Please review the QC test results indicated by icon below as well as clone information before placing your order.

NFATc4(CA)/pCAGEN

Expression clone of mouse Nfatc4 constitutively-active form

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12858_A4Iop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB12858_A4Ioa.seq
>RDB12858_2_pAxCA_F1_C10_07.ab1     1 GCACTATGTA GATCTGCTAC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA    61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCGCCACCA TGTATGTGGG   121 TGCTCCACCA ACCGAGAGCA TCCCTCAGAA AACTCGGAGG ACTTCTAGCG AGCAAGCAGT   181 GGCCCTGCCT CGGTCTGAAG AGCCTCCCTC GTGCAATGGG AAGCTACCCT CCGGTACAGA   241 GGACTCTGTG GCTGCTCCGG GGGCTCTGCG GAAAGAGGTG GCCGGCATGG ATTACCTAGC   301 AGTGCCTTCT CCCCTTGCTT GGTCCAAGGC CCGGATTGGG GGACACAGCC CCATCTTCAG   361 GACCTCTGCC CTACCTCCCC TGGACTGGCC TTTACCCAGC CAGTATGAGC AGCTGGAGCT   421 GAGGATCGAG GTACAGCCTA GAGCACACCA CCGAGCTCAC TATGAGACTG AGGGCAGCCG   481 GGGGGCTGTC AAGGCTGCTC CTGGAGGCCA CCCTGTGGTC AAGCTCCTAG GCTACAGTGA   541 GAAGCCATTG ACTCTGCAGA TGTTCATTGG CACTGCAGAT GAGAGGAGCC TGCGGCCCCA   601 TGCCTTCTAC CAGGTGCACC GGATTACTGG CAAGATGGTG GCTACAGCCA GCTATGAAGC   661 TGTAGTCAGT GGTACCAAAG TGTTGGAGAT GACCTTACTC CCAGAGAACA ACATGGCTGC   721 CAACATTGAC TGTGCTGGAA TCCTGAAGCT TCGAAATTCA GACATTGAGC TACGGAAGGG   781 TGAGACAGAC ATCGGGCGCA AAACCACGCG TGTGCGGCTG GTGTTCCGTG TGCACGTGCC   841 TCAGGCGGCG GGAAGGTCGT GTCTGTGCAG GCAGCATCAG TGCCCATCGA GTGCTCCCAG   901 CGCTCGGCCC AGGAGCTGCC CCAGGTGGAG ACCTACAGCC CCAGTGCCTG CTCTGTGAGA   961 GGAGGGAGGA GCTAGTGTTG ACTGGTTCCA CTTCCTGCAG ACTCTAAAGT GGTGTCATTG  1021 AGAGGGGTCT GATGGAAAAC TGCATGGGGA GGAAGAGCAG CGGTGACGAC TGCAGAGCAT  1081 GAGGTGACAT GACTCTCACA TTCTCGAGTA CAGCACAGCG GGGGTCGGAC AGTCAGGCTT 1141 ACATTTACGT CTTCCCAATG GACGGGAGGA AAA //
Primer: pCA_bgter Sequence file: RDB12858_A4Iob.seq
>RDB12858_2_pCA_bgter_D10_10.ab1     1 ACCCAGCAGG TCCAATAATT TTTGGCAGAG GGAAAAGATC TCAGTGGTAT TTGTGAGCCA    61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGCGGC   121 CGCTCAGGCA GGAGGCTCTT CTCCAGGACG TGCAGGGAAG CCACTCAGGT CTCGGCCAAT   181 GATCTCACTC ACTTCCTCCA GGGTGATACC CTGGATAGGT ACACTGTCTC TGAAGCCGCT   241 GCTGTAGCCA CCCCTGGCTT TCTCCTGCTC CGAAGCCCCC TCCCCAGGGG TATAGCCTGG   301 CCCCACCTCA TTGTAGCCCT CAGGAGGTAA GGGGTGGATG GCACTCTGGG GATGGAAGGG   361 GTCTTCGAGT GGTGGGGAGG AAGGCAGTGG CGGCCTAAAG GGGGCTGGAG GGGAGAATGG   421 AAGCCCCAGG GCAAAAGAGG AGCCCTGTCC TCCATATTGA TCACCAGGAA AGGGGCGCGG   481 AAGGAAAGAG ACTGAAGGTA GGCGGGCACA ACCTGTGGTA CCCCCAGTCT CAGGGAACAT   541 CCGCAGGCCA GATCTATAGG ACGGTGGGGG CCCCGTCTGG GGGTACAGAG CGGGTGTGCT   601 ATAACCAAAG CCTTCTGACA GATAAGGAGT CTCATAAGCA GGGTCTTCAT GGGGATAGGA   661 GGGGTAAGGG GGCCTGGGCG GTGAGAAGTC CACGTCAGGG CCAAAGGGGG GACCCGATGT   721 GGAAGGGAAG CCCCGGAGAG ATGAGTCTGG TAGGGGCTCC TCCTTGAAGA CCACAGGTAG   781 GAACTTGAAA CTTTGGGTAG GACTGCGCTT CCTCCGTCCA TTGGAGACGT AAAAGTAGAC   841 CTGGACTGGT CGGGACACCC GCTTGTTGCT GTACTCGGGG ATGGTGAGAG TCAATGTCAC   901 CTCACTGCTC TGCAGTCGGT TCACCGCTGC CTCTTCCTCC CACTGCAGTT TTCCATCAGG   961 ACCCCTCTCA ATGAACACCA CTTTAGAGTC TGGCAGGAAG TTGGAACCAG TCAACACTAG  1021 CTCCTCCTCC TCCTCTCACA GAGCAGGCAC TGGGGGCTGT AGTCTCCACC TGGGGGCAGC  1081 TCTGGTGAGC GGCTGGGAGC ACTCGATGGT CACTGAATGC TGGCTGGCAC AGGAAACACG 1141 GACCCATTCC CCGCCGATCC TGGAAGGCAC ACA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl