Resource data sheet
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CSII-EF-GFP-IRES-Puro

Lentivirus vector expressing GFP driven by EF-1 alpha promoter with puromycin resistance marker.

Catalog number RDB12868
Resource name CSII-EF-GFP-IRES-Puro
Clone info. Lentivirus vector expressing GFP driven by EF-1 alpha promoter with puromycin resistance marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-RfA-IRES-Puro (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12868 CSII-EF-GFP-IRES-Puro DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12868_A9Gtp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: EF1a pro.,attB1,EGFP5' | Primer: EF1a-C_F | Sequence: RDB12868_A9Gta.seq
>12868_12868_A9Gt_3_EF1a-C_F_C11_08_ABI24.ab1
    1 NNNNNNNNNN NNTTTTGANT TNGGNNCTTG GTTCNTTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCNTTTC AGGTGTCGTG AACACGCTAC CGGTCTCGAG AATTATCACA
  121 AGTTTGTACA AAAAAGCAGG CTCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG
  181 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC
  241 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC
  301 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC
  361 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA
  421 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC
  481 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC
  541 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC
  601 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC
  661 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
  721 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC
781 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGANTTCG TGACCGCCGC CGG
//
Region: EGFP3',attB2,chimeric intron,IRES | Primer: mVenus-M_F | Sequence: RDB12868_A9Gtb.seq
>12868_12868_A9Gt_3_mVenus-M_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT GNACTTCNNG ATCCGCCACA ACATCGAGGA
   61 CGGCAGCGTG CAGCTCGCCG ACNACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
  121 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
  181 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT
  241 GGACGAGCTG TACAAGTCCG GACTCAGATC CTACTAGTAG GATCTCGAGA TATCTAGACC
  301 CAGCTTTCTT GTACAAAGTG GTGATAATTC TGCAGCGGCC GCGGATCCTC TAGACTGGAA
  361 TTAATTCGCT GTCTGCGAGG GCCAGCTGTT GGGGTGAGTA CTCCCTCTCA AAAGCGGGCA
  421 TGACTTCTGC GCTAAGATTG TCAGTTTCCA AAAACGAGGA GGATTTGATA TTCACCTGGC
  481 CCGCGGTGAT GCCTTTGAGG GTGGCCGCGT CCATCTGGTC AGAAAAGACA ATCTTTTTGT
  541 TGTCAAGCTT GAGGTGTGGC AGGCTTGGGA TCTGGCCATA CACTTGAGTG ACAATGACAT
  601 CCACTTTGCC TTTCTCTCCA CAGGTGTCCA CTCCCAGGTC CAACTGCAGG TCGAGCATGC
661 ATCTAGGGCG GCCAATTCCG CCCCTCTCCC CNNNNNNN
//
Region: CMV pro.,5'LTR,HIV-1 psi | Primer: CMV_Forward | Sequence: RDB12868_A9Gtc.seq
>12868_12868_A9Gt_3_CMV-Forward_C11_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTG NNNCNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC
  121 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC
  181 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT
  241 AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA
  301 ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA
  361 GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA
  421 TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG
  481 GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA
  541 GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT
  601 ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA
  661 TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC
  721 TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGGGCCGCT
  781 NATCTTCAGA CCTGGAGGAN GAGATATGAG GGANNATTGG AGAAGTGAAT TATATAAATA
841 TNAAGTAGTA AAAAANTGAN NCNTTNNGAG TAGCACCCNC CNA
//
Region: WPRE,PuroR,IRES | Primer: PRE_rev | Sequence: RDB12868_A9Gtd.seq
>12868_12868_A9Gt_3_PRE_rev_B11_05_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNGN NCNNNNTAGT TAAGAATACC NGTCAATCTT TCACAAATTT
   61 TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG ATGTTTCAGG CACCGGGCTT
  121 GCGGGTCATG CACCAGGTGC GCGGTCCTTC GGGCACCTCG ACGTCGGCGG TGACGGTGAA
