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GFP-NFATc4(FL)/pCAGEN (#RDB12860)

Expression clone of mouse Nfatc4 tagged with GFP.

Clone info. The plasmid expressing mouse GFP-tagged NFATc4 under the control of the CAG promoter. An oligonucleotide encoding GFP sequence was fused to the 5' end of full length NFATc4 coding sequence.
Comment Expression was confirmed by the depositor with anti-NFATc4 and anti-GFP antibodies, respectively. Please refer Fig.3 of Kurabayashi, N., Genes Dev. 27 (24): 2708-2721, 2013.
Vector backbone pCAGEN (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Nfatc4 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kurabayashi, Nobuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12860 GFP-NFATc4(FL)/pCAGEN DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The GFP-NFATc4(FL)/pCAGEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12860).

Reference section:

Kurabayashi, N., Sanada, K., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12860_A4Iop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C
Sequence file: RDB12860_A4Ioa.seq check
>12860_12860_A4Io_EGFP-C_G11_20.ab1
    1 TCAGGGGACA TCTTCGGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTCGAGCTAT
   61 GGGGGCCGCA AGCTGCGAGG ATGAGGAGCT GGAATTTAAG CTGGTGTTCG GAGAGGAAAA
  121 GGAGCCCCCC CCGCTGGGCC CTGGGGGTCC TGGGGAAGAG CTGGACTCAG AGGATACCCC
  181 TCCATGCTGT CGTTTGGCCC TGGGGGAACC CCTTCCTTAT GGTGCTGCCC CTATTGGGAT
  241 TCCCCGACCC CCACCCCCTC GGCCAGGGAT GCACTCACCA CCGCCCCGTC CCGCCCCTTC
  301 ACCTGGCACC TGGGAGAGCC AGCCAGCTAG GTCGGTGAGG CTGGGGGGCC CAGGAGGTAA
  361 TGCAGGGGGT GCTGGGGGTG GTCGTGTTCT TGAGTGTCCC AGCATCCGGA TTACCTCCAT
  421 CTCTCCCACG CCTGACCCGC CGACCTCGCT AGAGGACACA TCTGAAACTT GGGGGGACGG
  481 CTCTCCTAGA GATTACCCTC CACCAGAAGG CTTTGGGGGC TATCGAGAGG CTGGAGGTCA
  541 GGGCGGAGGC GCTTTCTTCA GCCCGAGCCC TGGCAGCAGC AGCCTGTCTT CGTGGAGCTT
  601 CTTCTCGGAC GCCTCCGACG AGGCAGCCCT GTATGCAGCG TGCGACGAGG TGGAGTCTGA
  661 ACTTAATGAA GCAGCCTCCC GTTTTGGCTT GAGCTCTCCG CTGCCCTCGC CCCGGGCTTC
  721 TCCTAGGCCA TGGACCCCGG AGGATCCCTG GAGCCTATAC GGTCCAAGCT CGGGAGGCCG
  781 AGCGCCAGAG GATAGCTGGC TACTCCTCAG CGCTCCTGGG CCCGTCCCAG CCTCCCCTCG
  841 GCCTGCTTCT CCCTGCGGCA AGCGGCGCTA TTCCAGTTCC GGAACCCCAT CTTCGGCCTC
  901 CCCAGCTTTG TCCCGCCGAG GCAGCCTTGG GGAAGAAGGT CCAGAGCCAC CTCCACCACC
  961 CCCGTTGCCT CTGGTCCCGG ACCTAGTTCC CCTGGCCCCT TTTGACTATG TGGTGCTCAC
