


Drawn by SnapGene® software
Sequence (full) RDB12869hts01.seq
Publication Kurita, R., PLoS One 8 (3): e59890 (2013). [link to RRC of NBRP]
Test sheet RDB12869_B1Afp1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with puromycin resistance marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-RfA-IRES-Puro (Plasmid)
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12869 CSII-EF-RfA-IRES-Puro DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-EF-RfA-IRES-Puro was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12869).

Reference section:

Kurita, R., Suda, N., Sudo, K., Miharada, K., Hiroyama, T., Miyoshi, H., Tani, K., Nakamura, Y., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656. [link to RRC of NBRP]
user_report Onuma, S., The Lack of Bmal1, a Core Clock Gene, in the Intestine Decreases Glucose Absorption in Mice. Endocrinology 163 (9): bqac119 (2022). PMID 35904419. [link to RRC of NBRP]
user_report Kawai, M., Lack of PTEN in osteocytes increases circulating phosphate concentrations by decreasing intact fibroblast growth factor 23 levels. Sci. Rep. 10 (1): 21501 (2020). PMID 33299044. [link to RRC of NBRP]
user_report Hashizume, O., Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci. Rep. 5: 10434 (2015). PMID 26000717. [link to RRC of NBRP]