Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

NFATc4(CA)/pCAGEN (#RDB12858)

Expression clone of mouse Nfatc4 constitutively-active form

Clone info. The plasmid expressing constitutively-active (CA) form of mouse NFATc4 under the control of the CAG promoter.
Comment Encoding 317-902aa of mouse Nfatc4. Expression was confirmed by the depositor with anti-NFATc4 antibody.
Vector backbone pCAGEN (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Nfatc4 cDNA
Depositor|Developer Kurabayashi, Nobuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12858 NFATc4(CA)/pCAGEN DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The NFATc4(CA)/pCAGEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12858).

Reference section:

Kurabayashi, N., Sanada, K., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12858_A4Iop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 ()
Sequence file: RDB12858_A4Ioa.seq check
>RDB12858_2_pAxCA_F1_C10_07.ab1
    1 GCACTATGTA GATCTGCTAC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA
   61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCGCCACCA TGTATGTGGG
  121 TGCTCCACCA ACCGAGAGCA TCCCTCAGAA AACTCGGAGG ACTTCTAGCG AGCAAGCAGT
  181 GGCCCTGCCT CGGTCTGAAG AGCCTCCCTC GTGCAATGGG AAGCTACCCT CCGGTACAGA
  241 GGACTCTGTG GCTGCTCCGG GGGCTCTGCG GAAAGAGGTG GCCGGCATGG ATTACCTAGC
  301 AGTGCCTTCT CCCCTTGCTT GGTCCAAGGC CCGGATTGGG GGACACAGCC CCATCTTCAG
  361 GACCTCTGCC CTACCTCCCC TGGACTGGCC TTTACCCAGC CAGTATGAGC AGCTGGAGCT
  421 GAGGATCGAG GTACAGCCTA GAGCACACCA CCGAGCTCAC TATGAGACTG AGGGCAGCCG
  481 GGGGGCTGTC AAGGCTGCTC CTGGAGGCCA CCCTGTGGTC AAGCTCCTAG GCTACAGTGA
  541 GAAGCCATTG ACTCTGCAGA TGTTCATTGG CACTGCAGAT GAGAGGAGCC TGCGGCCCCA
  601 TGCCTTCTAC CAGGTGCACC GGATTACTGG CAAGATGGTG GCTACAGCCA GCTATGAAGC
  661 TGTAGTCAGT GGTACCAAAG TGTTGGAGAT GACCTTACTC CCAGAGAACA ACATGGCTGC
  721 CAACATTGAC TGTGCTGGAA TCCTGAAGCT TCGAAATTCA GACATTGAGC TACGGAAGGG
  781 TGAGACAGAC ATCGGGCGCA AAACCACGCG TGTGCGGCTG GTGTTCCGTG TGCACGTGCC
  841 TCAGGCGGCG GGAAGGTCGT GTCTGTGCAG GCAGCATCAG TGCCCATCGA GTGCTCCCAG
  901 CGCTCGGCCC AGGAGCTGCC CCAGGTGGAG ACCTACAGCC CCAGTGCCTG CTCTGTGAGA
  961 GGAGGGAGGA GCTAGTGTTG ACTGGTTCCA CTTCCTGCAG ACTCTAAAGT GGTGTCATTG
 1021 AGAGGGGTCT GATGGAAAAC TGCATGGGGA GGAAGAGCAG CGGTGACGAC TGCAGAGCAT
 1081 GAGGTGACAT GACTCTCACA TTCTCGAGTA CAGCACAGCG GGGGTCGGAC AGTCAGGCTT
1141 ACATTTACGT CTTCCCAATG GACGGGAGGA AAA
//
Primer: pCA_bgter ()
Sequence file: RDB12858_A4Iob.seq check
>RDB12858_2_pCA_bgter_D10_10.ab1
    1 ACCCAGCAGG TCCAATAATT TTTGGCAGAG GGAAAAGATC TCAGTGGTAT TTGTGAGCCA
   61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGCGGC
  121 CGCTCAGGCA GGAGGCTCTT CTCCAGGACG TGCAGGGAAG CCACTCAGGT CTCGGCCAAT
  181 GATCTCACTC ACTTCCTCCA GGGTGATACC CTGGATAGGT ACACTGTCTC TGAAGCCGCT
  241 GCTGTAGCCA CCCCTGGCTT TCTCCTGCTC CGAAGCCCCC TCCCCAGGGG TATAGCCTGG
  301 CCCCACCTCA TTGTAGCCCT CAGGAGGTAA GGGGTGGATG GCACTCTGGG GATGGAAGGG
  361 GTCTTCGAGT GGTGGGGAGG AAGGCAGTGG CGGCCTAAAG GGGGCTGGAG GGGAGAATGG
  421 AAGCCCCAGG GCAAAAGAGG AGCCCTGTCC TCCATATTGA TCACCAGGAA AGGGGCGCGG
  481 AAGGAAAGAG ACTGAAGGTA GGCGGGCACA ACCTGTGGTA CCCCCAGTCT CAGGGAACAT
  541 CCGCAGGCCA GATCTATAGG ACGGTGGGGG CCCCGTCTGG GGGTACAGAG CGGGTGTGCT
  601 ATAACCAAAG CCTTCTGACA GATAAGGAGT CTCATAAGCA GGGTCTTCAT GGGGATAGGA
  661 GGGGTAAGGG GGCCTGGGCG GTGAGAAGTC CACGTCAGGG CCAAAGGGGG GACCCGATGT
  721 GGAAGGGAAG CCCCGGAGAG ATGAGTCTGG TAGGGGCTCC TCCTTGAAGA CCACAGGTAG
  781 GAACTTGAAA CTTTGGGTAG GACTGCGCTT CCTCCGTCCA TTGGAGACGT AAAAGTAGAC
  841 CTGGACTGGT CGGGACACCC GCTTGTTGCT GTACTCGGGG ATGGTGAGAG TCAATGTCAC
  901 CTCACTGCTC TGCAGTCGGT TCACCGCTGC CTCTTCCTCC CACTGCAGTT TTCCATCAGG
  961 ACCCCTCTCA ATGAACACCA CTTTAGAGTC TGGCAGGAAG TTGGAACCAG TCAACACTAG
 1021 CTCCTCCTCC TCCTCTCACA GAGCAGGCAC TGGGGGCTGT AGTCTCCACC TGGGGGCAGC
 1081 TCTGGTGAGC GGCTGGGAGC ACTCGATGGT CACTGAATGC TGGCTGGCAC AGGAAACACG
1141 GACCCATTCC CCGCCGATCC TGGAAGGCAC ACA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]