 1021 ACGAGAGCAT CCCTCAGAAA CTCGGAAGAC TCTAGCGAGC AGGCAGTGTC CTGCTTCGGT
1081 CTGGAGAGCT CTCGTGCATG AAGCCTACCC TTCGTTACGA GCACTCTGGT GGGCATGC
//
Primer: pCA-bgter
Sequence file: RDB12860_A4Iob.seq check
>RDB12860_1_pCA_bgter_A11_02.ab1
    1 AGCCAAGAAG TCCAATAAAT TTTTGGCAGA GGGAAAAAGA TCTCAGTGGT ATTTGTGAGC
   61 CAGGGCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT GAGGAGTGCG
  121 GCCGCGATGG TACCGTCGAC TAAGCTTGCG GCCGCTCAGG CAGGAGGCTC TTCTCCAGGA
  181 CGTGCAGGGA AGCCACTCAG GTCTCGGCCA ATGATCTCAC TCACTTCCTC CAGGGTGATA
  241 CCCTGGATAG GTACACTGTC TCTGAAGCCG CTGCTGTAGC CACCCCTGGC TTTCTCCTGC
  301 TCCGAAGCCC CCTCCCCAGG GGTATAGCCT GGCCCCACCT CATTGTAGCC CTCAGGAGGT
  361 AAGGGGTGGA TGGCACTCTG GGGATGGAAG GGGTCTTCGA GTGGTGGGGA GGAAGGCAGT
  421 GGCGGCCTAA AGGGGGCTGG AGGGGAGAAT GGAAGCCCCA GGGCAAAAGA GGAGCCCTGT
  481 CCTCCATATT GATCACCAGG AAAGGGGCGC GGAAGGAAAG AGACTGAAGG TAGGCGGGCA
  541 CAACCTGTGG TACCCCCAGT CTCAGGGAAC ATCCGCAGGC CAGATCTATA GGACGGTGGG
  601 GGCCCCGTCT GGGGGTACAG AGCGGGTGTG CTATAACCAA AGCCTTCTGA CAGATAAGGA
  661 GTCTCATAAG CAGGGTCTTC ATGGGGATAG GAGGGGTAAG GGGGCCTGGG CGGTGAGAAG
  721 TCCACGTCAG GGCCAAAGGG GGGACCCGAT GTGGAAGGGA AGCCCCGGAG AGATGAGTCT
  781 GGTAGGGGCT CCTCCTTGAA GACCACAGGT AGGAACTTGA AACTTTGGGT AGGACTGCGC
  841 TTCCTCCGTC CATTGGAGAC GTAAAAGTAG ACCTGGACTG GTCGGGACAC CCGCTTGTTG
  901 CTGTACTCGG GGATGGTGAG AGTCAATGTC ACCTCACTGC TCTGCAGTCG GGTTCACCGC
  961 TGCCTCTTCC TCCCACTGCA GTTTTCCATC AGACCCCTCT CAATGAACAC CACTTTAGAG
 1021 TCTGGCAGGA AGTGGACCAG TCAACACTAG CTCTCTCTCT CTCACAGAGC AGCACTGGCT
 1081 GTAGTCTCAC TGGGGCAGCT CTGGGCGACG CTGGAGCACT CGAATGGACA CTGATGCCTG
1141 GCTGCACGAA CACGAAACCA TTTCCG
//
Primer: pAxCA_F1
Sequence file: RDB12860_A4Ioc.seq check
>RDB12860_1_pAxCA_F1_H10_22.ab1
    1 CGGTCCTTGT CTACATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
   61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GAGGATCCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG
  841 ACTCAGATCT CGAGCTATGG GGGCCGCAAG CTGCGAGGAT GAGGAGCTGG AATTTAAGCT
  901 GGTGTTCGGA GAGGAAAAGG AGCCCCCCCC CGCTGGGCCC TGGGGGTCCT GGGGAAAGAG
  961 CTGGACTCAG AGGATACCCC TCCATGCTGT CGTTTGGCCC TGGGGGGAAC CCCTTTCCTA
 1021 TGGTGCTGCC CCTATTGGGA TTCCCCGACC CCACCCCTCG TCAGGAATGC ACTCACACGG
 1081 CCCCGGTTCC CGCCCCATCA CTGCACCTGG GAGAGTCAGG CCAGCTTAGG TTCGTGAGTC
1141 CTGAGTCAGA GTTAATTGCC AATGGAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]