  181 GCCGAGCCGC TCGTAGAAGG GGAGGTTGCG GGGCGCGGAG GTCTCCAGGA AGGCGGGCAC
  241 CCCGGCGCGC TCGGCCGCCT CCACTCCGGG GAGCACGACG GCGCTGCCCA GACCCTTGCC
  301 CTGGTGGTCG GGCGAGACGC CGACGGTGGC CAGGAACCAC GCGGGCTCCT TGGGCCGGTG
  361 CGGCGCCAGG AGGCCTTCCA TCTGTTGCTG CGCGGCCAGC CGGGAACCGC TCAACTCGGC
  421 CATGCGCGGG CCGATCTCGG CGAACACCGC CCCCGCTTCG ACGCTCTCCG GCGTGGTCCA
  481 GACCGCCACC GCGGCGCCGT CGTCCGCGAC CCACACCTTG CCGATGTCGA GCCCGACGCG
  541 CGTGAGGAAG AGTTCTTGCA GCTCGGTGAC CCGCTCGATG TGGCGGTCCG GGTCGACGGT
  601 GTGGCGCGTG GCGGGGTAGT CGGCGAACGC GGCGGCGAGG GTGCGTACGG CCCGGGGGAC
  661 GTCGTCGCGG GTGGCGAGGC GCACCGTGGG CTTGTACTCG GTCATGGAAG GTCGTCTCCT
  721 TGTGGGTTGT GGCAAGCTTA TCATCGTGTT TTTCAAAGGA AAACCACGTC CCCGNGGTTC
  781 GGGNGGCCTA GACGTTTTTT TAACCTCGAC TAAACACATG TAAAGCATGT GCACCGAGGC
841 CCCAGNTCAG ATCCNATACA ANGGGGNTNN CTTCNGGGCN TCCNNCAGCC CC
//
Region: f1ori,bGHp(A),3'LTR | Primer: f1ori_R3 | Sequence: RDB12868_A9Gte.seq
>12868_12868_A9Gt_3_f1ori_R3_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TNNNCCTCAN AAGCCNTAGA GCCCNNCCGC
   61 ATCCCCAGCA TGCCTGCTAT TGTCTTCCCA ATCCTCCCCC TTGCTGTCCT GCCCCACCCC
  121 ACCCCCCAGA ATAGAATGAC ACCTACTCAG ACAATGCGAT GCAATTTCCT CATTTTATTA
  181 GGAAAGGACA GTGGGAGTGG CACCTTCCAG GGTCAAGGAA GGCACGGGGG AGGGGCAAAC
  241 AACAGATGGC TGGCAACTAG AAGGCACAGT CGAGGCTGAT CAGCGGGTTT AAACGGGCCC
  301 TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC
  361 ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA
  421 GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC
  481 CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA TGCTAGGCGG CTGTCAAACC
  541 TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG CAGGCTCACA GGGTGTAACA
  601 AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC TTGCTCAACT GGTACTAGCT
  661 TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG CCCTGGTGTG TAGTTCTGCC
  721 AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC AAGGATATCT TGTCTTCGTT
  781 GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTNA AAAAGTGGCT AAGATCTACA
  841 GCTGCCTTGT AAGTCATTGG NCTTAAAGGT ACCTGANGTG TGACTGGAAA ANCCNCCTCC
  901 TCCTCCTCTT GTGCTTCTAG CCAGGCACNA TCAGCATTGG NAGCTGCTGT ATTGCTACTT
  961 GNGANTGCTC CNTGTTTTTC TAGGTCTCGA CGGNNNCNAT GNGGGGANGC NGNCNNAANG
 1021 GANNNNCNNN NNNNTCNNNN GGNNNNANNN NNNNNNNCNN NANNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNN
//
Region: EF1a pro.,cPPT/CTS,RRE | Primer: EF1a-N_R | Sequence: RDB12868_A9Gtf.seq
>12868_12868_A9Gt_3_EF1a-N_R_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGCG NNGTGCGCTC TGCCNNCTGN CGGGCACCGG ANCCTCACGA
   61 AGCTTGAATT AATTCAAAGT GAATTTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA
  121 TTTTTGTAAT TTGTTTTTGT AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA
  181 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA
  241 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT
  301 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC
  361 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT
  421 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT
  481 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA
  541 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG
  601 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA
  661 TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGAGCAACCC
  721 CAAATCCCCA GGAGCTGTTG ATCCTTTANG TATCTTTCCA CAGCCAGGAT TCTTGCCTGG
  781 ANCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT GTTGCGCCTC AATAGCCCTC
841 ANCNNATTGT TCTGCTGCTG CAC
//
Region: SV40pro/ori,Zeo,SV40p(A) | Primer: SV40pro_F | Sequence: RDB12868_A9Gtg.seq
>12868_12868_A9Gt_3_SV40pro_F_F11_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNG NNGNTTTTTT GGANGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGNNCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATANCGTCNA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGTGTGA ANTTGTTATC CGCTCACAAT TCCNCACAAC
901 ATACGANCCN GAAGCATAAA GNGTAAAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

GNP_filter3_RDBDEP_html_190927.pl