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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

NFATc4(DN)/pCAGEN (#RDB12859)

Expression clone of mouse Nfatc4 dominant negative form

Clone info. The plasmid expressing dominant-negative (DN) form of mouse NFATc4 under the control of the CAG promoter.
Comment Encoding 1-191aa of mouse Nfatc4. Expression was confirmed by the depositor with anti-NFATc4 antibody.
Vector backbone pCAGEN (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Nfatc4 cDNA
Depositor|Developer Kurabayashi, Nobuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12859 NFATc4(DN)/pCAGEN DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The NFATc4(DN)/pCAGEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12859).

Reference section:

Kurabayashi, N., Sanada, K., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12859_A4Iop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 ()
Sequence file: RDB12859_A4Ioa.seq check
>RDB12859_1_pAxCA_F1_E10_13.ab1
    1 CATGGAGAAA ACATTCTGGC TCAATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG
   61 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGCCA CCATGGGGGC
  121 CGCAAGCTGC GAGGATGAGG AGCTGGAATT TAAGCTGGTG TTCGGAGAGG AAAAGGAGCC
  181 CCCCCCGCTG GGCCCTGGGG GTCCTGGGGA AGAGCTGGAC TCAGAGGATA CCCCTCCATG
  241 CTGTCGTTTG GCCCTGGGGG AACCCCTTCC TTATGGTGCT GCCCCTATTG GGATTCCCCG
  301 ACCCCCACCC CCTCGGCCAG GGATGCACTC ACCACCGCCC CGTCCCGCCC CTTCACCTGG
  361 CACCTGGGAG AGCCAGCCAG CTAGGTCGGT GAGGCTGGGG GGCCCAGGAG GTAATGCAGG
  421 GGGTGCTGGG GGTGGTCGTG TTCTTGAGTG TCCCAGCATC CGGATTACCT CCATCTCTCC
  481 CACGCCTGAC CCGCCGACCT CGCTAGAGGA CACATCTGAA ACTTGGGGGG ACGGCTCTCC
  541 TAGAGATTAC CCTCCACCAG AAGGCTTTGG GGGCTATCGA GAGGCTGGAG GTCAGGGCGG
  601 AGGCGCTTTC TTCAGCCCGA GCCCTGGCAG CAGCAGCCTG TCTTCGTGGA GCTTCTTCTC
  661 GGACGCCTCC GACGAGGCAG CCCTGTGAGC GGCCGCACTC CTCAGGTGCA GGCTGCCTAT
  721 CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCTTTTT
  781 CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA
  841 TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA
  901 AGGACATATG GGAGGGCAAA TCATTTAAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT
  961 GGCAACATAT GCCCATATGC TGGCTGCCAT GAACAAGGTT GGCTATAAAG AGGTCATCAG
 1021 TATATGAAAC AGCCCCCTGC TGTCCATTCC CTTATTCCAT AGAAAAGCCT GACTGAGGTA
 1081 GATTTTTTTT TATATTTGTT TGTGTATTTT TCTTAACATC CTAATTTCGT ACATGTTTTA
1141 CTAGCAGAAA TTTTCCCTTC CTCTTCCCTT GA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]